Browsing by Author "Arslan, Sezai"

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Diagnosis and Treatment of Umbilical Cord-Derived Tetanus in Neonatal Calves
(Tubitak Scientific & Technological Research Council Turkey, 2017) Altug, Nuri; Yuksek, Nazmi; Karasu, Abdullah; Ilhan, Fatma; Ceylan, Ebubekir; Ekin, Ismail Hakki; Arslan, Sezai
In this study, umbilical cord-derived neonatal tetanus in calves was identified in Turkey. Four calves with tetanus-specific history and clinical findings were used. Blood samples were taken before and after treatment, and clinical findings were recorded. A tetanus-specific treatment procedure was performed. However, the calves died from not responding to treatment. Pretreatment white blood cell, peripheral blood polymorphonuclear leukocyte, creatine kinase (CK), and lactate dehydrogenase (LDH) levels were determined to be high. Aspartate aminotransferase, alanine aminotransferase, CK, and LDH activities were found to increase after treatment compared to before treatment. Gram-positive terminal spore-forming bacilli were observed in bacterioscopic examination of the necrotic tissue and swab samples from the umbilical cord region. The umbilical cord region was determined to be infected in all calves. In histopathological examination, pyogranuloma formation was observed in the wound area, and in Gram staining agents morphologically concordant with Clostridium tetani in necrotic material were observed. Blood serum of the calves was inoculated into mice. All mice died within 2 days after the inoculation, showing tetanus-specific clinical findings. As a result, bacterioscopy and histopathology of the umbilical cord region may be useful for diagnosis in addition to clinical findings. Mice trials may be used in confirming the diagnosis.
Diagnostic and Prognostic Value of Procalcitonin (Pct), C Reactive Protein (Crp), Nitric Oxide (No) Levels, and Adenosine Deaminase (Ada) Activity in Sheep With Natural Babesiosis Before and After Treatment
(Tubitak Scientific & Technological Research Council Turkey, 2018) Arslan, Sezai; Altug, Nuri; Muz, Mustafa Necati; Yuksek, Nazmi; Basbugan, Yildiray; Oruk Kilic, Ozlem
This study was carried out to reveal the importance of procalcitonin, C reactive protein, nitric oxide levels, and adenosine deaminase activity in the diagnosis and prognosis of the disease in naturally infected sheep with Babesia ovis. Thirty sheep diagnosed clinically and parasitologically as having Babesia ovis were allocated to 2 groups. The first group was treated only with imidocarp dipropionate and the second group with imidocarp dipropionate and flunixine meglumin. On the seventh day after treatment, blood samples were collected again from the sheep in the babesiosis-infected group and the treatment responses were assessed. Serum PCT (1.72 +/- 0.34 ng/mL, P < 0.01), CRP (101.42 +/- 11.73 mu g/mL, P < 0.001), NO (15.77 +/- 2.75 mu mol/L, P < 0.01), and ADA (13.92 +/- 0.88 IU/L, P < 0.01) were higher in sheep with babesiosis than in the healthy sheep (0.49 +/- 0.04 ng/mL, 49.46 +/- 4.57 mu g/mL, 8.15 +/- 0.63 mu mol/L, 9.34 +/- 1.19 IU/L, respectively). When PCT, CRP, NO, and ADA before treatment and after treatment in the infected sheep were compared, the levels of these parameters except for ADA in the second group were determined to have statistically decreased after the treatment. As a result, it has been concluded that the measurements of PCT, CRP, NO, and ADA in sheep with babesiosis may be useful for the diagnosis and prognosis of the disease when assessed in association with clinical examination.
Effeciency of Various Supportive Treatments as a Cure for Anaemia in Cattle With Theileriosis
(Chulalongkorn Univ, 2014) Altug, Nun; Yuksek, Nazmi; Keles, Ihsan; Ozkan, Cumali; Yoruk, Ibrahim Hakki; Arslan, Sezai
The purpose of this study was to investigate the efficiency of various supportive treatments on the restoration of anaemia in theileriosis. A total of 20 cattle infected with theileriosis received intramuscularly a single dose of buparvaquone (2.5 mg/kg(-1) bw) and were divided into 4 equal groups according to the different therapy options: whereas no supportive treatment was applied in group 1, administrations of vitamin B complex, antioxidant vitamins (A, D, E and C), minerals and trace elements were performed in groups 2, 3 and 4, respectively. Haematological parameters, serum concentrations of antioxidant vitamins, minerals and trace elements as well as serum glutathion peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were determined before treatment and 7 days after in infected animals and compared to control values from 10 healthy cattle. In the theileriosis affected cattle, remarkable decreases in haematocrit values, haemoglobinaemia and platelet counts were associated to decreased serum alpha- / gamma-tocopherol, vitamin C, vitamin B-12, Ca, Mg, Zn, Na, GSH-Px and SOD levels and to increased sideraemia. The iron concentrations remained elevated and some antioxidants (alpha- / gamma-tocopherol and vitamin C concentrations and GSH-Px and SOD activities) decreased in all treated cattle. Haematocrit values, haemoglobinaemia and vitamin B-12 concentrations also remained depressed but maximal increases were recorded in cattle receiving vitamins B as supportive therapy (group 2). In parallel, platelet numeration was significantly restored in all groups, but specifically in group 2. On the other hand, the maximal increases in alpha-tocopherol and Zn concentrations recorded in groups 3 and 4, respectively, were not associated with cure of anaemia. These results show that the most efficient supportive therapy in the cure of anaemia during theileriosis was vitamin B administration.
