Browsing by Author "Aslan, Ali"

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Agrobacterium- Mediated Transformation of Turkish Upland Rice (Oryza Sativa L.) for Dalapon Herbicide Tolerance
(Natl inst Science Communication-niscair, 2020) Kaya, Yilmaz; Aksoy, Hasan Murat; Edbeib, Mohamed Faraj; Wahab, Roswanira Abdul; Ozyigit, Ibrahim Ilker; Hamid, Azzmer Azzar Abdul; Aslan, Ali
Agrobacterium-mediated transformation of upland rice is established in few numbers of cultivars due to the high cultivar-specificity of regeneration from transformed explants. Further, dehalogenase E (dehE) gene had been characterized in Pseudomonas putida and it produces an enzyme that degrades dalapon. This study aimed to transform Turkish upland rice with the dehE herbicide resistant gene and addresses the challenges of transgenic rice recovery by identifying explant and transformation method. Constructed vector pCAMdehE carrying dehE gene was transferred into the rice shoot apex by Agrobacterium-mediated transformation. The transformed rice was analyzed for expression of the transgenes by polymerase chain reaction (PCR). Herbicide resistance leaf painting assay was carried out at different dalapon herbicide concentrations to the transgenic rice leaves. Transformation efficiency percentage (putative) was highest (32.66%) in 5 days old explants. PCR analysis resulted in the amplification of the dehE, T-DNA border endonuclease (virD2) and hygromycin phosphotransferase (hpt) genes from the transgenic rice. In addition, dehalogenase activity was proved with higher dalapon tolerance in the rice. Dalapon effects started to appear in the transformed rice at 180 mg/l, while in non-transformed ones at 60 mg/l concentration. The results showed that transformed plants have more tolerance to the herbicide than the non-transformed ones.
Anti-Mutagenic and Anti-Oxidant Potencies of Cetraria Aculeata (Schreb.) Fr., Cladonia Chlorophaea (Florke Ex Sommerf.) Spreng. and Cetrelia Olivetorum (Nyl.) W.l. Culb. & C.f. Culb.)
(Shaheed Beheshti Univ, Sch Pharmacy, 2018) Ceker, Selcuk; Orhan, Furkan; Sezen, Selma; Gulluce, Medine; Ozkan, Hakan; Aslan, Ali; Agar, Guleray
In this study, the mutagenic and anti-mutagenic effects of methanol extract of three lichen species (Cetraria aculeata, Cladonia chlorophaea and Cetrelia olivetorum) were investigated by using E. coli-WP2, Ames-Salmonella (TA1535 and TA1537) and sister chromatid exchange (SCE) test systems. The results obtained from bacterial test systems demonstrated that methanol extracts of three lichen species have strong anti-mutagenic potencies on TA1535, TA1537 strains and to a lesser extent on E. coli-WP2 strain. The anti-oxidant level of human lymphocytes cells was determined in order to clarify the mechanism underlying the anti-mutagenic effects of these lichen species. Co-treatments of 5, 10 and 20 mu g/mL concentrations of these three lichen species with AFB decreased the frequencies of SCE and the level of MDA and increased the amount of SOD, GSH and GPx which decreased by aflatoxin. The findings of this work have clearly demonstrated that Cetraria aculeata, Cladonia chlorophaea and Cetrelia olivetorum have significant anti-mutagenic effects which are thought to be partly due to the anti-oxidant activities and the interaction capability of lichen extracts with mutagen agents (Sodium azide, acridin, N-methyl-N'-nitro-N-nitrosoguanidine and aflatoxin B-1).
