Browsing by Author "Aydin, Funda"

Now showing 1 - 13 of 13

Caffeine Increased Antitumor Effects of Paclitaxel (Ptx) in Mcf-7 and Mda-Mb Breast Cancer Cells
(Marmara Univ, 2023) Aydin, Funda; Onder, Gozde Ozge; Goktepe, Ozge; Bitgen, Nazmiye
Although progress has been made in the diagnosis and treatment of breast cancer, which is one of the most important causes of women's health, it is still one of the leading causes of death in women. One of the most prominent causes for this is illness resistance to the medications employed in treatment. For this reason, the trend towards combination therapy research has increased in addition to conventional therapy. In this study, we aimed to investigate the effect of co-administration of caffeine (CAF) and paclitaxel (PTX), which is frequently used in breast cancer, on MDA-MB-231 and MCF-7 cells. For this purpose, 4 groups were determined as control, CAF, PTX and CAF+ PTX. MTT assay was used to assess cell viability and the appropriate dose for CAF was determined. The apoptotic effect of the drug combination on cell lines was evaluated with the TUNEL method, and it was determined at what stage it paused cell division by cell cycle analysis. According to the study's findings, the results indicated that CAF induced apoptosis in breast cancer cells and the best effect was in the group administered with PTX. Furthermore, it was discovered that CAF and PTX in the MCF-7 cell lines, both together and separately, blocked cell division in the S phase in MCF-7 cell lines. These results are promising for future studies that will prove the usefulness of CAF as an adjuvant in the treatment of breast cancer.
Cu2o-Cuo Ball Like/Multiwalled Carbon Nanotube Hybrid for Fast and Effective Ultrasound-Assisted Solid Phase Extraction of Uranium at Ultra-Trace Level Prior To Icp-Ms Detection
(Elsevier, 2020) Aydin, Funda; Yilmaz, Erkan; Olmez, Enver; Soylak, Mustafa
Multiwalled carbon nanotube/Cu2O-CuO ball like hybrid material (MWCNTs/Cu2O-CuO) synthesized using of a green hydrothermal process was evaluated as a new sorbent for solid phase extraction of uranium with inductively coupled plasma mass spectrometry (ICP-MS). According to our best knowledge, this new material was synthesized and used as solid phase extraction adsorbent for the first time in the literature. The characterization of new hybrid material was carried out using Raman spectroscopy, X-ray diffraction (XRD) and scanning electronic microscopy techniques (SEM, SEM-EDX and SEM-Mapping). The characterization results demonstrated that the new hybrid nanostructure was successfully obtained. The ultrasound-assisted solid phase extraction (UA-SPE) procedure was performed using 100 mg of new hybrid sorbent, 1000 mu L of a 0.1% (w/v) solution of alpha-benzoin oxime and 2000 mu L of phosphate buffer at pH 7.0 for separation and preconcentration of uranium. Uranium was eluted with 2 M HCl and the levels of uranium was measured by using ICP-MS. The linearity was observed between 2.5 and 100 ng mL(-1) concentration range with a good correlation coefficient (r = 0.999, n = 7). The obtained limit of detection and quantitation values were 0.52 and 1.70 ng mL(-1) respectively. Under optimum conditions, the preconcentration factor (PF) was calculated as 13.3. The accuracy of the developed method was assessed by analyzing of TMDA-62.2, TMDA-70.2 (Environment Canada) and GBW-07423 certified reference water and soil materials. The proposed UA-SPE-ICP-MS procedure developed with MWCNTs/Cu2O-CuO hybrid material was successfully applied to the analysis of uranium at ultra-traces levels in environmental water and geological rock samples.
