Browsing by Author "Babaoglu, Ali Riza"

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Genetic Variability of Pestivirus a (bvdv-1) Circulating in Cattle From Eastern Turkey
(Wiley, 2025) Abounaaja, Fatima; Babaoglu, Ali Riza
BackgroundBovine viral diarrhoea virus (BVDV) infection, caused by Pestiviruses A and B, with various clinical findings and causes significant economic losses. This disease is common in Turkey as well as in other countries, especially in European countries.ObjectiveThis study was designed to determine the genotypes of BVDVs and their variability among cattle in eastern Turkey.MethodsA total of 110 samples from 85 cattle suspected of BVDV infection were tested using RT-PCR with primers targeting the 5 ' UTR, autoprotease (Npro) and E2 gene regions of pestiviruses. Sequence and phylogenetic analyses were performed on the 5 ' UTR and Npro gene regions of these samples.ResultsAnalysis of 15 sequences obtained from 13 cattle revealed that Pestivirus A (BVDV-1) was responsible for the infection. In addition, the study identified subgenotypes BVDV-1a (n = 5), 1b (n = 5), 1d (n = 1), 1f (n = 1), 1l (n = 1) and 1r (n = 2). No evidence of infection with Pestivirus B (BVDV-2), Pestivirus D (Border disease virus) or Pestivirus H (HoBi-like virus/BVDV-3) was found.ConclusionThe significance of pestiviruses in causing genital and respiratory problems is once again emphasised, underscoring the necessity of including them in herd screening. Identifying BVDV genetic diversity both in Turkey and worldwide is crucial for developing effective protection, control and eradication strategies, particularly for vaccination programs. As a conclusion, the identification of BVDV-1a, 1b, 1d, 1f, 1l and 1r in the eastern provinces of Turkey points to an increase in BVDV-1 genetic diversity.
Investigation of Interleukins and Oxidative Stress Parameters in Cows Naturally Infected With Bovine Viral Diarrhea Virus
(Univ Agriculture, Fac veterinary Science, 2024) Oguz, Fatma Ertas; Babaoglu, Ali Riza; Turkan, Fikret; Oguz, Ercan; Demirel, Ahmet Fatih; Pacal, Nurettin
Bovine Viral Diarrhea Virus (BVDV) can evade the immune system by modulating cytokines production, therefore enabling the virus to establish persistent infections or exacerbate the severity of disease in infected cattle. The objective of this research was to investigate interleukins and oxidative stress parameters in cows naturally infected with BVDV. The study comprised of two groups: a naturally infected group of 15 cattle with clinical signs of BVDV infection or a history of abortion and confirmed positive for pestivirus by RT-PCR, and a control group of 15 cattle with no abortion anamnesis or clinical symptoms, confirmed negative by RT-PCR. Anti-inflammatory cytokines (IL-4, IL-20, IL-10) and pro-inflammatory ones (IL-1, IL-1(3, IL-6) values were measured in all samples using ELISA method. Additionally, the oxidative stress marker malonaldehyde (MDA) was measured in all samples using the HPLC method. MDA levels were significantly higher in the infected samples compared with the controls. While cytokine levels were elevated in the infected group, however, the differences were not statistically significant. This study found a positive relationship among anti-inflammatory and pro-inflammatory cytokines, as well as a negative relationship among IL-1(3 and IL-1. It was concluded that oxidative stress occurs in BVDV-infected cattle, and the interleukins measured appear to remain in equilibrium by inhibiting each other. Notably, IL-20 was measured for the first time in BVDVinfected cattle, making it an important finding. Altogether, it may be concluded on the basis of these results that cytokines are important in the evaluation of the disease process.
Prevalence of Ehrlichia Spp in Ticks Collected From Dogs in Province of Van in Turkey
(Health & Environment Assoc, 2019) Ayan, Adnan; Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Babaoglu, Ali Riza
Ticks Rhipicephalus sanguineus of Rhipicephalus strain of Ixodidae family are biological mediators to transfer of Rickettsia pathogens such as Ehrlichia and Anaplasma in animals In the present study, a total of 220 ticks were collected from 55 infested dogs. The ticks' taxonomy was carried out through microscopically. Additionally, DNA extraction from ticks was conducted and nested PCR was performed to differentiate the Ehrlischia spp. The obtained results indicates that collected ticks were belong to Rhipicephalus sanguineus of Rhipicephalus strain of Ixodidae family of ticks. The PCR results showed Ehrlichia spp. was present in ticks and E. canis was more common (50 of 220 ticks) than E. ewingii (3 of 220 ticks). In conclusion, E. canis was firstly detected in R. sanguineus tick in dogs in the Province of Van in Turkey. E. ewingii was firstly detected in R. sanguineus tick in dogs in Turkey. Its presence in dogs indicates the potential factor for public health hazard in Turkey.
Seroprevalence of Bovine Leukemia Virus in Cattle and Buffaloes in the Border Provinces of the Eastern Anatolia Region, Türkiye: Insights Into the Eradication of Infection
(Urmia Univ, 2024) Babaoglu, Ali Riza; Oguz, Fatma Ertas; Yilmaz, Volkan; Coskun, Nuvit; Abounaaja, Fatima
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, T & uuml;rkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA). For this purpose, a total of 1,033 serum samples were collected from 982 cattle and 51 buffaloes from the provinces of A & gbreve;r & imath; (n = 178), I & gbreve;d & imath;r (n = 252), Kars (n = 317), Van (n = 221), and Hakkari (n = 65) during 2021- 2022. In AGID and ELISA tests, seropositivity for BLV-specific antibodies was not detected in cattle and buffaloes from the mentioned provinces. This study revealed that BLV was not circulating in cattle and buffaloes in the easternmost border provinces of T & uuml;rkiye during the sampling period and contributed to determine the status of BLV in the mentioned region. Due to the presence of virus in other regions of T & uuml;rkiye and neighboring countries, Iran and Iraq, it is recommended to control animal movements, continue efforts to combat the transmission of the virus, and maintain control measures. (c) 2024 Urmia University. All rights reserved.