Browsing by Author "Altug, Nuri"

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Atropine Sulphate Test Can Be an Aetiologic Indicator of Vagal Bradycardia Developed in a Megaoesophagus Case
(Natl veterinary Research inst, 2007) Keles, Ihsan; Altug, Nuri; Kaya, Abdullah; Ozkan, Cumali; Yuksek, Nazmi; Aslan, Logman
An acquired megaoesophagus in a calf has been described. The diagnosis of megaoesophagus was made based on clinical and laboratory examinations, using contrast radiography, and necropsy findings. Atropine sulphate was used to enlighten the relationship between damage in the N. vagus and bradycardia, which developed in the megaoesophagus case.
Changes in Serum Adenosine Deaminase and Isoenzyme Levels in Addition To Routine Liver Biochemical Parameters in Sheep With Chronic Fascioliasis
(Univ Federal Santa Maria, 2022) Altug, Nuri; Basbugan, Yildiray; Yuksek, Nazmi
This study assessed changes in the levels of adenosine deaminase (ADA) and its isoenzymes in addition to routine liver biochemical parameters in sheep with fascioliasis. The study was conducted on 35 Akkaraman sheep. Of these, 25 sheep were diagnosed with fascioliasis based on anamnesis and clinical signs, and had endoparasites based on parasitological examinations (Fasciola-infected group). The remaining 10 sheep that were sampled from a single healthy herd (same flock) different from the infected group did not have any clinical signs or endoparasites (control group). Total protein (TP), albumin (ALB), and globulin (GLB) levels gradually increased on days after treatment compared to the values measured before treatment; the increases were statistically significant on all days for TP levels but only on day 14 after treatment for GLB levels (P < 0.05). Although, the ALB levels did not increase significantly on days after treatment, the ALB level and ALB/GLB ratio on days 7 and 14 after treatment were still lower than the values of day 21 after treatment and control group (P < 0.05). Total bilirubin (T-Bil) and direct bilirubin (D-Bil) levels on days 14 and 21 were significantly lower than that of day 0 (before treatment) and day 7 after treatment (P < 0.05). These results indicated that the increase in adenosine deaminase (ADA) and ADA1 levels may be due to possible concomitant infection of Fasciola larvae (in the parenchyma) and adults (in the bile duct).
Determination of Adenosine Deaminase Activity in Cattle Naturally Infected With Theileria Annulata
(Springer, 2008) Altug, Nuri; Yuksek, Nazmi; Agaoglu, Zahid Tevfik; Keles, Ihsan
The purpose of this study was to determine serum ADA activity in cattle naturally infected with Theileria annulata. In this study, a total of 37 cross-bred cattle which 27 of it showing clinical signs of theileriosis constituted infected group and 10 healthy cattle as control group were used as animal materials. Infected group divided into three groups according to their PCV values. Cattle with PCV >= 25 were put on group I (n=9), those with PCV 13-24 were put on group II (n=11) and those with PCV >= 12 were put on group III (n=7). Microscopical diagnosis of the disease was also made. Hematological parameters, serum enzyme activities (ADA, AST, ALT and ALP) were determined in all cattle. Hematological results revealed that significant progressive decreases in HGB, PLT, PBML counts and ratios from group I onwards to group III, whereas the WBC, PBPL counts and ratios showed an increase from group I onwards to group III. The serum ADA, AST, ALT and ALP activity increased significantly in all infected groups compared to control group. However, these parameters were also observed to decrease progressively from group I to group III. Furthermore, the highest increase in enzyme activities observed in the infected group I. But, these enzyme's activities started to decrease in infected group II and III in parallel with PBML and PLT counts. Eventhough, this decrease did not reach to the values obtained from control group. On the contrary, PBPL counts and ratios increased in infected group II and III in contrast to decrease in PCV. As a result, increased serum ADA activity in tropical theileriosis may reflect the involvement of the cellular immune responses.