Developing of an Eco-Friendly Liquid-Liquid Microextraction Method by Using Menthol-Based Hydrophobic Deep Eutectic Solvent for Determination of Basic Fuchsin Dye: Assessment of the Greenness Profile
(Springer, 2024) Aydin, Funda; Alkan, Volkan; Donmez, Fatih
Herein, we aimed to develop a new environmentally friendly liquid-liquid microextraction (LLME) method based on hydrophobic deep eutectic solvent (hDES) synthesized using biodegradable dl-menthol and decanoic acid for the spectrophotometric determination of toxic basic fuchsin dye in environmental water samples. The parameters affecting the extraction efficiency such as pH, mole ratio, and volume of hDES (1:2) and type and volume of organic solvent, sample volume, times of vortex, ultrasonic bath and centrifuge, ionic strength, and matrix effect were investigated and optimized. Under optimal conditions, the calibration curve showed linearity in the range of 7.4-167 mu g L-1 with a coefficient of determination of 0.9994. The limit of detection, intra-day and inter-day precision, and recovery values were 2.25 mu g L-1, 2.46% and 4.45%, and 105 +/- 3%, respectively. The preconcentration and enrichment factors were found to be 30 and 61.5, respectively. The proposed hDES-LLME methodology was successfully applied to the environmental water samples to detect toxic BF dye (95-105%). Finally, the ecological impact of the suggested method was evaluated using the analytical eco-scale (PPS:88), complementary green analytical procedure indexe (ComplexGAPI), and the Analytical GREEnness tool (0.63). The assessment results showed that the presented analytical method can be regarded as a green LLME approach for the determination of the BF in water.
Lipid/Zif-8 Biocomposites Based on Liposomes or Vesicles: in Situ Formation, and Preliminary Evaluation as Delivery Vehicles for Hydrophobic Drugs
(Wiley-v C H verlag Gmbh, 2025) Cano-Sarabia, Mary; Aydin, Funda; Meng, Lingxin; Gil-Bonillo, Marta; Fonseca, Javier; Dietrich, Manuela; Maspoch, Daniel
Integrating lipid self-assemblies with metal-organic frameworks (MOFs) creates biocomposites ideal for encapsulation, protection, and delivery of functional species. This can be achieved using preformed MOFs or through in situ MOF formation. Herein, the one-pot formation of ZIF-8 MOF particles in the presence of two lipid self-assemblies (vesicles or liposomes) is reported, generating two types of hybrid lipid/ZIF-8 biocomposites. Each lipid assembly can be used to encapsulate hydrophobic actives into the hybrid lipid/ZIF-8 biocomposites, demonstrated with Nile Red and Astaxanthin (ATX) as representative cargo. In vitro digestion of ATX-loaded hybrid lipid/ZIF-8 particles in simulated intestinal fluid (SIF) shows distinct release kinetics: liposome-based particles offer a more sustained release compared to vesicle-based biocomposites. Intriguingly, in various media (water, simulated gastric fluid, bicarbonate, and SIF), the sodalite ZIF-8 topology in liposome-based lipid/ZIF-8 particles undergoes a crystalline phase transition to the denser, more-stable phase ZIF-C. This phase transition, along with a deeper internalization of ATX in liposome-based particles, accounts for the differences in release kinetics. In summary, the study provides valuable insights for the synthesis of hybrid lipid/ZIF-8 biocomposites, the encapsulation of hydrophobic molecules, the importance of investigating potential crystalline phase transitions of MOFs in different media, and their potential as drug delivery vehicles.
Raman Spectroscopy: a Novel Experimental Approach To Evaluating Cisplatin Induced Tissue Damage
(Elsevier, 2020) Yay, Arzu; Onses, Mustafa Serdar; Sahmetlioglu, Ertugrul; Ceyhan, Ayse; Pekdemir, Sami; Onder, Gozde Ozge; Aydin, Funda
The aim of this work is to clarify the effect of curcumin and beta-carotene on cisplatin-induced tissue damage and to demonstrate the potential of Raman spectroscopy to detect tissue changes consistent with liver and kidney histopathology as a potential diagnostic adjunct. In the study, 56 Wistar albino female rats were used and randomly divided into 7 groups (n:8). Sham group received only sesame oil; Cisplatin group, received a single dose injection of cisplatin; Beta-carotene group, treated with beta-carotene orally; Cisplatin + Beta-carotene group, pretreated with beta-carotene 30 min prior to the cisplatin injection, then received cisplatin; Curcumin group, orally treated with curcumin; Cisplatin + Curcumin group, pretreated with curcumin 30min prior to the cisplatin injection, then received cisplatin. The second application was performed 1 week after the first application. One of the liver and kidney tissues was taken to 10% form for histopathological examinations and the others were taken to -80 degrees C for raman spectroscopy. Received sections were hematoxylin-eosin stained. The avidin-biotin peroxidase method was used for to investigate anti-TNF-alpha and IL1-beta activities. TUNEL method was applied to determine apoptotic cells. According to our histopathological findings, beta-carotene and especially curcumin have been found to possess hepatorenal protective activities. These datas were supported by the microscopic damage scores. Although some of these findings were observed in both the cisplatin + curcumin and cisplatin + beta-carotene groups, the incidence and severity of histopathological lesions were less than the cisplatin group. Both immunohistochemical studies and Raman spectroscopy results consistent with histopathological examination of hematoxylen-eosin stained sections. Raman spectroscopy represents a suitable tool to provide insights into structural factors involved in the mechanisms underlying antitumor effects of platinum drug.
