Browsing by Author "Bakir, Ahmet"

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Antioxidant and Antiradical Properties of Rhabdosciadium Anatolyi Flowers and Contents of Vitamin, Trace Element and Mineral
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2023) Bakir, Ahmet; Ekin, Suat; Firat, Mehmet
The aim of this study was to investigate the antioxidant and antiradical activity of the Rhabdosciadium anatolyi ( R. anatolyi) flowers, an endemic plant grown in the Eastern Anatolia Region of Turkey, by determining the level of vitamins E and C, mineral (Ca, Na, Mg, P, K), and trace elements (Mn, Zn, Cu, Fe, V, Cr, Mo, Co, Sr, Pb, Ti, Tl, Sn, Cd, As). Within the scope of the study, mineral and trace element analyzes were carried out by ICP-OES and AAS, Vitamin E by HPLC, Vitamin C, total phenolic content, total flavonoid, antioxidant capacity, hydrogen peroxide, DPPH, ABTS, superoxide, hydroxyl and hemolysis of erythrocytes with phenylhydrazine, radical scavenging activity of the R. anatolyi flowers methanol extract were determined spectrophotometrically. According to the results, a-tocopherol, ascorbic acid, phenolic content, flavonoid content and total antioxidant activity of the R. anatolyi flowers were 3,99 +/- 0,35 mu mol kg(-1), 346.27 +/- 6.51 mg 100 g(-1), 21.94 +/- 0.37 mg gallic acid g(-1), 5.60 +/- 0.13 mg quercetin g(-1) ve 29.65 +/- 0.26 mM ascorbic acid g(-1), respectively. Consequently, the mineral, trace element, vitamin E and vitamin C, total phenol and flavonoid levels in the R. anatolyi flowers were high, and the R. anatolyi flowers methanol extract effectively inhibited free radicals. From this point of view, it is thought that it can be used in the preventive treatment of many diseases that may be caused by free radical species and that these data will be a reference for other studies.
Comparison of Xanthine Oxidase Inhibitory Activities and Phenolic, Fatty Acid, Element, and Vitamin Levels of Four Mushroom Species
(Wiley, 2025) Ekin, Suat; Akkoyun, Mahire Bayramoglu; Bakir, Ahmet; Akcay, Mustafa Emre; Ekin, Emre Can
The purpose of this study was to determine the antioxidant properties and the composition of phenolic compounds, trace elements, vitamins, and fatty acids in the edible mushrooms Helvella leucopus, Tricholoma terreum, Lepista nuda, and Marasmius oreades, as well as their inhibitory effect on xanthine oxidase. Elemental analyses were conducted using ICP-OES. Phenolic compounds and vitamins were performed by HPLC, while identification of fatty acids was performed by GC-MS. HPLC analysis revealed the phenolic compounds in H. leucopus, T. terreum, L. nuda, and M. oreades, with gallic acid being the main compound identified, with levels of 133.04, 246.49, 408.64, and 129.302 mu g/g dry weight, respectively. For vitamins, alpha-tocopherol is the most primary vitamin found with values of 0.6009, 0.79, and 0.3581 mu mol/kg dw. GC-MS analysis determined that the fatty acids with linoleic acid are the major fatty acids identified, with percentages of 30.82%, 19.92%, 30.38%, and 20.86%. ICP-OES measurement indicated that trace elements with iron as the dominant trace element were observed, with concentrations of 0.248, 0.237, 0.449, and 0.1998 mmol/kg dw. The XO inhibitory activities of four mushrooms were assayed, with the IC50 values of 39.97, 20.71, 11.71, and 23.85 mu g/mL, respectively. L. nuda and T. terreum may be effective for hyperuricemia and gout, which is associated with the results of phenolic compounds, some vitamins, trace elements, and linoleic acid contents on the inhibitory activities against xanthine oxidase. The results of medicinal mushrooms have shown that they could potentially be useful as inhibitors for the prevention of XO-related diseases induced by ROS.
