Browsing by Author "Alp, Hamit Hakan"

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Analysis of Endogenous Oxidative Damage Markers and Association With Pulmonary Involvement Severity in Patients With Sars-Cov Pneumonia
(Elsevier France-editions Scientifiques Medicales Elsevier, 2021) Yildiz, Hanifi; Alp, Hamit Hakan; Ekin, Selami; Arisoy, Ahmet; Gunbatar, Hulya; Asker, Selvi; Haylu, Mine
Introduction: The SARS-CoV-2 virus affects many organs, especially the lungs, with widespread inflammation. We aimed to compare the endogenous oxidative damage markers of coenzyme Q10, nicotinamide dinucleotide oxidase 4, malondialdehyde, and ischemia-modified albumin levels in patients with pneumonia caused by SARS-CoV-2 and in an healthy control group. We also aimed to compare these parameters between patients with severe and non-severe pulmonary involvement. Methods: The study included 58 adult patients with SARS-CoV-2 pneumonia and 30 healthy volunteers. CoQ10 and MDA levels were determined by high-pressure liquid chromatography. NOX4 and IMA levels were determined by ELISA assay and colorimetric method. Results: Higher levels of CoQ10, MDA, NOX4, and IMA and lower levels of COQ10H were observed inpatients with SARS-CoV-2 pneumonia than in the control group. MDA, IMA, NOX4, and CoQ10 levels were significantly higher in patients with severe pulmonary involvement than in patients with non-severe pulmonary involvement, but no significant difference was observed in CoQ10H levels. CoQ10 levels were significantly and positively correlated with both ferritin and CRP levels. Conclusion: SARS-CoV-2 pneumonia is significantly associated with increased endogenous oxidative damage. Oxidative damage seems to be associated with pulmonary involvement severity.
Bazı Fosfodiesteraz 5 İnhibitörlerinin Ovariektomize Sıçanların Karaciğer Dokusunda Oksidatif Stres, Vegf, Bmp 2 ve 9 Üzerine Etkileri
(2023) Alp, Hamit Hakan; Huyut, Zübeyir; Cihan, Murat; Şekeroğlu, Mehmet Ramazan; Alyar, Gülşah; Yildirim, Serkan; Akbay, Halil İbrahim
Amaç: Osteoporoz önemli bir sağlık sorunudur ve henüz etkili bir tedavisi yoktur. Fosfodiesteraz 5 inhibitörleri osteoporoz tedavisi için umut verici ajanlardır. Bu çalışmada, fosfodiesteraz 5 inhibitörlerinin (vardenafil, tadalafil ve udenafil) ovariektomi ile osteoporoz oluşturulan sıçanların karaciğer dokusunda kemik morfojenik protein-2 ve 9 (BMP-2 ve 9), vasküler endotelyal büyüme faktörü (VEGF) ve oksidatif stres belirteçleri (malondialdehit ve CoQ10) üzerindeki etkilerini belirlemeyi amaçladık. Gereç ve Yöntem: 50 Albino wistar dişi sıçan her grupta 10 sıçan olacak şekilde rastgele 5 gruba ayrıldı. Gruplar sırasıyla sham-operated, ovariectomise (OVEX), OVEX + Tadalafil, OVEX + udenafil ve OVEX + vardenafil idi. VEGF, BMP-2 ve 9 seviyeleri ELISA kitleri ile ölçülmüştür. MDA ve CoQ10 seviyelerini tespit etmek için yüksek basınçlı sıvı kromatografi yöntemi kullanılmıştır. Bulgular: PDE-5 inhibitörleri uygulanan gruplarda VEGF, BMP-2 ve 9 seviyeleri sham ve OVEX gruplarına göre anlamlı derecede yüksekti. OVEX+vardenafil ve OVEX+udenafil grupları arasında VEGF, BMP-2 ve 9 düzeyleri açısından anlamlı bir fark bulunmamıştır. PDE5 inhibitörü uygulanan gruplarda MDA ve CoQ10 düzeyleri OVEX grubuna göre anlamlı derecede düşüktü. Histolojik ve immünohistokimyasal sonuçlar incelendiğinde, PDE-5 inhibitörü gruplarında anjiyogenezin anlamlı derecede yüksek olduğu görüldü. Sonuç: Sonuç olarak, bu inhibitörlerin karaciğer dokusunda VEGF, BMP-2 ve 9 ekspresyonunu indükleyerek kemik mineralizasyonu ve yeniden şekillenmesi üzerinde olumlu etkileri olabileceğini söyleyebiliriz.
