Browsing by Author "Belhan, S."

Now showing 1 - 7 of 7

Changes in Sperm Parameters Following Administration of Theophylline, a Competitive Antagonist of Adenosine in Rats Exposed To Bleomycin, a Chemotherapeutic Agent
(Ataturk Universitesi, 2019) Belhan, S.; Oto, G.; Arihan, O.; Koşal, V.
The aim of the present study is to determine the effects of theophylline (THP), a methylxanthine derivative, on the sperm parameters of rats exposed to bleomycin (BLM) as a chemotherapeutic agent. The study was performed on 32 male rats. The rats in Group 1 were administered only with sterile saline intratracheally. The rats in Group 2 were administered a single dose of 7.5 mg/kg of BLM intratracheally. The rats in Group 3 received a single dose of 7.5 mg/kg of BLM intratracheally. In addition, THP was administered to rats in Group 3 intraperitoneally at a dose of 75 mg/kg/ day for 14 days. The rats in group 4 were administered an intraperitoneal injection of THP at a dose of 75 mg/kg/day for 14 days. Motility, sperm density, abnormal sperm and dead sperm rates were examined. Significant deteriorations were observed in sperm parameters of rats that were administered BLM. In sperm parameters of rats that were administered THP, it was determined that it only increased motility, but did not significantly change sperm density, abnormal sperm and dead sperm count. In this study, THP only increased motility. There was no positive effect on sperm parameters except motility. © 2019 Ataturk Universitesi. All rights reserved.
Collection of Semen From Van Cats Using Electroejaculation and Freezing of Semen
(Mehria Publishers, 2018) Belhan, S.; Gülyüz, F.
In this study, the aim was to collect semen from Van Cats using electroejaculation, to perform spermatologic examinations and to freeze the semen. In the research, 7 Male Van Cats were used. Semen was collected from cats at 10 different times at weekly intervals using electroejaculation with general anesthesia. The semen was diluted with 3 diluents (3% Equiex, 6% Equiex, Biosfos). Motility and vitality values were analyzed. Then the semen was frozen in liquid nitrogen vapor. The semen dissolved was analyzed for motility and vitality values. In the study, amount of semen, sperm motility, dead sperm, sperm concentration, total abnormal sperm, stimulation time and stimulation count were found to be 91.92±21.49 μl, %67.42±4.94, %23.91±8.59, 109.20±66.12x106/ml, %25.57±4.57, 83.08±4.57 sec and 10.35±0.56, respectively. After dissolution, motility and vitality values were found to be, respectively, 45.79±5.49% and 53.57±6.03% with 3% Equiex diluent, 41.78±5.71% and 52.64±6.35% with 6% Equiex diluent, 40.00±5.45% and 51.50±6.72% with Biosfos diluent. After the dissolution, abnormal sperm rates related to the head, middle piece and tail were 3.74±0.91%, 13.62±2.63%, 21.91±4.03%, respectively. In conclusion, approximately 43% motility achieved with the freezing and dissolution of the semen suggests that the semen of Van Cat can be frozen with 43% success. © 2018 IJVS. All rights reserved.
Effect of Gnrh Administration at Different Times After Synchronisation on Pregnancy Rates in Cows
(Wiley-blackwell, 2014) Uslu, B. A.; Belhan, S.; Gulyuz, F.; Sendag, S.; Wehrend, A.
Effects of Chronically Exposure To Flor and 7,12-Dimethylbenzanthracene (Dmba) on Spermatogenesis and Testicular Histopathology in Rats
(Ataturk Universitesi, 2018) Yildirim, S.; Belhan, S.; Uyar, H.; Huyut, Z.; Oto, G.; Sağlam, Y.S.
This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH) derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testis histopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment, intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosterone levels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratio of motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in rats significantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubulus wall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg/kg) group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes in the tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg/kg) were examined, atrophy in seminiferous tubules, severe degenerative and necrotic changes in Spermatogoniums on the walls of tubules and thinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in the intertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF and DMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated. © 2018 Ataturk Universitesi.All Rights Reserved.
The Effects of Curcumin Nanoparticles on an Experimental Diabetes Model: Reproductive Hormones, Lipid Profile and Immunohistochemical and Immunofluorescence Markers
(B W K Publishing Solutions & verlag, 2024) Belhan, S.; Huyut, Z.; Yildirim, S.; Algul, S.
Diabetes affects many organs and causes serious problems for reproductive health. There is still no effective treatment but a range of new strategies are being used to treat and to reduce the possible complications of diabetes. One of the most promising of them relies on the use of nanoparticles. We have evaluated the effects of curcumin and curcumin nanoparticles on blood glucose, lipid profile, reproductive hormones and histopathological, immunohistochemical and immunofluorescent parameters in experimental diabetic conditions. We created eight study groups (control group, diabetes group, curcumin group, Mg-curcumin nanoparticle group, diabetes+curcumin group, diabetes+Mg-curcumin nano- particle group, encapsulated Mg-curcumin nanoparticle group, diabetes+encapsulated Mg-curcumin nano- particle group), allocating rats to the groups at random. Triglyceride, cholesterol and LDL levels were high in the diabetes group, NOP10 and PI3K levels were intense and reproductive hormone levels were low. Curcumin and curcumin nanoformulations administered to the diabetes groups led to significant improvements in these parameters. The testosterone level, which was low in the diabetes group, increased significantly after the application of encapsulated Mg-curcumin. Curcumin nanoformulations were more effective than curcumin at countering the diabetes-impaired lipid profile and improving the levels of reproductive hormones, the histopathological findings and PI3K and NOP10 expression.
Endocrine and Cytological Responses To Oral Administration of Altrenogest at the Onset of the Breeding Season in Coloured Mohair Goats
(Wiley-blackwell, 2014) Uslu, S.; Belhan, S.; Uslu, B. A.; Cetin, N.; Yoruk, M.; Gulyuz, F.; Wehrend, A.
Induction of Ovulation With Gonadotropins in Turkish Van Cats and Investigation of the Effect of the Number of Coitus Events on Fertility
(Wiley-blackwell, 2014) Uslu, B. A.; Belhan, S.; Gulyuz, F.; Karaca, M.; Sendag, S.; Wehrend, A.