Evaluation of the Protective Effect of Chlorogenic Acid and Rhabdosciadium Anatolyi Against Cyclophosphamide-Induced Ovarian Toxicity in the Rat With Histopathological and Immunohistochemical Findings
(Kafkas Univ, veteriner Fakultesi dergisi, 2020) Alkis, Ismet; Ekin, Suat; Yildirim, Serkan; Bakir, Ahmet; Eser, Gizem; Firat, Mehmet
Cyclophosphamide (CD) has a range of adverse effects on ovarian tissue in humans. It is widely an anticancer drug and used in autoimmune disorders. Also, CD produces reactive oxygen species. In the current study, we evaluated possible protective roles of Rhabdosciadium anatolyi (RA) and chlorogenic acid (CA) on histopathological alterations and immunohistochemical assessment of 8-OHdG in the ovarian tissues of female rats given cyclophosphamide. Female Wistar albino rats were divided into six experimental groups, each consisted of eight rats: control group, CD (200 mg/kg) with i.p. for the first day as single dose, CA (100 mg/kg), RA (300 mg/kg) for each day, RA (300 mg/kg) + CD (200 mg/kg) and CA(100 mg/kg) + CD (200 mg/kg) groups. The rats were administered treatments lasted 7 days for all groups. CA and RA treatment is associated with positive the ovary action CD-induced ovotoxicity in rats. CA and RA could ameliorate the histopathological and immunohistochemical finding restoring which may show moderate levels of primordial follicle, primary, secondary, tertiary and graafian follicles were observed degeneration in germinative cells.Therefore, our results suggest that chlorogenic acid and Rhabdosciadium anatolyi might be a protective effect for CD-induced ovotoxicity.
Protective Effect of Momordica Charantia Against Hepatorenal Toxicity Induced by Potassium Bromate (KBrO3) in Rats
(Univ Zulia, Facultad Ciencias veterinarias, 2025) Karagozoglu, Fatma; Uyar, Ahmet; Akkoyun, Hurrem Turan; Altun, Serdar; Akkoyun, Mahire Bayramoglu; Aydin, Sevinc; Bakir, Ahmet
This study aims to evaluate the effects of Momordica charantia on hepatorenal toxicity caused by potassium bromate (KBrO3) in rats. Wistar rats were divided into 4 groups as control, KBrO3, bitter melon (MC), and KBrO3+MC. Examining the antioxidant enzyme grade of the kidney tissues, it was found that the enzyme activities of catalase (CAT) (P<0.05), glutathione peroxidase (GSH-Px) (P<0.01), and superoxide dismutase (SOD) (P<0.01) decreased in the KBrO3 group in comparison to the control. There was a significant decrease (P<0.001) in glutathione (GSH) levels and an increase (P<0.01) in malondialdehyde (MDA) level in the KBrO3 group in comparison to the control. Examining the antioxidant enzyme activities in liver tissue, it was determined that CAT, GSH-Px, and SOD enzymes reduced significantly (P<0.05, P<0.01, and P<0.001, respectively) in the KBrO3 group in comparison to the control, and the enzyme activity of decreased CAT, GSH-Px, and SOD enzymes significantly elevated (P<0.01) in the MC group. There was a reduction in GSH level in the KBrO3 group in comparison to the control (P<0.01), while an increase was recorded in the KBrO3+MC group (P<0.05). MDA level in liver tissue increased in KBrO3 group in comparison to the control (P<0.01) and MC decreased the MDA level. Histopathological analysis results indicate severe degenerative and necrotic lesions in hepatorenal histoarchitecture of KBrO3 rats in comparison to the control. However, application of MC+KBrO3 significantly reduced the induced hepatorenal injury with a concomitant increase in histopathological lesions. From the immunohistochemical aspect, MC revealed apoptosis concomitant with the suppression of necrosis in the KBrO3-treated rats as demonstrated by the caspase-3 activity.
The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats
(Marmara Univ, inst Health Sciences, 2022) Bakir, Ahmet; Ekin, Suat; Oztas, Sevgi; Oto, Gokhan
Objective: This study was designed to examine the potential protective effects of Rheum ribes L., and quercetin on protein carbonyl (PCO) in kidney and liver tissue, trace elements (Fe, Cu, Zn) and mineral (P) in serum samples in Wistar rats of carbon tetrachloride (CCl4)-induced oxidative damage.Methods: The 2, 4-dinitrophenylhydrazine (DNPH) method is the most reliable method widely used to measure carbonyl levels in proteins. In this study, the effect of Rheum ribes L. (Rr) and quercetin on protein carbonyl, trace elements (Fe, Cu, Zn) and mineral (P) levels against carbon tetrachloride (CCl4) mediated liver and kidney damage was investigated. For this purpose, 56 Wistar albino female rats weighing 200 +/- 220 g were used. Groups were designed as: controls, 0.3 ml DMSO, 1 ml/kg olive oil, 1 ml/kg CCl4, 100 mg/kg Rr, 100 mg/kg quercetin, 100 mg/kg Rr+1 ml/kg CCl4 and 100 mg/kg quercetin+1 ml/kg CCl4 groups.Results: The results showed that the CCl4 group had significantly higher level of protein carbonyl (PCO) than the control, DMSO, olive oil, Rr and quercetin groups (p<0.001, p<0.05, p<0.001, p<0.01, and p<0.01, respectively). A significant elevation in the group of CCl4 + quercetin, compare to control, DMSO, olive oil, Rr and quercetin groups (p<0.001, p<0.05, p<0.001, p<0.01, and p<0.05, respectively) in the liver tissue. Additionally, the CCl4 group had significantly higher level of PCO than the control, DMSO, olive oil, Rr and quercetin groups (p<0.001, p<0.01, p<0.001, p<0.001, and p<0.001, respectively). Similarly, the CCl4 + Quercetin group had increased level of PCO compared to the control and Rr.groups (p<0.05 and p<0.05) in the kidney tissue.Conclusion: In the study, it was seen that the bioactive substances in Rheum ribes L. (root) and quercetin, a standard antioxidant, could be an alternative against the toxic effect of CCl4.
