Browsing by Author "Alp, H.H."

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Actions of Resveratrol and Serotonin Added To Stored Bloods on Lipid Peroxidation and Oxidative Dna Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) Huyut, Z.; Şekeroğlu, M.R.; Alp, H.H.; Balahoroğlu, R.; Çokluk, E.
Stored blood for use in diseases such as acute blood loss, injury and anemia can be maintained for about a month. It was reported that some negative changes called "storage lesions" occur in stored bloods time dependently. In order to delay these changes, preservatives are added to stored bloods. In this study, we aimed to determine the effects of resveratrol and serotonin on oxidative DNA and mitochondrial damage and lipid peroxidation in stored blood. Blood was taken from 10 volunteers to different blood bags and each blood was separated into 3 groups (control, resveratrol and serotonin groups). Malondialdehyde (MDA), ubiquinone-10 (CoQ10) and 8-hydroxy-2-deoxyguanosine/106deoxyguanosine (8-OHdG/106dG) levels were determined at baseline and on the 7th, 14th, 21st and 28th day after addition of resveratrol and serotonin. MDA, CoQ10 and 8-OHdG/106dG levels increased over time in the control and serotonin groups (p<0.05). However, resveratrol protected against the time-dependent increase in MDA and CoQ10 values (p>0.05). MDA and CoQ10 levels were significantly lower in the resveratrol-treated group on the 21st and 28th days compared with the other groups (p<0.05). These results show that oxidation sensitivity increased time dependently in stored blood. However, the addition of resveratrol allowed the extension of the blood’s shelf life by protecting against increased lipid peroxidation and oxidized CoQ10. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Beta-Fibrinogen 455 G/A and Angiotensin Converting Enzyme Ins/Del Polymorphisms in Patients With Lung Cancer
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) Alp, H.H.; Umudum, F.Z.; Bakan, E.
In our study we have investigated polymorphism due to base change occurring at-455 position of promoter region of β-fibrinogen synthesizing gene in lung cancer patients. In addition, we have also investigated insertion/deletion (I/D) polymorphism in intron 16 of Angiotensin Converting Enzyme (ACE) gene, located on chromosome 17q23. Aim of our study is to determine polymorphisms of β-fibrinogen-455 G/A and ACE I/D in patients with lung cancer and healthy control and to determine whether these polymorphisms contribute to the format ion of lung cancer. In addition, we aimed to clarify whether β-fibrinogen-455 G/A polymorphism effect the level of fibrinogen synthesis in patients with lung cancer. Samples in our study was obtained from 100 patients with lung cancer and 100 healthy volu nteers. Plasma fibrinogen levels were measured with ELISA method. Polymorphism analyses were determined with PCR-reverse hybridization method. Results of our study revealed that fibrinogen levels of patients with lung cancer were significantly higher than healthy control group (p<0.01). No difference was determined for β-fibrinogen-455 G/A and ACE I/D polymorphisms between patients with lung cancer and healthy control group. In both of the groups fibrinogen level was higher in AA genotype compared to GG and GA genotypes in both of the groups (p<0.05) As a result, it can be stated that β-fibrinogen-455 G/A and ACE I/D polymorphisms are not related with formation and prognosis of lung cancer. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Can the Irisin Be a Biomarker for Prostate Cancer? a Case Control Study
(Asian Pacific Organization for Cancer Prevention, 2020) Aslan, R.; Alp, H.H.; Eryılmaz, R.; Huyut, Z.; Sevim, M.; Araz, S.; Taken, K.
Aim: There is much evidence of an association between cancer and irisin that is an adipokine. This study researched on the relationship between prostate cancer (PCa) and irisin levels, and whether irisin can be used as a biomarker in the diagnosis of PCa. Materials and Methods: For the study groups, 50 primary PCa patients and 30 healthy male subjects were included in the PCa and healthy control groups, respectively. All volunteers in the healthy control group were screened for prostate cancer and other malignancies and chronic diseases. Volunteers who were determine to be completely healthy were included for healthy control group. In the serum samples of the subjects were measured free PSA, total PSA and irisin levels. Irisin levels were compared separately in terms of the Gleason scores and T stage. In addition to intergroup comparisons, the ROC curve for the irisin was plotted and power analysis was performed. Results: Free and total PSA levels in the PCa group were significantly higher compared to the healthy control group (p<0.05). In addition, irisin levels in the PCa group were significantly lower than in the healthy control group (p<0.05). There was no significant difference between irisin levels in the groups classified in terms of Gleason scores (p>0.05). When the cut-off value was taken as 8.1, the sensitivity and specificity of irisin for PCa were as 80.5% and 90%, respectively. Conclusion: The results of this study indicate that the levels of irisin in the PCa group are considerably reduced and irisin may be used as a biomarker as well as free and total PSA. © This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International License.
Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat’s Liver
(Yuzuncu Yil Universitesi Tip Fakultesi, 2020) Huyut, Z.; Bakan, N.; Çokluk, E.; Akbay, H.İ.; Alp, H.H.; Şekeroğlu, M.R.
