Browsing by Author "Babat, Ceylan Fidan"

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Comparison of Short-Term Physiological and Biochemical Effects of Drought Stress on Two Wheat Cultivars
(inst Tecnologia Parana, 2023) Yilmaz, Pinar; Babat, Ceylan Fidan; Yilmaz, Can
Drought stress, which is becoming more frequent and severe, restricts grain production. It is critical to choose wheat (Triticum aestivum L.) cultivars that are drought-resistant enough in progressively arid climates. Early physiological and biochemical responses were measured by applying drought stress to susceptible Bezostaja-1 and tolerant Tosunbey wheat cultivars with three different PEG-6000 concentrations to understand the reliability, selectivity and practicality of tested parameters. Depending on the elevated drought, lengths, fresh-dry-turgor weights, relative water content of both root and stem decreased, and chloropyll amounts increased similarly but in different dimensions in both cultivars. While a decrease was observed in the total thiol content of both, it was determined that this value was 10 times higher in Bezostaja-1 cultivar. While proline amounts peaked at the most severe drought, this value was found to be 2.5 times higher in Bezostaja-1 than in Tosunbey. The protein content is 35% higher in Bezostaja-1. In contrast to glutathione S-transferase theta1 (GSTT1) isozyme, total GST activity increased 53.1% in Bezostaja-1 and 33.6% in Tosunbey, compared to control. This average values are about three times higher in Bezostaja-1 compared to Tosunbey. Of the experimental groups whose band profiles were generated by SDS-PAGE and compared with UPGMA and neighbor-joining methods, moderate to high drought samples of Bezostaja-1 showed explicitly different results and possibly a metabolically distinct response from all others. Besides frequently used ones, total thiol content, total GST activity and changes in the protein bands could be used as useful parameters in the selection of drought-resistant cultivars.
Dondurarak Depolamaya Karşılık Geleneksel Depolama Yönteminde Nar ve Portakal Sularının Toplam Antioksidan Kapasitelerindeki Değişimler
(2023) Babat, Ceylan Fidan; Gök, Rümeysa; Kadıoğlu, Kübra; Tatli, Hatice; Yılmaz, Esra; Çetin, Doğan; Yılmaz, Can
Bu çalışmanın amacı, hasat sonrası ürün kaybının önlenmesi için Akdeniz bölgesindeki evlerde halen kullanılan çam iğneleri ve pamuk tohumları kombinasyonundan oluşan geleneksel depolama ve dondurucuda (-20 ̊C) depolama yöntemlerini nar ve portakal meyvelerinin antioksidan kapasitesini korunma yetenekleri açısından karşılaştırmaktır. Meyveler geleneksel depolama ve dondurucuda depolama grupları olmak üzere iki gruba ayrılmıştır. Geleneksel depolama grubunda; meyveler bir bütün olarak, tasarlanan kasalarda depolanmıştır. Dondurucuda depolama grubunda ise meyveler sıkılmış ve meyve suları -20 ̊C'de depolanmıştır. İlk antioksidan kapasite ölçümleri meyve örnekleri laboratuvara gelir gelmez 2,2-difenil-1-pikrilhidrazil yöntemi kullanılarak yapılmış, ikinci ve üçüncü ölçümler ise sırasıyla 3. ve 6. aylarda gerçekleştirilmiştir. Sonuçlar her iki depolama yönteminin 3 aylık depolama süresince toplam antioksidan kapasiteyi koruma yetenekleri açısından benzer olduklarını, geleneksel depolama yönteminin en az üç aylık depolama için güvenilir bir şekilde kullanılabileceğini göstermiştir. Altı ay sonunda ise geleneksel depolama ile depolanan nar ve portakalların antioksidan aktivitelerinde %20 ve %33 oranında kayıp gerçekleşirken dondurucuda depolanan meyve sularının antioksidan aktivitelerinde istatistiksel olarak anlamlı bir değişim gözlenmemiştir.
In Vitro and in Silico Evaluation of Inhibitory Effects of Bisphenol Derivatives on Acetylcholinesterase of Electric Eel (Electrophorus Electricus L.)
(Elsevier Science inc, 2022) Yilmaz, Can; Khorsheed, Waleed Mohammed; Babat, Ceylan Fidan
The inhibitory effects of bisphenol A (BPA) and bisphenol S (BPS), which are common pollutants, especially in marine and freshwater, on the electric eel acetylcholinesterase (AChE) activity were studied in vitro and in silico. Both produced full non-competitive inhibition, but the Ki value of BPA was half that of BPS. Molecular docking analyses revealed that both interact with residues W286, F297, Y337, F338 in the PAS and ABS regions in the middle and entrance of the active site gorge, and that BPS also has hydrogen bond with S203 of the catalytic triad. The surge at IC50 values of both compounds with an inflection point at pH: 8.2 suggested that Y124 and/or Y337 in the narrow gorge are primary structural factors in binding. Less effective inhibition of BPS, especially at 25-30 degrees C, the temperature at which enzyme activity peaks, was attributed to the conformation of the narrow gorge. Homology analyses for AChE initially revealed a significant degree of identity, particularly in the alpha/ beta hydrolase domain, which also comprises the active site, with sequences from seven distinct teleost species of various environments. Finally, it was discovered for the first time that BPS, like BPA, is a significant inhibitor of AChE, and this was confirmed by in vitro and in silico analyses done at various pH and temperature levels. It was concluded that this effect might also apply to AChE of most other bony fish.
A Thiophene Derivative, 2-Bromo Has Effective Anticancer Potential With Other Biological Properties
(Wiley-v C H verlag Gmbh, 2022) Yilmaz, Can; Pirdawid, Ahmed Othman; Babat, Ceylan Fidan; Konus, Metin; Cetin, Dogan; Kivrak, Arif; Kizildogan, Aslihan Kurt
Thiophene derivatives are studied intensively due to their anticancer, antimicrobial, antioxidant and some other clinically important properties. In this study, the biological properties of a new thiophene derivative, so called BMPT, with a moderate drug score of 0.29 were tested. BMPT showed selective cytotoxicity for LnCap, HepG2 and Caco-2 cancer cell lines with EC50 values of 138.573 mu M, 185.931 mu M, and 108.657 mu M, respectively, but not on the control cell line HEK293. The increased expressions were detected for caspase3, caspase8, caspase9 and Bax; but Bcl-2 expressions were decreased at cancer cell lines. BMPT decreased the total thiol content and total GST activities at HepG2 47 % and 40 %, respectively, and, molecular docking supported inhibitory models for GSTP1-1, GSTA1-1 and GSTM2-2 were mixed, uncompetitive and uncompetitive, respectively. BMPT also showed potent antibacterial activity against Staphylococcus aureus. Related with its selective inhibition on GST activities, BMPT is proposed to have significant apoptotic effect leaded by changes, especially, in caspase 3, Bcl-2 and Bax expressions.