Browsing by Author "Aypak, C."

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Antimicrobial Susceptibilities of Brucella Isolates From Various Clinical Speciemens
(Ivyspring International Publisher, 2011) Bayram, Y.; Korkoca, H.; Aypak, C.; Parlak, M.; Cikman, A.; Kilic, S.; Berktas, M.
Purpose: Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-Brucella agents and a possible new alternative tigecycline. Materials and Methods: A total of 56 Brucella isolates were enrolled the study and the identification of the isolates were based on conventional methods. In vitro activities of an-timicrobials were evaluated by the E test method. Results: All isolates were identified as B. melitensis. MIC90 values of doxycycline, strepto-mycin, rifampin, trimethoprim-sulfamethoxazole and tigecycline were 0.064 mg/L, 1 mg/L, 2 mg/L, 0.125 mg/L and 0.094 mg/L, respectively. Tigecycline had low MIC50 and MIC90 values against all B. melitensis strains; the highest MIC observed was 0.25 μg/mL. Conclusion: Our data suggest that tigecycline can be a therapeutic alternative option for the treatment of brucellosis. © Ivyspring International Publisher.
Antiviral Drug Resistance Rates Among Patients With Chronic Hepatitis B Infection
(Publishing Office of the University of Rzeszow, 2023) Özlük, S.; Bayram, Y.; Özkaçmaz, A.; Parlak, M.; Özdemir, A.; Aypak, C.
Introduction and aim. Chronic hepatitis B infection (CHB) affects millions of people around the world. Many clinicians find it challenging to choose therapeutic agents due to the mutations that occur in the hepatitis B virus (HBV) that cause drug resistance. Thus, the aim of this study was to determine the HBV resistance rates against the currently recommended first-line therapies in the region of our country where HBV prevalence is high. Material and methods. A total of 96 patients (56 men and 40 women) with HBV infection were enrolled in the study. The serum samples collected from those were analyzed with real-time polymerase chain reaction analysis followed by pyrosequencing (PyroStar HBV Drug Resistance Test, Altona Diagnostics, Germany) for drug resistance mutations associated with lamivudine, adefovir, telbivudine, entecavir, and tenofovir. Results. HBV drug-resistance mutations were investigated in 80 treatment-naïve and 16 treatment-experienced patients (6 entecavir, 4 PEGylated-interferon, 4 tenofovir, 2 lamivudine). None of the HBV-DNA samples had mutations cause to drug resistance were detected in any codons regions that were analyzed. Conclusion. Antiviral resistance poses serious obstacles for clinicians in the treatment of CHB. Determining whether antiviral resistance exists in HBV is critical to choose the appropriate treatment agent. © 2023 University of the Philippines Manila. All rights reserved.
Diagnostic Accuracy of Iga Anti-Tissue Transglutaminase in Celiac Disease in Van-Turkey
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Bayram, Y.; Parlak, M.; Aypak, C.; Bayram, İ.; Yılmaz, D.; Çıkman, A.
Although the IgA anti-tissue transglutaminase test (IgA anti-tTG) has been recommended as the first step in the diagnosis of celiac disease (CD), there are controversial data about the real accuracy of the test in clinical practice. Therefore we evaluated the sensitivity and specificity of the IgA anti-tTG in a group of patients who were suspected of having CD. The study was performed at Van Training and Research Hospital, Van-Turkey. Details of patients in whom the IgA anti-tTG was requested from January 2009 to April 2012 were obtained from databases. Duplicate requests were excluded. Histopathologic examination of duodenal biopsies and serologic evaluations were compared. A total of 1614 IgA anti-tTG were requested from different patients. In all, 49.6% of requests were in females and 29.8% from children under the age of sixteen. A total of 192 (11.9%) requests were found to be positive. Duodenal biopsies were performed to 61 (31.8%) of seropositive patients. The overall sensitivity and specificity of IgA antitTG were 93.3% and 9.5%. Our data have revealed that clinicians should be aware of solely relying on the results of the IgA anti-tTG test could result in unnecessary diagnostic procedures and treatments. © 2015 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Epidemiological Characteristics and Molecular Typing of Salmonella Enterica Serovar Typhi During a Waterborne Outbreak in Eastern Anatolia
(Maney Publishing, 2011) Bayram, Y.; Guducuoglu, H.; Otlu, B.; Aypak, C.; Gursoy, N. C.; Uluc, H.; Berktas, M.
In this study, we aimed to study the molecular and epidemiological characteristics of Salmonella enterica serovar Typhi (S. typhi) outbreak in Eastern Anatolia. Six hundred and thirty-seven patients from the same county with clinical diagnosis of typhoid fever were investigated with conventional methods from stool, urine and blood specimens. Antibiotic susceptibility tests and identifications were performed for positive specimens. Clonal relationships between the isolates were investigated using pulsed field gel electrophoresis (PFGE) method. A questionnaire was completed for the water consumption habits of patients. Of 91 culture positive specimens, 76 were blood, 13 were stool and 2 were urine. The isolates were resistant to ampicillin, ampicillin/sulbactam, chloramphenicol, cefuroxime, amikacin, gentamicin and trimethoprim-sulfamethoxazole. Although there was a single band difference in some isolates, PFGE results indicated that this was an outbreak caused by single strain according to the Tenover criteria. This outbreak thought to be associated with the consumption of tap water contaminated with sewage represents a breakdown of the basic public health and civil engineering infrastructure. Appropriate public health measures should be taken in order to avoid such outbreaks in the future.
Serum Adenosine Deaminase Activity in Cutaneous Anthrax
(International Scientific Literature Inc., 2014) Sunnetcioglu, M.; Karadas, S.; Aslan, M.; Ceylan, M.R.; Demir, H.; Oncu, M.R.; Aypak, C.
Background: Adenosine deaminase (ADA) activity has been discovered in several inflammatory conditions; however, there are no data associated with cutaneous anthrax. The aim of this study was to investigate serum ADA activity in patients with cutaneous anthrax. Material/Methods: Sixteen patients with cutaneous anthrax and 17 healthy controls were enrolled. We measured ADA activity; peripheral blood leukocyte, lymphocyte, neutrophil, and monocyte counts; erythrocyte sedimentation rate; and C reactive protein levels. Results: Serum ADA activity was significantly higher in patients with cutaneous anthrax than in the controls (p<0.001). A positive correlation was observed between ADA activity and lymphocyte counts (r=0.589, p=0.021) in the patient group. Conclusions: This study suggests that serum ADA could be used as a biochemical marker in cutaneous anthrax. © Med Sci Monit, 2014.