Browsing by Author "Ari, E."

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Antioxidant and Renoprotective Effects of Paricalcitol on Experimental Contrast-Induced Nephropathy Model
(British inst Radiology, 2012) Ari, E.; Kedrah, A. E.; Alahdab, Y.; Bulut, G.; Eren, Z.; Baytekin, O.; Odabasi, D.
Objectives: The aim of the study was to assess the effect of paricalcitol on the experimental contrast-induced nephropathy (CIN) model. We hypothesised that paricalcitol may prevent CIN. Methods: 32 Wistar albino rats were divided into four groups (n=8 each): control group, paricalcitol group, CIN group and paricalcitol plus CIN group. Paricalcitol (0.4 mu g kg(-1) day(-1)) was given intraperitoneally for 5 consecutive days prior to induction of CIN. CIN was induced at day 4 by intravenous injection of indometacin (10 mg kg(-1)), N omega-nitro-L-arginine methyl ester (L-NAME, 10 mg kg(-1)) and meglumine amidotrizoate (6 ml kg(-1)). Renal function parameters, oxidative stress biomarkers, histopathological findings and vascular endothelial growth factor (VEGF) immunoexpression were evaluated. Results: The paricalcitol plus CIN group had lower mean serum creatinine levels (p=0.034) as well as higher creatinine clearance (p=0.042) than the CIN group. Serum malondialdehyde and kidney thiobarbituric acid-reacting substances levels were significantly lower in the paricalcitol plus CIN group than in the CIN group (p=0.024 and p=0.042, respectively). The mean scores of tubular necrosis (p=0.024), proteinaceous casts (p=0.038), medullary congestion (p=0.035) and VEGF immunoexpression (p=0.018) in the paricalcitol plus CIN group were also significantly lower. Conclusion: This study demonstrates the protective effect of paricalcitol in the prevention of CIN in an experimental model.
Arteriovenous Fistula Aneuryms Seen More Frequently at Brachial Region in End Stage Renal Failure Patients
(2011) Odabaşi, D.; Ari, E.; Ekim, H.
Objective: The aim of this study is to evaluate the aneursysm developing in arteriovenous fistula's (AVF) in end stage renal failure (ESRF) patients and to emphasize the importance of using the distal portions of extremities for AVF operations as possible. Material and Methods: Between January 2006 and January 2011 200 patients who have ESRF disease had 232 AVF operations. AVF operation conducted 113 (56%) of the patients were male and 87 (44%) were female. 17 (62%) of the patients were male and 10 (38%) of the patients were female who had anuerysm developed. Patients evaluated according to their sex, age, ESRF duration, AVF localizations. Results: The mean age of male patients was 41.50 ± 16.53, and female patients was 43.37 ±17.65 who had aneurysm in AVF's. The mean duration ESRF of male patients was 53±26 months and female patients was 56 ± 24 months. AVF localizations of 27 patients who had aneurysms are; right radial AVF 2 (7%), right high AVF 2 (7%), right brachial AVF 8 (29%), left radial AVF 1 (3%), left high radial AVF 5 (18%), left brachial AVF 9 (33%). The mean fistula flow of the male patients was 1332 ± 411 mL/min and female patients was 1117 ± 326 mL/min. Aneurysm developed 17 (15%) in 113 male patients, 10 (11%) in 87 female patients. High output cardiac failure developed 2 (11%) in 17 aneurysm developed male patients, 1 (10%) in 10 aneurysm developed female patients. Conclusion: Aneurysm developed significantly more in brachial region compared to forearm. It is the forearm if feasible vessels recieved to be preferred for AVF operation having regard the risk of aneursym development. Copyright © 2011 by Türkiye Klinikleri.
Increased Oxidative Stress in Living Kidney Donors: Correlation of Renal Functions With Antioxidant Capacity
(Elsevier Science inc, 2017) Yildirim, M.; Karahan, M.; Kucuk, H. F.; Demir, T.; Demir, H.; Turan, H.; Ari, E.