Cladonia Furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik Ve Oksidatif Etkileri
(2018) Emsen, Bugrahan; Kaya, Abdullah; Aslan, Ali
Mevcut çalışmada, Cladonia furcata (Huds.) Schrad. likeninden elde edilen metanol ve su ekstraktlarının (sırasıyla CME ve CSE) insan lenfositleri üzerindeki aktivitelerinin değerlendirilmesi amaçlanmıştır. Lenfositlerdeki sitotoksik etkiyi test etmek için 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür ve laktat dehidrogenaz testleri kullanılmıştır. Ayrıca, hücrelerdeki oksidatif stres ve genotoksisite değişimleri sırasıyla toplam oksidan durum ve 8-hidroksi-2?-deoksiguanozin seviyeleri ile incelenmiştir. Bu uygulamalara ek olarak, ekstraktlar ile muamele edilen lenfositlerdeki toplam antioksidan kapasite (TAK) değişimleri de belirlenmiştir. CSE'nin düşük konsantrasyonlarının yüksek seviyelerde sitotoksik etki göstermedikleri ortaya çıkarılmıştır (IC50=221,14 mg/L). Gerçekleştirilen korelasyon analizleri sitotoksisitenin oksidatif stres ile ilişkili (p < 0,01) olduğunu göstermiştir. Tüm uygulamalar göz önüne alındığında, özellikle CSE'nin negatif kontrole kıyasla hücreler üzerinde istatistiksel olarak (p > 0,05) genetik hasar meydana getirmediği tespit edilmiştir. Hücrelerdeki TAK temel alındığında, ekstraktların belli konsantrasyonlarının (12,5-50 mg/L) TAK'yi anlamlı derecede (p < 0,05) yükselttiği dikkat çekmektedir. Sonuç olarak elde edilen veriler, özellikle CSE'nin lenfositler üzerinde genotoksik ve oksidatif strese neden olmayarak, yüksek antioksidan özellikleri sayesinde yeni bir tedavi maddesi kaynağı olabileceğini ortaya çıkarmıştır
Contributions To the Lichen Flora of Turkey: New Records From Eastern Anatolia (Van Province)
(Brill, 2022) Karagoz, Yalcin; Aslan, Ali; Yazici, Kenan
Our field research in Van province during 2008-2009 resulted in collection of 3766 lichen samples from 55 sites, and identification of 229 lichen taxa, including two subspecies and three varieties, in 108 genera of Ascomycota. We found 182 new records for Van province. These include four new records for Turkey, namely Bibbya ruginosa, Buellia subalbula, Caloplaca tenuatula and Rimularia gibbosa. Lists of species and collection sites are provided. Acarospora (11 species), Lecidea (9 species), Verrucaria (8 species), Caloplaca, Physcia and Rinodina (7 species for each), and Aspicilia, Lecanora and Polyozosia (6 species for each) are the genera richest in species in the study area. Of the identified species, 163 are crustose, 43 are foliose, 7 are fruticose, 13 are squamulose and 3 are leprose. 101 species colonise on calcareous rocks, 86 on siliceous rocks, 23 on various trees (epiphytic), 10 on soil, 12 on moss and 17 on other lichens (lichenicolous). Sites 16, 19 are the richest with 55 identified taxa, followed by sites 52, 34, and 6 with 51, 49 and 44 taxa, respectively. The poorest sites are 40 and 42 with 6 identified taxa, followed by sites 5, 7, and 21 with 8, 8, and 9 taxa, respectively.
Determination of Some Antioxidant Activities and Oxidative Stress Leves in Patients With Lip and Oral Cavity Cancer
(2022) Belli, Şeyda; Ozkol, Halil; Demır, Halıt; Aslan, Ali; Mer, Ali Hussein; Demir, Canan
Squamous cell carcinomas constitute the majority of oral cavity tumors. Poor oral hygiene, smoking, chronic irritation and alcohol consumption are counted as etiological factors. In study, it was aimed to determine oxidative stress levels and some antioxidant activities such as superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) in cancer of the lip and oral cavity. Malondialdehyde (MDA) levels, SOD, GSH and CAT activities were measured using the spectrophotometric method. The results showed that SOD, CAT and GSH levels decreased significantly in the patient group (p<0.05), while MDA levels increased significantly (p<0.05). Lack of antioxidants can increase the development of oral and lip cancers.
