Browsing by Author "Babaoglu, A. R."

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Crimean-Congo Hemorrhagic Fever Virus Infection in Domestic Ruminants in Van Province, a Non-Endemic Region in Turkey
(Agricultural Research Communication Centre, 2023) Babaoglu, A. R.; Oguz, F. Ertas; Kilinc, O. Orunc; Cetin, N.; Aslan, L.
Background: Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral disease of humans that is most widespread in Africa, Asia, the Middle East and Southeast Europe. The disease has continued to be a health problem in Turkey and other endemic countries in recent years. CCHF virus does not cause clinical disease in animal hosts but develops an antibody response and a transient viraemia. The aim of this study is to evaluate the status of CCHF infection serologically and virologically in livestock in the Van region, a non-endemic area in Turkey. Methods: A total of 491 whole blood and 491 serum specimens were collected from cattle, sheep and goats in different locations of Van province in Turkey from May 2020 to October 2021. To determine the CCHFV-specific antibody, all sera from animals were tested with a double-Ag ELISA-Ab kit. An RT-PCR assay was performed on total leucocyte samples for the virological study to detect the presence of European lineage-1 CCHFV nucleic acids and viraemic animals. Result: The prevalence of CCHFV-specific antibodies in animals was found to be 36.4%. The seroprevalence values detected were 11.6%, 64.45% and 81.63% in cattle, sheep and goats, respectively. In the RT-PCR assay, 1.22% (6/491) of the tested animals were found to be viraemic at the time of sampling.
First Detection of Ehrlichia Chaffeensis, Ehrlichia Canis, and Anaplasma Ovis in Rhipicephalus Bursa Ticks Collected From Sheep, Turkey
(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Ayan, A.; Celik, B. Aslan; Celik, O. Y.; Kilinc, O. Orunc; Akyildiz, G.; Yilmaz, A. B.; Babaoglu, A. R.
Anaplasmosis and ehrlichiosis are important tick -borne rickettsial diseases of medical and veterinary importance that cause economic losses in livestock. In this study, the prevalence of Anaplasma ovis, Ehrlichia canis and Ehrlichia chaffeensis was investigated in ticks collected from sheep in various farms in Van province, which is located in the Eastern Anatolian Region of Turkey. The ticks used in this study were collected by random sampling in 26 family farm business in 13 districts of Van province. A total of 688 ticks were collected from 88 sheep and 88 tick pools were created. All ticks identified morphologically as Rhipicephalus bursa. Phylogenetic analysis of Chaperonin and 16S rRNA gene sequences confirmed A. ovis, E. canis and E. chaffeensis in this study. Of the 88 tick pools tested, 28.41% (25/88) were positive for at least one pathogen. Anaplasma DNA was detected in five of the 88 pools (5.68%), E. canis DNA was detected in 19 of the 88 pools (21.59%), and E. chaffeensis DNA was detected in one of the 88 pools (1.14%) of R. bursa ticks. To our knowledge, this is the first report describing the presence of A. ovis, E. canis, and E. chaffeensis in R. bursa ticks collected from sheep in Turkey. Further studies are needed to investigate other co -infections in sheep in Turkey.