Browsing by Author "Arslan, Mehmet Enes"

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In Vitro Evaluation of Selective Cytotoxic Activity of Chaerophyllum Macropodum Boiss. on Cultured Human Sh-Sy5y Neuroblastoma Cells
(Springer, 2022) Celikezen, Fatih Caglar; Turkez, Hasan; Firat, Mehmet; Arslan, Mehmet Enes; Oner, Sena
Neuroblastoma is the most common solid tumor in children. New treatment approaches are needed because of the harmful side effects and costs of the methods used in the treatment of neuroblastoma. Medicinal and aromatic plants are important for new treatment approaches due to their minimal side effects and economic advantages. Therefore, the present study was carried out to examine the cytotoxic effect of Chaerophyllum macropodum extract on human neuroblastoma (SH-SY5Y) and fibroblast (HDFa) cell lines. 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase release (LDH) assays were used to determine the cytotoxic effect of C. macropodum. The extracts were analyzed for their phenolic content by HPLC-PDA. Major components were determined as 63.600% o-coumaric acid, 15.606% catechine hydrate, 8.713% rosmarinic acid, 4.376% clorogenic acid, and 3.972% salicylic acid. The obtained results from cytotoxicity testing revealed that C. macropodum exerted a significant cytotoxic effect on human neuroblastoma cells at all tested concentrations (p < 0.05). But it did not lead to any cytotoxic potential on human fibroblasts. As a result, the obtained data clearly revealed C. macropodum exerted a selective cytotoxic action on neuroblastoma cells for the first time.
Magnetic Nanoparticles-Induced Neurotoxicity and Oxidative Stress in Brain of Rainbow Trout: Mitigation by Ulexite Through Modulation of Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities
(Elsevier, 2022) Ucar, Arzu; Parlak, Veysel; Ozgeris, Fatma Betul; Yeltekin, Asli Cilingir; Arslan, Mehmet Enes; Alak, Gonca; Atamanalp, Muhammed
The prevalent exposition of metallic nanoparticles (MNPs) to the aquatic medium and their negative influence on human life is one of the major concerns global. Stress mechanization, as a non-specific and pervasive response, involves all physiological systems, particularly the closely interconnected neuroendocrine and immune systems. In this study, which was designed to obtain more data on the biological effects of ulexit, which prevents oxidative DNA damage by protecting against toxicity damage and offers new antioxidant roles. The concomitant use of ulexite (UX, as 18.75 mg/l) as a natural therapeutic agent against exposure to magnetic nanoparticles (Fe3O4-MNPs/0.013 ml/l) on Oncorhynchus mykiss was investigated for 96 h. The brain tissues were taken at the 48th and 96th hours of the trial period, the effects on neurotoxic, pro-inflammatory cytokine genes, antioxidant immune system, DNA and apoptosis mechanisms were analyzed. In the present study, it was determined that AChE activity and BDNF level in the brain tissue decreased over time in the Fe3O4-MNPs group compared to the control, and UX tried to depress this inhibition. While inhibition was determined in antioxidant system biomarkers (SOD, CAT, GPx, and GSH values), an induction was observed in lipid peroxidation indicators (MDA and MPO values) in Fe3O4-MNPs applied group. The same group data showed that TNF-alpha, IL-6, 8-OHdG and caspase-3 levels were increased, but Nrf-2 levels were decreased. The alterations in all biomarkers were found to be significant at the p < 0.05 level. In general, it was determined that Fe3O4-MNPs caused stress in O. mykiss and UX exhibited a positive effect on this stress management.