Development of Pmp Gene-Specific Pcr Assay With a Host Specific Internal Control for Chlamydophila Felis
(Chulalongkorn Univ, 2014) Cantekin, Zafer; Solmaz, Hasan; Altug, Nuri; Ozmen, Gamze Ozge
Chlamydophila felis is the primary bacterial agent of conjunctivitis and upper respiratory disease in cats. Transmission of the disease requires close contact between cats. Polymerase chain reaction is a useful tool for detection of this organism. The aim of this study was to develop a polymerase chain reaction assay with an internal amplification control for the detection of C. felis. Primer pairs were designed specifically for polymorphic membrane protein gene of C. felis and cytochrome b gene of cat, and their specificity and sensitivity were examined. Primers specific for both genes were then multiplexed. In the simplex polymerase chain reaction analyses with 10-fold dilutions, C. felis DNA was detected with designed primers for polymorphic membrane protein genes up to 1.6 pg/mu l and cat DNA was demonstrated in all samples in the polymerase chain reaction. Moreover, in the multiplex polymerase chain reaction, C. felis DNA and cat DNA were detected together. These designed primers specific for C. felis might have potential for research on infections and shedding of this organism in cats as the internal control host specific primers might have potential for using internal control for detection of different microorganisms in cats.
Diagnosis and Treatment of Umbilical Cord-Derived Tetanus in Neonatal Calves
(Tubitak Scientific & Technological Research Council Turkey, 2017) Altug, Nuri; Yuksek, Nazmi; Karasu, Abdullah; Ilhan, Fatma; Ceylan, Ebubekir; Ekin, Ismail Hakki; Arslan, Sezai
In this study, umbilical cord-derived neonatal tetanus in calves was identified in Turkey. Four calves with tetanus-specific history and clinical findings were used. Blood samples were taken before and after treatment, and clinical findings were recorded. A tetanus-specific treatment procedure was performed. However, the calves died from not responding to treatment. Pretreatment white blood cell, peripheral blood polymorphonuclear leukocyte, creatine kinase (CK), and lactate dehydrogenase (LDH) levels were determined to be high. Aspartate aminotransferase, alanine aminotransferase, CK, and LDH activities were found to increase after treatment compared to before treatment. Gram-positive terminal spore-forming bacilli were observed in bacterioscopic examination of the necrotic tissue and swab samples from the umbilical cord region. The umbilical cord region was determined to be infected in all calves. In histopathological examination, pyogranuloma formation was observed in the wound area, and in Gram staining agents morphologically concordant with Clostridium tetani in necrotic material were observed. Blood serum of the calves was inoculated into mice. All mice died within 2 days after the inoculation, showing tetanus-specific clinical findings. As a result, bacterioscopy and histopathology of the umbilical cord region may be useful for diagnosis in addition to clinical findings. Mice trials may be used in confirming the diagnosis.
Diagnostic and Prognostic Value of Procalcitonin (Pct), C Reactive Protein (Crp), Nitric Oxide (No) Levels, and Adenosine Deaminase (Ada) Activity in Sheep With Natural Babesiosis Before and After Treatment
(Tubitak Scientific & Technological Research Council Turkey, 2018) Arslan, Sezai; Altug, Nuri; Muz, Mustafa Necati; Yuksek, Nazmi; Basbugan, Yildiray; Oruk Kilic, Ozlem
This study was carried out to reveal the importance of procalcitonin, C reactive protein, nitric oxide levels, and adenosine deaminase activity in the diagnosis and prognosis of the disease in naturally infected sheep with Babesia ovis. Thirty sheep diagnosed clinically and parasitologically as having Babesia ovis were allocated to 2 groups. The first group was treated only with imidocarp dipropionate and the second group with imidocarp dipropionate and flunixine meglumin. On the seventh day after treatment, blood samples were collected again from the sheep in the babesiosis-infected group and the treatment responses were assessed. Serum PCT (1.72 +/- 0.34 ng/mL, P < 0.01), CRP (101.42 +/- 11.73 mu g/mL, P < 0.001), NO (15.77 +/- 2.75 mu mol/L, P < 0.01), and ADA (13.92 +/- 0.88 IU/L, P < 0.01) were higher in sheep with babesiosis than in the healthy sheep (0.49 +/- 0.04 ng/mL, 49.46 +/- 4.57 mu g/mL, 8.15 +/- 0.63 mu mol/L, 9.34 +/- 1.19 IU/L, respectively). When PCT, CRP, NO, and ADA before treatment and after treatment in the infected sheep were compared, the levels of these parameters except for ADA in the second group were determined to have statistically decreased after the treatment. As a result, it has been concluded that the measurements of PCT, CRP, NO, and ADA in sheep with babesiosis may be useful for the diagnosis and prognosis of the disease when assessed in association with clinical examination.