Effects of Two Lichen Acids Isolated From Pseudevernia Furfuracea (L.) Zopf in Cultured Human Lymphocytes
(Walter de Gruyter Gmbh, 2018) Emsen, Bugrahan; Togar, Basak; Turkez, Hasan; Aslan, Ali
The present study aims at assessing the efficacies of olivetoric acid (OA) and physodic acid (PA) isolated from Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) in human lymphocytes (HLs) in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assays were performed to establish cytotoxicity in HLs. Besides, oxidative stress and genotoxicity were monitored by estimating the changes of total oxidative stress (TOS) and 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels, respectively, in HLs. At the same time, OA- and PA-induced total antioxidant capacity (TAC) levels in HLs were determined. Although especially low concentrations of OA (IC50 = 109.94 mg/L) and PA (IC50 = 665.49 mg/L) did not show cytotoxic effect at high levels in HLs, it was revealed that cytotoxicity was significantly (p < 0.05) associated with oxidative stress and genotoxicity via correlation analysis. While TOS level in HLs did not statistically (p > 0.05) increase in the presence of all treatments (0.5-100 mg/L) of PA, TAC level was increased by PA applications in certain concentrations (0.5-10 mg/L). Overall, the obtained data indicate that OA and especially PA as lichen compounds that do not cause oxidative stress can be a new resource of therapeutics as recognized in the present study with their high antioxidant features.
Effects of Use of Some Lichen Species as Plant Nutrient on Metabolic Properties and Growth Parameters of Corn Plant (Zea Mays L.)
(Taylor & Francis inc, 2024) Aslan, Ali; Emsen, Bugrahan; Susar, Ali; Kece, Yusuf Murat; Gunes, Adem
Lichens contain a sufficient amount of plant nutrients for plant growth. Studies have shown that the characteristic secondary metabolites they produce contain significant amounts of organic acids, amino acids, and hormones. This study aims to examine the effects of 10 different lichen species Aspicilia calcarea (A. calcarea), Cetraria islandica (C. islandica), Cladonia furcate (C. furcate), Flavoparmelia caperata (F. caperata), Lobaria pulmonaria (L. pulmonaria), Pseudevernia furfuracea (P. furfuracea), Rhizoplaca chrsoleuca (R. chrsoleuca), Umbilicaria vellea (U. vellaa), Xanthoparmelia somloensis (X. somloensis), Usnea longissima (U. longissima) on metabolic properties and growth parameters of maize plant for possible use as a plant food source in sustainable and organic agriculture. As a result of the research, it was observed that when lichen species were applied as plant nutrients, there were significant increases in plant height, plant weight, dry matter ratio, some nutrient content and amino acid levels of maize compared to the control group. Among the treatments, Lobaria pulmonaria application at 5 g/kg resulted in the highest values for plant height (145.00 cm), plant weight (187.00 g), and dry matter ratio (19.21%). The research shows that different lichen species significantly affect the amino acid content, nutrient content, and growth parameters of the maize plant at various levels.
Evaluation of the Biological Activities of Olivetoric Acid, a Lichen-Derived Molecule, in Human Hepatocellular Carcinoma Cells
(Springer-verlag Italia Srl, 2021) Emsen, Bugrahan; Sadi, Gokhan; Bostanci, Aykut; Gursoy, Nuray; Emsen, Ayca; Aslan, Ali
Many lichen secondary metabolites contributed to the field of pharmacology as an active ingredient of different drugs for years. In the present study, we aimed to test the anticancer activity of olivetoric acid (OA), which we isolated from Pseudevernia furfuracea (L.) Zopf in human hepatocellular carcinoma cells (HepG2). In addition, we used non-tumoral human liver cells (THLE2) to test the level of side effects of OA in vitro. For this purpose, cytotoxic (apoptotic and necrotic), oxidant, genotoxic activities and expression levels of apoptotic genes caused by different concentrations (12.5-400 mg/L) of OA were tested on both cells. Flow cytometric and cytotoxicity tests (MTT and LDH) revealed that OA (100-400 mg/L) had a higher rate of apoptotic effects on HepG2 cells compared to THLE2. Total oxidative stress and oxidative DNA damage levels caused by all concentrations of OA on HepG2 cells was significantly (p < 0.05) higher compared to negative control. Trials with concentrations of 100-400 mg/L significantly (p < 0.05) increased total antioxidant capacity on THLE2 cells compared to the control group. As a result, based on human hepatocellular carcinoma, it is hoped that OA may contribute to the combined or alternative treatment process.
