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Browsing by Author "Aydin, G."

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    Article
    Antimicrobial Activity of Three Macrolepiota Species From Turkey
    (Chemical Publishing Co., 2008) Dulger, B.; Hacioglu, N.; Aydin, G.; Uzun, Y.
    Three Macrolepiota species (M. heimii, M. rachodes and M. puellaris) were tested for their antimicrobial activity by using the disc diffusion method. The ethanol extract from the fruit bodies of mushrooms were assayed against 16 microorganisms. In comparison with the test antibiotics amikacin, chloramphenicol and nystatin, the ethanol extract obtained from M. heimii presented significant activity against Micrococcus species and Citrobacter freundii. The extracts have weak anti-yeast activity against the yeast cultures.
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    Article
    Vector Construction Strategies for Transformation of Wheat Plant
    (Pakistan Agricultural Scientists Forum, 2013) Baloglu, M. C.; Battal, A.; Aydin, G.; Eroglu, A.; Oz, M. T.; Kavas, M.; Yucel, M.
    Gene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1 and TtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant.