Browsing by Author "Arzuk, E."

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Effect of N-Hexane Extract From Tanacetum Argenteum (Lam.) Willd. Subsp. Argenteum on the Secretion of Proinflammatory Cytokines in Thp-1 Cell Line
(Society of Pharmaceutical Sciences of Ankara (FABAD), 2024) Arzuk, E.; Karakuş, F.; Albayrak, G.; Ergüç, A.; Tan, I.; Atiş, E.
Inflammation is an initial biological process that involves the activation of the immune system in response to injury, infection or exposure to toxic agents. During this process, cytokines, small proteins produced by immune cells, play a vital role in regulating the immune response. Inflammatory cytokines, including interleukins, tumor necrosis factor-α, nitric oxide, and interferon-gamma, initiate the immune response and promote inflammation. Natural products are frequently a source of potential anti-inflammatory compounds, and screening natural products can lead to the discovery of novel bioactive compounds. The present study aimed to investigate the effects of n-hexane extract from Tanacetum argenteum subsp. argenteum on the lipopolysaccharide-induced inflammatory response in human macrophages THP-1 cell. Cells were incubated with different concentrations of n-hexane extract, and the inhibitor effects of the extract exposure on various cytokine secretions were determined. The findings demonstrated that n-hexane extract dramatically decreased the levels of interleukin-6, interleukin-1β, and tumor necrosis factor-α in differentiated THP-1 cells, indicating the remarkable anti-inflammatory potential of the extract. The n-hexane extract inhibited the secretion of interleukin-6 and interleukin-1β even at the lowest dose of 1 μg/ml. However, a significant reduction in tumor necrosis factor-α secretion was observed at 5 μg/ml and above concentrations. Importantly, the results of the study indicated that both the n-hexane extract and its active component, parthenolide, exhibit comparable effects. Furthermore, in silico analysis of toxicogenomic data revealed the interactions between the active component of the n-hexane extract and interleukin-6, interleukin- 1β, and tumor necrosis factor. © 2024 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
Isoimperatorin-Mediated Anticancer Activity: Role of Mitochondrial Dysfunction in Hepg2 Cells
(University of Ankara, 2023) Ergüç, A.; Arzuk, E.; Albayrak, G.; Karakus, F.; Okur, H.; Baykan, S.
Objective: The first goal of the present study is to investigate the role of mitochondria due to the Crabtree effect in HepG2 cells exposed to ISO in either glucose-or galactose-conditioned media. The second aim is to predict the interactions between electron transport chain (ETC) complexes and ISO, which might be the possible reason for mitochondrial dysfunction. Material and Method: Cell viability and membrane damage for HepG2 cells exposed to ISO (12.5, 25, 50, 100, and 250 uM) were assessed by MTT and LDH leakage assays in either glucose-or galactose-conditioned media. The affinity of ISO to ETC complexes was also determined by a molecular docking study. Result and Discussion: MTT assay showed that 250 uM ISO leads to cytotoxic activity in glucose-conditioned media, while 25 uM and higher concentrations of ISO decrease cell viability in galactose-conditioned media. A membrane damage assay conducted in a glucose-conditioned media assay revealed that 250 uM ISO disrupts the cell membrane. 100 and 250 uM ISO increased membrane damage in galactose-conditioned media. According to docking simulations, binding affinities of ISO to ETC complexes are in descending order: Complex IV Complex I Complex III Complex II. Inhibition of complex IV by ISO inhibits the transfer of electrons from cytochrome c to oxygen, and the proton gradient collapses. The present study proposed that ISO leads to mitochondrial dysfunction via inhibition of the ETC. © 2023 University of Ankara. All rights reserved.