Browsing by Author "Atalan, E."

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Antibacterial Activity of Some 4-Pyridinone Derivatives Synthesized From 4-Pyrones
(Asian Journal of Chemistry, 2008) Atalan, E.; Biyik, Halil; Batal, H.; Ozdemir, K.
Antibacterial activity of total 11newly synthesized compounds of 4-pyrone and 4-pyridinone derivatives were determined against standard bacterial test microorganisms by the tube dilution technique. Most of the compounds, except compound 11, exhibited antimicrobial activity towards the gram-negative bacterium Escherichia coli, while other compounds inhibited either 1 or 2 or 3 the growth of test microorganisms. Antibacterial activities of the compounds were exhibited strong activity and some were moderate activity while none of compounds did not inhibit the growth of S. aureus, S. pyogenes, E. facealis and B. subtilis.
Antibacterial Activity of Some 4-Pyridinone Derivatives Synthesized From 4-Pyrones (Vol 20, Pg 25, 2008)
(Asian Journal of Chemistry, 2008) Atalan, E.; Biyik, Halil; Battal, H.; Ozdemir, K.
Biosystematic Studies on Novel Streptomycetes From Soil
(2000) Atalan, E.; Manfio, G.P.; Ward, A.C.; Kroppenstedt, R.M.; Goodfellow, M.
Members of three putatively novel Streptomyces species, designated Streptomyces groups A, B and C, were repeatedly isolated from environmental samples taken from four hay meadow plots at Cockle Park Experimental Farm, Northumberland (UK). Representative isolates were found to have properties consistent with their classification in the genus Streptomyces and were recovered in three taxa using different phenotypic criteria, namely morphological and pigmentation properties, rapid enzyme tests, and whole-organism fatty acid, protein electro-phoretic and pyrolysis mass-spectrometric data. The isolates were rapidly characterised as three taxonomic groups using pyrolysis mass spectrometry. The three taxa were also distinguished from one another and from validly described species of Streptomyces using rapid enzyme tests based on the fluorophores 7-amino-methylcoumarin and 4-thylumbelliferone, and computer-assisted identification procedures. The results indicate that selective isolation and rapid characterisation of streptomycetes using pyrolysis mass spectrometry provide a practical way of determining the phenotypic species diversity of streptomycetes in natural habitats. The experimental data also indicate that representative sampling of cultivable streptomycetes from soil can best be achieved using a multi-step extraction procedure coupled with the use of selective isolation procedures.
Chemotaxonomic and Molecular Methods Applied To the Polyphasic Classification of Streptomyces Strains
(1995) Atalan, E.
Whole-organism protein of 9 test strains and fatty acid profiles for 12 test strains were analyzed for polyphasic taxonomy of strains isolated from soil samples. Representatives of test strains belonging to the same group were clustered into discrete groups after performing numerical analysis of the whole-organism protein profiles using the Pearson product moment correlation coefficient, and unweighted pair groups by using the arithmetic averages algorithm. Also, the test strains in same group were recovered in three well-separated clusters when the fatty acid profiles were analyzed quantitatively, or rather, by using Euclidean distances of the fatty acids with unweighted pair group method with arithmetic averages algorithm.
Classification of Novel Soil Streptomycetes as Streptomyces Aureus Sp. Nov., Streptomyces Laceyi Sp. Nov. and Streptomyces Sanglieri Sp. Nov
(2003) Manfio, G.P.; Atalan, E.; Zakrzewska-Czerwinska, J.; Mordarski, M.; Rodríguez, C.; Collins, M.D.; Goodfellow, M.
The taxonomic positions of soil isolates known as Streptomyces groups A, B and C were clarified. Comparative 16S rDNA sequence studies indicated that representatives of all three taxa formed distinct phyletic lines within the Streptomyces tree though the group A strains were shown to be related to Streptomyces griseus and associated validly described species. The taxonomic integrity of all three groups was highlighted by DNA:DNA relatedness and ribotype data though the group A strains encompassed a higher degree of genetic variation than the group B and C strains. In light of these and earlier phenotypic data it is proposed that Streptomyces groups A, B and C be given species status as Streptomyces sanglieri sp. nov., Streptomyces aureus sp. nov. and Streptomyces laceyi sp. nov., respectively.
Effect of Pesticides on the Growth of Bacillus Thuringiensis Var. Israelensis and Bacillus Sphaericus 2362 Strain
(2003) Berber, I.; Atalan, E.; Cokmus, C.
