Browsing by Author "Altintas, Serdar"

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Antioxidant Capacity and Phylogenetic Analysis of Twenty Native Grape Cultivars in Siirt Province, Turkey
(C M B Assoc, 2018) Fidan, Mehmet; Erez, Mehmet Emre; Inal, Behcet; Pinar, Suleyman Mesut; Altintas, Serdar
The quality of grape cultivars not only depends on the grape cultrvar but also is influenced by the molecular concepts and agro-climatic factors. For this purpose, four different grape cultivars were collected from five different locations in Siirt province (Turkey). Totally twenty different grape cultivars were investigated. In the present study, the antioxidant activity (total phenolic, flavonoid, proanthocyanidin content, DPPH and FRAP activity) in seeds were indicated and phylogenetic analysis (cpDNA;trnL-F region) of twenty native grape cultivars were imestigated to construct their phylogenetic tree. According to reported data on antioxidant activity and content of phytochemicals, all cultivars exhibited different values from each other, but Rutik and Gad?v cultivars were found as significantly higher in comparison to others According to bioinformatics analysis, twenty grape cultivars were distributed into six different major groups. Rutik and Sevkeye cultivars exhibit significant distinction from other grape cultivars. The phylogenetic analysis was also associated and supported with the results of obtained data from bioactivity. The bioactivity and phylogenetic analysis were firstly identified and quantified in these grape cultivars, however, with regard to obtained data from the current study, the grape cultivars grown in Siirt province were indicated significant and valuable results and as a result, these cultivars have to be evaluated before extinction.
Evaluation of Mterf Gene Expressions in Response To Phytophthora Capsici Infection in Pepper (Capsicum Annuum L.) Cultivars
(Academic Press Ltd- Elsevier Science Ltd, 2024) Altintas, Serdar; Kuru, Ibrahim Selcuk; Erez, Mehmet Emre; Catkin, Sedriye; Inal, Behcet; Bingol, Omer
Despite resistance to abiotic stresses, some plant species may still show low resistance to pathogens. This biotic stress resistance can provide advantage and increase productivity in agricultural systems. However, while there is extensive research on the pathways involved in abiotic stress tolerance, the impact on the molecular mechanisms underlying pathogen resistance remains poorly understood. Among the pathogens , Phytophthora capsici , an oomycete, causes severe damage as plant pathogen, leading to blight and fruit rot in pepper and other commercially valuable crops. This study investigates the effects of Phytophthora capsici infection on highly resistant (CM334) and susceptible (Sera Demre) pepper varieties. We examined biochemical changes and gene expression across different vegetative periods (seedling, flowering, and fruiting stages). Specifically, we analyzed biochemical pathways and the mitochondrial transcription termination factor (mTERF) gene regions associated with pathogenicity. In pepper seedlings infected with P. capsici , peroxide levels increased at all developmental stages compared to control groups. Additionally, we observed a rise in H 2 O 2 content, a molecule involved in signal transduction, likely due to the oxidative stress caused by the pathogen. The expression patterns of mTERF genes varied significantly depending on both pathogen application and the developmental stage of the plants. In the seedling stage, inoculation with P. capcici resulted in decreased expression of TERF2, mTERF6, and mTERF8 genes in leaf parts of both resistant and susceptible varieties. However, mTERF28 expression showed a significant increase. Interestingly, mTERF14 and mTERF27 gene expressions increased in the resistant variety but decreased in the susceptible one. These findings, although not providing a complete picture of host resistance mechanisms, highlight the crucial role of specific plant genes in the defense pathways against Phytophthora capsici . This knowledge can contribute to selecting pepper varieties with sustainable resistance to this devastating pathogen.
Genetic Diversity Among Some Asparagus Species Using Rdna Its, Cpdna Trnl Intron Sequence and Screening for Antioxidant Activity
(Hard, 2019) Altintas, Serdar; Pakyurek, Mine; Sensoy, Suat; Erez, M. Emre; Inal, Behcet
Three species of genus Asparagus native to Lake Van Basin of Turkey were analyzed using internal transcribed spacer (ITS) and cpDNA trnL intron sequence. Furthermore, these species were screened for their antioxidant activity and total phenolic and flavonoid contents. Phylogenetic analysis based on ITS data revealed two clades: the first clade consisted of Asparagus persicus and Asparagus officinalis, and clade II consisted of Asparagus palaestinus. Similarly, phylogenetic analysis using trnL intron sequence revealed two clades. In term of total phenolic content and total flavonoid content, the highest value were measured for acetone extract of Asparagus persicus sample No. 2 and Asparagus persicus sample No. 6, respectively. The highest antioxidant activity using FRAP assay was found for Asparagus persicus sample No. 3. We concluded that these species could be useful for increasing genetic diversity among cultivated species and for breeding.
Identification of S-Allele Based Self-Incompatibility of Turkish Pear Gene Resources
(Galenos Publ House, 2022) Karatas, Merve Dilek; Hazrati, Nahid; Oguz, Ezgi; Ozmen, Canan Yuksel; Altintas, Serdar; Akcay, M. Emin; Ergul, Ali
Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes (alleles). Pear (Pyrus communis L.) belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Ataturk Horticultural Central Research Institute-Yalova collection is considered as genes bank. Identification of the different features of this collection (fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.) is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR (Polymerase Chain Reaction)-based amplification of the S-allele regions (S-1, S-6, S-7, S-8) causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S-6 allele was the most prevalent one with 63% frequency, while the S-8 allele was the least common allele with a rate of 4%. In allele combinations, the SI-So allele combination was the most common allele combination with a rate of 18%. and trilateral allele combinations (S-1-S-6-S-7 and S-1-S-6-S-8) were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.
Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources Using Allele-Specific Pcr
(Galenos Publ House, 2023) Karatas, Merve Dilek; Hazrati, Nahid; Yuksel Ozmen, Canan; Hasanzadeh, Mohammad; Altintas, Serdar; Akcay, Mehmet Emin; Ergul, Ali
Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Huryemez, Sah elmasi, Tokat, Demir elmasi etc.) obtained from the National Collection of Ataturk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as 'Partially Incompatible' and 12 genotypes involving 4 S-alleles were assigned 'Totally Incompatible'. No S-alleles were observed in 2 genotypes (Pancarlik and Huryemez) which exhibited 'Compatibility' status.