Browsing by Author "Baydas, B"

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Early Changes in Glial Reactivity and Lipid Peroxidation in Diabetic Rat Retina: Effects of Melatonin
(Springer-verlag Italia Srl, 2004) Baydas, G; Tuzcu, M; Yasar, A; Baydas, B
Diabetes mellitus, the most common serious metabolic disorder, is characterized by functional and structural changes in the peripheral and central nervous systems. Glial cells provide structural and metabolic support for retinal neurons. During diabetes, one of the early pathogenic events is retinal glial reactivity. We studied the effects of melatonin, which is known to reduce oxidation-based neurotoxicity, on glial reactivity and lipid peroxidation in the retina of diabetic rats. Diabetes was induced by a single injection of streptozotocin (STZ), and these diabetic rats were treated daily either with melatonin (10 mg/kg) or saline vehicle. After 6 weeks of diabetes, we determined the extents of lipid peroxidation and glial C, reactivity in retina. Lipid peroxidation, measured on the basis of malondialdehyde and 4-hydroxyalkenals concentrations, was increased in diabetic rats (p<0.01) and this increase was prevented by melatonin treatment (p<0.05). Furthermore, gial reactivity, determined immunohistochemically from the levels of glial fibrillary acid protein (GFAP), was also increased significantly (p<0.01). Melatonin administration partially prevented this increase in GFAP content (p<0.05). In conclusion, glial reactivity is an early pathogenic event in diabetic retina and both reactive gliosis and accumulation of malondialdehyde and 4-hydroxyalkenals are prevented by melatonin supplementation.
Effect of Nigella Sativa L. on Heart Rate and Some Haematological Values of Alloxan-Induced Diabetic Rabbits
(Scandinavian Federation Laboratory Animal Science, 2004) Meral, I; Donmez, N; Baydas, B; Belge, F; Kanter, M
This study was designed to investigate the effect of an extract of Nigella sativa L. on the heart rate and some haematological values in alloxan-induced diabetic rabbits. Fifteen New Zealand male rabbits were divided into three experimental groups: control, diabetic and N. sativa L.-treated diabetic. At the end of the experimental period (2 months), animals in all three groups were fasted for 12 hours and blood samples were taken for the determination of glucose levels, RBC and WBC (red and white blood cell) counts, packed cell volume (PCV), and haemoglobin (Hb) concentration. Heart rates were also measured by a direct-writing electrocardiograph before the blood withdrawals. It was found that N. sativa L. treatment increased the lowered RBC and WBC counts, PCV and neutrophil percentage in diabetic rabbits. However, the WBC count of the X sativa L. treated diabetic group was still lower than the control. N. sativa L. treatment also decreased the elevated heart rate and glucose concentration of diabetic rabbits. It is concluded that oral X sativa L. treatment might decrease the diabetes-induced disturbances of heart rate and some haematological parameters of alloxan-induced diabetic rabbits.
Effects of Biological Extracellular Matrix Components on in Vitro Axonal Regeneration
(Amer Assoc Clinical Chemistry, 2005) Sekeroglu, MR; Ozturk, G; Baydas, B
Effects of Melatonin on Lipid Peroxidation and Anti-Oxidant Enzyme Activity in Rats With Experimentally Induced Hyperthyroidism
(Wiley, 2005) Baydas, B; Meral, I
1. The present study was designed to investigate the effects of high-dose melatonin on lipid peroxidation and anti-oxidant enzyme activity in rats with experimentally induced hyperthyroidism. 2. Twenty-four albino male rats, weighing 240-260 g, were randomly allotted into one of three experimental groups (control, hyperthyroid and hyperthyroid + melatonin treatment), with each group containing eight animals. Hyperthyroidism was induced by a daily with i.p. injection of 200 mu g L-thyroxine for 30 days. In addition to L-thyroxin treatment, rats in the hyperthyroid + melatonin treatment group were also given daily i.p. injections of 10 mg/kg melatonin on the last 10 days of L-thyroxine treatment. Control animals received injections of an equivalent volume of saline solution. Rats received the last injection 24 h before being killed. 3. At the end of the experiment, rats in all three groups were fasted for 12 h and killed by cardiac puncture under ether anaesthesia. Blood samples were taken for the determination of malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) levels and concentrations of tri-iodothyronine (T-3) and thyroxine (T-4). 4. It was found that MDA and SOD levels and concentrations of T-3 and T-4 were higher and the GSH level was lower in rats with hyperthyroidism compared with controls. Melatonin treatment decreased the elevated MDA and SOD levels and increased the lowered GSH level to control levels in rats with hyperthyroidism, but did not ameliorate the concentrations of T-3 and T-4. 5. It was concluded that high-dose melatonin treatment may decrease the hyperthyroidism-induced disturbances of lipid peroxidation and anti-oxidant enzyme activity and oxidative damage.