Neutralization of Iron Oxide Magnetic Nanoparticle Aquatoxicity on Oncorhynchus Mykiss Via Supplementation With Ulexite
(Taylor & Francis Ltd, 2024) Ucar, Arzu; Arslan, Mehmet Enes; Yeltekin, Asli Cilingir; Ozgeris, Fatma Betul; Yildirim, Ozge Caglar; Parlak, Veysel; Atamanalp, Muhammed
Nowadays, the unique features of nanoparticles (NPs) have encouraged new applications in different areas including biology, medicine, agriculture, and electronics. Their quick joining into daily life not only enhances the uses of NPs in a wide range of modern technologies but also their release into the aquatic environment causes inevitable environmental concerns. On the other hand boron exhibits key physiological effects on biological systems. This research was designed for evaluating the toxicity of magnetite nanoparticles (Fe3O4-MNPs) on aquatic organisms and obtaining data for the information gap in this area. In this study, Rainbow trout (Oncorhynchus mykiss) was considered as an aquatic indicator, and trials were designed as Ulexite (a boron mineral, UX) treatment against exposure to Fe3O4-MNPs. Synthesized and characterized Fe3O4-MNPs were exposed to rainbow trouts in wide spectrum concentrations (0.005-0.08 mL/L) to analyze its lethal dose (LC50) and cytoprotective properties by UX treatment were assessed against Fe3O4-MNPs applications for 96 h. For the initial toxicity analysis, hematological parameters (blood cell counts) were examined in experimental groups and micronucleus (MN) assay was performed to monitor nuclear abnormalities after exposure to NPs. Biochemical analyzes in both blood and liver samples were utilized to assess antioxidant/oxidative stress and inflammatory parameters. Also, 8-hydroxy-2'-deoxyguanosine (8-OHdG) assay was used to investigate oxidative DNA lesions and Caspase-3 analysis was performed on both blood and liver tissues to monitor apoptotic cell death occurrence. When antioxidant enzymes in blood and liver tissue were examined, time-dependent decreases in activity were determined in SOD, CAT, GPx, and GSH enzymes, while increased levels of MDA and MPO parameters were observed in respect to Fe3O4-MNPs exposure. It was found that TNF-alpha, Il-6 levels were enhanced against Fe3O4-MNPs treatment, but Nrf-2 levels were decreased at the 46th and 96th h. In the 96th application results, all parameters were statistically significant (p < 0.05) in blood and liver tissue, except for the IL-6 results. It was determined that the frequency of MN, the level of 8-OHdG and caspase-3 activity increased in respect to Fe3O4-MNPs exposure over time. Treatment with UX alleviated Fe3O4-MNPs-induced hematotoxic and hepatotoxic alterations as well as oxidative and genetic damages. Our findings offer strong evidence for the use of UX as promising, safe and natural protective agents against environmental toxicity of magnetite nanoparticles.
Toxic Releases and Exposure Assessment: a Multi-Endpoint Approach in Fish for Ferrocene Toxicity
(Elsevier, 2023) Ozgeris, Fatma Betul; Yeltekin, Asli Cilingir; Ucar, Arzu; Caglar, Ozge; Parlak, Veysel; Arslan, Mehmet Enes; Alak, Gonca
Fe2+ in ferrocene facilities the oxidation. Based on this phenomenon, increased iron (Fe) level in freshwater ecosystems is thought as an important environmental problem in many geographic regions. In addition to increased mobilization of Fe from sediment due to changes in land use, mining, industrial activity, and elevated acid deposition are also proposed to be possible factors contributing to the increased Fe loading in freshwater environments. Ferrocene is useful in the modern organometallic chemistry industry due to its versatile appli-cations. In this study, the toxicity potential and related toxicity mechanisms of acute ferrocene exposure as well as the protective potential of borax supplementation against ferrocene were investigated in rainbow trout during 96 h under semi-static conditions. In target tissues multiplexed endpoints of hematological indices, genotoxicity, oxidative stress response, DNA damage and apoptosis levels, as well as tumor necrosis factor alpha, and interleukin-6 activities were assessed in blood tissue. In liver tissue, in addition to the parameters studied in blood tissue (except cortisol), the nuclear factor erythroid-2, which regulates the expression of detoxification enzymes, was investigated. When the results obtained from blood analyzes were examined, ferrocen treatment caused different reactions (increase/decrease) in blood indexes, and these findings were confirmed by MN tests. In ferrocene-induced hematoxicite, the healing effect of borax application has been observed to increase inhibited values and decrease in indexes with increasing tendencies. Besides, this hematoxicity was also supported by cortisol in-creases. Our findings showed that ferrocene inhibited antioxidant enzyme activities and increased lipid perox-idation, 8-OH-dG, caspase 3, TNF-alpha, and IL-6 levels in both blood and liver tissues. Similarly, cortisol level (in blood tissue) and Nrf-2 level (in liver tissue) increased with ferrocene application. In the ferrocen+borax group, the MDA level decreased 11 % at the end of the 96th hour compared to the 48th hour, and the Nrf2 level increased 9 %. In general, enzyme inhibitions in blood and liver tissues have shown that ferrocen-mediated toxicity occurs in induced ROS, DNA damage, apoptos activity, and BX applications have a positive effect on the correction of toxicity in the direction of hormesis. In a conclusion, the present study suggested that borax migt exhibite ameliorative potential against ferrocene-induced toxicity in O. mykiss blood and liver via regulating the ROS/TNF-alpha/Nrf-2 pathway.