Browsing by Author "Battal, Abdulhamit"

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Ameliorative Effects of Scutellaria Pinnatifida Subsp. Pichleri (Stapf) Rech.f. Extract in Streptozotocin-Induced Diabetic Rats: Chemical Composition, Biochemical and Histopathological Evaluation
(Bmc, 2023) Bakac, Mehmet Salih; Dogan, Abdulahad; Yilmaz, Mustafa Abdullah; Altindag, Fikret; Donmez, Fatih; Battal, Abdulhamit
BackgroundsScutellaria Pinnatifida subsp. pichleri (Stapf) Rech.f. (SP) is used in folk medicine for the treatment of diabetes. The aim of the study was to determine the phenolic profile of SP extract (SPE) by LC-MS/MS and to investigate the antidiabetic, hepatoprotective and nephroprotective effects of SPE in streptozotosin (STZ)-induced diabetic rat model.MethodsForty-two rats were randomly divided into six groups (n = 7): Control (nondiabetic), diabetes mellitus (DM), DM + SP-100 (diabetic rats treated with SPE, 100 mg/kg/day), DM + SP-200 (diabetic rats treated with SPE, 200 mg/kg/day), DM + SP-400 (diabetic rats treated with SPE, 400 mg/kg/day) and DM + Gly-3 (diabetic rats treated with glibenclamide, 3 mg/kg/day). Live body weight, fasting blood glucose (FBG) level, antidiabetic, serum biochemical and lipid profile parameters, antioxidant defense system, malondyaldehyde (MDA) and histopathological examinations in liver, kidney and pancreas were evaluated.ResultsApigenin, luteolin, quinic acid, cosmosiin and epigallocatechin were determined to be the major phenolic compounds in the SPE. Administration of the highest dose of SP extract (400 mg/kg) resulted in a significant reduction in FBG levels and glycosylated hemoglobin levels in STZ-induced diabetic rats, indicating an antihyperglycemic effect. SPE (200 and 400 mg/kg) and glibenclamide significantly improved MDA in liver and kidney tissues. In addition, SPE contributed to the struggle against STZ-induced oxidative stress by stimulating antioxidant defense systems. STZ induction negatively affected liver, kidney and pancreas tissues according to histopathological findings. Treatment with 400 mg/kg and glibenclamide attenuated these negative effects.ConclusionsIn conclusion, the extract of the aerial part of Scutellaria pinnatifida subsp. pichleri has hepatoprotective, nephroprotective and insulin secretion stimulating effects against STZ-induced diabetes and its complications due to its antidiabetic and antioxidant phytochemicals such as apigenin, luteolin, quinic acid, cosmosiin and epigallocatechin.
The Effects of Cadmium Concentrations on Germination and Physiological Parameters in Tomato (Solanum Lycopersicum Lam.)
(2023) Bingöl, Ömer; Battal, Abdulhamit; Erez, Mehmet Emre
Cadmium (Cd) is omnipresent trace element in environmental that is unessential in plants. Cd levels rise because of anthropogenic activity such as the combustion of fossil fuels, phosphate fertilizer manufacturing, mineral fertilizers, batteries technology. It is extremely toxic metal and reduces plant growth. In this context, the aim of this study was to investigate the effect of different concentrations (5/10/20/40 ppm) of Cd on germination of seeds and physiological effects in early developmental stage of tomato Solanum lycopersicum Lam. seedlings. 20 ppm (80%) and 40 ppm (83.3%) Cd concentrations caused significantly decrease in germination percentage. All Cd treatments were resulted with decrease in Vigor Index, especially in 20 ppm (42% decrease compared to control). Application of 5 ppm Cd caused decreases in chlorophyll and carotenoid contents in seedlings. Finally, significant decrease in protein content of 5 ppm, 10 ppm and 20 ppm treated seedlings were determined compared to control. As a conclusion, Cd negatively affected germination and physiological parameters of tomato in early developmental stage. Overall, these results indicate that Cd affects different physiologic processes and pathways according to concentration.
Evaluation of the in Vitro and in Vivo Effects of the Ethanolic Lyophilized Extract Obtained From Pholiota Aurivella Mushroom on Cci4-Induced Toxicity in Rats
(Parlar Scientific Publications (p S P), 2019) Dogan, Abdulahad; Dalar, Abdullah; Battal, Abdulhamit; Uyar, Ahmet; Sadullahoglu, Cemil; Celik, Ismail; Uzun, Yusuf
The aim of this study was to evaluate Pholiota aurivella extract's chemical and mineral composition, total antioxidant capacity, histopathological changes in liver and kidney, as well as its possible protective/harmful role against carbon tetrachloride (CCl4)-induced oxidative stress in rats. After the toxicity test, twenty four rats were divided into four experimental groups: Control, CCl4, CCl4+P. aurivella (100 mg/kg, extract) and CCl4+P.aurivella (500 mg/kg, extract) groups. Some biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) in serum, malondialdehyde (MDA) and antioxidant defense system (ADS) constituents such as reduced glutathione (GSH) levels, glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities in the liver, brain and kidney tissues, histopathological changes in liver and kidney of rats were observed. P.aurivella extract was rich in phenolic (p-coumaric and protocatechuic acids) and fatty acid (linoleic, oleic and palmitic acids) compounds and high level of Arsenic. In vivo studies revealed that P.aurivella extract suppressed the levels of ADS enzymes and increased the MDA content and AST, ALT and LDH in treated groups. According to histopathological investigation, liver and kidney revealed severe histopathological changes both in CCl4 group and P.aurivella- treated groups. These results have shown that oral administration of P. aurivella extract caused a significant toxic effects in rats. Therefore, in the context of the data obtained from this study the P. aurivella could not be regarded as a safe food source.
