Browsing by Author "Aras, Ibrahim"

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Assessment of Stromal Elastin Fibers in Breast Cancer and Fibroadenomas: Is There a Correlation With Ultrasound Elastography Findings
(Galenos Yayincilik, 2022) Toprak, Nursen; Aras, Ibrahim; Toktas, Osman; Yokus, Adem; Gunduz, Ali Mahir
Objective: The stiffness of a breast lesion provides information on the likelihood of malignancy. The most important factor affecting this stiffness is the composition of the extracellular matrix (ECM). The aim of this study was to assess the elastin fiber contents of malignant breast lesions and fibroadenomas and investigate any relationship between the shear wave velocity (SWV) measured by ultrasonography, and the elastin fiber content of lesions. Materials and Methods: Consecutive patients with breast lesions were enrolled. The SWV values of the lesions were analyzed. Histopathological analysis of elastin in excised lesions was performed by the method of Shivas and Douglas. Breast cancer patients were reviewed according to their lymph node status and tumor diameter. The relationship between SWV value and tissue elastin fiber score was analyzed. The correlation between breast cancer grade and elastin fiber score in malignant lesions was investigated. Results: A total of 167 consecutive breast lesions in 167 patients were included in this study (75 invasive cancer, 92 fibroadenomas). High elastic fiber score was significantly more common (p = 0.001) in malignant lesions (n = 61; 81.3%) than fibroadenomas (n = 13; 14.1%). There was a negative correlation between the mean SWV and the elastin fiber score of fibroadenomas (p = 0.001). A low grade in breast cancer was associated with high elastin fiber score (p = 0.01). Conclusion: Malignant lesions tend to have higher elastin fiber scores than fibroadenomas. Elastin fiber assessment may provide additional prognostic information in malignant lesions. Changes in elastin fiber content may account for the variation in elasticity in fibroadenomas.
Association Between Serum Cytokeratin 18 and N-Terminal Procollagen Iii Propeptide in Patients With Biopsy-Proven Nonalcoholic Fatty Liver Disease
(Taylor & Francis Ltd, 2024) Kaya, Sercan; Boydak, Murat; Aydin, Mesut; Aras, Ibrahim
Liver biopsy is still the gold standard in the staging of nonalcoholic fatty liver disease (NAFLD), which is the most common chronic liver disease worldwide. However, being an invasive method, liver biopsy has limited use in clinical practice. The aim of this study was to determine the relationship between serum levels of cytokeratin 18 (CK-M30) and N-terminal procollagen III propeptide (PIIINP) in patients with biopsy-proven NAFLD. The study was carried out on volunteers, including both healthy individuals and patients pre-diagnosed with NAFLD. The liver biopsies were re-assessed by applying the Steatosis, Activity, Fibrosis/Fatty Liver Inhibition of Progression (SAF/FLIP) algorithm. At the end of the study, frozen serum samples (-80 degrees C) were analyzed using commercial kits. CK18-M30 and PIIINP levels significantly differed in all study groups. There was no significant correlation between serum levels of CK18-M30 and PIIINP in healthy individuals but there was a significant positive correlation between CK18-M30 and PIIINP levels in NAFLD (NAFL-nonalcoholic steatohepatitis (NASH)) groups. CK18-M30 was better than PIIINP at distinguishing between NAFL and NASH. The results obtained for biopsy-proven NAFLD demonstrated that both PIIINP and CK18-M30 were partly associated with histological parameters and could aid in distinguishing between NASH and NAFL.
Energy Drinks May Affect the Ovarian Reserve and Serum Anti-Mullerian Hormone Levels in a Rat Model
(Galenos Yayincilik, 2021) Elci, Erkan; Elci, Gulhan Gunes; Cim, Numan; Aras, Ibrahim; Sayan, Sena; Yildizhan, Recep
Objective: Energy drinks have an impact on concentration levels, physical performance, speed of reaction, and focus, but these drinks cause many adverse effects and intoxication symptoms. The main goal of this study was to determine the effect of energy drink consumption on ovarian reserve and serum anti-mullerian hormone (AMH) levels. Materials and Methods: Female Wistar albino rats (n=16) were included and randomized into two groups (n=8). Serum AMH levels were checked before and after energy drinks were given. Eight weeks later, the ovaries and uteruses of the rats were analyzed histopathologically. The number of follicles in the ovaries was counted. Results: The total number of the preantral plus small antral follicles, which show the ovarian reserve, was decreased at the end of eight weeks in both the control group and the energy drink group. There was a statistical difference between them (p=0.021). Also, there was a statistically significant difference in the initial/final AMH (ng/mL) reduction levels between the control group and the energy drink group (p=0.002). AMH levels were decreased more in the energy drink group. Conclusion: The consumption of energy drinks can lead to a decrease in ovarian reserve and AMH values and may cause weight gain.