Effect of Meloxicam and Flunixin Meglumine on Some Kidney Parameters in Geriatric Male Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2024) Komuroglu, Ahmet Ufuk; Basbugan, Yildiray; Yuksek, Nazmi; Altug, Nuri; Ozdek, Ugur; Dede, Semiha
Advanced age, called geriatrics, negatively affects the aging- related physiological changes on bodysystems. non-steroidal anti- inflammatory drugs (NSAIDs) used in geriatric pets or humans have negative effects on the kidneys. For this purpose, , the effects of Meloxicam and Flunixin Meglumine, which are NSAIDs frequently used in Veterinary Medicine, on the liver and kidney structure and function in geriatric male rats were evaluated. Twenty-four male geriatric rats (30-36 month old) and twenty-four 3-month-old young male Wistar albino rats were used in the study. Six groups were created, with eight rats in each group: young control (YC), young Meloxicam (YM), young Flunixin Meglubine (YFM), geriatric control (GC), geriatric meloxicam (GM), geriatric Flunixin Meglubine (GFM). Control groups (YC and GC) received an intraperitoneal injection of saline using the same volume as in the othergroups. Meloxicam was administered at 5.8 mgkg-1to-1 to the YM and GM groups, and Flunixin Meglumine at 2.5 mgkg-1-1 intraperitoneally to the YFM and GFM groups once a day for 5 days. Neutrophil Gelatinase-Associated Lipocalin (NGAL), Cystatin C (Cyc-c), Kidney Injury Molecule-1 (KIM-1), Interleukin-18 (IL-18), Urea, Creatinine (Crea), Albumin (Alb), and Total Protein (TP) levels, were determined in sera and urine samples. Serum NGAL, Cys-C, and KIM-1 levels in the GC group were found to be significantly higher than those in the YC group (P<0.05). P <0.05). Administration of both NSAIDs caused an increase in serum Cyc-c and NGAL levels in both young and geriatric rats (P<0.05). P <0.05). Since both Meloxicam and Flunixin Meglumine administration caused an increase in NGAL and Cys-c levels in young and geriatric rats, adjusting the drug dose and frequency of administration by evaluating the pretreatment renal function should be considered as a preventive measure.
Evaluation of the Levels of Homocysteine, Troponin I, and Nitric Oxide in Lambs With Subclinical White Muscle Disease
(Kafkas Univ, veteriner Fakultesi dergisi, 2011) Kozat, Suleyman; Altug, Nuri; Yuksek, Nazmi; Ozkan, Cumali
White muscle disease (WMD) or Nutritional myodegeneration disease (NMD) is a degenerative disease of the cardiac and skeletal muscles. The level of serum activity of Plasma homocysteine (Hcy), cardiac troponin I (cTn I), Nitric oxide (NO) were investigated in lambs with subclinical NMD in this study. Ten healthy lambs and twenty lambs with subclinical NMD were used in this study. The blood samples were firstly taken at the onset of the disease and then 3 and 10 days after treatment in NMD, and taken once control group. The values of serum activity of creatin kinase (CK), aspartate aminotranferase (AST), alanine aminotranferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were measured with autoanalyzer. Plasma concentration of Hcy, serum cTn I, and levels of NO were determined with ELISA method and value of activity erythrocyte glutathione peroxidase (GSH-Px) was measured with spectrophotometer. Before treatment, values of plasma Hcy, serum NO, cTn I, AST, ALT, ALP, CK, and LDH in lambs with NMD were higher than those of healthy ones (P<0.001), while GSH-Px activity of lambs with NMD are lower than those of healthy (P<0.001). After ten days of treatment, there were no significant differences between treated lambs with NMD and healthy lambs. Nutritional myodegeneration in lambs is associated with increased levels of Hcy, cTn I, and NO. Increased levels of Hcy, cTn I, and NO may be a result of selenium deficiency causing myocardial disorder in NMD lambs.