Effects of Oral Zinc and Magnesium Supplementation on Serum Thyroid Hormone and Lipid Levels in Experimentally Induced Diabetic Rats
(Humana Press inc, 2002) Baydas, B; Karagoz, S; Meral, I
This study was designed to investigate the effects of oral zinc and magnesium supplementation on serum thyroid hormone and lipid levels in alloxan-induced diabetic rats. Thirty-two albino male rats, weighing 234 34 g, were divided into four experimental groups (control, diabetic, diabetic+zinc supplemented and diabetic+ magnesium supplemented). The experiment lasted for 60 d. The first 45 d of the experiment was the supplementation and last 15 d was the supplementation and diabetes-inducing period. Diabetic+zinc-supplemented and diabetic+magnesium-supplemented groups were given orally (by adding in their drinking water) 227 mg/L of zinc and 100 mg/kg body weight (bw) of magnesium, respectively throughout the experiment. Control and diabetic groups served as controls and did not receive zinc or magnesium supplementation. Diabetic, diabetic+zinc-supplemented, and diabetic+magnesium-supplemented groups were given a daily injection (ip) of 100 mg/kg bw of alloxan for 15 d starting on d 46 of the experiment. The control group was only injected with the same volume of isotonic NaCl as the diabetic group received. At the end of the of the experiment, rats in all four groups were fasted for 12 h and blood samples were taken from the heart under ether anesthesia for the determination of thyroid hormone, glucose, total cholesterol, and triglyceride concentrations. It was found that serum glucose, total cholesterol, and triglyceride concentrations were higher and serum T3 and T4 concentrations were lower in diabetic rats than those in the control group. Zinc supplementation did not change any parameter in diabetic rats. However, magnesium supplementation decreased the elevated total cholesterol and triglyceride concentrations of the diabetic rats to the control level. It was concluded that oral magnesium supplementation might decrease the diabetes-induced disturbances of lipid metabolism.
Role of Vitamin E in Decreasing the Toxic Effect of Digoxin
(Scientific Technical Research Council Turkey, 1999) Meral, I; Baydas, B; Bayiroglu, F; Uysal, H
This experiment was carried out to evaluate the role of Vitamin E in decreasing the toxic effect of digoxin. Electrocardiographic recordings of New Zealand rabbits which were treated with digoxin-only or digoxin+vitamin E were used. Recordings were obtained before and 24 hours after treatments. It was found that digoxin-only treatment caused a sinusoidal tachicardia without changing the durations or amplitudes of any wave on the trace. However, digoxin+vitamin E treatment did not produce any increase in heart rate. It was concluded that Vitamin E injection along with digoxin injection might lower the toxic effect of digoxin. More studies are needed to convince these findings.
Studies on Some Haematological and Biochemical Parameters in Clinically Healthy Ostriches
(Tubitak Scientific & Technological Research Council Turkey, 2003) Agaoglu, Z; Yoksek, N; Altug, N; Kaya, A; Baydas, B
This study was carried out to determine the normal haematological and biochemical parameters of clinically healthy ostriches 1-3 years old of either sex. Red blood cells, white blood cells, haematocrit and haemoglobin values (as haematological parameters) and glucose, total protein, albumin, globulin, urea, kreatinine, serum enzymes (ALP, AST, ALT, GGT, CK, amilase) (as biochemical parameters) and electrolytes (Ca, P, Mg, Zn, Cu, Na, K, Cl) levels were measured. It was concluded that the values obtained in this study could be used as reference values for ostriches.