Blood Prestin Levels in Covid-19 Patients
(Lippincott Williams & Wilkins, 2023) Turan, Mahfuz; Alp, Hamit Hakan; Yildiz, Hanifi; Baran, Ali Irfan; Ekin, Selami; Akin, Ramazan; Bozan, Nazim
Background: Many studies have found that viral infections affect different tissues, including the inner ear. Coronavirus disease 2019 (COVID-19), a viral infection, is a significant health problem worldwide. Prestin is a motor protein with important functions both in the outer hair cells of the inner ear and in cardiac tissue. In addition, prestin is promising as an early biomarker in the detection of ototoxicity. To determine the severity of infection in COVID-19 patients and to determine whether other tissues are affected by the infection, lactate dehydrogenase (LDH), C-reactive protein (CRP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase MB (CK-MB), biochemical markers such as ferritin and D-dimer are used. This study aimed to compare prestin levels in patients with COVID-19 and healthy volunteers.Methods: In blood samples taken from 45 patients diagnosed with COVID-19 and 40 healthy volunteers, prestin levels were determined with the kit that used an enzyme-linked immunosorbent assay method and was commercially available. At the same time, LDH, CRP, ALT, AST, CK-MB, ferritin, and D-dimer levels were also detected in both patients and healthy control groups and correlations with prestin levels were examined.Results: The main result of our study is that serum prestin levels in COVID-19 patients are significantly higher than in healthy controls (p < 0.001). In addition, a statistically significant strong positive correlation was found between prestin-LDL (r = 0.537, p = 0.001), prestin-CRP (r = 0.654, p = 0.001), and prestin-D-dimer (r = 0.659, p = 0.001).Conclusion: The levels of prestin, a motor protein in inner ear outer hair cells and cardiac myocytes, were found to be higher in COVID-19 patients than in healthy volunteers. It also showed a positive correlation with CRP and D-dimer. This may be associated with systemic dysfunction.
Clinical Vs. Statistical Significance: Considerations for Clinical Laboratories
(Walter de Gruyter Gmbh, 2025) Alp, Hamit Hakan; Tran, Mai Thi Chi; Markus, Corey; Ho, Chung Shun; Loh, Tze Ping; Zakaria, Rosita; Greaves, Ronda F.
Amongst the main perspectives when evaluating the results of medical studies are statistical significance (following formal statistical testing) and clinical significance. While statistical significance shows that a factor's observed effect on the study results is unlikely (for a given alpha) to be due to chance, effect size shows that the factor's effect is substantial enough to be clinically useful. The essence of statistical significance is "negative" - that the effect of a factor under study probably did not happen by chance. In contrast, effect size and clinical significance evaluate whether a clinically "positive" effect of a factor is effective and cost-effective. Medical diagnoses and treatments should never be based on the results of a single study. Results from numerous well-designed studies performed in different circumstances are needed, focusing on the magnitude of the effects observed and their relevance to the medical matters being studied rather than on the p-values. This paper discusses statistical inference and its relevance to clinical importance of quantitative testing in clinical laboratories. To achieve this, we first pose questions focusing on fundamental statistical concepts and their relationship to clinical significance. The paper also aims to provide examples of using the methodological approaches of superiority, equivalence, non-inferiority, and inferiority studies in clinical laboratories, which can be used in evidence-based decision-making processes for laboratory professionals.
Comparison of the Effectiveness of Thymoquinone, St. John Wort Oil and Silver Sulfadiazine in Experimental Burn Wounds
(Oxford Univ Press, 2024) Canbaz, Yasin; Karakol, Percin; Erten, Remzi; Mercantepe, Tolga; Alp, Hamit Hakan; Canbaz, Esra Turan; Yuce, Serdar
We aimed to compare the effectiveness of thymoquinone (TQ), the most important bioactive component of black cumin, St. John wort (SJW) oil, a traditional medicinal plant used in burns, and silver sulfadiazine (AgSD), a well-known antiinflammatory agent used in modern medicine, in an experimental burn rat-model. Sixty-three Wistar-Albino rats were randomly divided into 9 groups (n = 7). TQ and SJW were administered topically and systemically but AgSD was applied topically. Epithelialization, inflammatory cell response, granulation tissue, vascularization, and fibrosis were evaluated. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), vitamin E, 8-hydroxy-deoxyguanosine (8-OHdG), coenzyme Q10 (CoQ10) were analyzed in serum. Topical TQ accelerated the epithelialization, enabled granulation, vascularization, and fibrosis in wounds (P = .001). Topical and systemic TQ increased Vitamin E levels (P = .003) but reduced TOS and 8-OHdG levels (P = .001). Topical SJW reduced granulation and vascularization. Topical and systemic SJW decreased TOS, MDA, and 8-OHdG levels (P = .001) but increased TAS (P = .001) and Vitamin E levels (P = .003). Topical AgSD reduced TOS, 8-OHdG, and MDA levels (P = .001). Topical and systemic TQ demonstrated significant advantages in accelerating the wound healing process while also enhancing antioxidant defenses and reducing oxidative damage. SJW oil, particularly in topical application, improved epithelialization, and antioxidant status but showed less efficacy in systemic use. AgSD, while effective in reducing oxidative stress, was less successful in promoting wound healing and appeared to delay granulation and fibrosis. Thymoquinone offers superior protective and healing benefits, SJW is effective locally but less so systemically, and AgSD should be used cautiously, potentially combined with antioxidants to mitigate its negative impact on wound healing.
Comparison of the Protective Effects of Curcumin and Caffeic Acid Phenethyl Ester Against Doxorubicin-Induced Testicular Toxicity
(Wiley, 2021) Huyut, Zubeyir; Alp, Hamit Hakan; Yaman, Turan; Keles, Omer Faruk; Yener, Zabit; Turkan, Fikret; Ayengin, Kemal
Whether testicular toxicity is mediated by matrix metalloproteinases (MMPs) is an important question that has not been examined. This study investigated the suppressive effect of curcumin and caffeic acid phenethyl ester (CAPE) on oxidative stress, apoptosis, and whether MMPs mediate doxorubicin (DOX)-induced testicular injury. Male rats were randomly divided into eight groups (n = 8 per group). The groups were as follows: sham, dimethyl sulphoxide (100 mu L), DOX (3 mg/kg), CAPE (2.68 mg/kg), curcumin (30 mg/kg), DOX+CAPE (3 mg/kg DOX and 2.68 mg/kg CAPE), DOX+curcumin (3 mg/kg DOX and 30 mg/kg curcumin) and DOX+CAPE+curcumin (3 mg/kg DOX, 2.68 mg/kg CAPE and 30 mg/kg curcumin). Injections were administered daily for 21 days. The oxidative stress, MMPs, proinflammatory cytokines and apoptotic markers in the DOX group were higher than the sham group (p < .05); these measures were lower in the groups treated with CAPE and curcumin together with DOX compared with the DOX group (p < .05). The results showed that MMPs mediated DOX-induced testicular injury, but CAPE and especially curcumin suppressed testis injury and cell apoptosis by suppressing DOX-induced increases in MMPs, oxidative stress and proinflammatory cytokines. However, curcumin exhibited more pronounced effects than CAPE in terms of all studied parameters.
A Cross-Sectional Measurement of Endogenous Oxidative Stress Marker Levels in Obsessive Compulsive Disorder
(Aves, 2022) Kurhan, Faruk; Kamis, Gulsum Zuhal; Alp, Hamit Hakan; Cim, Emine Fusun Akyuz; Atli, Abdullah
Background: There is a correlation between the increase in reactive oxygen radicals and the presence of specific mental illnesses. In this context, the objective of this study is to investigate the relationship between obsessive-compulsive disorder and the variations in the levels of several endogenous oxidative stress markers. Methods: Thirty obsessive-compulsive disorder patients were included in the study as the patient group, and 30 healthy volunteers of matching demographic characteristics were included in the study as the control group. Accordingly, the patient group consisted of 10 females and 20 males with a mean age of 29.5 +/- 6.1 years, and the control group consisted of 15 females and 15 males with a mean age of 31.9 +/- 5.6 years. The serum nicotinamide adenine dinucleotide phosphate oxidase-2, nicotinamide adenine dinucleotide phosphate oxidase-4, and malondialdehyde levels of the 2 groups were compared using the independent samples t-test. The relationships between the serum nicotinamide adenine dinucleotide phosphate oxidase-2, nicotinamide adenine dinucleotide phosphate oxidase-4, and malondialdehyde levels of the 2 groups were analyzed using the Pearson's correlation coefficient. Results: The serum nicotinamide adenine dinucleotide phosphate oxidase-2, nicotinamide adenine dinucleotide phosphate oxidase-4, and malondialdehyde levels of the patient group were statistically significantly higher than those of the control group (P < .001). Statistically significant positive correlations were detected between the serum nicotinamide adenine dinucleotide phosphate oxidase-2 and nicotinamide adenine dinucleotide phosphate oxidase-4 levels (r=0.692, P= .001) and between the serum nicotinamide adenine dinucleotide phosphate oxidase-2 and malondialdehyde levels (r=0.563, P. .001). Conclusion: The results of this study indicated that oxidative stress and lipid peroxidation levels were higher in obsessive-compulsive disorder patients. Based on this finding, NOX-2 and NOX-4 levels can be used as indicators of endogenous oxidative stress in obsessive-compulsive disorder patients.
Determination of Endothelial Nitric Oxide Synthase Gene Polymorphism and Plasma Asymmetric Dimethyl Arginine Concentrations in Patients With Lung Cancer
(Aves, 2020) Bayraktutan, Zafer; Kiziltunc, Ahmet; Bakan, Ebubekir; Alp, Hamit Hakan
Objective: Genetic factors can contribute to both the occurrence and development of lung cancer. This study aimed to investigate endothelial nitric oxide synthase (eNOS) G894T and T-786C polymorphisms and plasma asymmetric dimethylarginine (ADMA) levels of lung cancer patients in comparison with healthy subjects. Materials and Methods: A total of 200 subjects, 100 patients with lung cancer and 100 healthy volunteers were included in this study. To determine eNOS gene polymorphisms, we collected and analyzed blood samples with polymerase chain reaction (PCR). Plasma ADMA levels were evaluated by high-performance liquid chromatography (HPLC). Results: The difference in gene polymorphisms between lung cancer patients and healthy controls were insignificant. However, lung cancer patients had statistically significantly higher plasma ADMA levels than healthy controls. The patients and control groups with CC polymorphisms and TT polymorphisms on eNOS T-786C and G894T gene regions had higher plasma ADMA levels. The CC polymorphisms and plasma ADMA levels were higher in patients with small-cell lung cancer compared to those in patients with non-small-cell lung cancer. Conclusion: Although eNOS gene polymorphisms had no significant difference between lung cancer patients and healthy controls, plasma ADMA levels were higher in lung cancer patients compared to healthy controls. Our study suggests that CC genotypes and elevated plasma ADMA levels might be associated with small-cell lung cancer.
Determination of Serum Differential Carnitine Ester Levels in Hiv(+)patients: a Cross-Sectional Study
(Bentham Science Publ Ltd, 2023) Binici, Irfan; Akbay, Halil Ibrahim; Huyut, Zubeyir; Alp, Hamit Hakan; Karsen, Hasan; Koyuncu, Ismail; Celik, Hakim
Objective It has been reported that carnitine deficiency is observed in various viral infections and in the follow-up of the prognosis of some diseases. In this cross-sectional study, we aimed to determine how carnitine ester derivatives change in HIV-positive patients. Materials and Methods In this study, 25 HIV-infected patients who applied to Harran University Faculty of Medicine Education Research and Practice Hospital Infectious Diseases and Clinical Microbiology Outpatient Clinic and who did not receive any antiretroviral treatment, as well as 25 healthy volunteers were included in the study. Carnitine ester levels in serum samples were measured by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) method (Shimadzu North America, Columbia, MD, USA). Results While suberoylcarnitine (C8DC), myristoleylcarnitine (C14:1), tetradecadienoylcarnitine (C14:2), palmitoleylcarnitine (C16:1), and linoleylcarnitine (C18:2) levels in HIV(+) patients were quite low compared to the control group, tiglylcarnitine (C5:1) levels were high (p <= 0.05). In addition, C5:1 and C14:2 index parameters according to VIP score, and C5:1 and C14:1/C16 index parameters according to ROC analysis were determined as markers with high potential to distinguish HIV(+) patients from healthy volunteers. Conclusion This study showed that levels of acylcarnitine derivatives might be altered in HIV(+) patients, and the results obtained may contribute to a better understanding of carnitine metabolism.
