Browsing by Author "Arslantas, Ali"

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A Comparative Study on Dna Binding Properties of 2,10,16,24-Tetrakis 4-(4 Phenoxy)-Substituted Co(Ii) and Mg(Ii) Phthalocyanine Compounds
(Wiley-v C H verlag Gmbh, 2017) Arslantas, Ali; Agirtas, Mehmet Salih
In this study, DNA binding properties of 2,10,16,24-tetrakis4-(4-hydroxyphenyl)-1-phenylethyl)phenoxy)phthalocyaninato Co(II) and Mg(II) compounds were analyzed. CT-DNA was used to determine DNA binding properties of Co(II) (4) and Mg(II) (5) phthalocyanine compounds. The DNA binding properties of compounds 4 and 5 were investigated using electronic absorption titration, fluorescence spectroscopy, cyclic voltammetry, thermal denaturation study, electrophoresis and viscosity measurements in Tris-buffer (pH 7.0). The results indicated that Co(II) and Mg(II) phthalocyanine compounds have strong affinity for CT-DNA through intercalation.
Dna Binding Studies of the 2,10,16,24-Tetrakis (phenoxy-3 Acid)phthalocyaninato) Co(Ii) and Cu(Ii) Compounds
(Springer int Publ Ag, 2020) Balli, Zekeriya; Arslantas, Ali; Solgun, Derya Gungordu; Agirtas, Mehmet Salih
Tetra phenoxy-3-methoxybenzoic acid substituted Co(II) and Cu(II) metal phthalocyanine compounds were reported for the first time. The structures of these compounds were elucidated by NMR, FT-IR, UV-vis spectroscopy, and elemental analysis. The UV/Vis, fluorescence spectra, viscosity and the thermal melting point techniques were implemented to analyze the DNA bonding activities of cobalt (II) and copper (II) complexes of phthalocyanine by calf thymus DNA. These techniques showed that the phthalocyanine complexes interacted with the DNA molecule through the intercalation mechanism. Additionally, among the methods above, the binding properties of DNA with the compounds 4 and 5 were studied by means of the agarose gel electrophoresis method. The complexes 4 and 5 bonded to the DNA molecule in the buffer system at a pH of 7.02. The findings of these methods proved that the Co(II) and Cu(II) complexes of phthalocyanine could be evaluated as potential cancer medicine for cancer treatment in the near future.
Evaluation of DNA Reactivity Properties of Zn(II) Phthalocyanine Complex Carrying Tetrakis(Benzhydryloxy) Ligand
(2025) Agırtas, Mehmet Salih; Arslantas, Ali
Using a variety of techniques, the interaction pathway of zinc(II) phthalocyanine with tetrakis(benzhydryloxy) ligand with DNA was assessed in this article. Electrophoresis, thermal denaturation, viscosity, absorption, and fluorescence spectra were employed to investigate the binding mechanism of the ZnPc complex with DNA. The DNA binding constant (Kb) of the ZnPc compound and the thermal melting profile values of the DNA showed that the binding of the ZnPc compound to CT-DNA is governed by an intercalative interaction mechanism. The Kb was computed to be 1.392 106 M-1 for the ZnPc compound, which provides very important clues about the binding mechanism. Fluorescence spectroscopy is another important tool for clarifying the ability of ZnPc to interact with DNA. The information obtained from the fluorescence method confirms that ZnPc interacts with DNA by means of intercalative binding. When the findings obtained from electrophoresis experiments were evaluated, the significant decrease in the intensity of CT-DNA bands revealed that ZnPc interacted with DNA through a physical interaction. The data from the viscosity study confirm data previously obtained by other methods. As a result of their findings, they showed that ZnPc interacts with DNA by intercalative binding. All of these informations suggest that ZnP has DNA interaction properties and could be a potential agent that can be used in the treatment of cancer diseases on the basis of its DNA bonding property.
Inquiries of Dna Interaction of Cobalt (Ii) Phthalocyanine Compound Bearing 4-Tritylphenoxy Groups
(Chem Soc Pakistan, 2024) Arslantas, Ali; Agirtas, Mehmet Salih
[Tetrakis-(4-tritylphenoxy) phthalocyaninato] Cobalt (II) (PcCo) was previously formed via the reaction of 4-(4-tritylphenoxy)phthalonitrile. The structure of the resulting PcCo was characterized using absorption spectra, infrared and NMR spectroscopies. The deoxyribonucleic acid (DNA) connecting feature for PcCo was inquired in the different concentration of Calf Thymus- deoxyribonucleic acid (CT-DNA) using UV/Vis, fluorescence spectroscopy, gel agarose electrophoresis and thermal denaturation methods. UV/Vis spectrometer and fluorescence spectroscopy verified PcCo bounds to the DNA and the binding constant (Kb) b ) of the PcCo was also calculated. The binding constant Kb b is a very important parameter for getting information on the binding mechanism. The Kb b of the compound was also calculated as 1.44 x 106 6 M-1.-1 . The value of Kb b demonstrated that the complex binds to DNA through an intercalative binding mechanism. Additionally, thermal denaturation and the electrophoresis studies were implemented to analyze the interacting of PcCo via CT-DNA. In the absence of the complex, the melting experiments were conducted for CT-DNA and Tm m was found 69.6 o C for the DNA and The Tm m of PcCo complex was found 76.3 o C. Melting point temperature and electrophoresis experiments demonstrated that PcCo binds to CT-DNA through intercalation binding mechanisms. The acquired findings confirmed that PcCo links to the DNA via the intercalating binding manner. Therefore, PcCo may have potential use in cancer treatment. Because of this, further research is needed before this complex can be used in cancer treatment.