In this study, twenty-three different commercial pesticides including nine insecticides, six herbicides, four fungicides, three plant growth regulators and one acaricide were investigated for their effects spore viability and larvicidal activity of mosquito pathogenic Bacillus thuringiensis var. israeletisis and Bacillus sphaericus 2362 strain. Minimal inhibitor concentration (MIC) for each pesticide was determined for test bacteria grown into nutrient yeast salt (NYSM) broth. All pesticides except four had an adverse impact on bacterial growth, according to the MIC values. The most inhibitory insecticide, herbicide, fungicide and plant growth regulator were endosulfan 62.5 μL mL-1, diclofop-methyl 28 EC 62.5 μ mL-1, copper sulphate 12.5 μg mL-1 and gibberellic acid 0.25 mL mL-1, respectively. Neoron 500 EC, an acaricide, 31.2 μ mL-1 was the most inhibiting of all pesticides. In general, there was no significant difference between the susceptibility of Bacillus thutbigiensis var. israelensis and Bacillus sphaericus 2362 strain for pesticides examined in this study. High concentrations of all pesticides reduced heat-tolerant spore samples by suppressed spore germination. No larvacidal activity was observed at the MIC values or higher. These results should be considered for assessing the MIC value of these pesticides when both microbial pest agents used in field application. © International Scientific Publications, New Delhi.
Identification of Biodiversity of Some Streptomyces Species and Determination of a Restriction Fragment Length Polymorphism (Rflp) Profile of 16s Rdna Gene Region
(Medwell Journals, 2014) Ozdemir, K.; Ogun, E.; Ertas, M.; Acar, S.; Atalan, E.
In this study, soil samples from the roots and peripheral regions of some agricultural plant species growing in the Van region (such as sainfoin, bean, malus, wheat and sugar beet) were collected between May and June 2004 and 139 strains of Streptomyces bacteria were isolated from these samples. Moreover, researchers determined the physicochemical characteristics of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic Acid (DAP) forms were detected followed by the determination of then phenotypic characteristics. When these isolates were divided into 39 color groups, 20 strains were found to contain L-DAP and therefore were confirmed as members of the genus Streptomyces. The species were identified using the TAXON Software and similarity dendrograms were constructed by the Unweighted Pair-Group Method with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria and 16S rDNA gene was amplified by PCPv followed by restriction digestion with Bspl43I, Haelll, Mnll and SphI for Restriction Fragment Length Polymorphism (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, four groups of two or more members and six 1 -member groups were established according to the similarity ratio criterion. © Medwell Journals, 2014.
Trace Determination of Dna in an Amaryllidaceae Plant, Narcissus Tazetta by Square-Wave Stripping Voltammetry Based on Guanine Signal
(2007) Yardim, Y.; Şentürk, Z.; Özsöz, M.; Özdemir, K.; Atalan, E.
This study is, to our knowledge, the first application of electrochemical DNA biosensor for the quantification of DNA in plant extracts. The DNA biosensor was assembled by immobilizing the double-stranded fish sperm DNA on the surface of a singleuse pencil graphite electrode. Square-wave voltammetric method with the baseline correction was carried out to evaluate the oxidation signal of the guanine base. The experimental variables such as solution pH, buffer concentration, immobilization time, stirring speed, and square-wave parameters were optimized. The extremely low detection limit (0.36 ng/mL) was coupled to a good surface-to-surface reproducibility (a relative standard deviation of 8.4% for 7 repetitive measurements of 40 ng/mL). The renewable and low-cost DNA biosensor developed in this study using pencil graphite electrode was applied to the determination of DNA isolated from an Amaryllidaceae plant, Narcissus tazetta.
Use of Random Amplified Polymorphic Dna Pcr To Distinguish Micromonospora Species Isolated From Soil
(Medwell Journals, 2014) Ertas, M.; Ozdemir, K.; Atalan, E.
Recently with the advancement of molecular methods, studies that determine intraspecific genetic polymorphisms have yielded substantial results. The aim of this study was to use Random Amplified Polymorphic DNA PCR (RAPD-PCR) analysis to determine the differences among Micromonospora species isolated from the soil. M3 medium with antibiotics was used to isolate the species from the soil and 65 Micromonospora species were identified. Total DNA analysis was performed for the different color groups which were obtained as a result analysis of the species. Sm5R, Sm6F, DAF4, Ml 3, 27F and 1492R primers were used for RAPD-PCR analysis and the band profiles of the species were obtained. The dendrogram generated according to the simple matching coefficient method clearly showed the differences detected among the species. A total of seven groups were identified along with two main groups. Five of these groups had a single member, one had 3 and one had 2 members. © Medwell Journals, 2014.