Neurotrophic Tyrosine Receptor Kinase (Ntrk) 1 and 2 Expression in Squamous Cell Carcinoma and Squamous Intraepithelial Lesions of the Cervix Uteri
(Mre Press, 2021) Erten, Remzi; Demir, Feyza; Alp, Hamit Hakan; Bayram, Irfan; Aras, Ibrahim
Background: Selective tropomyosin receptor kinase (Trk) inhibitors are known to provide promising outcomes in selected tumor pa-tients with neurotrophic tyrosine receptor kinase (NTRK) gene fu-sions. In the present study, we aimed to determine whether there was a NTRK1 and NTRK2 expression in cervical squamous cell car-cinoma (SCC) and precursor lesions. Materials and methods: A total of 92 formalin-fixed paraffin-embedded tissue samples of cervical SCC, squamous intraepithelial lesion (SIL) and non-neoplastic cervi-cal tissue (NCT) were subjected to immunohistochemical (IHC) stain-ing with NTRK1 and NTRK2 antibodies. These stainings were com-pared with p16/Ki-67 (IHC) and the low-risk/high-risk Human papillo-mavirus (HPV) in situ hybridization stainings that were previously ad -ministered. Results: In IHC analysis, NTRK1 expression was detected in 3.2% and 12.5% of SCC and cervical intraepithelial neoplasia (CIN)-2 samples, respectively. In addition, NTRK2 expression was detected in 6.5% and 6.3% of SCC and CIN-2 samples, respectively. However, NTRK1/NTRK2 expression was not detected in samples of NCT, CIN-1, and CIN-3. The p16 and Ki-67 expression and high-risk HPV posi-tivity increased as the grade of the lesion increased. Conclusions: The results indicated that both NTRK1 and NTRK2 had no contributory ef-fect on the grading and differentiation of cervical SCC and SIL. We consider that the IHC method is likely to be used for the screening of NTRK gene fusions in patients with cervical SCC. Trk inhibitors are likely to be a favorable therapeutic alternative for this low number of SCC cases with positive NTRK1/NTRK2 staining. Further studies are needed to confirm these ideas.
Protective Effects of Saffron, Safranal and Crocin Administration on Vitamins (A, D, E, K) and Protein Carbonyl Levels Against Cci4-Induced Oxidative Damage in Rats
(Marmara Univ, Fac Medicine, 2024) Bakir, Ahmet; Yildiz, Damla; Ekin, Suat; Oto, Gokhan; Aras, Ibrahim; Bayram, Irfan
Objective: The possible effects of saffron and its active components on oxidative stress are known. Protein carbonyls (PCO), formed due to protein exposure to oxidizing agents, are a newly researched topic. In this study, it was aimed to determine, antioxidant fatsoluble vitamins (A, D, E, K) and PCO values after saffron, safranal and crocin administration with carbon tetrachloride (CCl4) in rats. Materials and Methods: Fifty-four Wistar albino male rats were randomly selected, and 9 groups of n=6 were formed. Vitamin levels in rat serum were determined by HPLC and PCO levels were determined by spectrophotometric method. Results: A significant difference (p<0.01) was found between the CCl4 and the saffron, safranal and crocin groups. A significant decrease was observed in retinol and cholecalciferol values between CCl4 and saffron group (p<0.05, p<0.001), and a significant decrease in cholecalciferol and phylloquinone levels between CCl4 and safranal groups (p<0.01, p<0.05). Moreover, a decrease in cholecalciferol level (p<0.05) was determined between the olive oil, saffron and CCl4+crocin groups. Conclusion: As a result, saffron and safranal have a protective effect against CCl4-induced oxidative damage to PCO, retinol, phylloquinone and cholecalciferol, and this effect may be due to the potent antioxidative effects of saffron and safranal.
Therapeutic Plasma Exchange in Gastric Signet Ring Cell Carcinoma Presenting as Microangiopathic Hemolytic Anemia: a Rare Case Report
(Wiley, 2022) Candar, Omer; Ekinci, Omer; Merter, Mustafa; Aslan, Mehmet; Aras, Ibrahim
Microangiopathic hemolytic anemia (MAHA) defines a group of disorders characterized by the formation of microthrombi in capillaries and arterioles and the fragmentation of erythrocytes that pass through. Cancer-related MAHA is a rare but serious condition that is encountered in patients diagnosed with a malignancy. This clinical picture is thought to be linked to certain tumor characteristics; particularly, adenocarcinoma histology, vascular invasion, and bone marrow infiltration. MAHA is most commonly associated with tumors of gastric, prostate, and breast origin. The optimal treatment is not clear; however, there is evidence for the importance of promptly starting an effective antineoplastic regimen and it was also reported that administering therapeutic plasma exchange (TPE) therapy for immunocomplex removal could be beneficial for patients with symptoms of bleeding and thrombosis. Here, we present a case that presented a picture of MAHA secondary to gastric signet-ring cell adenocarcinoma (SRCC). The clinical picture was initially evaluated as thrombotic thrombocytopenic purpura and the patient benefited significantly from the TPE treatment administered before the adenocarcinoma diagnosis was confirmed. In this period, epistaxis stopped, platelet count increased from 25 x 10(9)/L to 162 x 10(9)/L, fragmented erythrocyte rate in the peripheral smear decreased by more than 75% and other laboratory findings of hemolysis (LDH, bilirubin, etc.) significantly improved.
An Unusual Complication Following Saphenous Vein Graft Harvesting for Coronary Artery Bypass Surgery: Pseudo-Kaposi Sarcoma
(Baycinar Medical Publ-baycinar Tibbi Yayincilik, 2020) Sahinalp, Sahin; Aras, Ibrahim
Pseudo-Kaposi sarcoma is a rare, vasoproliferative skin disease usually caused by an underlying vascular malformation or venous insufficiency. Unusual forms such as amputation stumps and lower extremity paralysis have been also reported. However, its emergence in the leg from which the saphenous vein was harvested following peripheral artery disease and coronary artery bypass surgery has not been previously reported. We, herein, present a 78-year-old female case who developed pseudo-Kaposi syndrome in her left leg, which was affected by peripheral artery disease following the removal of the saphenous vein six months after coronary artery bypass grafting.