Interaction Activity of Metal-Free Phthalocyanine Compound Bearing Tetra -(2 Accent>-Cyanoethyl)aminoethylsulfanyl) Units With DNA
(Chem Soc Pakistan, 2025) Arslantas, Ali; Agirtas, Mehmet Salih
The phthalocyanine having 4-(2-(N-2-cyanoethyl)aminoethylsulfanyl) group had been reported earlier in the literature. In this current studying, DNA binding activity of 1Pc phthalocyanine bearing 4-(2-(N-2-cyanoethyl)aminoethylsulfanyl units was examined spectroscopically via elctronic absoption, A fluorescence titration, melting point profile, A electrophoresis and viscosity methods. The interaction activity of 1Pc compound was examined at differing concentrations. UV/Vis spectrometer, viscosity, Afluorescence spectroscopy and thermal melting temperature confirmed that 1Pc binds to the DNA. The K-b of 1PcAis also estimated via UV/Vis titration and K-b of 1Pc was computed as 2.1394 x 10(6) M-1. The K(b)value demonstrated that 1Pc reacts with DNA by an intercalative mechanism. Alongside this research, the mechanism by which the compound binds to DNA was investigated by determining Tm. The Tm of DNA + 1Pc complex was identified as 74.31. This data confirmed that 1Pc binds to DNA intercalatively. All the results obtained from the used methods demonstrated that 1Pc phthalocyanine compound has an efficient DNA interaction activity and 1Pc phthalocyanine compound interacts with DNA via an intercalative mechanism. As a result, the compound mayAbe a therapeutic agent due to its DNA interaction property.
The Interaction Between a Zinc(Ii) Phthalocyanine Compound Bearing Octakis Phenoxyacetamide Substituents and Calf Thymus Dna
(Tubitak Scientific & Technological Research Council Turkey, 2018) Arslantas, Ali; Agirtas, Mehmet Salih
In this study, a zinc(II) phthalocyanine compound (PcZn) substituted with octakis phenoxyacetamide was synthesized and characterized. Calf thymus DNA (CT-DNA) was used to determine the DNA binding properties of the zinc(II) phthalocyanine compound. This interaction of CT-DNA with PcZn was investigated using electronic absorption spectra, emission spectroscopy, a thermal melting temperature study, cyclic voltammetry, and viscosity measurements in a Tris-HCl buffer solution at a pH of 7.2. The findings showed that zinc(II) phthalocyanine interacts with calf thymus DNA via partial intercalative binding mechanisms. In addition to the techniques above, a gel electrophoresis study was also carried out to verify the interaction of CT-DNA with the PcZn complex. The results demonstrated that the octakis phenoxyacetamide-substituted zinc(II) phthalocyanine complex binds to CT-DNA via a partial intercalative binding mode.
An Investigating on Dna Binding Activity of Zn(Ii) Phthalocyanine Complex Having Tetra Substituted Phenoxy-3 Acid Group
(2022) Ballı, Zekeriya; Agırtas, Mehmet Salih; Arslantas, Ali
The tetra substituted Zn(II) phthalocyanine complex having the dicyanophenoxy)-3-methoxybenzoic acid group had been obtained from 4-(3, 4-dicyanophenoxy)-3-methoxybenzoic acid and analyzed with the application of FT-IR, NMR, UV/Vis techniques in compliance withreported literature. The interacting property of Pc4 by CT-DNA was examined with absorption bands, emission titrations, melting temperature, viscosity, and gel electrophoresis procedures. The obtained findings from these techniques demonstrated that the complex containing the dicyanophenoxy)-3-methoxybenzoic acid binds to the DNA by means of intercalation attachment mechanisms.