Studies reported that phosphodiesterase-5 inhibitors (PDE-5Is) positively contributed to bone-mineral-density and thickness in rats with ovariectomy, which have the same condition with postmenapozal period. To explain the positive contribution mechanism on bone mineral density of PDE-5Is, we investigated the effect of zaprinast and avanafil on levels of some pro-or anti-resorptive cytokines in ovariectomized-rats. Albino female rats (8 months and 250-350 g) were used and four groups of equal-number were randomly assigned (n=6). Groups; was the sahm operated, positive control (OVX), Zaprinast and OVX, Avanafil and OVX groups, respectively. The levels of Estrogen, IL-1β, IL-6, IL-8, IL-10 and TNF-α were measured by ELISA kits, in liver of rats. IL-1β, IL-6, IL-8 and TNF-α levels were high in groups with OVX compared to sham group, while IL-10 levels were low. Also, IL-1β, IL-6, IL-8 and TNF-α levels were low in zaprinast and especially avanafil-treated groups with OVX and were similar to the sahm group values (p=0.001 for IL-1β, p=0.045 for IL-6, p=0.008 for IL-8, p=0.006 for IL-10, p=0.026 for TNF-α). Zaprinast and especially avanafil inhibited IL-1β, 8 and TNF-α and increased the IL-10 levels compared to the OVX group. This may support opinion that PDE-5Is enhance bone mineralization by inhibiting proresorptive cytokines. © 2020, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
In Stored Human Blood, the Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative Dna Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Huyut, Z.; Şekeroğlu, M.R.; Balahoroğlu, R.; Alp, H.H.; Çokluk, E.
Stored bloods, are exposed to a number of negative changes called as "storage lesions" morphological, biochemical and functional, to begin the first phase of the storage. In this study, we investigated the effects of caffeic and tannic acid in lipid peroxidation and oxidative DNA damage in stored blood. The blood was taken from 10 healthy male individuals to the blood bag containing CPD. Each donor's blood was separated into three groups. The first group was used as the control group with nothing added to the blood. A total of 30 µg/mL caffeic acid and 15 µg/mL tannic acid was added to the second and third group, respectively. Malondialdehyde, ubiquinone-10 and 8-hydroxy-2 deoxyguanosine levels were determined at baseline and on the 7th, 14th, 21st and 28th day, by high pressure liquid chromatography. The MDA and 8-OHdG levels of control and tannic acid groups were increased on the 21st and 28th days (p<0.001), whereas in caffeic acid group was preserved. Also, CoQ10 levels increased in the control group time dependently (p<0.001), but preserved in the other two groups. In addition, CoQ10 and 8-OHdG levels of tannic and especially caffeic acid groups was lower than the control group in the inter-group comparison, in periods of progressing time (p<0.05). These results showed that oxidative stress increased in the stored blood, but adding tannic and caffeic acid into the stored blood can attenuate the effects of oxidation. © 2016, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of the Predictive Value of Amino Acids for Tuberculous Meningitis, Aseptic Meningitis and Bacterial Meningitis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Binici, I.; Huyut, Z.; Alp, H.H.; Akbay, H.I.; Karsen, H.; Parlak, M.; Akmeşe, Ş.
In our study, we aimed to detect amino acid changes, if any, by comparing the levels of amino acids in cerebrospinal fluid (CSF) samples of patients with aseptic, bacterial, and tuberculous meningitis and control groups. Patients diagnosed with aseptic meningitis (n=41), tuberculous meningitis (n=21), bacterial meningitis (n=41) and a control group consisting of 64 individuals with similar gender and age characteristics were included in the study. 2 mL of cerebrospinal fluid specimens were obtained from all patients and control group and sto red at-80 °C until the study day. The amino acid measurements were performed using commercially available liquid chromatography-tandem mass spectrometry method (LC-MS / MS) kits. When we investigated amino acids levels in all groups, the levels of 1-methyl histidine, alanine, asparagine, histidine, isoleucine, lysine, methionine, norvaline, ornithine, phenylalanine, proline, sarcosine, threonine, tyrosine, valine in the aseptic, tuberculosis and bacterial meningitis groups were statistically higher than t hose of the control group. The levels of all amino acids except cystine, glutamic acid, homo-citrulline, and taurine in the patients with aseptic meningitis were statistically significantly higher than in the control group. The levels of all amino acids ex cept homocitrulline, and tryptophan were statistically significantly higher in the patient group with tuberculous meningitis than in the control group. The levels of all amino acids except glutamic acid were statistically significantly higher in the patient group with bacterial meningitis than in the control group. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Levels of Leukocyte Oxidative Dna Damage (8-Ohdg), Serum Coenzyme Q10 and Lipid Peroxidation in the Formation Attacks of Patients With Multiple Sclerosis
(Suleyman Demirel University, 2017) Cokluk, E.; Milanlıoğlu, A.; Huyut, Z.; Çilingir, V.; Alp, H.H.; Aydın, M.N.; Balahoroğlu, R.
Multiple sclerosis (MS) is a demyelinating disease of the nervous system. Evidence about oxidative stress plays an important role in the pathogenesis of MS is increasing day by day. In our study, we aimed to investigate the effect of oxidative DNA damage and oxidative stress in the pathogenesis of MS disease. Blood samples were obtained from during an attack (Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and 30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of oxidized lipids were detected using flourescence dedector with high pressure liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and patients with MS and then we measured 8-hydroxy-2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum MDA level of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). 8-OHdG/106 dG ratio of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared with group 2 and group 3 (p<0.001). In conclusion, we observed that oxidative DNA damage, lipid and mitochondria oxidative damage were high in blood of patients with MS. It seems that oxidative stress acts a play role the pathogenesis of MS patients as well as induces attacks. © 2017 Suleyman Demirel University. All rights reserved.