Silica Gel-Immobilized 5-Aminoisophthalohydrazide: a Novel Sorbent for Solid Phase Extraction of Cu, Zn and Pb From Natural Water Samples
(Wiley, 2020) Aydin, Funda; Cakmak, Resit; Levent, Abdulkadir; Soylak, Mustafa
A novel silica sorbent, silica gel-immobilized 5-aminoisophthalohydrazide (SiO2-APH), was prepared by the condensation of 3-chloropropyl-functionalized silica gel with 5-aminoisophthalohydrazide (APH) derived from dimethyl 5-aminoisophthalate as a starting material and used for separation and preconcentration of Cu, Zn, and Pb metals in water samples using Flame Atomic Absorption Spectrometry (FAAS). The characterization of the new sorbent was carried out by Elemental Analysis, Thermogravimetric Analysis (TGA) and Fourier Transform Infrared Spectroscopy (FTIR). Important analytical parameters including as pH, amount of sorbent, type and amount of eluting solvent, sample volume, vortex and ultrasonic bath time, matrix ions that effect the developed SiO2-APH-solid phase extraction (SPE) method were investigated and optimum parameters were detected. Recoveries of examined metals were obtained as 98% for Cu and Pb and 101% for Zn. The relative standard deviation (RSD, n = 8) of Cu, Zn and Pb metals were 3.2, 2.8 and 1.6%, respectively. Limit of detections (LODs) (n = 10) were found as 2.7 mu g L-1 for Cu, 7.4 mu g L-1 for Zn and 3.5 mu g L-1 for Pb mu g L-1. The accuracy of the new method was assessed by analyzing of TMDA-51.4 and TMDA-70.2 certified reference materials. The results obtained for metals were in a good agreement with certified values. Addition/recovery test was applied to the real well, river, dam and stream water samples to check the accuracy of the method. The results showed that the developed SiO2-APH-SPE method can be effectively used as an alternative method for determination of Cu, Zn, and Pb metals in water samples.
A Simple and Novel Deep Eutectic Solvent Based Ultrasound-Assisted Emulsification Liquid Phase Microextraction Method for Malachite Green in Farmed and Ornamental Aquarium Fish Water Samples
(Elsevier Science Bv, 2017) Aydin, Funda; Yilmaz, Erkan; Soylak, Mustafa
In this work, the recently suggested concept of Deep Eutectic Solvents (DESs) as green and cheap extraction solvent was used for ultrasound-assisted emulsification liquid phase microextraction (UA-ELPME) of malachite green (MG) in farmed and ornamental aquarium fish water samples followed by UV-VIS spectrometry. In the DES-UA-ELPME, malachite green in sample solution (pH 3) was extracted into a well dispersed of microvolume of DES phase consisting of choline chloride and phenol and analyzed by UV-VIS spectrometry at 635 nm. A series of analytical factors such as pH and volume of sample solution, the type and volume of DES ultrasonication time and matrix effect that would affect the extraction efficiency was systematically examined and optimized. Under optimum conditions, the DES-UA-ELPME method offered a limit of detection (LOD) of 3.6 mu g L-1 and a relative standard deviation (RSD) of 2.7%. The procedure was validated with three aquarium and farmed fish water samples and quantitative recoveries (96-99%) were achieved. (C) 2017 Elsevier B.V. All rights reserved.