Investigation of Dna Binding Activities of Peripherally 2,10,16,24-Tetrakis Dimethyl 5-(phenoxy) Ni(Ii) Phthalocyanine Complex
(Wiley-v C H verlag Gmbh, 2018) Arslantas, Ali; Agirtas, Mehmet Salih
Peripherally 2, 10, 16, 24-tetrakis dimethyl 5-(phenoxy)-isophthalate-substituted Ni(II) phthalocyanine compound (Pc7) was previously synthesized and characterized. In this work, calf thymus DNA was used to specify DNA binding activities of Ni(II) phthalocyanine compound. DNA binding properties of Pc7 were studied using absorption titration, fluorescense spectroscopy, thermal melting temperature study, cyclic voltametry in a Tris-HCl buffer solution. The results indicated that Pc7 can bind to CT-DNA molecule through partial intercalation binding mechanisms. In addition to above methods, agarose gel electrophoresis and viscosity measurement were also conducted to confirm DNA binding activities activities of Pc7. The results of these methods indicated that Ni(II) phthalocyanine compound adheres to CT-DNA partial intercalative binding mode. These results confirm that Ni(II) phthalocyanine compound may become potential therapeutic agent for cancerous cells due to its DNA binding features.
Investigation of Dna-Binding Activities of Zinc(Ii) and Cobalt(Ii) Phthalocyanine Compounds With 3,4,5-Trimethoxybenzyloxy Substituents
(Wiley-v C H verlag Gmbh, 2017) Arslantas, Ali; Agirtas, Mehmet Salih
In this study, DNA-binding activities of previously synthesized tetrakis(3,4,5-trimethoxybenzyloxy)-substituted Zn(II) and Co(II) phthalocyanines (Pc4 and Pc5) have been chosen for the study. The interaction of synthesized tetrakis(3,4,5-trimethoxybenzyloxy)-substituted Zn(II) and Co(II) phthalocyanine compounds with CT-DNA was investigated by using UV/Vis titrimetric method, fluorescence spectroscopy, cyclic voltammetry, thermal denaturation profile, gel electrophoresis and viscosty measurement in a Tris-HCI buffer at pH 7.0. The results indicated that tetrakis(3,4,5-trimethoxybenzyloxy)-substituted Zn(II) and Co(II) phthalocyanines (Pc4 and Pc5) can interact with CT-DNA via intercalation binding mode.
Probing the DNA Interaction Behavior of Tetrakis (Benzhydryloxy Phthalocyaninato) Mg (II) Complex
(Chem Soc Pakistan, 2025) Arslantas, Ali; Agirtas, Mehmet Salih
In this work, we evaluated the DNA interaction behaviors of previously prepared and characterized peripherally tetra-substituted Mg(II) phthalocyanine containing a tetrakis (benzhydryloxy) group. The 2, 10, 16, 24-tetrakis (benzhydryloxy phthalocyaninato) Mg(II) complex was studied is soluble in organic solvents. During the synthesis and characterization phase, absorption spectroscopy, infrared spectroscopy and 1H NMR were utilized to elucidate the chemical structure of the MgPc complex. For this complex, DNA binding behaviours were investigated with various techniques like electronic absorption spectroscopy, fluorescence emission spectra, electrophoresis and thermal denaturation. To reveal the binding pattern of the MgPc complex to DNA, the binding constant was calculated and found to be 1.73 x 10(6) M-1 for the complex. All the results obtained from the techniques applied showed that MgPc tends to interact with the DNA molecule and confirmed that this interaction occurs intercalatively.
Spectroscopic Evaluation of DNA Binding Activities of Copper (II) Phthalocyanine Complex Consisting of Tetrakis-(4-Tritylphenoxy) Ligand
(Gazi Univ, 2025) Agırtas, Mehmet Salıh; Solğun, Derya Güngördü; Arslantas, Ali
The structure and basic properties of Cu(II) phthalocyanine compound possessing tetrakis-(4-tritylphenoxy) group were elucidated in a past study with the help of absorption and infrared spectroscopic equipments. The electronic spectra, emission spectroscopy, gel agarose electrophoresis and thermal melting were employed to reveal the DNA interaction functions of this complex at changing concentrations of CT-DNA. In this experiment, the binding constant for the Cu(II) phthalocyanine compound which contains the tetrakis(4-tritylphenoxy) group was computed to be 1.53 x 106 M-1. The data obtained from absorption and fluorescence spectroscopic studies revealed that the CuPc compound reacted with CT-DNA through an intercalating mechanism. Well as the above methods, melting temperature and electrophoresis were also employed to analyse the interaction feature of CuPc with DNA. The interaction of the CuPc compound with DNA was also confirmed by data from melting temperature and electrophoresis experiments. Within the framework of the results obtained, it is predicted that CuPc compound may be a possible cancer therapeutic agent.