Exploring of the Ameliorative Effects of Ethanolic Extract of the Wheatgrass (Triticumaestivum L.) on Surgically Induced Endometriosis Rat Model
(Soc Stiinte Farmaceutice Romania, 2023) Adiyaman, Abdullah; Battal, Abdulhamit; Ilhan, Mert
The purpose of this study was to investigate the healing effects of an ethanolic wheatgrass (WG) extract obtained from young wheat leaves on a surgically induced endometriosis rat model. 30 female Wistar albino rats with endometriosis were randomly organized into five groups (N = 6): Control, Letrozole a reference drug for endometriosis (0.2 mg/kg/day), WG-25 (25 mg/kg/day WG extract), WG-100 (100 mg/kg/day WG extract) and WG-400 (400 mg/kg/day WG extract). WG-100 extract and Letrozole caused a significantly decrease in adhesion score compared to the control. Additionally, treatment with WG extracts resulted in a significant reduction of endometrial cyst volumes compared to the control group. IL-6 cytokine levels in peritoneal fluid of WG-100 and Letrozole groups were importantly lower than the control. Matrix metallopeptidase 9, VEGF and kinase insert domain receptor genes were expressed in endometrial cysts. According to LC-MS/MS analysis, aconitic acid was the major phenolic acid (54.004 mg analyte/g extract) in the WG extract. Additionally, quinic acid (3.687 mg analyte/g extract) and protocatechuic acid (0.014 mg analyte/g extract) were determined in the WG extract. Moreover, the WG extract had vitamin E (5.49 +/- 0.08 mg/g extract) and vitamin C (7.42 +/- 0.13 mg/g extract). As conclusion, WG extract obtained from young leaves of wheat has ameliorative effects on surgically induced endometriosis due to its rich phytochemical content.
Exploring of the Ameliorative Effects of Nerium (Nerium Oleander L.) Ethanolic Flower Extract in Streptozotocin Induced Diabetic Rats Via Biochemical, Histological and Molecular Aspects
(Springer, 2023) Battal, Abdulhamit; Dogan, Abdulahad; Uyar, Ahmet; Demir, Abdulbaki; Keles, oemer Faruk; Celik, Ismail; Aslan, Ali
Background Nerium oleander L. is ethnopharmacologically used for diabetes. Our aim was to investigate the ameliorative effects of ethanolic Nerium flower extract (NFE) in STZ-induced diabetic rats.Methods Seven random groups including control group, NFE group (50 mg/kg), diabetic group, glibenclamide group and NFE treated groups (25 mg/kg, 75 mg/kg, and 225 mg/kg) were composed of forty-nine rats. Blood glucose level, glycated hemoglobin (HbA1c), insulin level, liver damage parameters and lipid profile parameters were investigated. Antioxidant defense system enzyme activities and reduced glutathione (GSH) and malondialdehyde (MDA) contents and immunotoxic and neurotoxic parameters were determined in liver tissue. Additionally, the ameliorative effects of NFE were histopathologically examined in liver. mRNA levels of SLC2A2 gene encoding glucose transporter 2 protein were measured by quantitative real time PCR.Results NFE caused decrease in glucose level and HbA1c and increase in insulin and C-peptide levels. Additionally, NFE improved liver damage biomarkers and lipid profile parameters in serum. Moreover, lipid peroxidation was prevented and antioxidant enzyme activities in liver were regulated by NFE treatment. Furthermore, anti-immunotoxic and anti-neurotoxic effects of NFE were determined in liver tissue of diabetic rats. Histopathogically, significant liver damages were observed in the diabetic rats. Histopathological changes were decreased partially in the 225 mg/kg NFE treated group. SLC2A2 gene expression in liver of diabetic rats significantly reduced compared to healthy rats and NFE treatment (25 mg/kg) caused increase in gene expression.Conclusion Flower extract of Nerium plant may have an antidiabetic potential due to its high phytochemical content. [Graphics] .
