Browsing by Author "Berber, I"

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Antibacterial and Antifungal Activities of New Pyrazolo[3,4-D]pyridazin Derivatives
(Elsevier France-editions Scientifiques Medicales Elsevier, 2005) Akbas, E; Berber, I
Several new pyrazolo[3,4-d]pyridazin derivatives were prepared by the reaction of two new 1H-pyrazole-3-carboxylic acids and various hydrazines. The compounds were tested for antimicrobial activities against Gram-negative, Gram-positive bacteria and fungi. The compounds named as 7e, f had the highest antimicrobial activities against Gram-negative, Gram-positive bacteria and fungi with minimum inhibitory concentrations in the range of 0.31 to < 0.0024 mg ml(-1). (c) 2005 Elsevier SAS. All rights reserved.
Characterization of Staphylococcus Species by Sds-Page of Whole-Cell and Extracellular Proteins
(Maik Nauka/interperiodica, 2003) Berber, I; Cokmus, C; Atalan, E
In this study, a total of fifteen staphylococcal strains belonging to different species were characterized by whole-cell and extracellular protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results are presented as dendrograms after quantitative analysis of the band patterns with a computer program. Visual inspection of protein bands and cluster analysis of protein patterns of to be used 15 strains, representing 10 Staphylococcus species, showed that whole-cell and extracellular protein profiles differed in several protein bands in Staphylacoccus aureus, S. epidermidis, S. simulans, and other species of Staphylococcus; however, the differences were insufficient for reliable differentiation of Staphylococcus species by the SDS-PAGE method.
Determination of Total Cell Protein Profiles of Streptomyces Species
(Journal Experimental Biology & Agricultural Sciences, 2013) Zdemir, K.; Berber, I; Ogun, E.; Atalan, M.
Present study has been conducted for finding out the total protein profile of bacterial strain Streptomyces sps by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Total 139 isolates of Streptomyces have been isolated from the soil. Amongst all isolated strain, total 20 isolates were used for getting protein profile by SDS PAGE. Amongst all isolates, 20 isolates were selected for protein profiling and these were divided in two groups. Two strains of Streptomyces i.e. S. violaceus and S. albidoflavus were selected as a reference strain for both groups. Band profile were analyzed and assessed by computer added program BioRad Quantity with the use of Unweighted Pair Group Method of Analysis (UPGMA). As a result of this computer assisted numeric analysis study, approximately 40 different types of protein bands were reported between 10 or 100 kD molecular weight. Analysis of acquired dendogram on the basis of similarities ratios, all 40 proteins can be divided in to 7 groups. In addition, the isolates A4B3G, D145B, S5036.6 and reference isolate S. violaceus were available in the same group, while 805A, C804B, F1705 isolates and reference sample S. albidoflavus were detected in the same group. The test organisms which were similar to each other in terms of morphological and biochemical characters delivered the same protein bands. SDS-PAGE method is an effective method in terms of determining taxonomical relations between the various species of genus Streptomyces.
Effect of Gramoxone® Herbicide on Spore Viability and Larvicidal Activity of Bacillus Sphaericus 2362 and 1593 Strains
(Parlar Scientific Publications (p S P), 2003) Berber, I; Atalan, E; Çokmus, C
Spore viability and larvacidal activity of B. sphaericus 2362 and 1593 strains were monitored in media containing Gramaxone(R) herbicide. Minimal Inhibitory Concentrations (MICs) of the herbicide were determined for each of the mosquito pathogenic strains. Addition of paraquat into the media suppressed the spore germination after 24 and 48 h, but no influence was observed on parasporal inclusions containing insecticidal toxin proteins. SDS-PAGE analysis confirmed the presence of the so-called binary toxin comprising two proteins that are deposited in parasporal crystals treated with the herbicide. Moreover, the number of heat-resistant spores and total cell numbers of test strains were reduced drastically when test strains were grown in media containing 50, 100 and 200 mg ml(-1) of paraquat. In contrast, there were no differences between heat-resistant spore counts of both strains when grown under diet concentrations of less than 50 mg ml-1. This study revealed that B. sphaericus 2362 strain has a higher mosquito larvacidal activity than 1593 strain in the presence of paraquat in the media. Thus, the use of Gramaxone(R) herbicide can have harmful effects on mosquitocidal biological control agents.