Dynamic Thiol/Disulfide Homeostasis and Oxidative Dna Damage in Adult Attention Deficit Hyperactivity Disorder
(Korean Coll Neuropsychopharmacology, 2021) Kurhan, Faruk; Alp, Hamit Hakan
Objective: Insufficient number of oxidative stress studies have been conducted in patients with adult attention deficit hyperactivity disorder (ADHD). The objective of the current study is to examine the thiol/disulfide homeostasis as well as oxidative DNA damage levels in adult ADHD patients and to compare them with the results of healthy control subjects. Methods: The study was inclusive of forty-nine patients who were diagnosed with adult ADHD, as well as thirty-three healthy volunteers to be used as the control group. The diagnosis of the patients was conducted according to the DSM-5 diagnostic criteria. Blood were stored under appropriate laboratory conditions. For the purpose of detecting the oxidative DNA damage level, an extraction of genomic DNA from leukocytes was carried out, and furthermore the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), apart from deoxyguanosine, were measured accordingly. Results: Total thiol and the native thiol levels were observed to be statistically lower in adult ADHD patients as compared to the subjects in the healthy control group (p = 0.001). It was observed that the disulfide levels were higher in adult ADHD patients as compared to the healthy control subjects (p = 0.001). In addition, the levels of 8-OHdG, which are considered as a marker for assessing DNA damage, were found to be significantly lower in the control group as compared to the adult ADHD patients (p = 0.001). Conclusion: It was observed that the thiol/disulfide homeostasis had shifted towards disulfide, and 8-OHdG levels were increased in adult ADHD patients.
Effect of Dynamic and Static Strength Training on Hormonal Activity in Elite Boxers
(Gdansk Univ Physical Education & Sport, 2020) Aggon, Eser; Agirbas, Ozturk; Alp, Hamit Hakan; Ucan, Izzet; Gursoy, Recep; Hackney, Anthony C.
Background: The aim of this study was to determine hormonal responses to acute and chronic exposure to static and dynamic strength training programs using resistance bands in boxers. Material and methods: 19 male national boxers participated in the study. Boxers were instructed to perform strength exercises with resistance bands for 3 days a week for 8 weeks involving either dynamic (n=10) or static (n=9) resistance exercises. Blood samples were taken before exercise, immediately after the initial exercise session, and 8 weeks later following the last exercise session. Cortisol, growth hormone, adrenocorticotropic hormone adrenaline and noradrenaline levels were measured. Statistical analyses involved non-parametric analysis with an alpha level of .05. Results: Dynamic strength exercises were effective stimuli to growth hormone, adrenaline and noradrenaline, while static strength exercises provoked cortisol, growth hormone, adrenaline and noradrenaline responses both initially after exercise and after 8 weeks of chronic training. Neither dynamic nor static strength exercises were effective in prompting adrenocorticotropic changes after an exercise session or after 8 weeks of training. Conclusions: We showed that dynamic and static strength exercise protocols using resistance bands both could provoke acute and chronic hormonal responses in boxers similar to more traditional modes of such exercise.