Exploring Phytochemical Constituents of Achillea Arabica Kotschy. Ethanolic Flower Extract by Lc-ms/Ms and Its Possible Antioxidant and Antidiabetic Effects in Diabetic Rats
(Walter de Gruyter Gmbh, 2023) Hanalp, Hanife Ceren; Dogan, Abdulahad; Saygi, Tuba Kusman; Donmez, Fatih; Battal, Abdulhamit
The aim of this study was to reveal the antidiabetic and antioxidant effects of ethanolic lyophilized extract of Achillea arabica flower extract against streptozotosine (STZ)-induced in diabetic rats and to determine its phytochemical content by liquid chromatography with tandem mass spectrometry (LC-MS/MS). After toxicity test, 35 female rats were divided into five groups. Control, diabetes mellitus (DM), A.arabica (400 mg/kg) extract, DM + A. arabica (400 mg/kg) extract and DM + Glibenclamide (2 mg/kg). It was determined that while diabetic rats treated A.arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels. According to LC-MS/MS results of A. arabica flower extract, quinic acid (2439.9 mu g/g), cyranoside (858.4 mu g/g), chlorogenic acid (698.7 mu g/g), and cosmosiin (347.8 mu g/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this extract according to nuclear magnetic resonance (NMR) and Mass analyses and these compounds were named edremitine and achillosine, respectively. Thus, A.arabica flower extract has possible therapeutic effects to prevent high blood glucose level and oxidative stress caused by DM in liver and kidney via its high phenolic content.
Exploring the Ameliorative Effects of Hypericum Scabrum L. on a Surgically-Induced Endometriosis Rat Model and Its Phytochemical Lc-ms/Ms- Profile
(Soc Stiinte Farmaceutice Romania, 2023) Ilhan, Mert; Battal, Abdulhamit; Kaptaner, Burak; Dogan, Abdulahad; Donmez, Fatih; Yilmaz, Mustafa abdullah; Eroglu, Huseyin
Endometriosis is described as the existence and expansion of functional endometrial-like tissues in another part of the uterine cavity. It affects not only postmenopausal women but also teenagers after menarche, causing subfertility, dysmenorrhea and severe pelvic pain. Since no specific treatment is acknowledged for this disease, the present study aimed to investigate the efficacy of H. scabrum in a surgically-induced endometriosis rat model. In order to test the efficacy of H. scabrum on endometriosis, n-hexane, ethyl acetate (EtOAc), methanol (MeOH) and aqueous extracts were prepared from the aerial parts of H. scabrum. The cytokine levels, antioxidant enzyme activities, the contents of malondialdehyde (MDA) and glutathione (GSH) and histopathological analyses were evaluated. In order to reveal the potential active compounds, liquid chromatography-mass/mass spectroscopy (LC-MS/MS) analysis was performed on the MeOH extract who presentedthe highest activity. The MeOH extract significantly decreased the adhesion scores, endometriotic implant volumes, cytokine levels and the antioxidant parameters. Furthermore, histological procedures supported those findings. According to LC-MS/MS analysis of the MeOH extract, the major compounds were mostly phenolic acids and flavonoids. We concluded that the MeOH extract from H. scabrum aerial parts could be used for the treatment of endometriosis, and its activity could be attributed to the chlorogenic acid and quercetin, detected in the highest amounts.
Gene Editing and Mutagenesis Reveal Inter-Cultivar Differences and Additivity in the Contribution of Tagw2homoeologues To Grain Size and Weight in Wheat
(Springer, 2018) Wang, Wei; Simmonds, James; Pan, Qianli; Davidson, Dwight; He, Fei; Battal, Abdulhamit; Akhunov, Eduard
Key messageCRISPR-Cas9-based genome editing and EMS mutagenesis revealed inter-cultivar differences and additivity in the contribution of TaGW2 homoeologues to grain size and weight in wheat.AbstractThe TaGW2 gene homoeologues have been reported to be negative regulators of grain size (GS) and thousand grain weight (TGW) in wheat. However, the contribution of each homoeologue to trait variation among different wheat cultivars is not well documented. We used the CRISPR-Cas9 system and TILLING to mutagenize each homoeologous gene copy in cultivars Bobwhite and Paragon, respectively. Plants carrying single-copy nonsense mutations in different genomes showed different levels of GS/TGW increase, with TGW increasing by an average of 5.5% (edited lines) and 5.3% (TILLING mutants). In any combination, the double homoeologue mutants showed higher phenotypic effects than the respective single-genome mutants. The double mutants had on average 12.1% (edited) and 10.5% (TILLING) higher TGW with respect to wild-type lines. The highest increase in GS and TGW was shown for triple mutants of both cultivars, with increases in 16.3% (edited) and 20.7% (TILLING) in TGW. The additive effects of the TaGW2 homoeologues were also demonstrated by the negative correlation between the functional gene copy number and GS/TGW in Bobwhite mutants and an F-2 population. The highest single-genome increases in GS and TGW in Paragon and Bobwhite were obtained by mutations in the B and D genomes, respectively. These inter-cultivar differences in the phenotypic effects between the TaGW2 gene homoeologues coincide with inter-cultivar differences in the homoeologue expression levels. These results indicate that GS/TGW variation in wheat can be modulated by the dosage of homoeologous genes with inter-cultivar differences in the magnitude of the individual homoeologue effects.
