Browsing by Author "Aykut, Handan"

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Bisphenol S Leads To Cytotoxicity-Induced Antioxidant Responses and Oxidative Stress in Isolated Rainbow Trout (Oncorhyncus Mykiss) Hepatocytes
(Springer, 2021) Kaptaner, Burak; Yilmaz, Can; Aykut, Handan; Dogan, Emine; Fidan, Ceylan; Bostanci, Muserref; Yildiz, Fatos
Background Bisphenol S (BPS) is a chemical compound that is utilized in the plastic industry as an alternative to bisphenol A (BPA). The toxic effects of BPS in fish is less known and limited. Therefore, in the present study, the influence of BPS on rainbow trout (Oncorhyncus mykiss) hepatocytes in vitro was investigated. Methods and results For this purpose the fish hepatocytes were isolated, and then the cultured cells were treated with increasing concentrations of BPS (0, 15.63, 31.25, 62.50, 125, 250, and 500 mu M) for 24 h. The cytotoxic impact of BPS was determined in the culture media using lactate dehydrogenase assay and then, the antioxidant defence indicators were assayed. The results showed that concentration-dependent increases were observed in the percentage of cytotoxicity. The superoxide dismutase activity was reduced, while the catalase and glutathione peroxidase activity increased with all of the BPS concentrations. The glutathione S-transferase (GST) activity significantly increased after a BPS concentration of 31.25 mu M or higher, while GST Theta 1-1 activity was decreased by the same concentrations of BPS. The reduced glutathione content significantly decreased with a BPS concentration of 31.25 mu M or higher, and the malondialdehyde content increased after BPS concentrations of 125, 250, and 500 mu M. Conclusions The findings determined herein suggested that BPS causes cytotoxicity in fish hepatocytes and can lead to oxidative stress, resulting hepatotoxic in fish. Thus, the utilization of BPS instead of BPA as safe alternative in industry should be re-evaluated in the future for environmental health.
Histochemical Changes, Cellular Dynamics and Antioxidant Responses in the Corpuscles of Stannius of the Female Lake Van Fish (Alburnus Tarichi Guldenstadt, 1814) (Cyprinidae) During Migration From Highly Alkaline and Brackish Water To Freshwater
(Wiley, 2022) Kaptaner, Burak; Dogan, Abdulahad; Aykut, Handan; Yildiz, Fatos; Donmez, Fatih; Dogan, Emine
The Lake Van fish (Alburnus tarichi) is an anadromous species, which is endemic to Lake Van Basin of Turkey. In spring, the fish migrates from Lake Van, which has highly alkaline and brackish water, to freshwater to spawn. During the anadromous migration of the female Lake Van fish, histochemical changes in the cell types, cellular dynamics, apoptosis, cell proliferation and antioxidant responses in the corpuscles of Stannius (CS) which are tiny endocrine glands related with calcium homeostasis were examined. When compared to the lake, calcium concentrations of ambient water and plasma increased considerably in freshwater. Freshwater dramatically increased the number of predominant type-I cells in the gland while decreased the type-II cells. A reduction in the cytoplasmic periodic acid-Schiff content of type-I cells was observed in freshwater. No cytoplasmic staining was found in cytoplasm of type-II cells in both lake and freshwater. Apoptotic cells were detected in fish sampled from both the lake and freshwater and a nonsignificant increase was observed in freshwater. The number of proliferating cells increased significantly in the freshwater, as well as antioxidant defences such as catalase, glutathione peroxidase and glutathione s-transferase activities, which indicates an elevated oxidative status. The findings suggest that type-I cells are possible source of a hypocalcin hormone and type-II cells are considered to be a likely precursor of type-I cells. Apoptosis and cell proliferation are basic mechanisms of gland remodelling. In addition antioxidant mechanisms play a role during the acclimation of the gland in different environments.
Investigation of Antioxidant System Indicators in Rainbow Trout (oncorhynchus Mykiss) Hepatocytes Exposed To Bisphenol F
(2020) Aykut, Handan; Kaptaner, Burak
Bisfenol F (BPF) günlük hayatta kullanılan birçok ürünün içeriğinde yer alan bir bisfenol A analoğudur. Bu çalışmada, primer kültürü yapılan gökkuşağı alabalığı (Oncorhyncus mykiss) hepatositleri, BPF'nin 7 farklı konsantrasyonu (0, 15.63, 31.25, 62.50, 125, 250 ve 500 μM)'na, 24 saat süre ile maruz bırakıldı. BPF'nin oluşturduğu hücresel hasar, laktat dehidrogenaz sitotoksisite testi ile tespit edildi. Daha sonra antioksidan savunma sistemi indikatörleri ölçüldü. Elde edilen sonuçlara göre, BPF'nin konsantrasyon ile ilişkili olarak hücrelerde hasar meydana getirdiği belirlendi. Süperoksit dizmutaz aktivitesinin, BPF uygulanan bütün gruplarda, istatistiksel olarak anlamlı bir şeklide arttığı gözlendi. Katalaz aktivitesinin, BPF'nin bütün konsantrasyonlarında, düştüğü tespit edildi. Redükte glutatyon seviyesinin, 15.63, 31.25, 62.50, 125 ve 250 μM BPF konsantrasyonlarında arttığı ancak 500 μM BPF konsantrasyonunda azaldığı gözlendi. Glutatyon peroksidaz aktivitesinin,15.63 µM BPF konsantrasyonunda değişmediği, diğer gruplarda ise istatistiksel olarak anlamlı bir şeklide arttığı tespit edildi. Glutatyon-S-transferaz aktivitesinin ise sadece 125 µM'lık BPF konsatrasyonunda yükseldiği, diğer gruplarda değişmediği gözlendi. Bu sonuçlar, BPF'nin balığın hepatositlerinde antioksidan dengeyi bozarak, hücresel hasara yol açtığını göstermektedir. Anahtar kelimeler: Antioksidan savunma sistemi indikatörleri, Bisfenol F, Gökkuşağı alabalığı, Hücre hasarı, Oncorhyncus mykiss
Toxicological Investigation of Bisphenol a and Its Derivates on Human Breast Epithelial (mcf-10a) Cells
(Pergamon-elsevier Science Ltd, 2025) Bakan, Buket; Kaptaner, Burak; Tokmak, Merve; Aykut, Handan; Mendil, Ali Sefa; Ozkaraca, Mustafa
Bisphenols can enter the body, where they have potential adverse effects on human health, via different routes such as inhalation, dermally or orally. They are known as endocrine disrupting chemicals that activate signaling pathways by mimicking the estrogen actions. In this study, we aimed to investigate effects of bisphenol A (BPA), and its analogues bisphenol F (BPF) and bisphenol S (BPS) on MCF-10A cells and their impact mechanisms on autophagy, apoptosis and reduced glutathion levels. In comparison of the cytotoxic effects, while BPF and BPS showed dose-dependent high toxicity on MCF-10A cells, BPA exerted cytotoxic effects only at the highest doses. Caspase 3 and LC3B are strongly and positively correlated with BPF exposures while significant changes were not detected in the BPA and BPS applied groups. It was clearly observed that BPF and BPS displayed more toxic effects than BPA on human breast cells that are important targets for the bisphenols. These findings provide data for understanding the mechanisms for BPA, BPF and BPS-induced toxicity on human breast cells.