Browsing by Author "Azizoglu, Burcu Ergoz"

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Effects of Salinity on Gill Histology in Sailfin Velifera (Poecilia Velifera)
(Springer, 2024) Oguz, Ahmet R.; Sepil, Ahmet; Alkan, Zehra; Azizoglu, Burcu Ergoz; Sen, Fazil; Oguz, Elif Kaval
Fish live in aquatic areas with varying salinity from fresh water to seawater. Gills are one of the most important osmoregulatory organs for maintaining the salt-water balance. In this study, changes in the gills of velifera (Poecilia sphenops) acclimated to 20, 30, 40, and 50 ppt of saline in the laboratory were examined histologically and immunohistochemically. Mucus cells (MCs), proliferating cell nuclear antigen (PCNA), mitochondria-rich cells (MRCs), and heat shock proteins (HSP70) + cells were marked in the gill tissue, which underwent routine histological procedures, and the severity of histological alterations increase with the increase of the salinity. When the gills were examined histologically, it was determined that there was an increase in cells due to hyperplasia, especially in the primary lamellae. A rapid increase in MC and HSP70 + cell numbers was observed in the 20 and 30 ppt groups. Although PCNA + cells were present in each salinity group, they were observed in the salinity group up to 50 ppt. An increase in the number and volume of MRCs was observed with increasing salinity. Based on the histological and immunohistochemical results, it is thought that the cytological and molecular arrangements of cells in gills of sailfin velifera under different levels of salinity are essential for the regulation of ion and salt balance.
Histopathological Determination of Changes in Tissues of Lake Van Fish (Alburnus Tarichi (Güldenstädt, 1814)) Exposed To Esfenvalerate
(Taylor & Francis Ltd, 2024) Oguz, Elif Kaval; Alkan, Zehra; Oguz, Ahmet Regaib; Azizoglu, Burcu Ergoz; Orgi, Elif
Esfenvalerate is a widely used pesticide of the pyrethroid insecticide group. It is a hydrophobic pesticide with toxic effects on many aquatic organisms. In this study, the damage caused by esfenvalerate 0.158 mu g/L administered sublethally to the gills, liver, and kidneys of Lake Van fish was determined histopathologically. Lesions such as necrosis, hyperplasia, epithelial lifting, hypertrophy, and haemorrhage were observed in the gills exposed to esfenvalerate. Gill goblet cells increased after 24 h and decreased on the other days of esfenvalerate exposure. Histopathological changes such as haemorrhage, hypertrophy, necrosis, and cloudy degeneration were observed in the liver. In the kidneys, lesions such as haemorrhage, necrosis, cell degeneration, Bowman's capsule enlargement, and glomerulus atrophy were detected. These lesions increased in tissues depending on the length of application of esfenvalerate. As a result of this study, it was determined that esfenvalerate caused damage to the gills, liver, and kidneys in Lake Van fish depending on time. These anomalies observed in the gills, liver, and kidneys can be used as a bioindicator of esfenvalerate contamination in Lake Van.