Exploring of the Ameliorative Effects of Nerium (Nerium Oleander L.) Ethanolic Flower Extract in Streptozotocin Induced Diabetic Rats Via Biochemical, Histological and Molecular Aspects
(Springer, 2023) Battal, Abdulhamit; Dogan, Abdulahad; Uyar, Ahmet; Demir, Abdulbaki; Keles, oemer Faruk; Celik, Ismail; Aslan, Ali
Background Nerium oleander L. is ethnopharmacologically used for diabetes. Our aim was to investigate the ameliorative effects of ethanolic Nerium flower extract (NFE) in STZ-induced diabetic rats.Methods Seven random groups including control group, NFE group (50 mg/kg), diabetic group, glibenclamide group and NFE treated groups (25 mg/kg, 75 mg/kg, and 225 mg/kg) were composed of forty-nine rats. Blood glucose level, glycated hemoglobin (HbA1c), insulin level, liver damage parameters and lipid profile parameters were investigated. Antioxidant defense system enzyme activities and reduced glutathione (GSH) and malondialdehyde (MDA) contents and immunotoxic and neurotoxic parameters were determined in liver tissue. Additionally, the ameliorative effects of NFE were histopathologically examined in liver. mRNA levels of SLC2A2 gene encoding glucose transporter 2 protein were measured by quantitative real time PCR.Results NFE caused decrease in glucose level and HbA1c and increase in insulin and C-peptide levels. Additionally, NFE improved liver damage biomarkers and lipid profile parameters in serum. Moreover, lipid peroxidation was prevented and antioxidant enzyme activities in liver were regulated by NFE treatment. Furthermore, anti-immunotoxic and anti-neurotoxic effects of NFE were determined in liver tissue of diabetic rats. Histopathogically, significant liver damages were observed in the diabetic rats. Histopathological changes were decreased partially in the 225 mg/kg NFE treated group. SLC2A2 gene expression in liver of diabetic rats significantly reduced compared to healthy rats and NFE treatment (25 mg/kg) caused increase in gene expression.Conclusion Flower extract of Nerium plant may have an antidiabetic potential due to its high phytochemical content. [Graphics] .
Hepatoprotective Potentials of Usnea Longissima Ach. and Xanthoparmelia Somloensis (Gyelnik) Hale Extracts in Ethanol-Induced Liver Injury
(Taylor & Francis Ltd, 2025) Berkoz, Mehmet; Aslan, Ali; Yunusoglu, Oruc; Krosniak, Miroslaw; Francik, Renata
In our study, the antioxidant and anti-inflammatory effects of different lichen applications were investigated in rats using an experimental ethanol toxicity model. 48 rats were used in the study and they were divided into 6 groups with 8 rats in each group. These groups were: control, ethanol (2 g/kg), ethanol + Usnea longissima Ach. (200 mg/kg), ethanol + Usnea longissima Ach. (400 mg/kg), ethanol + Xanthoparmelia somloensis (Gyelnik) Hale (100 mg/kg) and ethanol + Xanthoparmelia somloensis (Gyelnik) Hale (200 mg/kg). The experimental work continued for 21 days. Lichen extracts and ethanol were administered by gavage to rats divided into groups. According to the experimental protocol, the experimental animals were sacrificed and their liver tissues were isolated. Biochemical parameters in serum, histological examinations, oxidative stress and inflammation parameters both at biochemical and molecular level in liver tissues were performed. Oxidative stress and inflammatory response were increased in the liver tissue of rats treated with ethanol for 21 days, and liver functions were impaired. It was found that U. longissima and X. somloensis extracts showed good antioxidant activity and conferred protective effects against ethanol-induced oxidative stress and inflammation. This could be attributed to the presence of secondary metabolites in the extract, which act as natural antioxidants and could be responsible for increasing the defence mechanisms against free radical production induced by ethanol administration.
