Browsing by Author "Alpaslan, N."

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Comparison of Ccl28, Interleukin-8, Interleukin-1β and Tumor Necrosis Factor-Alpha in Subjects With Gingivitis, Chronic Periodontitis and Generalized Aggressive Periodontitis
(Wiley-blackwell, 2013) Ertugrul, A. S.; Sahin, H.; Dikilitas, A.; Alpaslan, N.; Bozoglan, A.
Background and Objective: Cytokines produced by various cells are strong local mediators of inflammation. Mucosa-associated epithelial chemokine (CCL28), interleukin-8 (IL-8), interleukin-1beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-a) are major cytokines that play important roles in the periodontal inflammatory process. In this study we aimed to compare the levels of CCL28, IL-8, IL-1 beta and TNF-a in the gingival crevicular fluid of both periodontally healthy subjects and in subjects diagnosed with gingivitis, chronic periodontitis and generalized aggressive periodontitis. Material and Methods: A total of 84 subjects participated in the study: 21 subjects had gingivitis, 21 subjects had chronic periodontitis, 21 subjects had generalized aggressive periodontitis and 21 were periodontally healthy. The levels of CCL28, IL-8, IL-1 beta and TNF-a were analyzed using enzyme-linked immune sorbent assay (ELISA). Results: The total levels of CCL28 and IL-8 in the gingival crevicular fluid of the generalized aggressive periodontitis group (324.74 +/- 42.62 pg/30 s, 487.62 +/- 49.21 pg/30 s) were significantly higher than those of the chronic periodontitis group (268.81 +/- 28.64 pg/30 s, 423.65 +/- 35.24 pg/30 s), the gingivitis group (146.35 +/- 17.46 pg/30 s, 310.24 +/- 48.20 pg/30 s) and the periodontally healthy group (92.46 +/- 22.04 pg/30 s, 148.41 +/- 24.64 pg/30 s). Similarly, the total levels of IL-1 beta and TNF-a in the generalized aggressive periodontitis group (110.23 +/- 9.20 pg/30 s, 1284.46 +/- 86.32 pg/30 s) were significantly higher than those in the chronic periodontitis group (423.65 +/- 35.24 pg/30 s, 82.64 +/- 9.12 pg/30 s), the gingivitis group (52.10 +/- 7.15 pg/30 s, 824.24 +/- 44.68 pg/30 s) and the periodontally healthy group (36.44 +/- 8.86 pg/30 s, 628.26 +/- 34.61 pg/30 s). Conclusion: CCL28, IL-8, IL-1 beta and TNF-a may play key roles in the host response to inflammation in periodontal diseases. As the severity of periodontal diseases increases, destruction of periodontal tissues also increases. Inflammation is one among many factors that trigger periodontal tissue destruction. Identification of the mediators that influence the development and progression of inflammation in periodontal diseases may be very important in understanding the prognoses of periodontal diseases.
Evaluation of Beta-2 Microglobulin and Alpha-2 Macroglobulin Levels in Patients With Different Periodontal Diseases
(Wiley-blackwell, 2013) Ertugrul, A. S.; Sahin, H.; Dikilitas, A.; Alpaslan, N.; Bozoglan, A.
Background Beta-2 microglobulin (B2M) and alpha-2 macroglobulin (A2M) play key roles in the immune system. The aim of this study was to compare B2M and A2M levels in patients with different periodontal diseases. Methods Eighty patients (20 periodontally healthy, 20 with gingivitis, 20 with chronic periodontitis and 20 with generalized aggressive periodontitis) were enrolled in the study. The analysis of B2M and A2M was performed on gingival crevicular fluid (GCF) using an enzyme-linked immunosorbent assay in GCF. Results The total levels of B2M and A2M were statistically lower in the periodontally healthy group than in the other groups (p<0.05) and significantly higher in the generalized aggressive periodontitis group compared to the other groups (p<0.05). Conclusions B2M and A2M play key roles in the balance between periodontal health and disease. It is proposed that tissues release B2M and A2M to stop inflammation and inhibit the proliferation of microorganisms and this may be the reason for the high levels of B2M and A2M in the generalized aggressive periodontitis and chronic periodontitis groups. B2M and A2M are assumed to be user-friendly and cost-effective markers for periodontal disease to identify asymptomatic diseases.
Gingival Crevicular Fluid Levels of Human Beta-Defensins 1 and 3 in Subjects With Periodontitis And/Or Type 2 Diabetes Mellitus: a Cross-Sectional Study
(Wiley, 2013) Ertugrul, A. S.; Dikilitas, A.; Sahin, H.; Alpaslan, N.; Bozoglan, A.; Tekin, Y.
Background and Objective Human -defensins (hBDs) have a strong antibacterial action against various microorganisms, especially periodontal pathogens. The aim of this study was to compare the total levels of hBD-1 and hBD-3 in the gingival crevicular fluid of healthy patients with gingivitis (HG), healthy patients with chronic periodontitis (HP), patients with type 2 diabetes mellitus (DM) and gingivitis (DM2G) and patients with type 2 DM and chronic periodontitis (DM2P). Material and Methods A total of 80 patients were included: 20 HG, 20 HP, 20 DM2G and 20 DM2P. The levels of hBD-1 and hBD-3 in gingival crevicular fluid were measured using ELISA. Results The DM2P group had significantly higher periodontal clinical parameters at sites from which gingival crevicular fluid was collected compared with the other groups. The HG group had significantly lower periodontal clinical parameters within the gingival crevicular fluid-collected sites than did the HP, DM2G and DM2P groups. The gingival crevicular fluid of the DM2P group had significantly higher levels of total hBD-1 and hBD-3 than did that of the other groups; the hBD-1 and hBD-3 levels were significantly higher in the gingival crevicular fluid of the DM2G group than in that of the the non-DM type 2 groups (HG and HP). The gingival crevicular fluid of the HP group had significantly higher levels of total hBD-1 and hBD-3 in comparison with that of the HG group. Conclusion As a result of the observed vascular and cell activity changes that occur within patients diagnosed with DM, periodontal diseases become more severe. These changes hinder the migration and the ability of chemotactic factors and leukocytes to protect periodontal tissues from the effects of microorganisms. In order to eliminate microorganisms, the epithelial cells in patients with DM may release more hBD-1 and hBD-3 into the gingival crevicular fluid. Determining the amount of hBD-1 and hBD-3 in the gingival crevicular fluid of patients with and without DM will help to elucidate the relationship among hBD-1, hBD-3, DM and periodontal disease.