Immunostimulatory Effects of Aloe Vera and Β-Glucan on Cellular and Humoral Immune Responses Following Vaccination With Polyvalent Vaccines in Dogs
(Kafkas Univ, veteriner Fakultesi dergisi, 2010) Altug, Nuri; Yuksek, Nazmi; Agaoglu, Zahid Tevfik
The aim of this study was to evaluate the effects of Aloe vera and beta-Glucan on lymphocyte subsets, haematological parameters and immunglobulin concentrations following vaccination in dogs. For this purpose, totally 20 street dogs were used. The animals were allocated into five groups. Group 1 consisted of only polyvalent vaccine applied dogs. Group 2 and 3 were only Aloe vera and beta-Glucan applied dogs, respectively. Group 4 and 5 were consisted of polyvalent vaccine in addition to Aloe vera and beta-Glucan applied dogs, respectively. Blood samples were collected before vaccination and after vaccination (AV). Although platelet (PLT) counts in group 1 decreased AV, in all other groups (group 2, 3, 4, 5) it increased. White blood cell (WBC) and peripheral blood mononuclear lymphocyte (PBML) counts (in all groups), peripheral blood polymorphonuclear lymphocyte (PBPL) counts (group 1, 2, 4, 5), neutrophil (group 1, 4, 5), monocyte (group 2, 3, 5), packed cell volume (PCV) ratios (group 2 and 3) and haemoglobin (HGB) concentrations (group 3) increased AV. But, lymphocyte ratios in all groups did not change AV. CD8 T lymphocyte ratios in all groups increased at 7(th) and 14(th) days AV. Although CD3 T lymphocyte ratios in group 1 increased at 7(th) day AV, CD4 T and B lymphocytes ratios did not change AV period. But, both CD3 and CD4 T lymphocytes ratios in all other groups increased at 7th and 14th days AV. Moreover, B lymphocytes ratios in all groups except for group 1 increased AV. On the other hand, B lymphocytes ratios in group 2 and 3 increased only 7(th) day AV, while this increase in group 4 and 5 were present on both 7(th) and 14th days AV. Serum IgM concentrations in group 1 increased only 7(th) day AV, whereas its levels did not change in group 2 and 3. Serum IgG concentrations in group 1, 2 and 3 increased only 14(th) day AV. But, serum IgM and IgG concentrations in group 4 and 5 increased at 7(th) and 14(th) days AV. Administration of Aloe vera and beta-Glucan after vaccination in dogs may stimulate both cellular and humoral immun responses. Additionally, it might have restorative effect on PLT counts.
Investigation on the Relationship Between Lymphocyte Subsets, Immunoglobulin Levels and Adenosine Deaminase Activities in Immunosuppressive Dose Methylprednisolone Treated Dogs
(Natl veterinary Research inst, 2007) Altug, Nuri; Agaoglu, Zahid Tevfik
Seven healthy dogs were administered with methylprednisolone orally (2 mg/kg body weight/d) at the beginning of the study (between 0-10 d), then the dosing was decreased at 10 d intervals throughout 10 to 60 d in an alternate-day therapy programme. Drug administration to the dogs was stopped on the 60 d. Total leukocyte count; polymorphonuclear leukocytes, and neutrophil ratios increased and eosinophil, mononuclear leukocytes, lymphocyte, and monocyte ratios were decrease when these values were compared to changes between days. Furthermore, in lymphocyte subsets, B lymphocytes and CD 8 T lymphocytes ratios were found to decrease. In addition, CD 3 T lymphocyte ratios decreased on the 61 d and CD 4 T lymphocyte ratios increased on the 21, 41, 51 and 61 d. IgM and IgG levels decreased between the days. Serum total ADA and ADA 1 activities decreased during the application, but ADA 2 activity did not change significantly. As a result, after immunosuppressive dose of methylprednisolone application, reduction in the ADA and ADA 1 activity can be used as determinant of the suppression of immune system components.