In Vitro Evaluation of Cytotoxic, Oxidative, Genotoxic, and Apoptotic Activities of Physodic Acid From Pseudevernia Furfuracea in Hepg2 and Thle2 Cells
(Taylor & Francis Ltd, 2021) Emsen, Bugrahan; Sadi, Gokhan; Bostanci, Aykut; Aslan, Ali
In the present study, cytotoxic (apoptotic and necrotic), antioxidant, prooxidant, genotoxic, and apoptosis-related genes expression modulatory effects of physodic acid (PA) isolated from Pseudevernia furfuracea on cancerous (HepG2) and healthy (THLE2) human liver cells were evaluated. Cytotoxic effects PA obtained from P. furfuracea on HepG2 and THLE2 cells were tested via MTT and LDH analyses. In order to determine genotoxic effect of PA, 8-OH-dG levels in the cells were measured. In addition, apoptotic or necrotic effects of PA on the hepatic cells were determined by qRT-PCR analysis. Cytotoxicity analyses revealed the cytotoxic effect of PA on HepG2 cells, which is much higher than THLE2 cells. Gene expression studies demonstrated the apoptotic effects of PA on HepG2 cells through both extrinsic and intrinsic pathways. PA increased the antioxidant capacity of THLE2 cells with increasing concentrations, which describes its antioxidant power. Additionally, none of the tested levels of PA caused oxidative DNA damage on THLE2 cells, but its high concentration increased genetic damages on HepG2. Taken together, P. furfuracea lichen and its metabolite, PA, would be a promising alternative for complementary treatments against liver cancer.
Investigation of Antiepileptic Potentials of Usnic Acid and Some Lichen Species on the Behavioral and Biochemical Levels in Pentylenetetrazole-Induced Kindling Model of Epilepsy
(Marmara Univ, 2024) Berkoz, Mehmet; Yunusoglu, Oruc; Aslan, Ali; Bozkurt, Ayse
In this study, the effects of various lichen and usnic acid applications on seizure scores and biochemical parameters in brain tissue in rats with epilepsy model was investigated. For this aim, 91 rats were divided into 13 groups, each containing 7 rats, which were: control, pentylenetetrazole (PTZ) (35 mg/kg), PTZ + Valproic acid (100 mg/kg), PTZ + Dolichousnea longissima(200 mg/kg), PTZ + Dolichousnea longissima (400 mg/kg), PTZ + Xanthoparmelia somloensis( 50 mg/kg), PTZ + Xanthoparmelia somloensis(200 mg/kg), PTZ + Cetraria islandica(250 mg/kg), PTZ + Cetraria islandica(500 mg/kg), PTZ + Pseudevernia furfuracea(250 mg/kg), PTZ + Pseudevernia furfuracea(500 mg/kg), PTZ + usnic acid (50 mg/kg), and PTZ + usnic acid (200 mg/kg). All items were applied with an interval of 120 minutes for a period of one week. Seizure detection, seizure scores and total seizure duration of each group was recorded. After the applications, oxidative stress parameters and acetylcholinesterase enzyme activity in the brain tissue of rats were measured. There was no difference between the groups in the 1st, 2nd, and 3rdinjections (p>0.05). Starting from the 4th injection, the seizure score was significantly higher in the PTZ group compared to the control group (p<0.05). When the effects on locomotor activity were evaluated, no difference was found between any group (p>0.05). In PTZ applied groups, an increase in lipid and protein oxidation as well as a decrease in antioxidant and acetylcholine esterase levels were observed(p<0.05). Valproic acid, high concentration of lichen extract applications and high and low concentration of usnic acid applications were found to reverse this situation (p<0.05). As a result, various lichen extracts and usnic acid were shown to reduce behavioral symptoms and oxidative stress of epilepsy, with a preventive effect on the complications of epilepsy.
