Browsing by Author "Battal, A."

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Exploring the Protective Effects of Chinar (Platanus Orientalis L.) Leaf on Testes Damage Induced by Ethanol in Rat Model
(Pakistan Agricultural Scientists Forum, 2024) Yaman, T.; Dogan, A.; Keles, O. F.; Donmez, F.; Battal, A.; Anuk, O. O.
The aim of this study was to investigate the protective effects of an infusion obtained from dried Chinar (Platanus orientalis L.; PO) leaves against ethanol (EtOH)-induced damage in rat testes. Thirty male Wistar rats were randomly and equally divided into 5 groups, as the Control, EtOH 20%, EtOH 20% + Silymarin (10 mg/kg/day), EtOH 20% + PO20 mg/mL infusion, and EtOH 20% + PO -60 mg/mL infusion. Histopathologically, treatment with PO -60 leaf infusion resulted in improvements in EtOH-induced damage in the testes by inhibiting the depletion of germ cells and loss of spermatozoa. Immunohistochemically, an increase in the expression of endothelial nitric oxide synthase (eNOS) and caspase-3 was observed in the EtOH group. Treatment with the PO extract markedly reduced the EtOH-induced expression of eNOS and caspase-3 in the EtOH 20% + PO -60 group. According to the biochemical results, both of the PO infusion treatments caused a decrease in the malondialdehyde levels compared to the EtOH group. On the other hand, the glutathione contents in the treatment groups were significantly higher than ethanol group. Fluctuations in antioxidant defense enzyme activities were determined. PO leaf might have a protective role against EtOH-induced testicular damage in rats by inducing apoptosis via the upregulation of eNOS expression and preventing lipid peroxidation.
Horse Mushroom (Agaricus Arvensis Schaeff.) Prevents Oxidative Stress in Carbon Tetrachloride Toxicity
(Elsevier, 2020) Dogan, A.; Ilhan, M.; Battal, A.
Environmental pollutants cause severe oxidative stress and lipid peroxidation. Oxidative stress generates free radicals (FRs) such as peroxyl radicals, superoxide radicals, hydroxyl radicals, etc. These FRs suppress in vivo and in vitro antioxidant defense systems in living systems. Bioactive compounds present in mushrooms have been reported to increase the activity of antioxidant enzymes and suppress the formation of possible FR sources. Agaricus arvensis is a macrofungus known as horse mushroom. Some studies report the antioxidant activity of A. arvensis. For instance, administration of the extract obtained from A. arvensis had regulative effects in liver enzymes and antioxidant parameters in kidney, liver, and brain tissues in CCl4-administered rats. A similar study showed that A. arvensis extract reduced erythrocyte fragility, and it regulated many hematological parameters in CCl4-administered rats. Therefore, the present study aimed to collect some data about oxidative stress, FRs, antioxidant activity, and mechanism of A. arvensis. © 2021 Elsevier Inc. All rights reserved.
Particle Bombardment Transformation of Some Turkish Wheat Cultivars With Tanac69-1 and Ttnamb2 Genes
(Elsevier Science Bv, 2012) Battal, A.; Baloglu, M. C.; Kavas, M.; Yucel, M.; Oktem, H. A.
Urtica Dioica L
(Springer International Publishing, 2022) Dogan, A.; Dönmez, F.; Battal, A.
Urtica dioica L., known as nettle, is a herbaceous, perennial plant, belonging to Urticaceae family. This chapter covers description and distribution of the plant. Additionally, its chemical composition and traditional use were presented in detail. The in vitro and in vivo activities of nettle extracts (root, leave, and seed) such as antimicrobial, antiviral, antioxidant, anti-inflammatory, antidiabetic, hepatoprotective, hypotensive, and anticancer have been highlighted via its rich phytochemical composition. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.
Vector Construction Strategies for Transformation of Wheat Plant
(Pakistan Agricultural Scientists Forum, 2013) Baloglu, M. C.; Battal, A.; Aydin, G.; Eroglu, A.; Oz, M. T.; Kavas, M.; Yucel, M.
Gene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1 and TtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant.