Browsing by Author "Alparslan, Bayram"

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Bee Venom and Melittin: Potent Key Enzyme Inhibitors With Promising Therapeutic Potential
(Pergamon-elsevier Science Ltd, 2024) Alparslan, Bayram; Senturk, Murat; Erkan, Cengiz
Bee venom (BV) is a versatile product with extensive applications, boasting antibacterial and anticancer properties. Within this study, we focused on isolating melittin (Mel) from Apis mellifera L. venom and exploring the influence of both BV and Mel on specific enzymes, namely carbonic anhydrase (CA) I, CA II, CA IX, glutathione reductase (GR), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and neuraminidase (NA). The rationale for selecting these enzymes is that their inhibitors have a particular interest in medicinal chemistry in the treatment of several diseases. BV was obtained using a poison collection apparatus, and Mel was isolated by means of High-Performance Liquid Chromatography (HPLC). All enzymes, except for CA I and CA II, were commercially sourced and of high purity, and the enzyme assays were carried out spectrophotometrically. Our findings showed that BV inhibited the enzymes with IC50 values of 0.583-3.32 ng/mL, and Mel showed an inhibition range of 0.528-3.2 ng/mL. These results underscore the potential therapeutic promise of BV and Mel as robust enzyme inhibitors, offering prospects for addressing diverse health conditions.
Effective Control of Varroa Destructor Mites With Juniperus Excelsa, Juglans Regia, Origanum Onites, and Mentha Spicata Leaf Extracts: a Study of Honey Bee Colonies
(Taylor & Francis Ltd, 2024) Alparslan, Bayram; Tasli, Hueseyin; Erkan, Cengiz
This study aimed to assess the efficacy of plant leaf extracts from Juniperus excelsa (J. excelsa), Juglans regia (J. regia), Origanum onites (O. onites), and Mentha spicata (M. spicata) in controlling Varroa destructor (V. destructor) mites, a major cause of honey bee colony loss. This research seeks to identify eco-friendly and sustainable alternatives for pest control in beekeeping practices. Forty honey bee colonies naturally infested with mites were selected. The colonies were divided into five groups, one control group and four treatment groups, which received varying concentrations of the plant leaf extracts. This study was conducted in two phases: determining the optimal dosage and conducting a field study during autumn and spring, during which the mite infestation levels were evaluated using the powdered sugar method. The powdered sugar method revealed that a 25% concentration of plant leaf extract was the most effective. In the autumn treatment, the efficacy rates were 87% for J. regia, 86% for J. excelsa, 83% for O. onites, and 68% for M. spicata. During spring treatment, rates were slightly reduced but remained promising, with 66% for O. onites, 63% for J. regia, 61% for J. excelsa, and 55% for M. spicata. This study highlights that plant leaf extracts offer a viable and eco-friendly alternative to traditional therapies for controlling Varroa in beekeeping when used at the appropriate dosage. This research provides valuable insights into sustainable pest management and the potential for innovative, environmentally friendly pest control solutions based on aromatic plant products.
Investigation of the Inhibitory Effect of Bee Venom on Cholinesterase Enzymes
(Wiley, 2022) Senturk, Murat; Erkan, Cengiz; Alparslan, Bayram