Protective Effects of Saffron, Safranal and Crocin Administration on Vitamins (A, D, E, K) and Protein Carbonyl Levels Against Cci4-Induced Oxidative Damage in Rats
(Marmara Univ, Fac Medicine, 2024) Bakir, Ahmet; Yildiz, Damla; Ekin, Suat; Oto, Gokhan; Aras, Ibrahim; Bayram, Irfan
Objective: The possible effects of saffron and its active components on oxidative stress are known. Protein carbonyls (PCO), formed due to protein exposure to oxidizing agents, are a newly researched topic. In this study, it was aimed to determine, antioxidant fatsoluble vitamins (A, D, E, K) and PCO values after saffron, safranal and crocin administration with carbon tetrachloride (CCl4) in rats. Materials and Methods: Fifty-four Wistar albino male rats were randomly selected, and 9 groups of n=6 were formed. Vitamin levels in rat serum were determined by HPLC and PCO levels were determined by spectrophotometric method. Results: A significant difference (p<0.01) was found between the CCl4 and the saffron, safranal and crocin groups. A significant decrease was observed in retinol and cholecalciferol values between CCl4 and saffron group (p<0.05, p<0.001), and a significant decrease in cholecalciferol and phylloquinone levels between CCl4 and safranal groups (p<0.01, p<0.05). Moreover, a decrease in cholecalciferol level (p<0.05) was determined between the olive oil, saffron and CCl4+crocin groups. Conclusion: As a result, saffron and safranal have a protective effect against CCl4-induced oxidative damage to PCO, retinol, phylloquinone and cholecalciferol, and this effect may be due to the potent antioxidative effects of saffron and safranal.
Total Sialic Acid, Antioxidant Enzyme Activities, Trace Elements, and Vitamin Status Before and After Surgery in Women With Uterine Myoma and Endometrial Cancer
(Springer Heidelberg, 2023) Gok, Fazilet; Ekin, Suat; Karaman, Erbil; Erten, Remzi; Yildiz, Damla; Bakir, Ahmet
The objective of present study was to examine endometrial tissue Be, As, Cr, Mo, Sr, Ti, Tl, Cu, Co, Se, Zn, Mn, Fe, Cd, Pb, Mg, P, erythrocytes CAT, SOD, GSH-Px, GSH, MDA, serum retinol, cholecalciferol, phylloquinone, TSA, LSA, TOS, and TAS status and to evaluate the relationships between the variables. The study had 110 participants; of these, 50 were women with uterine myoma (UM), 10 were women with endometrial cancer (EC), and 50 were healthy female subjects. In the study, vitamin analyses by HPLC and element analyses were determined using ICP-OES method. It was observed that EC group was significantly lower than healthy group in terms of levels of cholecalciferol (p < 0.05), phylloquinone (p < 0.01), GSH (p < 0.05), Fe (p < 0.05), and had a significant rise in Mg/Fe (p < 0.01) and Zn/Fe (p < 0.05) in preoperative period. UM group had significantly lower retinol (p < 0.05), phylloquinone (p < 0.001), GSH-Px (p < 0.01), GSH (p < 0.01), Cr (p < 0.01), Cu (p < 0.05), Mg (p < 0.01), and Zn (p < 0.01) levels than control group in preoperative period and significantly higher levels of MDA (p < 0.01), TSA (p < 0.01), and LSA (p < 0.01) than control group. It was found that significant associations were observed between Cu-CA 15-3 (r = 0.558, p = 0.016), Mn-CA 15-3 (r = 0.511, p = 0.030), P-CA 15-3(r = - 0.502, p = 0.034) and with UM, also between GSH-CA-125 (r = - 0.825, p = 0.022) and with EC group. The results of correlation analysis observed that concentrations of Cu, Mn, P, and GSH together with CA 15-3 and CA-125 levels might be important for monitoring patients with UM and EC before surgery.