Browsing by Author "Bakan, Nuri"

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Can Zaprinast and Avanafil Induce the Levels of Angiogenesis, Bone Morphogenic Protein 2, 4 and 7 in Kidney of Ovariectomised Rats
(Taylor & Francis Ltd, 2022) Huyut, Zubeyir; Bakan, Nuri; Yildirim, Serkan; Akbay, Halil Ibrahim; Huyut, Mehmet Tahir; Ahlatci, Adem; Ucar, Bunyamin
Objective: This study investigated effects of zaprinast and avanafil on angiogenesis, vascular endothelial growth factor (VEGF), bone morphogenic protein (BMP) 2, 4 and 7. Methods: Female rats were randomly divided into four groups (n = 6). Sham; abdomen was approximately 2 cm opened and closed. Ovariectomised (OVX); abdomen was opened 2 cm and the ovaries were cut. OVX + zaprinast and OVX + avanafil groups; after the same procedure with OVX, 10 mg/kg zaprinast and avanafil were orally administered for 2 month, respectively. Angiogenesis and the levels of VEGF, BMP2, 4 and 7 were determined. Results: VEGF, BMP2, 4 and 7 levels in OVX + zaprinast and especially OVX + avanafil groups were higher than the sham and OVX (p < .05). However, only VEGF and BMP2 levels in OVX + zaprinast group were significant according to sham (p < .05). Also, angiogenesis in OVX + zaprinast and OVX + avanafil groups was dominant according to sham and OVX (p < .05). Conclusions: Zaprinast and avanafil induced BMP2, 4 and 7 levels synergistically with increased VEGF and angiogenesis in renal tissue.
Do Avanafil and Zaprinast Exert Positive Effects on Bone Tissue Via the Nitric Oxide/Cyclic Guanosine Monophosphate/Protein Kinase-G Signaling Pathway in Rats With Ovariectomy-Induced Osteoporosis
(Univ Sao Paulo, Conjunto Quimicas, 2021) Huyut, Zubeyir; Bakan, Nuri; Yildirim, Serkan
Phosphodiesterase-5 inhibitors (PDE-5Is) exert positive effects on bone healing and mineralization by activation the nitric oxide/cyclic guanosine monophosphate/protein kinase-G (NO/cGMP/PKG) signaling pathway. In this study, the effects of zaprinast and avanafil, two PDE-5Is, on the NO signaling pathway, estrogen levels, selected bone formation and destruction marker levels, whole-body bone mineral density (WB-BMD), right femur trabecular bone thickness (RF-TBT) and epiphyseal bone width, angiogenesis in the bone-marrow, and selected oxidative stress parameter levels were investigated in rats with ovariectomy-induced osteoporosis. Twenty four adult rats (8 months old) were equally divided into four groups. The first group was the sham operated group. Groups 2, 3 and 4 included ovariectomized rats. At six months after ovariectomy, the 3rd and 4th groups were administered 10 mg/kg zaprinast and avanafil daily as a single dose for 60 days, respectively. Increases in the activity of the NO/cGMP/PKG signalling-pathway, C-terminal collagen peptide levels, angiogenesis in the bone marrow, RF-TBT, epiphy seal bone width and WB-BMD were observed compared to the ovariectomized positive control group (OVX), while the pyridinoline and deoxypyridinoline levels were decreased in the OVX+zaprinast and OVX+avanafil groups (p<0.05). The malondialdehyde, ubiquinone10/ubiquino110 and 8-hydroxy-2-deoxyguanosine/10(6) deoxyguanos ine levels were also increased in the ovariectomized groups compared to the sham group (p<0.05). Based on these results, the levels of bone atrophy and some markers of oxidative stress were increased due to acute estrogen deficiency induced by ovariectomy, but zaprinast and avanafil administration significantly prevented these changes.