Background. Substantial attention has recently been paid to the possibility of an increased risk of chronic kidney disease (CKD) in living kidney donors. It has been demonstrated that CKD patients suffer from increased oxidative stress, which have been reported to show a strong association with several clinical problems such as accelerated atherosclerosis. The purpose of the current cross-sectional, single-center study was to evaluate the relationship between renal functions of living kidney donors and systemic oxidative stress. Methods. A total of 55 living kidney donors operated at least 1 year ago and 40 age and sex-matched healthy individuals were enrolled in this study. Plasma malondialdehyde (MDA) levels were determined as oxidative stress marker. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured as antioxidants. Renal function parameters and proteinuria were also assessed. Results. Mean serum creatinine levels were higher (P = .022) and 24-hour creatinine clearance was lower (P = .016) in living kidney donors compared with healthy controls. Serum MDA levels were higher (P = .034), and SOD and GPx activities were lower (P = .023 and P < .001, respectively). There was a significant positive correlation between serum GPx activity and 24-hour creatinine clearance levels (r = 0.524, P < .01). A linear regression analysis showed that serum GPx activity was still significantly and positively correlated with creatinine clearance (regression coefficient = 0.416, P < .001). Conclusion. Our data demonstrated that living kidney donors exhibit slightly reduced kidney function, increased oxidative stress, and decreased antioxidant activity. We propose that 24-hour creatinine clearance is positively correlated with antioxidant enzyme GPx. To our knowledge, this is the first study to demonstrate the association between renal functions and antioxidant activity in kidney donors.
Oxidative Dna Damage Is Increased in Living Kidney Donors
(Elsevier Science inc, 2019) Karahan, M.; Yildirim, M.; Kucuk, H. F.; Turunc, V; Demir, H.; Salturk, C.; Ari, E.
Background. Long-term consequences of donor nephrectomy might be reduced kidney function, increased risk for cardiovascular disease, and impaired quality of life. The purpose of the current cross-sectional study was to evaluate the relationship between clinical, laboratory, and donation-specific outcomes of living kidney donors and systemic oxidative DNA damage. Methods. We conducted a cross-sectional study and assessed retrospectively pre- and postdonation data from 60 donors who donated between 2010 and 2015. Plasma malondialdehyde levels and 8-hydroxy-20-deoxyguanosine/deoxyguanosine ratio (8-OHdG/dG ratio) were determined as oxidative stress markers. Catalase, carbonic anhydrase, and paraoxonase (PON) activities were measured as antioxidants. Results. Approximately 3 years after donation, the hypertensive donor ratio was 12%, and 11% of the donors had glomerular filtration rate <60 mL/min/1.73 m(2). Mean serum urea (P =.001) and serum creatinine levels (P =.001) were increased; creatinine clearance level (126.2 +/- 35.5 vs 94.6 +/- 26.8, P =.001) was decreased in the postdonation period. There was a significant positive correlation between predonation serum urea and 8-0HdG/dG ratio (r = 0.338, P =.016) and predonation serum creatinine and 8-0HdG/dG ratio (r = 0.442, P =.001), while there was a significant negative correlation between serum creatinine and PON activity (r = -0.545, P <.001). Conclusion. Our data have demonstrated that kidney donors exhibit increased oxidative DNA damage and decreased antioxidant activity. We propose that predonation serum creatinine is positively correlated with 8-0HdG/dG ratio and negatively correlated with antioxidant PON activity. This is the first study to demonstrate that plasma oxidative DNA damage increases in healthy kidney donors.
Should We Use Basilic Vein Transposition Instead of Prosthetic Graft for Creation of Secondary Arterio-Venous Fistula in Hemodialysis Dependent End Stage Renal Failure Patients
(2011) Odabaşi, D.; Ekim, H.; Ari, E.
Objective: Every end stage renal failure (ESRF) patient needs vascular access for hemodialysis (HD). Secondary arterio-venous fistula (AVF) constitution with prosthetic graft (PG) or basilic vein transposition (BVT) are good alternatives for patients who lost the chance of radio-cephalic arterio-venous fistula (RCAVF) or brachio-cephalic arterio-venous fistula (BCAVF). To evaluate the patency and complication rates after AVF formation a concurrent series of patients were reviewed. Material and Methods: From January 2006 to January 2010, 40 secondary HD access procedures were performed in 40 consecutive patients. All access procedures were planned on the basis of preoperative duplex ultrasonography (USG) scans of arm and forearm. Functional patency was defined as ability to cannulate for HD patient successfully. Primary and secondary cumulative functional patency of PG's and BVT's were determined with Kaplan Meier test, differences were analyzed with Log Rank test, differences in revision rates including thrombolysis, thrombectomies and operative revisions were analyzed with the Z test and the Fisher exact t-test. Results: Mean follow up was 48 (43-54 months) months. Risk factors were similar between the two groups. BVT group has the better patency ratings. Steal syndrome and odema were higher in BVT group. Conclusion: As long as the patient is a candidate for an upper arm secondary AVF creation based on anatomical criteria BVT is always be considered before a PG. Copyright © 2011 by Türkiye Klinikleri.