Investigation of Antimicrobial and Antioxidant Activities of Some Lichens
(2023) Us, Dilek; Kara, Ayşe Aydan; Bulbul, Leman Elif; Akıncıoğlu, Hülya; Aslan, Ali; Taskin, Mesut
This study investigated the antimicrobial and antioxidant properties of methanol extracts from five lichen species: Polycauliona candelaria (Syn. Xanthoria candelaria), Nephromopsis chlorophylla (Syn. Cetraria chlorophylla), Circinaria calcarea (Syn. Aspicilia calcarea), Bryoria capillaris (Syn. Alectoria cana), and Peltigera canina (Syn. Dermatodea canina). Antimicrobial activities were determined using the agar disc diffusion method, while minimal inhibitory concentration (MIC) values were determined using the micro-well dilution method. Among the lichen species, methanol extract of Circinaria calcarea exhibited the highest antimicrobial activity, with a 15 mm zone of inhibition against Escherichia coli and Bacillus cereus. It also demonstrated the lowest MIC value (31.25 μg/mL) against Bacillus cereus, Bacillus subtilis, Clostridium perfringens and Escherichia coli were the most sensitive microorganisms to lichens. Various antioxidant determination methods were employed to assess the antioxidant activities of the lichens, including ferric ion (Fe+3) reduction capacity, cupric ion (Cu+2) reduction capacity, ferric ion reducing antioxidant power (FRAP), DPPH radical scavenging activity, DMPD radical scavenging activity and metal chelating activity using the bipyridyl reagent. All lichens exhibited excellent antioxidant activity, particularly in metal chelating activity using the bipyridyl reagent. Peltigera canina demonstrated the highest antioxidant activity among the studied lichen species across most of the applied method.
Likenlerin Doğal İnsektisit Olarak Kullanımı
(2018) Emsen, Bugrahan; Aslan, Ali
Tarım, geçmişten günümüze kadar insanoğlu için en büyük istihdam kaynaklarından biri olmuştur. Bu alanda elde edilen ürünler ülke ekonomilerine önemli ölçüde katkı sağlamaktadır. Bununla birlikte, ürün veriminde düşüşlere sebep olan organizma sayısı bir hayli fazladır. Zararlı organizmalar ile mücadele konusunda tercih edilen uygulamaların başında kimyasal insektisit kullanımı gelmektedir. Fakat bu insektisitlerin yan etkilerinin yüksek düzeyde olması araştırmacıları alternatif çalışma konularına yönlendirmiştir. Biyolojik mücadele başlığı altında ele alınan, doğal ürünler aracılığı ile insektisit üretimi popüler alanlar içerisinde yer almaktadır. Likenler de yapılarında bulunan kendilerine özgü bileşenleri sayesinde doğal insektisit kaynaklarına malzeme olmaktadır. Yıllardır birçok alanda yararlanılan likenlerin gerek ekstraktları gerekse saf bileşenleri aracılığı ile farklı böcek türleri üzerinde toksik etkileri test edilmektedir. Bu noktada, likenlerden yüksek oranda verim elde edilmekle beraber çalışmaların ağırlıklı olarak ekstraktlar üzerinde gerçekleştirildiği dikkat çekmektedir. Tüm bu çalışmalar ışığında, farklı türler üzerinde insektisit aktivite gösteren likenlerdeki aktif bileşenlerin ve böceklerdeki etki mekanizmalarının tespiti üzerine gerçekleştirilecek çalışmaların artırılması ile biyolojik insektisit üretim basamağına katkı sağlanacaktır.
The Metal Complexes of Natural Compounds, Usnic Acid and Naringenin Immobilized on Mesoporous Silica: Solid Phase Extraction and Oxidative Catalytic Properties
(Chem Soc Pakistan, 2021) Urus, Serhan; Cakir, Ahmet; Ulucay, Ibrahim Ethem; Aslan, Ali
The novel semi-natural Schiff bases were synthesized with the reaction of the natural compounds usnic acid and naringenin extracted and purified in natural sources and chemically modified silica gel. Cu(II), Ni(II), Co(II) and Mn(II) complexes of the ligands were synthesized and the silica-supported ligands and their metal complexes were characterized by FT-IR, TG/DTA, SEM, EDX, ICP-OES and elemental analyses techniques. The solid-phase extraction behaviours of the novel silica-supported ligands were examined. It was found that the hybrid materials showed the very efficient adsorption properties to Cu(II), Cd(II), Pb(II) and Cr(III) metal ions maximum at pH=8-9. The metal ions could be recovered again nearly 100 % from the silica-supported ligands in the recycling experiments. The catalytic efficiencies of the metal complexes in the oxidation reactions of cyclohexane, cylohexene, cyclooctane and styrene subtrates were examined under microwave irradiation in the present of H2O2. The complexes showed very good catalytic activities in the oxidation reactions of the various tested substrates.