The Levels of Trace Elements and Selected Vitamins in Goats With Chronic Fluorosis
(Tubitak Scientific & Technological Research Council Turkey, 2013) Altug, Nuri; Arslan, Sezai; Yuksek, Nazmi; Keles, Ihsan; Yoruk, Ibrahim Hakki; Basbugan, Yildiray; Aytekin, Ismail
This study was conducted to investigate the effects of chronic fluorosis on trace elements and vitamin levels in goats. Thirty-three goats exhibiting clinical signs of chronic fluorosis and 10 healthy goats from the same region were used. Animals with chronic fluorosis were divided into 2 groups according to their age as 1-3 years old and 4-6 years old. Blood samples were collected from all goats. Fluoride concentrations were determined with an ionometer. Na, K, and Cl levels were measured with ion-selective equipment and serum mineral substance levels were obtained by atomic absorption spectrophotometer. Ca, Mg, and P levels were determined by biochemistry analyzer. Serum vitamin concentrations were determined by liquid chromatography. The levels of serum copper, iron, manganese, and nickel decreased in goats with chronic fluorosis, whereas other trace elements did not change. Among vitamins, levels of serum a-tocopherol were increased, whereas retinol and vitamin D3 levels were not changed significantly differently between the fluorosis and control groups. In evaluation of goats with fluorosis according to age group, copper levels in the age group of 1-3 years and iron levels in the age group of 4-6 years were found to be lower than in the other age groups. Other minerals and all vitamin parameters were not different according to age. The results of the present study clearly showed that serum copper, iron, manganese, and nickel levels were reduced in goats with chronic fluorosis, and so goats with fluorosis should receive support in terms of these concerned trace elements.
The Relationship Between Thyroid Hormones and Fluoride Levels in Goats With Fluorosis
(2008) Arslan, Sezai; Keleş, İhsan
Bu çalışmada Tendürek Dağı (Ayrancı, Alıntepe ve Yılanlı köyü) ile Ağrı Dağı'nın (Gölyüzü ve Çiftlik köyü) eteklerinde yaşayan ve endemik florozisin görüldüğü yerlerdeki keçilerin flor ile tiroit hormonları (TT3, TT4, FT3 ve FT4) arasındaki ilişki araştırıldı. Araştırmanın materyalini 104 adet florozisli ve 30 adet sağlıklı keçi oluşturdu. Keçilerin flor düzeyleri spesifik flor elektrotu ile tiroit hormonları ise DPC İmmulite 2000 cihazıyla yapıldı. Diş florozisi belirtilerini gösteren keçiler florozis grubunu oluşturdu. Bu keçilerin % 1.92'sinde palpe edilebilir kemik ekzostozları, %19.23'ünde çiğneme güçlüğü ve zayıflık, %2.88'inde tırnak deformiteleri ve %7.69'unda topallık belirtileri tespit edildi. Ayrıca boyun bölgesinin inpeksiyon ve palpasyonunda guatr belirtilerine rastlanmadı. Yapılan laboratuar çalışmaları sonucunda florozis grubu keçilerde plazma flor düzeyi kontrol grubuna göre istatistiki olarak önemli (p<0.001) bir artış gösterdiği belirlendi. Ayrıca plazma flor düzeyinin yaşın ilerlemesiyle arttığı ve cinsiyetle ilişkisinin olmadığı ortaya konuldu. Florozisli keçilerde TT3, TT4 ve FT4'ün normale göre azaldığı, FT3'ün ise arttığı tespit edildi. Ancak bunlardan sadece TT4'deki farklılık istatistiki olarak önemli (p<0.01) bulundu. Ayrıca florozisli dişi keçilerin TT3 ve TT4 düzeylerinin erkek keçilere göre daha yüksek olduğu ve istatistiki açıdan önemli (p<0.05) olduğu tespit edildi. Köylere göre inceleme yapıldığında florozisin görüldüğü köylerdeki keçilerde flor düzeyinin normale göre yüksek olduğu ve istatistiki açıdan önem arz ettiği tespit edildi. Sonuç olarak; keçilerde flor düzeyinin yaş ilerledikçe arttığı ancak istatistiksel olarak önemli olmadığı, florozisin tiroit hormonlarından özellikle TT4'ün önemli derecede azalmasına neden olduğu ve florozisli keçilerde yaş arttıkça tiroit hormonlarının azaldığı bu çalışma ile Türkiye'de ilk kez ortaya konuldu.
Yeni Doğan Buzağıların İshal Olgularında Rotavirus ve Coronavirus Araştırılması
(2007) Kaya, Abdullah; Çabalar, Mehmet; Arslan, Sezai
Bovine rotavirus (BRV) ve bovine coronavirus (BCV) yeni doğan buzağılarda ishal olgularının başlıca viral etkenleridir. Bu çalışma, buzağıların ishal olgularında BRV ve BCV enfeksiyonlarının belirlenmesi amacıyla yapıldı. Bu amaçla, Van yöresindeki 89 ishalli buzağıdan alınan dışkı örnekleri, BRV için polyacrylamide gel electrophoresis (PAGE) tekniği ve BCV için enzyme linked immunosorbent assay (ELISA) kiti kullanılarak test edildi. Araştırma sonucunda, 89 ishalli buzağıdan 16 (%17.97)'sı rotavirus ve yalnızca 1 (%1.12) tanesi coronavirus yönünden pozitif tespit edildi. Elde edilen bulgular ile BRV ve BCV'ün yeni doğan buzağıların ishal olgularında etiyolojik faktör olarak rol oynayabileceği ortaya konuldu.