In Vitro Regeneration of Hollyhock Plant, Investigation of Phytochemical Content and Antioxidant Capacity at Different Vegetation Periods and Stress Conditions
(2019) Görmez, Gül; Türker, Musa; Battal, Abdulhamit
Deve gülü bitkisi (Alcea spp), halk arasında tonsilit, mide ve bağırsak ülseri, pnömoni, üriner sistem enfeksiyonları ve allopesi gibi çeşitli hastalıkların tedavisinde kullanılmaktadır. Deve gülü bitkisiyle ilk kez yapılan bu çalışmayla, in vitro rejenerasyonu, farklı vejetasyon dönemlerinde ve stres şartlarında fitokimyasal içeriği ve antioksidan kapasitesi incelenmiştir. Bitkinin tohum dormansisi ve kontaminasyonu engellenerek tohumlar % 86.67 oranında çimlendirilmiştir. Doku kültüründe en iyi kallus oluşumu 14 günlük kotiledonlardan 2mg/l 2,4 D içeren MS elde edilmiştir. Nod ve sürgün ucundan %100 oranında doğrudan rejenerasyon başarılmıştır. En yüksek FCR, FRAP ve ORAC değerleri Temmuz ayına ait infüzyon örneklerinde sırasıyla 16.538±0.019 GA.Eq. mg/gdrog, 225.691 µmol Fe+2/gdrog, 311.86±1.006 µmol TE/gdrog olarak tespit edilmiştir. En yüksek gallik ve klorojenik asit miktarları infüzyonu yapılan Temmuz (meyve) dönemi örneklerinde sırasıyla 0.779 mg/drog, kafeik asit ise Haziran dönemi örneklerinde 1.256 mg/drog. olarak tespit edilmiştir. Haziran ayında makro ve mikro elementler yüksek olarak belirlenmiştir. Ağır metaller ise vejetasyon dönemlerine göre anlamlı bir farklılık göstermemiştir. Doku kültüründe 0.5 mg/l NAA+0.5 mg/l BAP uygulanan örneklerin FCR, FRAP, ORAC ve fenolik asit miktarlarının doğal ortamdakine göre yaklaşık iki katına çıktığı tespit edilmiştir.
Investigation of Neurotoxic and Immunotoxic Effects of the Chinar (Platanus Orientalis L.) Tree Leaf Infusion Against Ethanol Toxicity in Rats
(2020) Donmez, Fatih; Battal, Abdulhamit; Dogan, Abdulahad; Rae, Iain Jonathan; Anuk, Özgür Ozan
In this study, immunotoxic and neurotoxic effects of leaf infusion of Platanus orientalis L. (PO) were investigated in rats ethanol-induced toxicity. Neurotoxic effects of ethanol were evaluated by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in rat brain tissue. Immunotoxic effects were evaluated by adenosine deaminase (ADA) and myeloperoxidase (MPO) biomarkers in rat liver, lung, erythrocyte and spleen tissues. Thirty male Wistar rats were divided into five groups after toxicity study. The groups are explained as follow: Control, Ethanol 20 %, Ethanol 20 % + Silymarin (10 mg kg-1), Ethanol 20 % + PO-20 mg mL-1 infusion and Ethanol 20 % + PO-60 mg mL-1 infusion. The results showed that AChE and BChE activities statistically decreased in the groups treated with PO leaf infusion. While there was a statistically significant decrease in ADA and MPO activities in liver tissue of groups treated with PO leaf infusion. Also, MPO activity of erythrocyte in ethanol group was significantlly increased according to silymarin and PO-60 groups. On the other hand, there was no statistically significant finding was found in lung and spleen tissues. It was concluded that the infusion prepared from P. orientalis leaves suppressed to ethanol-induced neurotoxicity and immunotoxicity.
The Investigation of the Effect of the Salicylic Acid on the Antioxidant Potential, Vitamin C Content and Dna Protection Activity of Wheatgrass ( Triticum Aestivum L.)
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Battal, Abdulhamit; Demir, Serap
Wheatgrass is considered as a superfood because of its high antioxidant potential and beneficial ingredients. Especially in recent years, wheatgrass juice and its powder have been tested in vivo animal models and clinical studies against some diseases. Salicylic acid (SA) one of the important phytohormones controlling plant growth is used as an exogenous elicitor to increase plant bioactive compounds. The aim of this study was to investigate antioxidant potential, vitamin C content and DNA protection of wheatgrass grown from SA pre-treated seeds. For this purpose, total antioxidant statue, total oxidant statue, oxidative stress index and vitamin C level were determined. Additionally, pUC19 plasmid was incubated with Fenton's solution to determine DNA protection activity of lyophilized wheatgrass extract. Bread wheat caryopses were imbibed in different concentrations of SA for 2 hours. Wheatgrass grown from seeds pre-treated with 10(-8) M SA had significantly higher total antioxidant statue, vitamin C and soluble protein content than control. It was observed that wheatgrass extracts had a DNA protective role against hydroxyl radicals. It was concluded that SA pretreatment of seeds could be a good approach to increase their antioxidant potential, soluble protein content and vitamin C level of plants used as antioxidant sources by people.
The Investigation of the Effects of Wheatgrass Extract on Surgically-Induced Endometriosis Rat Model
(2022) Adıyaman, Abdullah; Battal, Abdulhamit; İlhan, Mert
Çikolata kisti olarak bilinen endometriyoz kadınlar arasında doğurganlığı ciddi ölçüde etkileyen bir hastalıktır. Ekmeklik buğday (Triticum aestivum L.) çiminin (BÇ) klorofil, protein, vitamin ve mineral, ferulik asit, gallik asit, rutin, klorojenik asit gibi birçok fenolik bileşikleri içerdiği rapor edilmiştir. Bu çalışmanın amacı BÇ ekstresinin sıçanlarda cerrahi olarak oluşturulan endometriyoz modelinde iyileştirici etkisinin ve BÇ ekstresinin fenolik içeriğinin araştırılmasıdır. Bu amaç için, Tosunbey buğday çeşidine ait tohumlar su kültüründe 14 gün büyütüldükten sonra %80 etanol kullanılarak BÇ ekstresi hazırlandı. 30 adet sıçan beş gruba ayrıldı: Kontrol (%0.5 karboksi metil selüloz mg/kg/gün), Letrozol (0.2 mg/kg/gün), BÇ-25 (25 mg/kg/gün), BÇ-100 (100 mg/kg/gün) ve BÇ-400 (400 mg/kg/gün). Endometriyoz modeli oluşturulduktan sonra, tedavi öncesi ve sonrası adezyon skorlaması, endometriyotik hacim hesaplaması ve sitokin seviyelerinin interlökin-6 (IL-6), tümör nekroz faktör-α (TNF-α) ve vasküler endotelyal büyüme faktörü (VEGF) ölçümü yapıldı. Ayrıca, VEGF-A, FLK-1, ve MMP-9 genlerinin tedavi sonrası ekspresyon seviyeleri yarı-kantitatif olarak hesaplandı. Hazırlanan ekstrenin fenolik içeriği sıvı kromatografisi-kütle kütle spektrometresi (LC-MS/MS) yöntemiyle belirlendi. Adezyon skorları sonuçlarına göre, tedavi öncesinde gruplar arasında bir farklılık bulunmazken, tedavi sonrasında Letrozol ve BÇ-100 gruplarının Kontrol grubuna göre anlamlı olarak daha az adezyon skoruna sahip olduğu bulundu. Letrozol, BÇ-25, BÇ-100 ve BÇ-400 gruplarının tedavi sonrası endometriyotik hacimlerinin tedavi öncesi hacimlerine oranının Kontrol grubuna göre istatistiksel olarak azaldığı tespit edildi. Yapılan gen ekspresyonu çalışmasında, endometriyotik dokuda VEGF-A, FLK-1 ve MMP-9 genlerinin varlığı tespit edildi. Tedavi sonrasında yapılan analizde FLK-1 gen ekspresyon seviyesinde Letrozol grubu Kontrol grubuna göre anlamlı düşüş olduğu gözlemlendi. Tedavi sonrasında IL-6 düzeyinin Letrozol ve BÇ-100 gruplarında Kontrol grubuna göre anlamlı düşüş olduğu tespit edildi. LC-MS/MS sonuçlarına göre, ekstrenin akonitik asit, kinik asit ve protokateşik asit içeriğine sahip olduğu belirlendi. Buğday çiminin 100 mg/kg/gün dozunun endometriyoz iyileşmesine önemli ölçüde katkı sağlayabileceği sonucuna varıldı. Endometriyoz dokusunun ve geliştiği bölgede bulunan organlardaki biyokimyasal ve moleküler düzeydeki değişikliklerin araştırılması ve bitkide bulunan biyoaktif maddelerin saflaştırılarak iyileştirici etkilerinin araştırılması ileri çalışmalar olarak düşünülebilir. Anahtar Kelimeler: Buğday Çimi, Endometriyoz, Gen Ekspresyonu, LC-MS/MS, Sitokin
The Investigation of the Effects Ofsalicylic Acid on Oxidative Stress Index and Dna Protective Activity of Wheatgrass
(2020) Demir, Serap; Battal, Abdulhamit
Buğday çimi yüksek miktarlarda klorofil, E vitamini ve C vitamini içeriğinden dolayı yüksek antioksidan özelliğe sahip olup bazı hastalıkların (anemi, diyabet, kanser) tedavisinde kullanıldığını belirtilen in vitro ve in vivo çalışmalar mevcuttur. Bu çalışmanın amacı, farklı salisilik asit konsantrasyonları (Kontrol (distile su), SA-2 (10-2 M), SA-4 (10-4 M), SA-6 (10-6 M), SA-8 (10-8 M), SA-10 (10-10 M) ve SA-12 (10-12 M)) kullanılarak şişirilen tohumlardan büyütülen buğday çiminin oksidatif stres indeksine ve DNA koruyucu özelliğine etkilerini araştırmaktır. Bunun yanında, buğdaylar su kültüründe 10 gün büyütüldükten sonra, bazı fizyolojik parametrelere, pigment içeriğine, bazı oksidatif stres metabolitlerine antioksidan savunma enzimlerinin aktivitelerine etkileri araştırıldı. Çimlenme oranlarına göre SA-2 uygulamasının çimlenmeyi engellediği bulundu. SA-12 uygulaması sürgün boyunun kontrole göre uzamasına yol açtı. Salisilik asitin farklı konsantrasyonu uygulamaları kök uzunluğunda