Browsing by Author "Avcioglu, Nermin Hande"

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Biofilm Formation of C. Albicans on Occlusal Device Materials and Antibiofilm Effects of Chitosan and Eugenol
(Mosby-elsevier, 2024) Ozarslan, Merve; Avcioglu, Nermin Hande; Can, Dilber Bilgili; Caliskan, Ahmet
Statement of problem. Microbial adhesion on occlusal devices may lead to oral diseases such as candidiasis. Whether chitosan and eugenol provide antibiofilm effects is unclear. Purpose. The purpose of this in vitro study was to evaluate the biofilm formation of C. albicans strains on occlusal device materials and the antibiofilm effects of chitosan and eugenol against C. albicans on these surfaces. Material and methods. A total of 88 specimens (5x10x2 mm) were produced from occlusal device materials with 4 production techniques: vacuum-formed thermoplastic (Group V), head-press (Group H), computer-aided design and computer-aided manufacture (CAD-CAM) (Group C), and 3-dimensionally (3D) printed (Group D) (n=22). After various finishing procedures, the surface properties of the specimens were evaluated by using surface free energy (SFE), surface roughness (SR) measurements, and elemental and topographic analysis. Biofilm formation of C. albicans strain and the antibiofilm effects of chitosan and eugenol against biofilm formation on these surfaces were also examined with a crystal violet assay. The distribution's normality was statistically analyzed with the Kolmogorov-Smirnov test. One-way and two-way analysis of variance with post hoc Tukey tests were used for statistical evaluations (alpha=.05). Results. Surface roughness values in Groups D and H were significantly higher than in other groups (P.05). While the highest surface free energy values (except gamma p) were in Group V, Group C had the highest gamma p. The lowest biofilm value appeared in Group H. Chitosan exhibited an antibiofilm effect in all groups except Group H, while eugenol was effective in all groups. Conclusions. The production method affected the susceptibility of occlusal device materials to the adhesion of C. albicans. Eugenol was an effective antibiofilm agent for device materials. (J Prosthet Dent 2024;131:144.e1 -e9)
Effect of Different Polishing Techniques on Surface Properties and Bacterial Adhesion on Resin-Ceramic Cad/Cam Materials
(Springer Heidelberg, 2022) Ozarslan, Merve; Bilgili Can, Dilber; Avcioglu, Nermin Hande; Caliskan, Secil
Objectives The aim of this study is to evaluate the adhesion of Streptococcus mutans on the surface of CAD/CAM materials with various surface treatments. Methods Vita Enamic, Lava Ultimate, and Cerasmart materials were used in this study. A total of 90 samples were prepared (n=10). After various finishing and polishing procedures (non-polished, manual polished, and glazed), surface roughness (SR) measurements, surface free energy (SFE), and elemental and topographic analysis with FIB-SEM/EDX were used to evaluate the samples' surface properties. To improve bacterial adhesion, CAD/CAM materials were covered with sterile artificial saliva containing mucin for pellicle formation and incubated for 1 h at 37 degrees C. Bacteria were then inoculated into the pellicle-coated specimens, and incubation was performed at 37 degrees C for 24 h. Bacterial adhesion was determined as x 10(5) CFU/mL and monitored using FIB-SEM analysis. The Kolmogorov-Smirnov test was used to statistically analyze the normality of the distribution; the groups were then compared using one-way ANOVA and Tukey's test. Results The SR of the control group was statistically higher in all materials (p < 0.05). There were no statistically significant differences in SR between all materials in the non-polished and manual polished groups (p > 0.05). The Vita Enamic control group exhibited the highest SFE value. The highest S. mutans adhesion was observed in non-polished (p< 0.05). Vita Enamic samples had higher CFU than other groups. Conclusions Non-polished surfaces showed higher SR and bacterial adhesion. Polishing processes affected the surface properties and bacterial adhesion.