The Levels of Trace Elements and Selected Vitamins in Goats With Chronic Fluorosis
(Tubitak Scientific & Technological Research Council Turkey, 2013) Altug, Nuri; Arslan, Sezai; Yuksek, Nazmi; Keles, Ihsan; Yoruk, Ibrahim Hakki; Basbugan, Yildiray; Aytekin, Ismail
This study was conducted to investigate the effects of chronic fluorosis on trace elements and vitamin levels in goats. Thirty-three goats exhibiting clinical signs of chronic fluorosis and 10 healthy goats from the same region were used. Animals with chronic fluorosis were divided into 2 groups according to their age as 1-3 years old and 4-6 years old. Blood samples were collected from all goats. Fluoride concentrations were determined with an ionometer. Na, K, and Cl levels were measured with ion-selective equipment and serum mineral substance levels were obtained by atomic absorption spectrophotometer. Ca, Mg, and P levels were determined by biochemistry analyzer. Serum vitamin concentrations were determined by liquid chromatography. The levels of serum copper, iron, manganese, and nickel decreased in goats with chronic fluorosis, whereas other trace elements did not change. Among vitamins, levels of serum a-tocopherol were increased, whereas retinol and vitamin D3 levels were not changed significantly differently between the fluorosis and control groups. In evaluation of goats with fluorosis according to age group, copper levels in the age group of 1-3 years and iron levels in the age group of 4-6 years were found to be lower than in the other age groups. Other minerals and all vitamin parameters were not different according to age. The results of the present study clearly showed that serum copper, iron, manganese, and nickel levels were reduced in goats with chronic fluorosis, and so goats with fluorosis should receive support in terms of these concerned trace elements.
Parasites Detected in Neonatal and Young Calves With Diarrhoea
(Natl veterinary Research inst, 2006) Goz, Yasar; Altug, Nuri; Yuksek, Nazmi; Ozkan, Cumali
This study was performed between December 2000 May 2005 to detect parasitic diarrhoea agents in neonatal and young calves. In this period, faecal samples were obtained from 231 neonatal and young calves. Age of the calves ranged from I day to 8 months. Faecal samples were examined using native Lugol's solution, Fulleborn's salty water technique and modified Ziehl-Neelsen staining technique for Cryptosporidium. The parasitological examination revealed that 188 (81.3%) of the calves were infected with one or more parasitic agents.
Relationship Between Degree of Anemia and Blood Gases in Cattle With Theileriosis
(Tubitak Scientific & Technological Research Council Turkey, 2014) Temiz, Mehmet; Altug, Nuri; Yuksek, Nazmi
This study was conducted to determine the relationship between the degrees of anemia and blood gases in cattle with theileriosis. It included 28 cattle with theileriosis and 7 healthy animals as the control group. The cattle with theileriosis were divided into 4 groups according to their hematocrit (Hct) values: cattle with Hct of >26 were considered as nonanemic (Group 1), Hct of 20-26 as mildly anemic (Group 2), Hct of 14-19 as moderately anemic (Group 3), and Hct of 10-13 as severely anemic (Group 4). The PCO2, HCO3, and TCO2 levels in the study groups were lower than those of the control group. Moreover, reductions in HCO3 and TCO2 concentrations were significant only in the severely anemic group. Similarly, the lowest PCO2 levels were also detected in the severely anemic group. There were no significant differences in the SO 2 levels between the control and study groups. On the other hand, the increase was significant in the severely anemic group when compared to Groups 1 and 2. The results seemed to indicate the trend of uncompensated metabolic acidosis in theileriosis cases with severe anemia; thus, veterinary surgeons should bear this in mind when evaluating the prognosis for such cases.
