Browsing by Author "Atalan, E"

Now showing 1 - 7 of 7

The Characterization of Protein Profiles of the Isolated Aeromonas Sobria Strains From Animal Faeces by Sds-Page
(indian veterinary Journal, 2004) Boynukara, B; Korkoca, H; Senler, NG; Gulhan, T; Atalan, E
Aeromonas spp. are members of the Vibrionaceae (Janda, 1991). Although they are common in fresh surface water, their presence in faeces of several animals and humans has been proved (Boynukara et al., 2002; Jindal et al., 1993). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins have been used for typing several bacterial species, including Aeromonads (Stephenson and Tabaqchali 1986; Stephenson et al., 1987). A more sensible approach might be to identify isolates as A. hydrophila, A. sobria or A. caviae, and use an electrophoretic fingerprinting technique to further characterize them (Popoff, 1984), Maclnnes et al. (1979) did the first DNA hybridization experiments with Aeromonas, and concluded that the genus consist of two main evolutionary lines; a diverse group of motile Aeromonads and genetically more homogeneous non-motile Aeromonads. Fanning et al. (1985) confirmed that all strains of Aeromonas were more closely related to each other than to species in other families, and found that motile Aeromonas species could be divided into at least 10 different DNA hybridization. In this study, we aimed to characterize the Aeromonas sobria isolated from Turkish Van cat, chicken, gull and Rainbow trout and identified according to Popoff (1984) using SDS-PAGE method.
Characterization of Staphylococcus Species by Sds-Page of Whole-Cell and Extracellular Proteins
(Maik Nauka/interperiodica, 2003) Berber, I; Cokmus, C; Atalan, E
In this study, a total of fifteen staphylococcal strains belonging to different species were characterized by whole-cell and extracellular protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results are presented as dendrograms after quantitative analysis of the band patterns with a computer program. Visual inspection of protein bands and cluster analysis of protein patterns of to be used 15 strains, representing 10 Staphylococcus species, showed that whole-cell and extracellular protein profiles differed in several protein bands in Staphylacoccus aureus, S. epidermidis, S. simulans, and other species of Staphylococcus; however, the differences were insufficient for reliable differentiation of Staphylococcus species by the SDS-PAGE method.
Diversity of Microfungi in Soil Polluted by Cement Factory
(Parlar Scientific Publications (p S P), 2005) Biyik, H; Imali, A; Atalan, E; Tüfenkci, S; Ögün, E
The aim of this study was to investigate the microfungi flora of polluted soils of a cement factory in Edremit, southwest of Van, Turkey. Soil samples were collected from 10 different locations, from 100 meter to 5 km from the factory. The fungi were isolated on rose-bengal-streptomycin agar plates using dilution-plate technique. The number of microfungi ranged from 1 x 10(5) to 5.35 x 10(5) cfu. Isolated genera and species were Penicillium (P. decumbens, P. funiculosum, P. glabrum P. gladioli, P. soppii, P. thomii, P. turbatum), Aspergillus (A. alliaceus, A. candidus, A. flavus, A. niger, A. ochraceus), Mycelia sterilia (3 different types), Fusarium (F. oxysporum), Cladosporium (C tenuissimum), Nannizzia (N. grubyia), Doratomyces (D. microsporus), Paecilomyces (P. byssochlamydoides), Cylindrocarpon (C candidum), Beauveria (B. bassiana), Rhizopus (R. stolonifer) and Minimedusa (M. polyspora). Species composition varied in different soil samples. Total microfungi number and frequency of some microfungi species may be affected by pollution. It seems that there is a slight correlation between the microfungi number and some heavy metal concentration such as iron. Also, a slight relation between organic matter (r = 0.544), CaCO3 (r = 0.463), nickel (Ni2+) (r = 0.617) and microfungi count was found, but an adverse relation to available phosphorus (r = -0.486), exchangeable potassium content (r = -0.468) and moisture content (r = -0.401). Moreover, there are very significant correlations between the following characters: Organic matter-pH, Mn-pH, Pb-salty, Fe-organic matter, and lower significant correlations following these: Mn-salty, P-Cr, Na-organic matter, Pb-Na, Cd-moisture, K-Cr, P-Ni, Pb-Cu and Mn-P.