The Effect of Etoricoxib on Hepatic Ischemia-Reperfusion Injury in Rats
(Hindawi Ltd, 2015) Kunak, Celalettin Semih; Kukula, Osman; Mutlu, Emre; Genc, Fatma; Peker, Gulcer Gulec; Kuyrukluyildiz, Ufuk; Alp, Hamit Hakan
Ischemia-reperfusion (I/R) damage is known to be a pathological process which continues with the increase of oxidants and expands with the inflammatory response. There is not any study about protective effect of etoricoxib on the liver I/R damage in literature. Objective. This study investigates the effect of etoricoxib on oxidative stress induced by I/R of the rat liver. Material and Methods. Experimental animals were divided into four groups as liver I/R control (LIRC), 50 mg/kg etoricoxib + liver I/R (ETO-50), 100 mg/kg etoricoxib + liver I/R (ETO-100), and healthy group (HG). ETO-50 and ETO-100 groups were administered etoricoxib, while LIRC and HG groups were orally given distilled water by gavage. Hepatic artery was clamped for one hour to provide ischemia, and then reperfusion was provided for 6 hours. Oxidant, antioxidant, and COX-2 gene expressions were studied in the liver tissues. ALT and AST were measured. Results. Etoricoxib in 50 and 100 mg/kg doses changed the levels of oxidant/antioxidant parameters such as MDA, MPO, tGSH, GSHRd, GST, SOD, NO, and 8-OH/Gua in favour of antioxidants. Furthermore, etoricoxib prevented increase of COX-2 gene expression and ALT and AST levels. This important protective effect of etoricoxib on the rat liver I/R can be tested in the clinical setting.
The Effect of Laparoscopic and Open Surgery on Oxidative Dna Damage and Il-37 in Children With Acute Appendicitis
(Springer London Ltd, 2021) Ayengin, Kemal; Alp, Hamit Hakan; Avci, Veli; Huyut, Zubeyir
Background Although laparoscopic appendectomy (LA) is considered to be minimally invasive compared with open appendectomy (OA), there are few studies showing this in terms of cytokines and oxidative stress markers. Aim LA and OA techniques have been compared in terms of different conditions such as operation time and complication rate. Here, we compared how oxidative DNA damage and some cytokines levels change in relation to LA and OA. Method This study was performed in children diagnosed with acute appendicitis in our hospital. The study was conducted on 30 children with acute appendicitis in each group. Oxidative DNA damage and malondialdehyde levels were determined by high-performance liquid chromatography, and interleukin-1 beta, interleukin-37, ischaemia-modified albumin and total thiol levels were determined by enzyme-linked immunosorbent assays. Results There was a dramatic decrease in ischaemia-modified albumin (IMA) levels after LA. However, there were no statistically significant differences in pre- and postoperative IMA levels in the OA group. Oxidative DNA damage and malondialdehyde levels were low significantly according to preoperative levels after the LA and OA. Total thiol levels were high in children who underwent LA while they were low after OA. In addition, postoperative interleukin-1 beta levels were low in both groups. Furthermore, IL-37 levels postoperatively were low in the LA group, while there was no significant change in the OA group. Conclusion LA had a more positive effect on oxidative DNA damage, IL-1 beta and IL-37 than the OA. In addition, surgical stress was reduced with LA.
The Effect of Metamizole on Ischemia/Reperfusion Injury in the Rat Ovary: an Analysis of Biochemistry, Molecular Gene Expression, and Histopathology
(Medknow Publications & Media Pvt Ltd, 2016) Kumbasar, Serkan; Salman, Suleyman; Al, Ragip Atakan; Ozturk, Cengiz; Yarali, Oguzhan; Alp, Hamit Hakan; Suleyman, Bahadir
Objectives: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats. Materials and Methods: Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion 100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion 200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG). Results: Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells. Conclusions: At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment.
The Effect of Progresive Relaxation Training on Free Radicals, Antioxidant System and Oxydative Dna Damage
(Parlar Scientific Publications (p S P), 2018) Aggon, Eser; Ozturk, Nurinnisa; Alp, Hamit Hakan; Siktar, Elif; Agirbas, Ozturk; Bakan, Ebubekir
This study aimed in sedentary subjected to progressive relaxation training (PRT) for determination of the effect of this exercise on MDA, OHdG, and NO levels and SOD and GPx activities. Experimental design was used in this research. Seven healthy, sedentary volunteers were included in the study. All subjects were male and within 15 to 18 years of age. Jacabson's Progressive Muscle Relaxation Training was applied to the volunteers for 6 six weeks. Before and after the chronic and acute exercise, blood samples were taken from the subjects, and K-Related Samples Friedman test was used for the analysis of repeated measurements and for the comparison of pre- and post-exercise data and chronic and acute mode data. In addition, 2-Related Samples Wilcoxon test was used in the evaluation of the groups as pairs. Our findings showed increases in MDA PRT, and no statistically significant increase in NO levels after PRT in sedentary volunteers. When the effect of relaxation exercise on the antioxidant system is considered, both SOD and GPx activities increased. The effect of PRT on 8-OHdG has been found to exhibit significant increase after PRT. It can easily be said that PRT may be helpful in patients with chronic disease, especially for obtaining a healing and a high quality sleep. In addition, in order for the athletes to feel well and to use their muscles actively, PRT may be helpful. We can conclude that, with respect to human health and physiology, no controversy may be seen in progressive muscle relaxation training.
