Browsing by Author "Basbugan, Y."

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Activity, Acute and Sub-Acute Toxicity and Safety Assesment of the Hydroalcholic Root Extract of Diplotaenia Turcica
(Agricultural Research Communication Centre, 2018) Ozdek, U.; Basbugan, Y.; Yildirim, S.; Boga, M.; Firat, M.; Deger, Y.
Diplotaenia turcica is widely utilized in conventional treatment in the east of Turkey. Due to the insufficient data on the safety profile, the acute and sub acute toxicity of Diplotaenia turcica was determined. Furthermore, total phenolic content, flavonoid content and antioxidant activity of the extract were determined. The acute toxicity of hydroalcoholic root extract of Diplotaenia turcica (HREDT) was evaluated in mice after administration of single oral dose at the 5000 mg/kg (acute model) and rat after 28 days orally administration at the dose range of 250, 500 and 1000 mg/kg (subacute model). According to result, the LD50 value of HREDT was found to be greater than 5000 ing/kg. In sub acute toxicity study, no statistically significant differences were observed in the values of hematological and pathological parameters in comparison with control group. It was determined that the glucose, triglyceride, cholesterol and LDL levels exerted a significant effect depending on the HREDT doses. The results from the present study found out that HREDT did not produce any toxic effects or deaths in animals for both single and chronic administration. Additionally, HREDT showed moderate antioxidant activities and exhibited relatively notable total phenolic content.
Amiodarone's Effects on Pathological, Hematological and Biochemical Parameters in Acute Selenium Poisoning
(Elsevier Ireland Ltd, 2016) Yucel, U. M.; Basbugan, Y.; Uyar, A.; Komuroglu, A. U.; Keles, O.
The Effect of Pde5 Inhibitors on Bone and Oxidative Damage in Ovariectomy-Induced Osteoporosis
(Wiley-blackwell, 2016) Alp, H. H.; Huyut, Z.; Yildirim, S.; Basbugan, Y.; Ediz, L.; Sekeroglu, M. R.
Effects of Sedation Doses of Propofol and Midazolam on Levels of Ngal, Cystatin-C, Kim-1 in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Soyalp, C.; Kömüroğlu, A.U.; Yuzkat, N.; Basbugan, Y.; Tunçdemir, Y.E.
Limited data are available regarding the impact of their sedation doses midazolam and propofol on early biomarkers of acute kidney injury (AKI). This study aimed to investigate the effects of sedation doses propofol and midazolam on early biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL), cystatin C (CyC) and kidney injury molecule 1 (KIM-1) of AKI. A total of 24 Wistar albino rats were separated into three groups (n = 8 per group): a control group (intraperitoneal injecti on [IP] saline injection once daily for 7 days), a propofol group (IP injection of 2.5 mg/kg propofol once daily for 7 days), and a midazolam group (IP injection of 5 mg/kg midazolam once daily for 7 days). For each group, urinalysis (for urea, creatinine, total protein, NGAL, CyC, and KIM-1) was performed on Day 0 and Day 7; serum analysis (for urea, creatinine, total protein, albumin globulin, ALT, AST, NGAL, CyC, and KIM-1) was performed on day 7. No significant difference was noted between control, propofol and midazolam groups in terms of Day 7 serum KIM-1, CyC, and NGAL levels and Day 0 and Day 7 urinalysis findings (KIM-1, CyC, NGAL, urea, and creatinine levels). DISCUSSION AND CONCLUSION: The findings revealed a similar safety profile for seven-day propofol and midazolam administration in rats in terms of the traditional (creatinine, urea) and early biomarkers (NGAL, CyC, KIM-1) of AKI. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Effects of Sugammadex on Coagulation in Pregnant Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Keskin, M.E.; Kaplan, H.S.; Gülhas, N.; Bayram, Y.; Basbugan, Y.