Effects of the Phosphodiesterase-5 (pde-5) Inhibitors, Avanafil and Zaprinast, on Bone Remodeling and Oxidative Damage in a Rat Model of Glucocorticoid-Induced Osteoporosis
(int Scientific information, inc, 2018) Huyut, Zubeyir; Bakan, Nuri; Yildirim, Serkan; Alp, Hamit Hakan
Background: The aim of this study was to evaluate the effects of the phosphodiesterase-5 (PDE-5) inhibitors, zaprinast and avanafil, on NO signalling pathway, bone mineral density (BMD), epiphyseal bone width, bone marrow angiogenesis, and parameters of oxidative stress in a rat model of glucocorticoid-induced osteoporosis (GIOP). Material/Methods: Twenty-four 8-month-old male rats in four groups were given a single daily treatment during a 30-day period: an (untreated) control group (n= 6): a dexamethasone-treated group (120 mu/kg) (n= 6); a group treated with dexamethasone (120 mu/kg) and zaprinast (10 mg/kg) (n= 6): and a group treated with dexamethasone (120 mu/kg) and avanafil (10 mg/kg) (n= 6). Rat whole body bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DEXA), and bone histology was performed. Also, selected oxidative stress parameters by HPLC method and the other biochemical parameters by ELISA method were measured. Results: The GIOP model rats treated with zaprinast and avanafil showed a significant increase in NO, cyclic guanosine monophosphate (cGMP), and protein kinase G (PKG) (NO/cGMP/PKG) signaling-pathway components, and in C-terminal telopeptide of type I collagen (CTX-1), bone marrow angiogenesis, BMD, and epiphyseal bone width, compared with the (untreated) control rats (p<0.05). Levels of pyridinoline (PD) and deoxypyridinoline (DPD) were significantly reduced in the dexamethasone + zaprinast, and dexamethasone + avanafil treatment groups (p< 0.05). Malondialdehyde (MDA), ubiquinone-10 (CoQ10), ubiquinol CoQ10 (CoQ10H), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were significantly increased in the dexamethasone-treated group, compared with the (untreated) controls (p< 0.05). Conclusions: In the GIOP rat model, markers of oxidative stress and bone atrophy were significantly reduced by treatment with the PDE-5 inhibitors, zaprinast and avanafil.
Lipid Peroxidation and Dna Oxidation Caused by Dental Filling Materials
(Elsevier Taiwan, 2017) Yildiz, Mehmet; Alp, Hamit Hakan; Gul, Pinar; Bakan, Nuri; Ozcan, Mutlu
Background/purpose: The two direct dental restorative materials most commonly used today are silver-mercury amalgam and resin-based composites. The purpose of this study was to examine the effects of these two restorative materials and substances released by these into the oral environment on lipid peroxidation and DNA oxidation after entering the blood circulation. Materials and methods: Blood samples from 41 patients were collected before and 24 hours after the application of these restorative materials. The 8-hydroxydeoxyguanosine/deoxyguanosine ratio in these samples was measured to determine oxidative DNA damage, and malondialdehyde levels were measured to define lipid peroxidation. The paired samples t test and Pearson correlation analysis were used for the analysis of variables (alpha = 0.05). Results: While no statistically significant difference was observed after amalgam filling application in terms of DNA oxidation, a significant difference was observed after composite application (P < 0.05). Furthermore, a significant increase was determined in malondialdehyde levels of two materials (P < 0.05). In both amalgam and composite applications, a significant difference was observed before and after application in terms of released substances (mercury and unpolymerized monomer, respectively, P < 0.001). Conclusion: Mercury increased lipid peroxidation and Bis-GMA and TEGDMA dental resins increased both lipid peroxidation and DNA oxidation markers. (C) 2017 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.