Prophylactic Effect of Myricetin and Apigenin Against Lipopolysaccharide-Induced Acute Liver Injury
(Springer, 2021) Berkoz, Mehmet; Unal, Seda; Karayakar, Fahri; Yunusoglu, Oruc; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Aslan, Ali
Background Liver has an important role in the initiation and progression of multiple organ failure that occurs in sepsis. Many natural active substances can be used to reduce the liver injury caused by sepsis. For this aim, the effects of myricetin and apigenin on mice model of acute liver injury was evaluated in this study. Methods and results Thirty-six mice were randomly divided into six groups as; control, lipopolysaccharide (LPS) (5 mg/kg), LPS + myricetin (100 mg/kg), LPS + myricetin (200 mg/kg), LPS + apigenin (100 mg/kg), and LPS + apigenin (200 mg/kg) groups. Myricetin and apigenin were administered orally for 7 days, and LPS was administered intraperitoneally only on the 7th day of the study. 24 h after LPS application, all animals were sacrificed and serum biochemical parameters, histopathology and oxidative stress and inflammation markers of liver tissue were examined. Myricetin and apigenin pre-treatments increased serum albumin and total protein levels, liver GSH level and catalase and SOD activities and decreased serum ALT, AST, ALP, gamma-GT, CRP, total and direct bilirubin levels, liver MPO activity, MDA, NOx, PGE(2), TNF-alpha, IL-1 beta, and IL-6 levels, iNOS and COX-2 mRNA levels, phosphorylation of NF-kappa B p65, I kappa B, and IKK proteins but not p38, ERK, and JNK proteins in LPS-treated mice. Myricetin and apigenin administration also regained the hepatic architecture disrupted during LPS application. Conclusion Myricetin and apigenin pre-treatments led to reduction of liver injury indices and oxidative stress and inflammatory events and these flavonoids has probably hepatoprotective effects in acute liver injury.
Pseudevernia Furfuracea (L.) Zopf ve Sekonder Metabolitlerinin Hepatosellüler Karsinom Hücreleri Üzerindeki Antioksidan Potansiyeli: Antioksidan Enzimlerin Düzenlenmesi
(2021) Emsen, Bugrahan; Gürsoy, Nuray; Aslan, Ali; Sadi, Gökhan
Dünyada kansere bağlı ölümlerin önde gelen nedenlerinden biri olan hepatosellüler karsinom (HCC) gibi çok sayıda hastalığın tedavisinde tıbbi bitkilerin ve sekonder metabolitlerin kullanımı artmaktadır. Oksidatif stres, karaciğer kanseri gelişimine katkıda bulunur ve antioksidan sistemlerin teşvik edilmesi, tedavi için daha iyi bilgiler sağlayabilir. Bu çalışmada, Pseudevernia furfuracea (L.) Zopf likeninin antioksidan potansiyeli araştırılıştır. Ayrıca, P.furfuracea'dan izole edilen ana sekonder metabolitler olan olivetorik asit (OA) ve fisodik asitin (PA) hepatik antioksidan enzimlerin gen ekspresyonları üzerindeki etkileri hem kanserli (HepG2) hem de sağlıklı (THLE2) insan karaciğer hücrelerinde değerlendirilmiştir. Sonuçlara göre, P. furfuracea'nın toplam fenolik içeriği etanolik ve sulu ekstraktlar için sırasıyla 71.52 µg/mg ve 8.16 µg/mg gallik asit eşdeğeri bulunmuştur. Aynı şekilde β-karoten ve likopen içerikleri de etanolik ekstraktlarda daha yüksek olarak belirlenmiştir. Bu antioksidan bileşenlerle uyumlu olarak, etanol özütünün DPPH (2,2-difenil-1-pikrilhidrazil) radikal süpürme aktivitesi (IC50: 158.79 mg/L), sulu ekstraktlar (IC50: 630.33 mg/L) ile karşılaştırıldığında oldukça yüksektir. OA ve PA, HepG2 hücrelerinde tüm antioksidan enzimlerin gen ifade düzeylerini arttırırken, sağlıklı THLE2 hücrelerinde ise sadece gpx ekspresyonu yukarı yönde regüle olmuştur. Bundan farklı olarak, her iki metabolit de THLE2 hücrelerinde cat, sod1 ve sod2 ekspresyonlarını baskılamıştır. Bu sonuçlar P. furfuracea'nın sadece yüksek antioksidan potansiyeline sahip olmadığını, aynı zamanda sekonder metabolitlerinin de karaciğer kanserinde onkogenezi ve tümör ilerlemesini önleyecek antioksidan enzimleri yukarı regüle ederek kanser hücrelerinde oksidatif stresi azaltabileceğini göstermektedir.