Some studies have reported that sugammadex affects coagulation parameters. However, the effects of sugammadex on coagulation in pregnant women have not been adequately evaluated. This study investigated the effects of sugammadex administration on routine coagulation tests and levels of coagulation factors (FII, FV, FVII, FVIII, FIX, and FX) in pregnant rats. After ethics committee approval (2019/06), 24 female Wistar albino rats (n = 12; 19–20 days of gestation; weight 230–380 g) were randomly divided into four groups: control (n = 6), sugammadex (n = 6), pregnant (n = 6), and pregnant sugammadex (n = 6). Ketamine (50 mg/kg) and xylazine (10 mg/kg) were administered intraperitoneally to all groups. Saline (SF) (1 ml/kg) was administered to the control and pregnant groups. Sugammadex was administered to the sugammadex and pregnant sugammadex groups at a dose of 16 mg/kg. Blood samples were collected at 30 min after SF and sugammadex administration in all rats. The levels of PT, INR, aPTT, fibrinogen and coagulation factors were measured in plasma obtained from blood samples. All animals were sacrificed by cervical dislocation after the procedure. The mean weights of the rats were significantly higher in the pregnant groups (pregnant and pregnant sugammadex groups) than in the non-pregnant groups (control and sugammadex groups). The mean PT and INR values were significantly lower in the pregnant than non-pregnant groups. No significant differences were observed in any of the parameters evaluated between the sugammadex-and SF-administered groups. Sugammadex administration did not affect routine coagulation tests or the levels of coagulation factors in pregnant rats. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Seroprevalence of Tularemia in Risk Groups of Humans and Animals in Van, East of Turkey
(Ankara Microbiology Society, 2015) Bayram, Y.; Özkaçmaz, A.; Parlak, M.; Basbugan, Y.; Kiliç, S.; Güdücüoʇlu, H.
Tularemia has become a re-emerging zoonotic disease in Turkey recently. The aims of this study were to determine the seroprevalence of tularemia in humans and their animals living in rural risky areas of our region and to investigate the risk factors. Between January and July 2012, people living in rural areas of Van province (located at eastern part of Turkey) and their domestic animals were included in the study. The sample size was determined by using cluster sampling method like in an event with known prevalence and planned as a cross-sectional epidemiological study. Proportional random sampling method was used to determine which individuals will be included in the study. Presence of tularemia antibodies in the sera of a total 495 voluntary persons (343 female, 152 male; age range: 18-79 years, mean age: 40.61) and their 171 animals (40 cattle, 124 sheep and 7 goats) were screened by microagglutination test using safranin O-stained F.tularensis antigen (Public Health Agency of Turkey). For the evaluation of cross-reactivity between Brucella spp., tularemia positive serum samples were also tested with brucella microagglutination test. Among human and animal samples, 11.9% (59/495) and 44% (76/171) yielded positive results with the titers of ≥ 1:20 in F.tularensis microagglutination test, respectively. However, 69.5% (41 /59) of human sera and 78.9% (60/76) of animal sera demonstrated equal or higher titers in the brucella test, so those sera were considered as cross-reactive. After exclusion of these sera, the seroprevalence for F.tularensis were calculated as 3.6% (18/495) for humans and 9.4% (16/171) for animals. Among the 16 animals with positive results, 12 were sheep, three were cattle and one was goat. The difference between seropositivity rates among the domestic animal species was not statistically significant (p< 0.05). In addition, no statistically significant differences were found between risk factors including insect bite, tick bite, contact with rodents, eating the meat of hunted animals (rabbit), having pet (cat) in home (p> 0.05). In this study, the rate of tularemia seropositivity among humans was similar to the results of previous studies which were performed in our country; however the seropositivity rate of tularemia among domestic animals in our study was higher than the results of a few studies which were conducted on domestic animals. In conclusion, preventive procedures and precautions must be taken into consideration to control the transmission of the infection.
Serum Potassium-Lowering Effects of Insulin Plus Dextrose and Adrenalin Treatment That Enhance Intracellular Potassium Transitions in Hyperkalemic Diarrheic Calves
(Univ Agriculture, Fac veterinary Science, 2016) Altug, N.; Yuksek, N.; Ozkan, C.; Basbugan, Y.; Keles, I.; Agaoglu, Z. T.; Akgul, Y.
The study examined the serum potassium-lowering effects of different treatment options for hyperkalemia in diarrheic calves (n=18). Calves were allocated into three treatment groups. All groups received isotonic sodium bicarbonate solution for a period of 90 minutes. The animals in group 1 received no further treatment, those in group 2 received a further dose of 50% dextrose and insulin, and those in group 3 received a further dose of adrenaline. High serum K levels in all groups significantly decreased after treatment (AT) than before treatment (BT). Serum potassium levels AT in groups 2 and 3 were found to be significantly lower than those in group 1. Increase in pH, bicarbonate and total carbon dioxide values were observed AT in all groups. However, bicarbonate and total carbon dioxide levels in the AT were not statistically significantly different from those in the adrenaline group compared with the groups 1. These parameters were significantly lower in the group 2. Moreover, the concentration of glucose in the groups 2 in contrast to the adrenalin group was higher than that in both the BT and group 1 AT. The insulin + dextrose combination and adrenaline applications in hyperkalemic calves had similar effects. However, the administration of adrenaline had a lower negative effect on metabolic acidosis treatment. (C) 2015 PVJ. All rights reserved