Oxidative Stress Before and After Surgery in Benign Prostatic Hyperplasia Patients
(Wiley, 2019) Ercan, Mehlika; Alp, Hamit Hakan; Kocaturk, Huseyin; Bakan, Nuri; Gul, Mustafa
The aim was to assess oxidative stress in benign prostatic hyperplasia patients and also to evaluate the effect of operation in late (60 days) post-operative period. This study was conducted with 16 patients with benign prostatic hyperplasia and 16 healthy subjects. Serum malondialdehyde, blood 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine, erythrocyte superoxide dismutase, serum total coenzyme Q(10) and coenzyme Q(10) levels were measured. Independent samples t test was used to analyse the differences between control group and patients, while paired t test was used to analyse the differences between pre-operative and post-operative periods. Malondialdehyde and total coenzyme Q(10) levels were lower in patients, while 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine level was increased. However, superoxide dismutase activity and coenzyme Q(10) levels did not differ. After 60 days of operation, 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine and superoxide dismutase activity decreased, while total coenzyme Q(10) level increased. However, malondialdehyde and coenzyme Q(10) levels were not affected. The international prostate symptom scores of the patients were also decreased after the operation. The results suggest that blood 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine level may reflect the oxidative stress better than the malondialdehyde level, and surgical operation attenuates the oxidative stress in late post-operative period in benign prostatic hyperplasia patients.
Stimulating Effects of Vardenafil, Tadalafil, and Udenafil on Vascular Endothelial Growth Factor, Angiogenesis, Vitamin D3, Bone Morphogenic Proteins in Ovariectomized Rats
(Taylor & Francis Ltd, 2022) Huyut, Zubeyir; Alp, Hamit Hakan; Bakan, Nuri; Yildirim, Serkan; Sekeroglu, Mehmet Ramazan
Objective: This study investigated the effect of vardenafil, tadalafil, and udenafil from phosphodiesterase-5 inhibitors (PDE-5Is) on bone morphogenic-protein (BMP)2 and 4 levels, along with angiogenesis in ovariectomized rat's kidney. Method: Rats were randomly divided into five groups (n = 10). Sham: abdomen was opened, and closed. OVX: ovaries were removed. OVX + vardenafil, OVX + tadalafil, and OVX + udenafil groups: ovaries were removed and closed, and after 6 months from postoperative, 10 mg/kg of vardenafil, tadalafil, and udenafil were administrated as daily a single-dose for 60 days, respectively. Histopathologic and immunohistochemical examinations were performed for angiogenesis, and biochemical analysis for vascular endothelial growth-factor (VEGF), VitaminD(3), BMP2 and 4 levels in rat's kidney. Results: VEGF, BMP2 and 4, VitaminD(3), and angiogenesis were high in the all inhibitor groups compared with the sham and OVX (p < .05). However, BMP4 levels were only high in the OVX + tadalafil group (p < .05). Conclusion: The results indicated that vardenafil, udenafil, and especially tadalafil increased VEGF, BMP2, and VitaminD(3) levels.
Zaprinast and Avanafil Increase the Vascular Endothelial Growth Factor, Vitamin D3, Bone Morphogenic Proteins 4 and 7 Levels in the Kidney Tissue of Male Rats Applied the Glucocorticoid
(Taylor & Francis Ltd, 2022) Huyut, Zubeyir; Bakan, Nuri; Akbay, Halil Ibrahim; Yildirim, Serkan; Sekeroglu, Mehmet Ramazan
Objective: This study investigated effect of zaprinast and avanafil on vascular endothelial growth factor (VEGF), bone morphogenic protein (BMP) 4 and 7, and vitamin D-3 levels against the negative effect of dexamethazone. Method: Rats were randomly divided into four groups (n = 6). Control: Empty a syringe was immersed and removed subcutaneously. Dexamethasone (DEX): 120 mu g/kg DEX was injected subcutaneously once a day for 28 days. DEX + zaprinast and DEX + avanafil groups: 10 mg/kg zaprinast and avanafil were administrated to rats in addition to the same procedure in the DEX, respectively. VitaminD(3), VEGF, BMP4 and 7 levels by enzyme linked immunosorbent assay (ELISA) and angiogenesis by histopathological/immunohistochemical were evaluated. Results: BMP4 values in the DEX were lower than the other groups (p < .05). DEX + zaprinast and DEX + avanafil exhibited an increase in all the parameters compared to the control and DEX (p < .05). However, these were not significant for the DEX + zaprinast (p > .05). Also, there was a significant increase in angiogenesis in the DEX + zaprinast and DEX + avanafil. Conclusion: Zaprinast and significantly avanafil induced vitamin D-3, BMP4 and 7 levels by increasing angiogenesis in renal.