Browsing by Author "Aslan, R"

Now showing 1 - 4 of 4

Acute Phase Effect of Trichloroethylene Ingestion on Some Biological Markers in Dogs
(Scientific Technical Research Council Turkey, 2000) Aslan, R; Tütüncü, M; Aslan, SO; Dündar, Y
Trichloroethylene (TE) is an environmental toxic solvent hazardous to human and domestic animals and well known in the industrial sector. The purpose of this study was to determine whether oral TE plays a role in lipid oxidation and tissue damage. Fourteen dogs were treated with an oral toxic dose of 0.5 ml/kg T.E. The acute changes that occurred in creatine kimase (CK), malondialdehyte (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were investigated in blood samples taken before application and 8, 24, 48, 72 and 168 hours after treatment. SOD and GSH-Px activities decreased, protein, albumin and alcalen phosphate did not change and the others increased a and 24 h after the TE treatment. Forty-eight hours after the treatment, MDA, CK, albumin and AST increased, while SOD and GSH-Px decreased. Ail markers returned to their normal levels after 72 h. It was concluded that TE plays a role in oxidative stress and tissue damage in the acute phase, Repeated intake of oral TE can reach serious toxicity in domestic animals living around industial zones polluted with chemicals.
Blood Lipoperoxidation and Antioxidant Enzymes in Healthy Individuals: Relation To Age, Sex, Habits, Life Style and Environment
(Marcel dekker inc, 1997) Aslan, R; Sekeroglu, MR; Gultekin, F; Bayiroglu, F
We report the concentrations of superoxide dismutase (SOD), glutathione peroxidase (GSN-Px) and malonaldialdehyde (MDA) an end product of lipoperoxidation in human whole blood. Method for measuring was the based on spectrophotometer. Blood MDA level was higher in elderly than in children, adults. Smoking, alcohol taking, acute exercise and air pollution group's MDA levels were increased than their control groups. GSH-Px activity was significantly lower in acute exercise group, and higher in trained group than those of controls. SOD was decreased in elderly, smoking and acute exercise groups and increase in trained individuals. As a result the mean values we obtained were slightly significant in age groups. The life style, environment has polluted air and habits are thought to be responsible for these significance also.
Investigation of Malondialdehyde Formation and Antioxidant Enzyme Activity in Stored Blood
(Vsp Bv, 1997) Aslan, R; Sekeroglu, MR; Tarakcioglu, M; Koylu, H
In the present study, fresh blood obtained from six healthy adult male donors was investigated for erythrocyte malondialdehyde (MDA) formation and antioxidative enzyme activity. Plasma and erythrocyte membrane lipid peroxidation were measured by MDA formation. Erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined in whole blood stored for transfusion purposes. Erythrocyte and plasma MDA levels increased significantly during the storage period from day 3 to day 19 and, after that, stayed unchanged. Erythrocyte MDA increased 100% on day 7 when compared to day 1. The GSH-Px activity significantly decreased after day 9 and SOD decreased after day 13. The reduction in enzyme activities continued until day 27. Our results showed significant alteration in erythrocyte membrane and plasma MDA formation and intracellular antioxidant enzyme status in whole blood used for transfusion. However, we do not know whether such alterations have clinical importances for the recipient.
Malondialdehyde Levels in Serum of Patients With Skin Cancer
(Springer, 1998) Bekerecioglu, M; Aslan, R; Ugras, S; Kutluhan, A; Sekeroglu, R; Akpolat, N; Karakok, M
The relationship of free radicals with some types of carcinomas is known, these studies have been performed on samples taken from the tumor tissue. In this study, the malondialdehyde levels in the serum of patients with skin cancer were studied and compared with a control group. The differences between malondialdehyde levels in these two groups were evaluated using Mann Whitney-U. There was a very statistically significant increase in malondialdehyde levels in serum of the skin cancer patients compared with the control group (p=0.0095, z=-2.59). Thus, increased serum malondialdehyde levels may be a sign of carcinoma.