Browsing by Author "Badak, M. Utku"

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Fabrication of Molecularly Imprinted Cotton Fibers for Quantification of Streptomycin in Honey Samples
(Wiley, 2023) Zengin, Adem; Badak, M. Utku; Bilici, Mustafa; Aktas, Nahit
Streptomycin is a type of aminoglycoside used in treatment of several infections and with serious side effects in humans and animals, such as ototoxicity and nephrotoxicity. In the present study, we focused on the preparation of streptomycin-imprinted cotton fiber for rapid, sensitive and selective quantification of streptomycin in honey samples. The imprinted cotton fibers were characterized by attenuated total reflectance-Fourier transform spectroscopy, scanning electron microscopy, and x-ray photoelectron spectroscopy. In addition, the imprinted cotton fibers showed high adsorption capacity (95.4 mg/g) with an imprinting factor of 3.69 and had satisfactory regeneration ability of up to 10 regenerations without significant change in adsorption capacity. The imprinted cotton fibers showed good linearity with a correlation coefficient of 0.9989 and limit of detection was determined to be 0.29 ng/mL for streptomycin in honey samples. The proposed method showed acceptable recovery ranges (98.0%-100.3%) with lower inter-day and intra-day precisions. Moreover, the performance of the proposed method was compared to the ELISA method. The results showed that the present method is as sensitive and selective as ELISA. It is concluded that the imprinted cotton fibers could be a potential alternative to antibiotic quantification based on traditional immunoassays or chromatographic techniques.
Preparation of Molecularly Imprinted Pdms Elastomer for Selective Detection of Folic Acid in Orange Juice
(Elsevier Science Bv, 2019) Zengin, Adem; Badak, M. Utku; Bilici, Mustafa; Suludere, Zekiye; Aktas, Nahit
Herein, it is reported an effective method to prepare novel molecularly imprinted polymers (MIP) on poly(dimethylsiloxane) (PDMS) elastomer via a combination of non-covalent imprinting approach and surface initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization for sensitive and selective detection of folic acid (FA). For this purpose, 2-(2-Methoxyethoxy) ethyl methacrylate (MEOMA), ethylene glycol dimethacrylate (EGDMA), azobisisobutyronitrile (AIBN), FA and dimethylsulfoxide (DMSO) were used as functional monomer, cross-linker, initiator, template molecule, and porogen, respectively. The adsorption behavior followed the Scatchard equation between FA and PDMS-MIP with a saturation adsorption capacity of 4.51 mg/g and pseudo-second-order kinetics with 60 min equilibrium adsorption time. Furthermore, PDMS-MIP elastomer was successfully applied for selective extraction and detection of FA from orange juice with sufficient recovery (95.5-100.5%) and relative standard deviation less than 7.0%. The limit of detection (LOD) for FA was found to be 0.0031 mu g/mL with a linear range between 0.01 and 100 mu g/mL and a correlation coefficient of 0.9997. Results showed the proposed method could easily, efficiently and selectively extracted FA from complex media. Note that this novel proposed method will open a new way to detect any selected molecules such as pesticide, protein, drug, etc. using molecularly imprinted PDMS elastomer materials.
Selective Separation and Determination of Quercetin From Red Wine by Molecularly Imprinted Nanoparticles Coupled With Hplc and Ultraviolet Detection
(Wiley-v C H verlag Gmbh, 2018) Zengin, Adem; Badak, M. Utku; Aktas, Nahit
In this study, a highly sensitive and selective sample pretreatment procedure using molecularly imprinted silica nanoparticles was developed for the extraction and determination of quercetin in red wine samples coupled with high-performance liquid chromatography with ultraviolet detection. The imprinted silica nanoparticles were prepared in the presence of N-acryoyl-l-aspartic acid (functional monomer), quercetin (template), azobisisobutyronitrile (initiator) and methylene bisacrylamide (cross-linker) and methanol/water (porogen) via surface-initiated reversible addition-fragmentation chain transfer polymerization. Surface characterization was performed and several imprinting parameters were investigated, and the results indicated that adsorption of quercetin on the imprinted silica nanoparticles followed a pseudo-first-order adsorption isotherm with a maximum adsorption capacity at 26.4mg/g within 60min. The imprinted silica nanoparticles also showed satisfactory selectivity towards quercetin as compared with its structural analogues. Moreover, the imprinted nanoparticles preserved their recognition ability even after five adsorption-desorption cycles. Meanwhile, the nanoparticles were successfully applied to selective extraction of quercetin from red wine with a high recovery (99.7-100.4%). The limit of detection was calculated to be 0.058g/mL with a correlation coefficient 0.9996 in the range of 0.2-50g/mL. As a result, the developed selective extraction method using molecular imprinting technology simplifies the sample pretreatment procedure before determination of quercetin in real samples.
Synthesis of Magnetic Halloysite Nanotube-Based Molecularly Imprinted Polymers for Sensitive Spectrophotometric Detection of Metoclopramide in Urine Samples
(Elsevier, 2020) Bilici, Mustafa; Badak, M. Utku; Zengin, Adem; Suludere, Zekiye; Aktas, Nahit
A novel molecularly imprinted polymer was synthesized on magnetic halloysite nanotube via surface initiated reversible addition-fragmentation chain transfer polymerization in the presence of 2-aminoethylmethacrylamide, 2-Cyano-2-propyl benzodithioate, ethylene glycol dimethacrylate (EGDMA) and azobis(isobutyronitrile) for sensitive and selective spectrophotometric determination of metoclopramide in urine samples. The synthesized imprinted polymer was characterized by several surface characterization techniques and the results indicated there was a thin polymer network on the magnetic halloysite nanotube. The rebinding properties of the molecularly imprinted magnetic halloysite nanotube were also investigated in detail and the maximum adsorption capacity and imprinting factor were found to be 37.8 mg/g and 4.51, respectively. The application of the proposed method was carried out by enrichment and spectrophotometric determination of metoclopramide via formation of a charge transfer complex between picric acid and eluted metoclopramide. Under the optimized conditions, the calibration curve was linear in the concentration range of 5.0-150.0 ng/mL and the limit of detection and the limit of quantification were calculated to be 1.5 ng/mL and 4.95 ng/mL, respectively. The inter-day and intra-day precisions were below 5% and recoveries were between 92.8% and 99.2%. The results showed that the proposed method increased the sensitivity and selectivity for spectrophotometric determination of metoclopramide.