Supramolecular Solvent-Based Dispersive Liquid-Liquid Microextraction of Copper From Water and Hair Samples
(Royal Soc Chemistry, 2015) Aydin, Funda; Yilmaz, Erkan; Soylak, Mustafa
A supramolecular solvent based dispersive liquid-liquid microextraction (SM-DLLME) procedure has been established for the separation and preconcentration of Cu(II) before its determination by microsampling flame atomic absorption spectrometry. The proposed method involves the use of a supramolecular solvent in which reverse micelles of 1-decanol are dispersed in tetrahydrofuran. The Cu(II)pyrrolidinedithiocarbamate complex was formed to increase interactions with the supramolecular phase at pH 6. After the supramolecular solvent was added to the medium, the formation of micelles of nano and molecular size was observed in an ultrasonic bath. The solution was centrifuged, and the metal complex formed was extracted into the supramolecular solvent phase. Some analytical parameters that are important in the experiment were examined in detail. The detection limit (LOD), the quantification limit (LOQ) and the relative standard deviation (RSD) of the developed method were found to be 0.11 mg L-1, 0.34 mg L-1 and 2.2%, respectively. The preconcentration factor was 60. Addition/recovery studies were also performed in water and human hair samples. The accuracy of the proposed method was assessed by analyzing certified reference materials. The procedure was applied for the determination of copper in water and hair samples.
Supramolecular Solvent-Based Microextraction Method for Cobalt Traces in Food Samples With Optimization Plackett-Burman and Central Composite Experimental Design
(Royal Soc Chemistry, 2015) Aydin, Funda; Yilmaz, Erkan; Soylak, Mustafa
A new microextraction method based on formation of supramolecular solvent (Ss) was developed by using of chemometric optimization method for cobalt determination with microsampling flame atomic absorption spectrometry (MS-FAAS). 1-Decanol/THF was used to obtain supramolecular solvent, which ensure the formation of micelles in the nano and molecular size and to transfer the diethyldithiocarbamate (DDTC)-cobalt(II) complex from the aqueous phase to the extraction phase media. The optimization strategy was carried out by using of Plackett-Burman Design (PBD) and Central-Composite Design (CCD). Statistically significant parameters such as pH, the volume of ligand (DDTC), the volume of supramolecular solvent (1-decanol/THF) and centrifugation time were investigated by using of Plackett-Burman design. Central-composite design was used to determine optimal condition of these parameters. The optimum experimental conditions obtained were pH 6, 125 mu L of 1-decanol, 450 mu L of THF, 300 mL of DDTC (0.1%, w/v) and 8 min of centrifugation time. The relative standard deviation (RSD), limit of detection (LOD), limit of quantitation (LOQ) and preconcentration factor (PF) were 1.51% (n = 8, 94-98%), 1.89 mu g L-1, 6.32 mu g L-1 and 30 respectively. The method were applied to the certified reference materials of TMDA 53.3 water, TMDA 64.2 water, SPS-WW2 waste water, Oriental Basma Tobacco Leaves (INCT-OBTL-5) and scallion (salad onion), (NCS ZC73033) to validation. The microextraction method was also successfully applied to determine cobalt concentrations by microsampling FAAS in water, cereal, powdered beverage and fruit samples.
Therapeutic Potential of Hesperidin: Apoptosis Induction in Breast Cancer Cell Lines
(Pergamon-elsevier Science Ltd, 2023) Onder, Gozde Ozge; Goktepe, Ozge; Baran, Munevver; Bitgen, Nazmiye; Aydin, Funda; Yaya, Arzu
Hesperidin is a flavonoid commonly found in citrus fruits. Studies have shown that hesperidin has anti-inflammatory, analgesic, and antimicrobial properties, as well as its effectiveness in carcinogenesis. In this paper, we aim to investigate the molecular mechanisms of hesperidin-induced apoptosis in MCF-7 and MDA-MB-231 cancer cells.The inhibitory effect of hesperidin on cellular proliferation was evaluated with the MTT assay. Cell cycle analysis of hesperidin-treated cells was then performed, as well as immunocytochemical analysis of the effect on the apoptosis pathway (TUNEL, Bax, and Bcl-2 expression).Moreover, hesperidin induced cellular apoptosis in MCF-7 breast cancer cells by inhibiting Bcl-2 and enhancing Bax expression at protein levels. On the other hand, hesperidin caused apoptosis in the MDA-MB-231 breast cancer cell line, but it did not activate the Bax/Bcl-2 pathway. Hesperidin also induced cell cycle arrest at the S phase in the MCF-7 and MDA-MB-231 cell lines.These findings showed that hesperidin is a potential therapeutic candidate for preventing the progression of breast cancer. In addition, hesperidin could significantly stimulate the death mechanisms in ER/PR (+) MCF-7 cells by changing the expression balance of Bax and Bcl-2 proteins, but lead ER/PR (-) MDA-MB-231 breast cancer cells to apoptosis in a different way.