Effects of Dexamethasone on Trace Elements and Serum Protein Patterns Following Brain Trauma in Rats
(Humana Press inc, 2005) Ekin, S; Berber, I; Kiymaz, N
The effect of dexamethasone (Dxm) on trace elements and serum protein patterns was investigated in male Sprague-Dawley rats subjected to brain trauma. After 6- or 24 h of the traumatic incident, the level of serum copper was significantly higher in the Dxm-treated rats, compared to controls (p < 0.05). The corresponding levels of zinc and iron did not show significant differences. The zinc level returned to normal 24 h after trauma. After 6 and 24 h of trauma, the sodium dodecyl sulfate (SDS)-polyacrylamide gel patterns of serum proteins showed that a 41.6-kDa protein was significantly increased in the Dxm-treated animals. Two proteins weighing 26.6 kDa and 55.1 kDa did not show Dxm-induced changes. These results suggest that increases in the copper-zinc ratio and the changes of the 26.6-kDa, 41.6-kDa, and 55.1-kDa proteins might be a useful prognostic indicator for severe traumatic brain injury.
Effects of Uv-Light on Bacillus Sphaericus and Its Protection by Chemicals
(Wiley-v C H verlag Gmbh, 2000) Çökmüs, C; Sayar, AH; Saçilik, SC; Osmanagaoglu, Ö; Berber, I
Although when UV-irradiated seven most toxic strains of Bacillus sphaericus lost their viabilities between 2.5-4.5 min, their larvicidal activity was protected for longer periods. Benzaldehyde, cinnamaldehyde, salicylaldehyde, methylene blue and yeast extract showed good protective effect for spore viability and larvicidal activity from UV inactivation in B. sphaericus. This protective effect has also been confirmed by SDS-PAGE analyses whereby the 42 kDa and 51 kDa toxic proteins bands did not disappear following UV treatment.
The Influence of Pesticides on the Spore Viability, Toxin Stability and Larvicidal Activity of Bacillus Sphaericus 2362 Strain
(Parlar Scientific Publications (p S P), 2004) Berber, I; Atalan, E; Cokmus, C
In this study, 12 different commercial pesticides, including 5 fungicides, 5 herbicides, 1 aphicide and 1 acaricide, were investigated for their effects on spore viability, toxin stability and larvicidal activity of a mosquito pathogenic B. sphaericus 2362 strain. Minimal inhibitory concentrations (MICs) of each pesticide were determined after 48 h incubation of B. sphaericus 2362 strain. According to MIC results, all pesticides showed various inhibitory effects on the growth of the test bacterium. Particularly, the pesticides which contain TCA, hymexazol, copper sulphate, hexythiazox, 2,4-D Amine, copper inhibited spore germination significantly and bacterial growth even at the lowest tested concentrations. Our results indicated that various commercial pesticides in different concentrations reduced the spore viability significantly and heat-resistant spore numbers of B. sphaericus 2362 strain. In general, the pesticides decreased the spore numbers 10(4)-10(5)-fold compared to initial ones. Furthermore, it was observed that the larvicidal activity was completely lost when it was grown at the concentration of MIC values or higher. Indeed, SDS-PAGE analysis confirmed that the binary toxin proteins, 41.9 kDa and 51.4 kDa, were lost at the MIC values and higher concentrations although parasporal crystals did not disappear in the much lower pesticide concentration.
Synthesis, Antibacterial and Antifungal Activity of Some New Pyridazinone Metal Complexes
(Elsevier France-editions Scientifiques Medicales Elsevier, 2006) Sönmez, M; Berber, I; Akbas, E
The new various metal complexes of 5-benzoyl-4-hydroxy-2-methyl-6-phenyl-2H-pyridazin-3-one were synthesized. All the complexes were evaluated for their antimicrobial activities against Gram-positive, Gram-negative bacteria and fungi using microdilution procedure. The Cd(II) and Ni(II) complexes exhibited selective and effective activities against one Gram-positive bacterium (Staplylococcus aureus ATCC 6538), one Gram-negative bacterium (Pseudonionas putida ATCC 12633) and against two yeast (Candida albicans ATCC 27541 and Candida tropicalis 1828) in contrast to poor activity observed other microorganisms. The new synthesized complexes were characterized using IR, H-1-NMR and UV spectral data together with elemental analysis. (c) 2005 Elsevier SAS. All rights reserved.