Browsing by Author "Alp, H. H."

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Comparison of Icodextrin With Thymoquinone: a New Hope for Postoperative Adhesions
(Medknow Publications & Media Pvt Ltd, 2017) Yilmaz, O.; Kiziltan, R.; Celik, S.; Yildirm, S.; Alp, H. H.; Aras, A.; Kotan, C.
Objective: The purpose of this study was to compare the effects of thymoquinone and icodextrin in rats within the framework of an experimental adhesion model. Materials and Methods: Rats were separated into three groups: (1) a control group consisting of rats that had 2 ml of isotonic solution administered intraperitoneally, (2) an ICO group administered with 2 ml of 4% icodextrin, and (3) a TQ group administered thymoquinone (10 mg/kg), all following cecal abrasion. The three groups underwent a reoperation on the 7th postoperative day. Hydroxyproline levels were analyzed in the resected adhesive tissues, and histopathological investigations were conducted. Blood samples were collected for biochemical analyses. Results: Fewer postoperative adhesions were observed in the ICO and TQ groups compared with the control group. A comparison of the TQ and ICO groups revealed lowers levels of postoperative adhesions in the TQ group. Compared with the control group, malondialdehyde, 8-OH-deoxyguanosine/deoxyguanosine (8-OHdG/10dG), Coenzyme Q10 (CoQ10), and CoenzymeQ10/reduced CoenzymeQ10 (CoQ10/CoQ10H) values were found to be lower in the TQ and ICO groups. When the TQ and ICO groups were compared with respect to their biochemical parameters, the results for all of the four parameters were found to be statistically significantly lower in the TQ group (P < 0.000). The levels of hydroxyproline in the control, ICO, and TQ groups were found to be (mean +/- standard deviation) 502.25 +/- 90.39 mu g/g, 342.13 +/- 66.61 mu g/g, and 287.88 +/- 49.59 mu g/g, respectively. Conclusions: A comparison of the antiadhesive effects of thymoquinone and icodextrin revealed thymoquinone to be more effective. These results indicate that thymoquinone is an efficient and strong antiadhesive molecule.
The Effect of Pde5 Inhibitors on Bone and Oxidative Damage in Ovariectomy-Induced Osteoporosis
(Wiley-blackwell, 2016) Alp, H. H.; Huyut, Z.; Yildirim, S.; Basbugan, Y.; Ediz, L.; Sekeroglu, M. R.
The Impact of High Fructose on Cardiovascular System: Role of Α-Lipoic Acid
(Sage Publications Ltd, 2016) Saygin, M.; Asci, H.; Cankara, F. N.; Bayram, D.; Yesilot, S.; Candan, I. A.; Alp, H. H.
The aim of this study was to evaluate the role of alpha-lipoic acid (alpha-LA) on oxidative damage and inflammation that occur in endothelium of aorta and heart while constant consumption of high-fructose corn syrup (HFCS). The rats were randomly divided into three groups with each group containing eight rats. The groups include HFCS, HFCS + alpha-LA treatment, and control. HFCS was given to the rats at a ratio of 30% of F30 corn syrup in drinking water for I 0 weeks. alpha-LA treatment was given to the rats at a dose of 100 mg/kg/day orally for the last 6 weeks. At the end of the experiment, the rats were killed by cervical dislocation. The blood samples were collected for biochemical studies, and the aortic and cardiac tissues were collected for evaluation of oxidant-antioxidant system, tissue bath, and pathological examination. HFCS had increased the levels of malondialdehyde, creatine kinase MB, lactate dehydrogenase, and uric acid and showed significant structural changes in the heart of the rats by histopathology. Those changes were improved by alpha-LA treatment as it was found in this treatment group. Immunohistochemical expressions of tumor necrosis factor cc and inducible nitric oxide synthase were increased in HFCS group, and these receptor levels were decreased by alpha-LA treatment. All the tissue bath studies supported these findings. Chronic consumption of HFCS caused several problems like cardiac and endothelial injury of aorta by hyperuricemia and induced oxidative stress and inflammation. alpha-LA treatment reduced uric acid levels, oxidative stress, and corrected vascular responses. alpha-LA can be added to cardiac drugs due to its cardiovascular protective effects against the cardiovascular diseases.
May Supplementation of Coenzyme Q10 Help Prevent Development of Hydatidiform Mole
(7847050 Canada inc, 2017) Kolusari, A.; Cebi, A.; Akgun, E.; Alp, H. H.; Bakan, E.
Objective: The pathological mechanisms of gestational trophoblastic disease have not yet been clearly determined. It is thought that oxidative damage contributes to the process. The aim of this study was to determine the levels of coenzyme Q10 (CoQ10), DNA damage, and lipid peroxidation in patients with hydatidiform mole. Materials and Methods: The authors studied the levels of CoQ10, 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA) by high-performance liquid chromatography (HPLC), and the activity of glutathione peroxidase (GPX) by spectrophotometric method in blood obtained from patients with a complete hydatidiform mole (n=29), healthy pregnant women (n=29), and healthy non-pregnant women (n=29). Results: The 8-OHdG/dG ratio (2.8148 +/- 0.81592) and MDA (10.8341 +/- 4.64875 mu mol) were significantly higher in patients with complete hydatidiform mole, while the ubiquinol-10/ubiquinone-10 ratio (0.2107 +/- 0.15675) and GPX activity (43.4606 +/- 18.31694 mU/ml) were lower (p < 0.001). Conclusion: The authors suggest that both mitochondrial oxidative and oxidative DNA damage play important roles in the pathogenesis of complete hydatidiform mole. Therefore supplementation of CoQ10 prevents recurrent gestational trophoblastic disease.