Browsing by Author "Ataseven, Veysel Soydal"

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First Genetic Characterization of Equine Adenovirus Type 1 (eadv-1) in Turkey
(Elsevier Sci Ltd, 2012) Ataseven, Veysel Soydal; Oguzoglu, Tuba Cigdem; Basaran-Karapinar, Zeynep; Bilge-Dagalp, Seval
Equine adenovirus type 1 (EAdV-1) is a cause of repiratory tract infection in equids. In present study for the first time in Turkey, the prevalence of EAdV-1 in nasal swab samples obtained from horses showing respiratory symptoms was investigated by polymerase chain reaction (PCR), and molecular characterization of the hexon gene detected in the Turkish (TR) strain was performed. Overall, the prevalence of EAdV-1 was found low (1.4%) as indicated by a positive PCR reaction from the nasal swab extracts tested. Phylogenetic analysis based on the partial sequences of the hexon gene of a TR-EAdV-1 strain with those of previously isolated AdVs from different mammals and an EAdV-1 M1 strain showed that the EAdV-1 strains were placed into a unique cluster. Although the TR-EAdV-1 strain was closely related to CAV-1, CAV-2 and bat adenovirus reference strains, larger-scale studies are necessary to better understand the molecular epidemiology and population structure of EAdV-1 in Turkey. (C) 2011 Elsevier Ltd. All rights reserved.
Seroepidemiological Studies of Equine Herpesviruses 1 (ehv-1) and 4 (ehv-4) Infections in Working Horses From the Eastern Turkey
(Ankara Univ Press, 2010) Ataseven, Veysel Soydal; Bilge-Dagalp, Seval; Basaran, Zeynep; Keskin, Siddik
The objective of this research was to determine the presence of equine herpesvirus types 1 (EHV-1) and 4 (EHV-4) antibodies in local horses from five provinces in the East Anatolia region of Turkey. Blood samples were collected from 405 non-vaccinated horses living in Van, Bitlis, Mu, Erzurum, and Igdir, and were analyzed via a commercial enzyme-linked immunosorbent assay (ELISA). EHV-1- and EHV-4-specific antibodies were detected in 94 (23.2%) and 316 (78.0%) of the 405 tested sera, respectively. The seropositivity percentages for EHV-1-specific antibodies by province were as follows: 29.5% in Van, 6.1% in Bitlis, 5.1% in Mus 24.0% in Igdir, and 20.6% in Erzurum. EHV-4-specific antibodies were detected at levels of 87.2% in Van, 61.2% in Bitlis, 56.4% in Mus 84.0% in Igdir, and 52.9% in Erzurum. The present investigation demonstrated that EHV-1 and EHV-4 infections were more prevalent in the indigenous horse population from five provinces in the East Anatolia region of Turkey. Also, this data on the high seropositivity of both infections in two provinces (Van and Igdir) along the eastern border of Turkey may be also serve as a risk indicator for future introductions of some transboundary diseases via border trade.
A Snapshot Avian Surveillance Reveals West Nile Virus and Evidence of Wild Birds Participating in Toscana Virus Circulation
(Mary Ann Liebert, inc, 2017) Hacioglu, Sabri; Dincer, Ender; Isler, Cafer Tayer; Karapinar, Zeynep; Ataseven, Veysel Soydal; Ozkul, Aykut; Ergunay, Koray
Introduction: Birds are involved in the epidemiology of several vector-borne viruses, as amplification hosts for viruses, dissemination vehicles for the vectors, and sources of emerging strains in cross-species transmission. Turkey provides diverse habitats for a variety of wild birds and is located along major bird migration routes. This study was undertaken to provide a cross-sectional screening of avian specimens for a spectrum of vector-borne viruses. Materials and Methods: The specimens were collected in Hatay province, in the Mediterranean coast of the Anatolian peninsula, located in the convergence zone of the known migration routes. Generic PCR assays were used for the detection of members of Nairovirus, Flavivirus, and Phlebovirus genera of Flaviviridae and Bunyaviridae families. The circulating viruses were characterized via sequencing and selected specimens were inoculated onto Vero cell lines for virus isolation. Results and Discussion: Specimens from 72 wild birds belonging in 8 orders and 14 species were collected. A total of 158 specimens that comprise 32 sera (20.3%) from 7 species and 126 tissues (79.7%) from 14 species were screened. Eight specimens (8/158, 5%), obtained from 4 individuals (4/72, 5.5%), were positive. West Nile virus (WNV) lineage 1 sequences were characterized in the spleen, heart, and kidney tissues from a lesser spotted eagle (Clanga pomarina), which distinctly clustered from sequences previously identified in Turkey. Toscana virus (TOSV) genotype A and B sequences were identified in brain and kidney tissues from a greater flamingo (Phoenicopterus roseus), a great white pelican (Pelecanus onocrotalus), and a black stork (Ciconia nigra), without successful virus isolation. Partial amino acid sequences of the viral nucleocapsid protein revealed previously unreported substitutions. This study documents the involvement of avians in WNV dispersion in Anatolia as well in TOSV life cycle.