artışa sebep olurken, kök yaş ağırlığında da artış olduğu bulundu. SA-8 uygulamasının toplam antioksidan seviyesinde artışa sebep olduğu ve oksidatif stres indeksini düşürdüğü bulundu. Katalaz aktivitesinde SA-6 uygulamasının kontrole göre anlamlı artışa sebep olduğu bulundu. Liyofilize buğday çimi ektresinin pUC19 plazmitini Fenton reaksiyonu ile oluşturulan hidroksil radikallerinin zararlı etkilerinden koruduğu gözlendi. Salisilik asit uygulanan buğday çiminin toplam antioksidan seviyesinin artışına yol açarak oksidatif stres indeksini düşürdüğü ve DNA koruyucu etkiye sahip olabileceği sonucuna ulaşıldı. Anahtar kelimeler: DNA koruyucu etki, Ekmeklik buğday, Oksidatif stres indeksi, Salisilik asit
The Investigation of The Medicinal Potential of Alcea Kurdica Alef. in Nature and Tissue Culture
(Soc Stiinte Farmaceutice Romania, 2022) Gormez, Gul; Battal, Abdulhamit; Dalar, Abdullah; Turker, Musa
Alcea kurdica Alef. (AK) a hollyhock has been used in folk medicine to treat various diseases. Though its extensive utilization in traditional medicine, experimental studies regarding to its phytochemical composition is limited. The aim of this study was to determine the antioxidant capacity and phenolic composition of ethanol extract of AK plant collected from the natural environment in different vegetation periods (June-flowering, July-fruiting, August-seeding) and to control its antioxidant capacity and phenolic composition by in vitro shoot tip culture. AK plant at the flowering period had the highest antioxidant capacity, while the lowest activity was determined at the seeding period according to results. Phenolic acids dominated by gallic, chlorogenic and caffeic acids were determined as the major phenolics. According to in vitro results, the use of 1-naphthylacetic acid (NAA) and 6-benzylaminopurine (BA) alone caused significant decrease in the three phenolic acids content compared to the control. On the other hand, combination of NAA and BA caused significant increase in phenolic acids content compared to the control. Moreover, significant increase in ORAC was observed in AK extract obtained from in vitro Murashige & Skoog (MS) media culture supplemented with NAA and BA combination. 15 min of UV exposure negatively affected the antioxidant capacity and phenolic content.
Investigation of the Phytol Kinase Gene Expression in Bread Wheat (triticum Aestivum L.)
(2021) Kılıç, Mustafa; Battal, Abdulhamit
Buğdayın çimi yüksek oranda antioksidan özelliğe, klorofil moleküllerine ve bazı vitaminlere (özellikle E ve C vitaminleri) sahip olduğundan dolayı oldukça popüler olmuştur. E vitamini sentezinde önemli bir role sahip fitolkinaz enzimi vitamin E 5 (VTE5) geni tarafından üretilmektedir. Bu yolakta yapılan çalışmalar buğdayda E vitamini sentezinin anlaşılması için önemlidir. Bu çalışmanın temel amacı, VTE5 geni karakterize edildikten sonraerken gelişim aşamasında ekmeklik buğdayda (Triticumaestivum L.) gen ekspresyon seviyelerinin veE vitamini içeriklerinin belirlenmesidir. Bunun yanında, bazı pigmentlerin ve C vitamini içeriklerinin araştırılması dahedeflendi. Bu amaç doğrultusunda, bitkiler su kültürü kullanılarak 7, 10 ve 14 gün büyütüldüler. VTE5 geninin 2A, 2B ve 2D kromozomlarında lokalize olduğu belirlendi. Klorofil a, klorofil b, toplam klorofil ve karotenoid içeriğinin 14 gün büyütülen bitkilerde 7 gün büyütülenlere göre anlamlı olarak daha fazla olduğu bulundu.14 günlük gövde ve kök dokularında VTE5 ekspresyonunun 7 günlüklere göre arttığı belirlendi. Gövdelerin köklerden daha yüksek E vitamini içeriğine sahip olmasının yanında, 14 günlük gövdelerin en yüksek E vitamini içeriğinesahip olduğu bulundu. C vitamini içeriğine göre, 7 günlük gövdelerin 10 ve 14 günlük gövdelere göre daha yüksek olduğu belirlendi. E vitamini sentezinde görevli diğer bazı genlerin çalışılması, bu genlerin mutantları veya gen susturma teknolojileri ile fonksiyonu değiştirilmiş genlerin çalışılması ileri çalışmalar olarak düşünülebilir.
