Browsing by Author "Bektas, Abdullah"

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Comparison of Bacterial Culture and Microscopic Urine Analyzer in the Urinary Tract Infections
(Aves, 2013) Guducuoglu, Huseyin; Bektas, Abdullah; Gultepe, Bilge; Balahoroglu, Ragip; Bayram, Yasemin
Objective: The urine culture is the gold standard for diagnosis of urinary tract infection. Fully automated urine analyzer (FAUA) which gives quantitative results is also commonly used for urinalysis. This device identifies erythrocytes, leukocytes and epithelial cells as well as bacteria and renal elements performing a microscopic analysis. The aim of this retrospective study is to compare urine cultures with the FAUA used by the clinicians for rapid diagnosis of urinary tract infections. Methods: A total number of 23 065 urine specimens sent from various clinics to the Microbiology and Clinical Microbiology Laboratory in our hospital for both urinalysis and urinary culture between the dates of 1 June 2010 and 31 October 2011 were evaluated. Urinalysis was performed with iQ (R) 200 (Iris Diagnostics, Chatsworth, CA, USA). The FAUA values with >= 5 leukocytes and >= 1 bacteria were classified as FAUA-positive. Urine cultures growing >= 5x10(4) cfu/ml were included in the comparison. Results: Of the total FAUA and culture results, 11.7% were incompatible in terms of the number of leukocytes including leukocytenegative/culture-positive (0.4%) and leukocyte-positive/culturenegative (11.3%) results, and 6.6% were incompatible in terms of the number of germs including culture-positive/germ-negative (5%) results and germ-positive/culture-negative (1.6%) results. Conclusions: FAUA often used by clinicians for rapid diagnosis of urinary tract infection will contribute to the evaluation of the culture in a more efficient way, however, it should not replace the urine culture.
Evaluation of Antibiotic Resistance in Acinetobacter Baumannii Strains Isolated From Various Clinical Samples
(Doc design informatics Co Ltd, 2013) Bayram, Yasemin; Gultepe, Bilge; Bektas, Abdullah; Parlak, Mehmet; Guducuoglu, Huseyin
Objective: Treatment of Acinetobacter baumanii infections has become increasingly difficult, due to the high resistance rates against commonly administered antibiotics. This study aims to determine the antibiotic resistance of A. baumannii strains isolated in our hospital. Methods: Antibiotic resistances of 377 A. baumannii strains isolated from various clinical specimens between 2007-2011 were assessed retrospectively. The isolates were identified by conventional methods and Phoenix 100 (Becton Dickinson Co., Sparks, MD, USA) automated system. Antibiotic susceptibility tests were performed according to the standards of Clinical and Laboratory Standards Institute. Results: Colistin resistance was not observed, and resistances to the other antibiotics were found to be 64% to amikacin, 55% to tetracycline, 71% to imipenem, 72% to meropenem, 89% to cefoperazone-sulbactam and 92% to ciprofloxacin, 90% to gentamicin, 94% to ceftazidime, 96% to piperacillin-tazobactam, 95% to cefepime and 96% to cefotaxime. Conclusions: Knowledge of susceptibilitys rates of these bacteria is essential, especially in situations compelling empirical treatment.