Relationship Between Packed Cell Volume Levels and Serum Nitric Oxide Concentrations in Cattle With Tropical Theileriosis
(Univ Agriculture, Fac veterinary Science, 2015) Ozkan, Cumali; Altug, Nuri; Akgul, Yakup; Keles, Ihsan; Kaya, Abdullah; Yuksek, Nazmi; Yur, Fatmagul
This study was performed to determine changes in serum NO (nitric oxide) levels in cattle with theileriosis in accordance with PCV levels. For this purpose, 42 cattle with theileriosis and 10 healthy cattle have been used as material. Infected animals have been divided into 3 equal groups. 1st group had <12%, 2nd group had 13-24% and 3rd group had <25% PCV level. Blood samples have been used to determine NO concentrations. Serum NO concentrations prior to treatment increased in all infected groups compared to control group however its levels were significant only in the third group. NO concentrations decreased to almost normal values after treatment especially in 2nd and 3rd group, however this decrease was not significant in the first group. As a result, NO levels have been found increased in cattle with natural theileriosis. Therefore, its concluded that NO level and PCV levels in theileriosis should be studied with more detail in the future to enlighten the role of NO release and strategies for controlling its' release should be made especially in severe cases. (C)2015 PVJ. All rights reserved
Rupture of the Gastrocnemius Muscle in a Cow Two Months After Twin Birth
(Natl veterinary Research inst, 2007) Altug, Nuri; Ozkan, Cumali; Yuksek, Nazmi; Karasu, Abdullah; Keles, Ihsan; Agaoglu, Zahid Tevfik; Ilhan, Fatma
The rupture of the gastrocnemius muscle was described in a cow who gave a twin birth. A tentative diagnosis of complete rupture of the muscle was based on information obtained from both anamnesis and clinical signs. The definitive diagnosis was made on necropsy. The role of serum minerals, especially Ca, changes in the muscle originated enzymes, and other laboratory parameters together with clinical examination findings was discussed.
Serum Adenosine Deaminase Activity in Domestic Animals: Reference Values
(Tubitak Scientific & Technological Research Council Turkey, 2009) Altug, Nuri; Yuksek, Nazmi; Agaoglu, Zahid; Keles, Ihsan
This study was aimed to determine normal serum adenosine deaminase (ADA) activity in domestic animals in both sexes and that were considered healthy on the basis of clinical and laboratory examinations. In this study, 262 non-pregnant animals of both sexes, belonging to 5 species [cattle (n = 50), sheep (n = 65), goat (n = 52), dog (n = 55), and cat (n = 40)] were used. Serum total ADA and ADA1 isoenzyme activities were found to be 6.34 +/- 0.31 IU/l and 6.34 +/- 0.31 IU/l in cattle, 7.57 +/- 0.30 IU/l and 7.31 +/- 0.47 IU/l in sheep, 7.52 +/- 0.36 IU/l and 6.44 +/- 0.72 IU/l in goat, 2.56 +/- 0.13 IU/l and 2.03 +/- 0.30 IU/l in dogs, 23.36 +/- 1.59 IU/l and 21.71 +/- 2.13 IU/l in cats, respectively. The serum levels of ADA2 were low in sheep, goats, dogs, and cats, whereas in these species ADA1 was high. Furthermore, there was no serum ADA2 activity in cattle. Additionally, there were no significant differences between genders in ADA activities in all species. As a result, determination of serum normal ADA and its isoenzyme activities in domestic animals will be useful for scientists who want to investigate ADA activity in future in different diseases.
Significance of Homocysteine and Cardiac Markers in Cattle With Hypocalcemia
(Tubitak Scientific & Technological Research Council Turkey, 2015) Basbugan, Yildiray; Yuksek, Nazmi; Altug, Nuri
This study aimed to determine the levels of homocysteine and cardiac markers in cows with hypocalcemia. This research was conducted with a total of 20 cows, including 10 with hypocalcemia and 10 healthy controls. Hypocalcemia was diagnosed by determining low blood calcium (Ca) levels, and a standard hypocalcemia treatment was initiated in those cows with the disease. Blood samples were taken before the treatment and again 12 h after the treatment, and there was no significant difference in the homocysteine levels between the groups. Ca, ionized calcium (ICa), magnesium (Mg), and phosphorus (P) levels were lower, while cardiac troponin I (cTnI), parathormone (PTH), creatine kinase (CK), and creatine kinase-MB (CK-MB) levels were higher before treatment as compared with the control group. Increases in the Ca, Mg, ICa, and P levels, and a decrease in the PTH levels were detected after treatment when compared with those levels before treatment. The CK, CK-MB, and Mg levels also increased, while the ICa level decreased in the aftertreatment group as compared with the control group. Therefore, the cTnI, CK, and CK-MB in cows with hypocalcemia were determined to be important diagnostically, but insignificant for prognosis. Homocysteine was not important for either diagnosis or prognosis.