Studies on Dna Interaction With 2(3),9 16(17), 23(24)-Tetrakis 2((4-Phenoxyphenyl) Diazeylbenzoic Acid)phthalocyaninato Zinc (Ii) Compound
(Wiley-v C H verlag Gmbh, 2018) Arslantas, Ali; Agirtas, Mehmet Salih
In this study, 2(3), 9(10), 16(17), 23(24)-tetrakis 2((4-phenoxyphenyl) diazeylbenzoic acid)phthalocyaninato zinc (II) compound was chosen to study DNA binding activities. The binding activities of zinc (II) phthalocyanine compound bearing 2(3),9(10), 16(17), 23(24)-tetrakis 2((4-Phenoxyphenyl) diazeylbenzoic acid) ligand with Calf Thymus (CT)-DNA was investigated using absorption titration, fluorescence spectroscopy, cyclic voltammetry and thermal melting profile in a Tris-HCl buffer system at pH7.0. In addition to these methods, gel electrophoresis and viscosity measurements were also carried out to determine DNA binding activities with compound 6 in a buffer solution. The results indicated that compound 6 interacts with CT-DNA through partial intercalation binding mode.
The Study of Interaction Activity of Nickel (Ll) Phthalocyanine Complex Bearing Tetra Substituted Phenoxy-3 Acid Groups With DNA
(Gazi Univ, 2023) Agırtas, Mehmet Salih; Arslantas, Ali
Nickel phthalocyanine complex containing 3-methoxybenzoic acid groups was acquired and specified by way of Fourier Transform Infrared, NMR and UV-Visble spectroscopy procedures. Interaction of PcNi with the DNA molecule was examined via electronic absorption spectra, fluorescence spectra, melting point, viscosity, and the electrophoresis technics, respectively. The interaction activity of PcNi against the DNA was examined by way of absorption spectra titrations and the fluorescence spectra, farther by conducting melting point, viscosity procedures in the buffer of a pH 7.02. The obtained outcomes from these methods demonstrated that PcNi indicated substantial binding affinity to the DNA via intercalating by Kb of 1.31 x 106 m-1. Further, the interacting activity of PcNi on the DNA was analyzed by which electrophoresis technique and this procedure indicated that PcNi complex exhibits strong binding affinity on the DNA.
The Study on Biological Activities of Silver Nanoparticles Produced Via Green Synthesis Method Using Salvia Officinalis and Thymus Vulgaris
(Tubitak Scientific & Technological Research Council Turkey, 2022) Odemis, Omer; Ozdemir, Sadin; Gonca, Serpil; Arslantas, Ali; Agirtas, Mehmet Salih
In the present study, Ag nanoparticles (AgNPs) were synthesized from Salvia officinalis and Thymus vulgaris, known as phytotherapy plants. The obtained silver nanoparticles were characterized using SEM, XRD, FTIR, and UV/Vis spectra. The antioxidant capacities of Salvia officinalis-mediated AgNP (SO-AgNP) and Thymus vulgaris-mediated AgNP (TV-AgNP) were analyzed in vitro using 1,1-diphenyl-2-picrylhydrazyl and iron chelating activity assays. DPPH activities were 83.74% and 57.17% for SO-AgNP and TV-AgNP at concentration 200 mg/L, respectively. Both green synthesized AgNPs exhibited good iron chelating activity. In addition, the DNA cleavage activities of SO-AgNPs and TV-AgNP were investigated with agarose gel electrophoresis technique. SO-AgNPs and TV-AgNP showed single-strand DNA cleavage activity. AgNPs showed that the SO-AgNP and TV-AgNp were effective against bacteria and fungi, and antimicrobic activities were assessed as minimal inhibition concentration (MIC). Remarkably, green synthesized AgNPs showed highly effective cell viability and biofilm inhibition effect. AgNPs also demonstrated slightly antimicrobial photodynamic activity after LED irradiation.
Synthesis, Aggregation, Antioxidant and Dna-Binding Properties of Metallophthalocyanines Bearing 5-Tert Groups
(Wiley-v C H verlag Gmbh, 2017) Agirtas, Mehmet Salih; Cabir, Beyza; Ozdemir, Sadin; Okumus, Veysi; Arslantas, Ali
In this study, the synthesis and characterization of new substituted metallophthalocyanines are described. A new phthalonitrile, 4-(5-tert-butyl-2-hydroxyphenoxy)-5-chlorophthalonitrile (3) was prepared. New compounds were characterized by UV-Vis, IR, H-1 NMR, and elemental analysis. The aggregation behavior of the cobalt (II), magnesium (II) and copper (II) phthalocyanines were studied in tetrahydrofuran and in known concentration ranges. The antioxidant activities of compounds were evaluated. Their radical-scavenging capacity, metal chelating activity and reducing power was fully studied. The compound 3 showed 100% chelating activity as EDTA at concentration 50 mg/L. The DNA interaction of copper (II) phthalocyanine compound (6) was studied using UV/Vis titration, gel electrophoresis, cyclic voltammetry. The results indicated that compound 6 interacts with CT-DNA via intercalation binding mode.