The Effect of Thiamine Pyrophosphate on Ethambutol-Induced Ocular Toxicity
(Taylor & Francis Ltd, 2016) Cinici, Emine; Cetin, Nihal; Ahiskali, Ibrahim; Suleyman, Bahadir; Altuner, Durdu; Alp, Hamit Hakan; Suleyman, Halis
Context: Ethambutol-induced retinal oxidative damage in patients with tuberculosis is still not being adequately treated. The protective effect of thiamine pyrophosphate against oxidative damage in some tissues has been reported, but no information on the protective effects of thiamine pyrophosphate against ethambutol-induced oxidative retinal damage has been found in the medical literature.Objective: The objective is to investigate whether thiamine pyrophosphate has a protective effect against oxidative retinal damage in rats induced by ethambutol.Materials and methods: Experimental animals divided into four groups (n=10): the healthy group (HG), the ethambutol control group (EMB), thiamine+ethambutol group (Thi-EMB) and thiamine pyrophosphate+ethambutol group (TPP-EMB). The rats in the TPP-EMB and Thi-EMB groups were administered thiamine pyrophosphate and thiamine, respectively, at doses of 20mg/kg intraperitoneally. Distilled water was administered intraperitoneally to the HG and the EMB groups as a solvent in the same volumes. One hour after drug injection, 30mg/kg ethambutol was administered via an oral gavage to the TPP-EMB, Thi-EMB and EMB groups. This procedure was repeated once a day for 90 days. At the end of this period, all rats were euthanized under high-dose thiopental sodium anesthesia, and biochemical and histopathological investigations of the retinal tissue were performed.Results: Malondialdehyde (MDA) and DNA damage product 8-hydroxyguanine levels were significantly lower in the retinal tissue of TPP-EMB and HG groups compared to those of the Thi-EMB and EMB groups, and total glutathione (tGSH) was also found to be higher. In addition, severe retinal tissue vascularization, edema and loss of ganglion cells were observed in the Thi-EMB and EMB groups, whereas histopathological findings for the TPP-EMB group were observed to be close to normal.Discussion and conclusion: These findings suggest that thiamine pyrophosphate protects retinal tissues from ethambutol-induced oxidative damage, and thiamine does not. This positive effect of thiamine pyrophosphate may be useful in the prevention of ocular toxicity that occurs during ethambutol use.
Effect of Thymoquinone on the Healing of Left Colon Anastomosis: an Experimental Study
(Springer international Publishing Ag, 2016) Kiziltan, Remzi; Yilmaz, Ozkan; Celik, Sebahattin; Yildirm, Serkan; Alp, Hamit Hakan; Aras, Abbas; Kotan, Cetin
Aim: To evaluate the effect of thymoquinone on the healing of experimental left colon anastomosis in rats. Methods: Forty Wistar albino rats weighing 250-300 g were randomly divided into four groups (10 rats/group). Group 1 (control group) rats were not administered Thymoquinone (TQ) for 3 days after the operation. Group 2 was administered daily TQ for 3 days starting from the first day after the operation. Group 3 was not administered TQ for 7 days after the operation. Group 4 was administered daily TQ for 7 days starting from the first day after the operation. Thymoquinone was administered as a single dose oral gavage through a 4F feeding catheter per each day. The bursting strength of the anastomosis was measured on 3rd and 7th postoperative days (POD) and resection was performed. Subsequently, the hydroxyproline level in the resected tissue was measured and a histological evaluation was performed. Results: The bursting pressures of the anastomoses were measured to be statistically significantly greater on 7th POD in TQ administered groups compared to those without TQ administration. Tissues were stained with Masson's trichrome dye in order to evaluate the amount of fibrous tissue reaction for histopathological examination; there was no significant difference in the amount of fibrous tissue between groups 1 and 2, while a very marked increase in the fibrous tissue was detected in groups 3 and 4. Mean tissue hydroxyproline levels of the groups 3 and 4 on 7th POD were 1.30 and 2.72 mu g/g-protein, respectively. The difference between the groups was statistically significant (p = 0.001). Conclusions: TQ significantly increased the bursting pressure of the anastomosis, tissue hydroxyproline level, and fibrous tissue production.