Investigation of Extended Spectrum Beta-Lactamase (Esbl) Genes in Esbl-Producing Escherichia Coli and Klebsiella Pneumoniae Strains
(Bilimsel Tip Yayinevi, 2018) Bektas, Abdullah; Guducuoglu, Huseyin; Gursoy, Nafia Canan; Berktas, Mustafa; Gultepe, Bilge Sumbul; Parlak, Mehmet; Takerekoglu, Mehmet Sait
Introduction: Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is an important health problem all over the world. In this study, it was aimed to determine the ESBL genes in Escherichia coli and Klebsiella pneumoniae strains isolated for approximately four-year period. Materials and Methods: A total 100 ESBL-producing E. coli and 100 ESBL-producing K. pneumoniae strains which were isolated between January 2008 and October 2012 were included into this study. The strains were identified using classical bacteriologic methods and BD Phoenix (Becton Dickinson, US) automatized bacterial identification device. CTX-M, TEM, SHV, VEB, GES, PER and OXA beta-lactamase genes were analyzed with the PCR method. Results: The beta-lactamase genes detected in ESBL-positive K. pneumoniae strains were as follows: 99% for CTX-M, 91% for SHV, 71% for TEM, 10% for OXA-10 group, and 5% for OXA-2 group. In E. coli strains, the prevalence of CTX-M was 92%; TEM was 70%, SHV was 21%, and OXA-2 group was 3%. CTX-M alone was found to be positive in 25 of the 98 (25.5%) in E. coli strains; TEM alone was found to be positive in 2 of 98 (2%) and SHV alone was found in 2 of 98 (2%). CTX-M alone was found positive in 3 of 100 (3%) K. pneumoniae strains. No other resistance genes alone were found in the strains. No GES, VEB and PER-producing strains were determined in this study. Conclusion: In the study, high prevalence of CTX-M beta-lactamase was found in ESBL-producing strains. It was thought that the high potential of mobility with CTX-M genes was the most possible reason for this result. Determination of ESBL genes will be useful to understand resistance epidemiology, develop effective therapeutic strategies, and plan the appropriate preventive measurements.
Sepsis Caused by Chryseobacterium Indologenes in a Patient With Hydrocephalus
(Ankara Microbiology Soc, 2011) Ceylan, Abdullah; Guducuoglu, Huseyin; Akbayram, Sinan; Bektas, Abdullah; Berktas, Mustafa
Chryseobacterium (formerly Flavobacterium) indologenes, is a non-fermentative gram-negative bacillus which is widely found in the nature, primarily soil and water. Since it can survive in chlorine-treated municipal water supplies, and can colonize the sink basins and tap waters of the hospitals, this bacterium may be a potential infectious agent. Contamination of the medical devices containing water (respirators, intubation tubes, humidifiers, incubators for newborns, etc.) in hospital settings may lead to serious infections especially in patients with predisposing diseases, newborns and immunocompromized patients. In this report, a case of fatal C.indologenes septicemia developed in a newborn with hydrocephalus has been presented. A two-months old male infant was admitted to our hospital with the complaints of failure to suck and lethargy for five days and head enlargement. He was diagnosed as meningitis based on the clinical and laboratory findings of cerebrospinal fluid (CSF) (protein: 572 mg/dl, glucose 9.5 mg/dl, chlorine: 111 mg/dl, and presence of abundant polymorphonuclear leukocytes), and empirical antibiotic treatment (ampicillin/sulbactam and cefotaxime) had been started. Since the computerized tomography of the brain pointed out hydrocephalus, an external shunt was placed for CSF drainage on the second day of hospitalization. A total of five CSF and two blood cultures collected during the hospitalization period were inoculated into pediatric aerobic CSF and blood culture bottles (BacT/ALERT, BioMerieux, France) and incubated for 24-48 hours. The isolated bacteria from all of the cultures were identified as C.indologenes by conventional methods and BD Phoenix (Becton Dickinson, USA) system. Antibiotic susceptibility tests were performed with microdilution method according to CLSI guidelines. The isolate was found susceptible to ciprofloxacin, levofloxacin and trimethoprim/sulfamethoxazole, while it was resistant to amikacin, gentamicin, tobramycin, piperacillin, cefotaxime, ceftazidime, aztreonarn, meropenem, imipenem, tetracycline, and chloramphenicol. The treatment continued with ampicillin/sulbactam and levofloxacin without removing the shunt. However, C.indologenes growth persisted in CSF and blood cultures of the patient. The general condition of the patient deteriorated on the 65. day of the hospitalization and the patient was lost due to cardiopulmonary arrest. Case reports related to isolation of C.indologenes from blood cultures are present in the literature, however, isolation of C.indologenes from central nervous system was reported previously in a single case. In conclusion, C.indologenes should be considered as opportunistic infectious agents especially in the infectious diseases that develop in immunocompromised patients with underlying disease and with foreign device implementation.