Effect of Gramoxone® Herbicide on Spore Viability and Larvicidal Activity of Bacillus Sphaericus 2362 and 1593 Strains
(Parlar Scientific Publications (p S P), 2003) Berber, I; Atalan, E; Çokmus, C
Spore viability and larvacidal activity of B. sphaericus 2362 and 1593 strains were monitored in media containing Gramaxone(R) herbicide. Minimal Inhibitory Concentrations (MICs) of the herbicide were determined for each of the mosquito pathogenic strains. Addition of paraquat into the media suppressed the spore germination after 24 and 48 h, but no influence was observed on parasporal inclusions containing insecticidal toxin proteins. SDS-PAGE analysis confirmed the presence of the so-called binary toxin comprising two proteins that are deposited in parasporal crystals treated with the herbicide. Moreover, the number of heat-resistant spores and total cell numbers of test strains were reduced drastically when test strains were grown in media containing 50, 100 and 200 mg ml(-1) of paraquat. In contrast, there were no differences between heat-resistant spore counts of both strains when grown under diet concentrations of less than 50 mg ml-1. This study revealed that B. sphaericus 2362 strain has a higher mosquito larvacidal activity than 1593 strain in the presence of paraquat in the media. Thus, the use of Gramaxone(R) herbicide can have harmful effects on mosquitocidal biological control agents.
The Identification of Streptomyces Isolated From Environmental Soil Samples With Hypal Mycelium Inoculum on Computer-Assisted Identification
(Marcel dekker inc, 1994) Atalan, E
Representatives of Streptomyces strains isolated from environmental soil samples were subjected to identify for the diagnostic characters recommended by Williams el of. (1983b) as a duplicate. Diagnostic characters were tested using a mixture of spores and mycelial fragments, respectively, in order to identify some Streptomyces species and strains on computer assited identification. Most of the marker strains were identified to tile correct clusters. In Contrast, 20 strains out of 38 unknown strains were assigned to any major cluster described by Williams et al. (1983a). Computer-assisted identification procedure has provided reliable identification scores for marker or unknown strains.
The Influence of Pesticides on the Spore Viability, Toxin Stability and Larvicidal Activity of Bacillus Sphaericus 2362 Strain
(Parlar Scientific Publications (p S P), 2004) Berber, I; Atalan, E; Cokmus, C
In this study, 12 different commercial pesticides, including 5 fungicides, 5 herbicides, 1 aphicide and 1 acaricide, were investigated for their effects on spore viability, toxin stability and larvicidal activity of a mosquito pathogenic B. sphaericus 2362 strain. Minimal inhibitory concentrations (MICs) of each pesticide were determined after 48 h incubation of B. sphaericus 2362 strain. According to MIC results, all pesticides showed various inhibitory effects on the growth of the test bacterium. Particularly, the pesticides which contain TCA, hymexazol, copper sulphate, hexythiazox, 2,4-D Amine, copper inhibited spore germination significantly and bacterial growth even at the lowest tested concentrations. Our results indicated that various commercial pesticides in different concentrations reduced the spore viability significantly and heat-resistant spore numbers of B. sphaericus 2362 strain. In general, the pesticides decreased the spore numbers 10(4)-10(5)-fold compared to initial ones. Furthermore, it was observed that the larvicidal activity was completely lost when it was grown at the concentration of MIC values or higher. Indeed, SDS-PAGE analysis confirmed that the binary toxin proteins, 41.9 kDa and 51.4 kDa, were lost at the MIC values and higher concentrations although parasporal crystals did not disappear in the much lower pesticide concentration.
Some Pollution Parameters in Water Samples From Lake Van, Turkey
(Parlar Scientific Publications (p S P), 2005) Ögün, E; Atalan, E; Özdemir, K
The east beach strip of Lake Van is heavily populated and the place of wastewater discharges from a municipal refinery plant. To assess both the pollution rate and heterotrophic bacterial population in the lake water, samples were collected from 8 selected stations, 10 and 100 m far from the coast between the towns of Edremit and Zeve (approximately 20 km along the coast). Physico-chemical features (pH, temperature, dissolved 0,, biochemical 0, demand, suspended solid matter, total solid matter) of the lake have also been measured. Water samples were inoculated on starch casein agar supplemented with cycloheximide (50 mu g/ml) and nystatine (50 mu g/ml) to isolate actinomycetes, whereas nutrient agar and plate count agar technique were used to isolate the other microorganisms. The physico-chemical character of different water samples varied considerably. No actinomycetes were observed, but the total number of microorganisms was recorded to be 10(5) CFU ml(-1) in some water samples. The most diverse and active bacterial populations were found in the samples collected from the outlet of a wastewater treatment plant. Although a huge rate of organic matter flows into the lake, the water samples are polluted to a lesser degree owing to high soda content and high pH. Overall, the prokaryotic communities in the highly alkaline Lake Van represent an interesting new example of gram +/- bacteria well-adopted to a broad salinity range. But precaution should be taken into consideration as soon as possible to protect the east beach strip of Lake Van.