Browsing by Author "Arihan, Seda Karaoz"

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Effect of Acute and Chronic Fluoride Administration on Bone Histopathology, Bone Fluoride Accumulation, and Locomotor Activity in an Animal Model of Paleopathological Fluorosis
(int Soc Fluoride Research, 2020) Turkekul, Ramazan; Arihan, Seda Karaoz; Yildirim, Serkan; Arihan, Okan; Oto, Gokhan; Ekin, Suat; Yildiz, Damla
Fluorosis may be identified in skeletal materials from ancient civilizations by macroscopic signs in teeth and bone and hard tissue fluoride levels. In the present study, human teeth, femoral, and rib specimens from the Van Fortress excavation, in Turkey, were examined for the presence of fluorosis. In addition, an animal study in rats was conducted as a model of human fluorosis, by examining the effects of fluoride administration, in various doses and for different durations, on weight loss, locomotor activity, fluoride accumulation, and deformation in bone and teeth. Fifty-six adult male Wistar albino rats, weighing 150-200 g, were divided into 7 different groups of 8 rats. Four acute groups were treated with 0 (control), 5, 15, and 50 mg/L of fluoride in drinking water for 7 days and three chronic groups were treated with 5, 15, and 50 mg/L of fluoride for 90 days. The results of the analysis of the human samples from the Van Fortress excavation showed that none of the dental, femoral, or rib samples had a fluoride content that was significantly greater than that of the surrounding soil. The results of the rat study showed that no significant differences between the groups were found in body weight on days 1, 30, 60, and 90. The rotarod locomotor test showed a significant (p<0.05) dose- and time-dependent reduction in locomotor activity as a result of the fluoride administration in the 50 mg/L chronic fluoride group compared to the control, 5 mg/L acute fluoride, 15 mg/L acute fluoride, and 5 mg/L chronic fluoride groups. Significant fluoride accumulation was found in the femoral neck (cortical tissue), the femoral head (trabecular tissue), and in rib bone. Light microscopy showed a severe thinning of the epiphyseal growth plate and bone trabeculae in the femoral bone tissue. We concluded that femoral bone (cortical and trabecular parts) and ribs are good sites for assessing the effects of fluoride exposure in animal models of human fluorosis.
Effects of Acute and Chronic Fluoride Administration on Some Kidney Parameters of Rats (cys-C, Kim-1, and Ngal)
(int Soc Fluoride Research, 2021) Komuruglu, Ahmet Ufuk; Basbugan, Yildiray; Arihan, Okan; Arihan, Seda Karaoz
Fluorosis is an important disease both in modern societies as well as in the previous periods. Paleopathological studies reveal that people in ancient periods suffered from fluorosis which causes adverse health effects, especially on the musculoskeletal system as well as soft tissues such as kidneys. In this study, we aimed to investigate the effects of acute and chronic fluoride administration on some kidney markers in rats. Fifty-six Wistar albino rats were divided into 7 groups, 8 in each group. Acute fluoride intoxication was established by administering, in drinking water, 5 ppm (group 2), 15 ppm (group 3), and 50 ppm (group 4) for 7 days. Chronic fluoride intoxication was established by administering 5 ppm (group 5), 15 ppm (group 6), and 50 ppm for 90 days (group 7). Control group (group 1) was given tap water. At the end of the study, the rats were sacrificed under anesthesia and blood samples were taken. The blood was centrifuged and their serums were separated. CYS-c, KIM-1, and NGAL levels were measured by ELISA method, and urea, creatinine, total protein, and albumin levels were measured spectrophotometrically. CYS-c levels were increased in all groups administered fluoride (p>0.05). Similarly all groups had higher levels of NGAL due to fluoride exposure and chronic fluoride 5 mg/L group showed significant increase compared to control (p<0.05). In KIM-1 values, a significant increase occurred in acute fluoride 15 and 50 mg/L (p<0.05). Significant alterations were also observed in creatinine and urea values due to fluoride exposure. Consequently, exposure to fluoride may cause an increase in serum inflammation markers (NGAL, KIM-1) due to differences in dosage and exposure period. Further long term studies, including molecular and histopathological assessments, are needed to elucidate the impact of long term exposure to fluoride on the renal system.
Example of Digital Exhibition in Anthropology and Medical Education
(Taylor & Francis Ltd, 2023) Gul, Asli San Dagli; Arihan, Seda Karaoz; Arihan, Okan
Technology brings new opportunities in terms of education and research, and global pandemics such as the recent COVID-19 pandemic require online education-style approaches. Visualisation is of great importance in digitised education. In this study, materials that are of interest to the disciplines of anthropology, dentistry and medicine were selected from the skeletal materials and presented in a virtual exhibition. The mandible samples were digitised by photogrammetric method, and the exhibition was made navigable by using the Unity game engine program. In the exhibition, information on dental and bone physiology and palaeopathology, as well as the mandibles of the skeletons obtained from the excavation area, and information on individuals are presented on informative boards. The exhibition can be visited online with the link provided. In this study, the opinions of 30 students, who visited the exhibition from Faculty of Medicine 3(rd)-5(th) classes, were asked to complete an online questionnaire. Results of this questionnaire show that students would like to see more of such digital education methods. Digitising educational presentations enables these exhibitions to transcend physical boundaries and reach a global audience. In addition, the images can be easily examined by anyone who visits and can be evaluated for educational purposes.
Investigation of Effect of Exercise on Physical Parameters on Football Referees at Van Region
(Wiley-blackwell, 2015) Kina, Erol; Arihan, Okan; Erkec, Ozlem Ergul; Kara, Mehmet; Arihan, Seda Karaoz; Bektas, Yener