Tio2@zno Nanocomposite: Bifunctional Material for Solid Phase Extraction of U(Vi) and Th(Iv) and Photocatalytic Degradation of Organic Contaminant
(Springer, 2023) Aydin, Funda; Yilmaz, Erkan; Demirkiran, Guelay; Erbas, Zeliha; Vurucuel, Merve; Soylak, Mustafa
A bifunctional TiO2@ZnO nanorods hybrid material was synthesized via one-step hydrothermal synthesis and studied as an adsorbent for SPE of U(VI) and Th(IV) and as a photocatalyst for the photocatalytic removal of methylene blue (MB). The formation of TiO2@ZnO hybrid composite was confirmed by XRD, Raman, SEM, SEM-EDX and SEM-Mapping analysis. After determining the optimum values of the analytical parameters affecting the recovery of the analytes, the developed TiO2@ZnO hybrid NPs-SPE method was applied to natural waters with a high recovery efficiency (94-97%). The accuracy of the method was also verified by using CRM-TMDA 62.2 and TM-25.3 standards. Photocatalytic properties of the fabricated nanocomposite were investigated by degrading of MB under UV light. The results indicated that the synthesized TiO2@ZnO material can be used as a promising adsorbent/photocatalyst candidate for the adsorption of U(VI) and Th(IV) analytes in waters as well as the photocatalysis degradation of MB dyestuff.
Ultrasonic-Assisted Supramolecular Solvent-Based Liquid Phase Microextraction of Mercury as 1-(2 Complexes From Water Samples
(Taylor & Francis Ltd, 2016) Aydin, Funda; Yilmaz, Erkan; Soylak, Mustafa
A separation-preconcentration method based on supramolecular solvent ultrasonic-assisted liquid-phase microextraction (Ss-USA-LPME) for spectrophotometric determination of mercury as 1-(2-pyridylazo)-2-naphthol (PAN) chelates has been established. Red coloured Hg(II)-PAN hydrophobic complex was extracted into the supramolecular phase (1-decanol/THF) at pH 9.5. The extract was separated from aqueous phase by centrifugation, diluted with ethanol and determined by UV-Vis spectrophotometer at lambda(max) = 560 nm. The influences of important analytical parameters such as pH, amount of PAN, 1-decanol and THF, sample volume and matrix effects for the quantitative recoveries were examined and optimised. Under the optimised experimental conditions, the amount of ligand, 1-decanol and THF were 1.0 x 10(-4) M, 200 mu L and 300 mu L, respectively. The optimum time of ultrasonic bath and centrifugation were found as 2 min and 5 min. A linear calibration graph was obtained linearly in the concentration ranges of 8.31000 mu g L-1. The preconcentration factor was obtained as 20. The limit of detection (LOD) was 2.6 mu g L-1 with the relative standard deviation (RSD) of 2.4% for mercury (C = 100 mu g L-1, n = 7). The validity of the developed Ss-USA-LPME technique was checked with a certified reference material of NIST 1641d. The presented method has been successfully applied to the determination of mercury in water samples.
Vortex Assisted Deep Eutectic Solvent (des)-Emulsification Liquid-Liquid Microextraction of Trace Curcumin in Food and Herbal Tea Samples
(Elsevier Sci Ltd, 2018) Aydin, Funda; Yilmaz, Erkan; Soylak, Mustafa
We developed a new microextraction method for separation and preconcentration of curcumin using deep eutectic solvent known as green solvent. Deep eutectic solvent (DES) formed by mixing of choline chloride and phenol was used as an extraction solvent in microextraction study to extract the curcumin at pH 4.0. The curcumin concentration in enriched DES phase was analyzed by UV-Visible spectrophotometer. The effect of parameters such as pH, mol ratio of DES composition, volume of DES, volume of tetrahydrofuran (THF) and sample volume were examined. Interference effects of matrix components were investigated. The preconcentration factor was 12.5. The detection limit of method (n=10) was 2.86 mu g L-1 and the relative standard deviation (RSD, n=8) was 1.8%. The method was successfully applied to determination of curcumin in food and herbal tea samples. The mean recoveries were between 96% and 102% and standard deviations were found in the range of 1-6%.