Investigation of the Protective Effects of Horse Mushroom (Agaricus Arvensis Schaeff.) Against Carbon Tetrachloride-Induced Oxidative Stress in Rats
(Springer, 2018) Dogan, Abdulahad; Dalar, Abdullah; Sadullahoglu, Cemil; Battal, Abdulhamit; Uzun, Yusuf; Celik, Ismail; Demirel, Kenan
Wild and cultured mushrooms have been extensively used for food and medicinal purposes all around the world. However, there is limited information on chemical composition, health enhancing effects and contributions on diet of some mushrooms (e.g., Agaricus arvensis) widely distributed in many countries including United Kingdom, Australia, Turkey etc. Therefore, the present study was aimed to analyse the bioactive composition and ameliorative effects of A. arvensis via evaluating in vitro and in vivo antioxidant properties in CCl4 induced rat model. The extract exhibited higher antioxidant capacities in vitro than that of the positive control (Reishi-Shiitake-Maitake standardized extract). Administration of the extract had significant regulative effects in the levels of AST, ALT, LDH, Urea and TRIG levels according to CCl4 group. Additionally, lipid peroxidation and GSH in the brain, kidney and liver tissues was regulated by extract treated groups compared to the CCI4 group. The supplementation of the extract at the dose of 100 mg/kg regulated the levels of GST, GR, CAT and GPx enzyme activities in brain and liver, but not in kidney tissue. There was approximately three fold increase in CAT enzyme activity in kidney tissue of extract treated groups compared to Control and CCl4 groups. The extract contained a rich composition of bioactive compounds including phenolics (protocatechuic acid and p-hydroxybenzoic acid), volatile compounds (benzaldehyde, palmitic acid and linoleic acid) and mineral compounds (K, Si, Mg and Na). Data obtained within this study suggests that A. arvensis might be used for food industries in order to obtain nutritional products.
Synthesis and Antibacterial Activity of 4-Benzoyl Acid and Derivatives
(Springer Birkhauser, 2009) Bildirici, Ishak; Sener, Ahmet; Atalan, Ekrem; Battal, Abdulhamit; Genc, Hasan
A new 1H-pyrazole-3-carboxylic acid 2, along with hydrazono-pyridazinone 3, a by-product, and its derivatives 4-7 were synthesized and the structures confirmed by infrared (IR) and H-1 and C-13 nuclear magnetic resonance (NMR) data. These new compounds were evaluated for their antibacterial activities against Gram-positive and Gram-negative bacteria using the tube dilution method. The minimal inhibitory concentrations (MICs) experiments revealed that most compounds exerted inhibitor effects against Klebsiella pneumonia, Escherichia coli, Bacillus subtilus, and Xanthomonas compestris test microorganisms. Moreover, the results showed that the pyrazolo[3,4-d]pyridazine compounds were the best compounds of the series, exhibiting antibacterial activity against both Gram-positive and Gram-negative bacteria.
Synthesis of Silver Nanoparticles From Summer Snowflake Plant (leucojum Aestivum L.) and Determination of Biological Activities
(2024) Türkmen, Nusret; Battal, Abdulhamit
Türkmen N, Göl Soğanı Bitkisinden (Leucojum aestivum L.) GümüĢ Nanoparçacıkların Sentezlenmesi ve Biyolojik Aktivitelerinin Belirlenmesi, Van Yüzüncü Yıl Üniversitesi. Sağlık Bilimleri Enstitüsü, Temel Eczacılık Bilimleri Anabilim Dalı, Yüksek Lisans Tezi, Van, 2024. Bu çalıĢmanın amacı, Leucojum aestivum L. (göl soğanı) bitkisinden yeĢil sentez yöntemiyle gümüĢ nanopartiküllerin (La-AgNP) elde edilmesi, karakterizasyonu ve biyolojik aktivitelerinin incelenmesidir. ÇalıĢmada, L. aestivum bulblarından elde edilen infüzyon kullanılarak La-AgNP'ler sentezlenmiĢtir. Sentez parametreleri (sıcaklık, gümüĢ nitrat konsantrasyonu, ekstre konsantrasyonu, pH ve süre) optimize edilmiĢtir. Elde edilen nanopartiküller UV-Vis spektrofotometresi, FTIR, SEM ve Zeta Sizer analizleri ile karakterize edilmiĢtir. Bitkinin fenolik içeriği HPLC ile analiz edilmiĢtir. La-AgNP'lerin antimikrobiyal aktivitesi, gram-pozitif ve gram-negatif bakterilere karĢı minimum inhibisyon konsantrasyonu (MĠK) ve bakterisidal etki