Records of Lichenicolous Fungi New for Turkey and Asia
(Univ Belgrade, inst Botany & Botanical Garden, 2019) Yazici, Kenan; Etayo, Javier; Aslan, Ali; Karahan, Dilara
Three lichenicolous fungi occcuring on Tephromela atra and Lecidea fuscoatra-Rhymbocarpus fuscoatrae, Sclerococcum tephromelarum and Skyttea tephromelarum, collected from the Tunceli and Bingol provinces of Turkey - are reported as new to Turkey and Asia, following a recent lichenological survey in those provinces. Short descriptions are provided, together with geographic distributions, hosts, and comparisons with similar taxa.
Rosa Canina L. Ethanolic Extract Induces the Anti-Proliferative and Apoptosis Potential in Mcf-7 and Mda-Mb Cell Lines
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Yildirim, Metin; Allahverdiyev, Oruc; Aslan, Ali
Rosa canina L. (rose hip) fruits have been used for their diuretic, laxative, anti-gout, anti rheumatism properties in traditional medicine. Rose hip berries contain a variety of components such as flavonoid. The previous studies showed that flavonoid has anti-cancer properties. Tha aim of this study is to evaluate and screen the effect of apoptosis and the anticancer potential of rose hip ethanolic extract on human breast cancer cell lines; MCF-7 and MDA-MB-468. The anti-proliferative activity of rose hip extract was evaluated using MTT, flowcytometry by annexin V/PI double staining, and caspase-3 activity. The results of MTT showed that the EDso of both human breast cancer cell lines was 25 g/mL of rose hip extract, 48 hours after treatment. Flowcytometry by annexin V/PI showed that rose hip extract induced late apoptosis in MCF7 and early apoptosis in MDA-MB-468. In addition, the caspase-3 colorimetric method showed that caspase-3 increased in the MDA-MB-468 after treatment with rose hip extract. As a result, the ethanol of rose hip ethanolic extract induced apoptosis in both human breast carcinoma cell lines.
Synergistic Gastroprotective and Antioxidative Effects of Natural Olive Oil and Usnic Acid Isolated From Usnea Longissima, a Lichen Species in Anatolia (Türkiye), in the Indomethacin Ulcer Model Created in Rats
(2023) Odabasoglu, Fehmi; Uyanık, Serkan; Dumlu, Fadime Atalay; Berktas, Ozlem Aydın; Aslan, Ali
Usnea longissima, a medically important lichen growing up in forests in Anatolia (Turkey). In this study, the gastroprotective effect of usnic acid (UA) was investigated using an indomethacin (IND)-induced gastric ulcer model in rats. While 25, 50, 100 and 200 mg/kg UA doses were dissolved in 2 ml of olive oil (OO) and administered to rats, only OO was given to one group. In addition, lansoprazole (LAN) and ranitidine (RAN) and IND were dissolved in water and administered to rat groups. IND administration caused very high levels of damage to rat stomachs. On the other hand, when four doses of UA, OO, RAN and LAN were administered, it was determined that hyperemias in the stomach of rats was significantly reduced. After macroscopic analysis of gastric tissues, the activities of superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO) and nitric oxide synthase (iNOS and cNOS) enzymes as well as glutathione (GSH) and lipid peroxidation (LPO) levels were determined in these tissues. After IND application, it was detected increases in MPO, CAT and iNOS activities in gastric tissues and decreases in SOD, cNOS and GSH amounts. Four doses of UA, OO, RAN and LAN applications reversed the trend, bringing them closer to healthy levels.