Browsing by Author "Bengu, A. S."

Now showing 1 - 3 of 3

Ameliorative Effect of Astaxanthin on Ischemia-Reperfusion Injury of Skeletal Muscles
(Pakistan Agricultural Scientists Forum, 2022) Uyar, A.; Akkoyun, H. T.; Bengu, A. S.; Akkoyun, M. B.; Keles, O. f.; Atcali, T.; Yaman, T.
This experimental study aimed to investigate the ameliorative effect of astaxanthin (AST) on the prevention of skeletal muscle injury resulting from lower extremity ischemia/reperfusion (I/R). Twenty-eight (250-300g) male Wistar albino rats were divided into 4 groups as Control, I/R, I/R+AST and AST. In the control group, only anesthesia was induced for 2 h without I/R. In the I/R group, 2 h of reperfusion was facilitated following ischemia under anesthesia. For the I/R+AST group, 7 days prior to ischemia, 125 mg/kg AST was given through a gavage, and 2 h of ischemia and 2 h of reperfusion were facilitated under anesthesia. At the end of the study, blood and gastrocnemius muscle tissue samples were taken for biochemical, histopathological and immunohistochemical examinations. Compared to the control group, there were increased Malondialdehyde (MDA) levels and decreased Superoxide dismutase (SOD) and Catalase (CAT) enzyme activities in the I/R group (p??0.001). Degeneration, necrosis, inflammation, loss of striation, interfibrillar and interfascicular edema were seen in the histopathological examination of the skeletal muscles in the I/R group. These histopathological findings were minimal in the I/R+AST group. In the immunohistochemical examination of muscle tissue with the GPx1 primary antibody, a mild degree of GPx1 reactivity was observed in the I/R group, and a moderate degree of GPx1 reactivity was seen in the I/R+AST group. As a result, the strong ameliorative effect of AST on ischemia-reperfusion injury and its complications on skeletal muscles was demonstrated by biochemical, histopathological and immunohistochemical examinations.
Gundelia Rosea Seed: Evaluation of Biopharmaceutical Potential and Bioactive Composition
(Elsevier, 2019) Dalar, A.; Zengin, G.; Mukemre, M.; Bengu, A. S.; Isler, S.
Gundelia species are among significant key medicinal plants extensively utilized in folk medicine of Middle Eastern countries. This study focused on researching the biopharmaceutical potency and bioactive compounds of Gundelia rosea seed. Hereby, traditional knowledge-based preparing methods (infusion and decoction) and ethanol-based lyophilized extracts obtained from Gundelia rosea seeds were assessed for (i) antioxidant capacities, (ii) enzyme inhibitory activities, (iii) HPLC-MS/MS and (iv) GC-MS studies. Phytochemical analysis revealed that ethanol extract which primarily compromised of mainly phenolics (4-Caffeoylquinic acid and luteolin hexoside) and several fatty acids (palmitic, stearic, oleic and linoleic acids), was superior to those of infusion and decoction extracts. Antioxidant activities findings revealed that ethanol extract contained a high level of total phenolics (55.3 mg Gallic acid Eq./g extract) and had high capacities of reducing (1683 mu mol Fe2+ and 214.1 mg Trolox Eq./g extract for FRAP and CUPRAC respectively) and radical scavenging (ORAC: 2241.9 mu mol, DPPH: 91.7 mg, ABTS: 141.2 mg Trolox Eq./g extract) and total antioxidant (Phosphomolybdenum: 1.39 mmol Trolox Eq./g extract) properties. The suppressive abilities of the extracts against selected isolated enzymes revealed that ethanol extract had pronounced levels of inhibitory activities against AChE (4.3 mg Galanthamine Eq.), BChE (3.4 mg Galanthamine Eq.), tyrosinase (120 mg Kojic acid Eq.), amylase (0.61 mmol Acarbose Eq.), glucosidase (11.91 mmol Acarbose Eq.) and lipase (53.4 mu mol Orlistat Eq.) per gram extract. Findings obtained within this study confirmed the traditional utilization of Gundelia rosea and suggest its potential as a novel candidate of biopharmaceutical agents for public health problems. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.
Protective Effect of Astaxanthin in the Lung Injury Caused by Ischemia Reperfusion of the Lower Extremities
(Pakistan Agricultural Scientists Forum, 2019) Akkoyun, H. T.; Uyar, A.; Bengu, A. S.; Akkoyun, M. Bayramoglu; Arihan, O.; Keles, O. F.
Pathological and biochemical alterations due to lower extremity (I/R) damage and protective effects of astaxanthine (AST) were investigated. Rats were divided into four groups. GI-Sham group (n=7):Anesthesia without (I/R)(2hours);GII-I/R (n=7) : 2 hours of ischemia and 2 hours of reperfusion under anesthesia; Group III-AST(n=7): Rats were subchronically orally administered for 7 days at 125 mg/kg astaxanthin (AST) and then anesthetized (2hours) without ischemia; GIV-I/R+AST (n=7) : 7 days prior to ischemia rats were subchronically orally administered 125 mg/kg astaxanthin (AST) and then 2 hours of ischemia and reperfusion under anesthesia; Then lung tissues were investigated for MDA,GSH and histopathology. An increase in MDA and a decrease in GSH was observed I/R administered group compared to control. Histopathological evaluations showed intense congestion in pulmonary veins and alveolar septum and partial alveolar macrophage and erythrocyte accumulation and edema was observed in lumens of some bronchioles and alveoli in the second and fourth group compared control. Second group (3.41) damage score had high significance compared to control (p <= 0.001). Fourth group damage score (0.92) was indifferent from control but significantly different from I/R group (p <= 0.001). As a result; The protective effect of AST has been demonstrated by biochemical, histopathological and immunohistochemical effects.