testleriyle değerlendirilmiĢtir. Sitotoksisite ve antikanser etkileri MRC-5 ve CaCo-2 hücre hatları üzerinde incelenmiĢtir. Sonuçlar, La-AgNP'lerin optimum sentez koĢullarının 2 mM AgNO₃, %15 ekstre konsantrasyonu, 40°C reaksiyon sıcaklığı, pH 9 ve 10 dakika reaksiyon süresi olduğunu göstermiĢtir. Karakterizasyon çalıĢmaları, nanopartiküllerin küresel morfolojiye sahip olduğunu ve ortalama boyutunun 74.7 nm olduğunu ortaya koymuĢtur. HPLC analizleri, ekstrede gallik asit, kateĢin, askorbik asit ve kersetin gibi fenolik bileĢiklerin varlığını göstermiĢtir. La-AgNP'ler, test edilen tüm bakteri türlerine karĢı değiĢen derecelerde antimikrobiyal aktivite sergilemiĢtir. Sitotoksisite testlerinde, La-AgNP'lerin test edilen konsantrasyon aralığında (1.5-12 μg/mL) normal hücreler üzerinde minimal etki gösterdiği, ancak test edilen konsantrasyonlarda belirgin bir antikanser etki göstermediği belirlenmiĢtir. Bu çalıĢma, L. aestivum bitkisinden elde edilen gümüĢ nanopartiküllerin potansiyel antimikrobiyal ajan olarak kullanılabileceğini göstermiĢ, ancak antikanser uygulamalar için daha ileri araĢtırmaların gerektiğini ortaya koymuĢtur.
Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması
(2019) Görmez, Gül; Turker, Musa; Battal, Abdulhamit
Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazı türleri içermektedir. Alcea kurdicaAlef. (Deve gülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemi enfeksiyonlarına ve saç kırana karşıkullanılan tıbbi bir bitkidir. Bu çalışmanın amacı, deve gülü hatmi bitkisi için steril şartlar altında etkili birçimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürü çalışmalarında kullanılabilir hale getirilmesidir.Bu amaç için, steril bir ajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°C sıcaklıktainkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesi uygulamaları test edilmiştir. %5 ve %7.5 sodyumhipoklorit konsantrasyonlarının sterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipokloritkonsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme oranları sonuçlarına göre, kabuğu çentiklenentohumlar hızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir. Yedinci günde,kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürü için kullanılabilir duruma gelmiştir. Sekizinci günde,yapraklar oluşmaya başlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu günde dokukültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma ile tıbbi öneme sahip deve gülü hatmi içinetkili bir çimlendirme yöntemi geliştirilmiştir.
Xanthoparmelia Somloensis (Gyelnik) Hale Likeninin Liyofilize Etanol Ekstraktının Domates Bitkisi Üzerindeki Allelopatik Etkilerinin Araştırılması
(2022) Aslan, Ali; Bingöl, Ömer; Erez, Mehmet Emre; Battal, Abdulhamit
Doğadaki tüm organizmalar birbirleriyle etkileşim ve rekabet halindedir. Algler, likenler, kültür bitkileri ve yabani otlar gibi çeşitli organizma gruplarının allelopatik etkileşime sahip oldukları ve likenlerin birçok güçlü sekonder metabolite sahip olduğu bilinmektedir. Bu çalışmanın amacı, Xanthoparmelia somloensis (Gyelnik) Hale liken (XS) liyofilize etanolik ekstraktının domates (Lycopersicum esculentum L.) çimlenmesi ve erken gelişme aşaması üzerindeki allelopatik etkilerinin belirlenmesidir. 50 ppm (XS-50), 100 ppm (XS-100), 200 ppm (XS-200) ve 400 ppm (XS-400) konsantrasyonlarında XS likeninin elde edilen liyofilize etanolik ekstreleri domates tohumlarına ve fidelerine uygulandı. Ekstraktların neden olduğu etkileri belirlemek için, çimlenme oranı ve fide büyüme parametreleri (canlılık indeksi, uzunluk, taze ağırlık, bağıl su içeriği ve pigment içeriği) analiz edildi. Elde edilen verilere göre liken ekstraktı konsantrasyonundaki artışa bağlı olarak çimlenme hızı ve vigor indeks değerlerinde önemli düşüşler tespit edildi. Benzer etkiler kök ve sürgün uzunluğu ve pigment içeriklerinde de gözlendi. Ancak en düşük uygulamanın (XS-50) sonuçları kök uzunluğu ve karotenoid içeriği dışında kontrole benzerlik gösterdi. Bu çalışmadan elde edilen veriler, Xanthoparmelia somloensis'in allelopatik etkilere neden olduğu ve tarımsal amaçlı kullanım potansiyeline sahip olduğunu göstermiştir.