The Effect of Vitamin E Prophylaxis on Experimental Amikacin-Induced Nephrotoxicity Model
(Oxford Univ Press, 2016) Bulut, Gulay; Ari, Elif; Basbugan, Yildiray; Alp, Hamit Hakan; Bayram, Irfan
Effects of Composite Restorations on Oxidative Stress in Saliva: an in Vivo Study
(Elsevier Taiwan, 2015) Gul, Pinar; Akgul, Nilgun; Alp, Hamit Hakan; Kiziltunc, Ahmet
Background/purpose: This study is aimed at detecting and quantifying monomers from dental composite filling materials and investigating effects of the released monomers in saliva malondialdehyde (MDA) levels and in two antioxidant enzyme levels after dental composite filling materials were applied to healthy volunteers. Materials and methods: Fifty-two individuals (32 female and 20 male) participated in the research study. Saliva samples were collected in scheduled time intervals and MDA, superoxide dismutase, and glutathione peroxidase levels were measured. In addition, saliva samples were collected in order to quantify triethyleneglycol dimethacrylate, hydroxyethyl methacrylate, bisphenol-A diglycidyl methacrylate, and urethane dimethacrylate using high-performance liquid chromatography. Statistical analysis of obtained data was performed by repeated measures variance analysis. A P value <0.05 was considered as statistically significant. Results: As a result of the statistical analysis, the MDA and glutathione peroxidase levels measured at all time intervals were significantly higher when compared to the baseline levels (P < 0.05). Superoxide dismutase values measured after 7 days showed a statistically significant decrease (P < 0.001). The residual monomer levels showed a significant increase within 7 days, and the maximum amount of release was observed generally in the 7-day period, and the release in monomers except for bisphenol-A diglycidyl methacrylate showed a significant decrease in 30 days (P < 0.05). Conclusion: Because of monomer release after the restoration process and also differences in antioxidant enzyme activity, composite materials may lead to oxidative stress. Copyright (C) 2014, Association for Dental Sciences of the Republic of China. Published by Else-vier Taiwan LLC. All rights reserved.
The Effects of Coq10 Supplement on Matrix Metalloproteinases, Oxidative Dna Damage and Pro-Inflammatory Cytokines in Testicular Ischaemia/Reperfusion Injury in Rats
(Wiley, 2021) Ayengin, Kemal; Alp, Hamit Hakan; Huyut, Zubeyir; Yildirim, Serkan; Altindag, Fikret; Avci, Veli
We aimed to study the effect of coenzyme Q10 on pro-inflammatory cytokine, matrix metalloproteinase, oxidative DNA damage, caspase 3 and caspase 8 in ischaemia/reperfusion injury led to by testicular torsion/detorsion. Our research is a controlled experimental animal research using rats. This study was conducted with fifty-six adult male Albino Wistar rats. Interleucine-1 beta, 2, 6, 10, tumour necrosis factor-alpha, matrix metalloproteinase-2, 3, 9, 13, tissue inhibitor matrix metalloproteinase-1, 2, malondialdehyde and leucocyte 8-hydroxy-2-deoxy guanosine/10(6) deoxyguanosine was detected in serum and tissue samples. In addition, immunohistochemical analysis of caspase 2 and caspase 8 was performed. In testicular I/R injury, especially 24 hr after detorsion, oxidative damage pro-inflammatory cytokines and matrix metalloproteinases were increased. At the coenzyme Q10 group, a meaningful decrease was observed in these parameters. In addition, a decrease in the expression of caspase3 and caspase 8 was viewed in coenzyme Q10-treated groups. The coenzyme Q10 has beneficial effects on oxidative damage, pro-inflammatory cytokine levels, remodelling of extracellular matrix and apoptosis in testicular I/R injury.