Browsing by Author "Berber, I."

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The Changes in the Serum, Liver, Kidney Protein and Genomic Dna Profiles in Rats Treated With 7,12-Dimethylbenz(a) Anthracene and Plantago Major L
(2014) Berber, I.; Ekin, S.; Levent, A.; Oto, G.
In the study, the changes in the serum, liver, kidney protein and genomic DNA profiles in Wistar albino rats administrated to 7,12-dimethylbenz(a)anthracene (DMBA) and Plantagomajor L. were investigated. SDS-PAGE profiling of the serum proteins showed that the levels of two proteins molecular weighing 140.8 kDa and 46.8 kDa were significantly lower on the 0th day of treatment than on the 60th day of application in the groups. Furthermore, two proteins (34.5 and 22.3 kDa) were solely present in DMBA-treated liver cell lysates. On the contrary, the kidney protein profiles did not show significant banding variations in groups. Genomic DNA analysis also confirmed that DNA extracted from liver and kidney cells did not fragment in any of the groups after the 60th study day. The proteins 34.5 and 22.3 kDa in liver cell lysates at the 60th day in DMBA-treated group could be extra-prognostic indicators for severe DMBA toxicity.
Effect of Pesticides on the Growth of Bacillus Thuringiensis Var. Israelensis and Bacillus Sphaericus 2362 Strain
(2003) Berber, I.; Atalan, E.; Cokmus, C.
In this study, twenty-three different commercial pesticides including nine insecticides, six herbicides, four fungicides, three plant growth regulators and one acaricide were investigated for their effects spore viability and larvicidal activity of mosquito pathogenic Bacillus thuringiensis var. israeletisis and Bacillus sphaericus 2362 strain. Minimal inhibitor concentration (MIC) for each pesticide was determined for test bacteria grown into nutrient yeast salt (NYSM) broth. All pesticides except four had an adverse impact on bacterial growth, according to the MIC values. The most inhibitory insecticide, herbicide, fungicide and plant growth regulator were endosulfan 62.5 μL mL-1, diclofop-methyl 28 EC 62.5 μ mL-1, copper sulphate 12.5 μg mL-1 and gibberellic acid 0.25 mL mL-1, respectively. Neoron 500 EC, an acaricide, 31.2 μ mL-1 was the most inhibiting of all pesticides. In general, there was no significant difference between the susceptibility of Bacillus thutbigiensis var. israelensis and Bacillus sphaericus 2362 strain for pesticides examined in this study. High concentrations of all pesticides reduced heat-tolerant spore samples by suppressed spore germination. No larvacidal activity was observed at the MIC values or higher. These results should be considered for assessing the MIC value of these pesticides when both microbial pest agents used in field application. © International Scientific Publications, New Delhi.
Investigation of Some Physiological and Biochemical Parameters in Pseudomonas Syringae-Infected Tomato Plants
(Asian Journal of Chemistry, 2010) Berber, I.; Onlu, H.; Ekin, S.; Battal, P.; Erez, M. E.
In the present study, the levels of manganese, magnesium, plant hormones (indole-3-acetic acid and abscisic acid), sugars (fructose and maltose), total chlorophyll and carotenoid contents in tomato plants following inoculation with Pseudomonas syringae pv. tomato were investigated. The trace element analysis showed that the levels of Mn significantly decreased in bacterium-infected tomato plants, comparing to the healthy plants. In contrary, the content of Mg in the bacterium-infected plants was higher than the uninfected plants. Manganese deficient in the bacterium-infected plants might be an important indicator against attempted bacterial infection. The results of phytohormone analysis showed that there may be an opposite relationship between the concentrations of endogenous indole-3-acetic acid and abscisic acid and the enhance disease resistance in bacterium-infected tomato plants. Therefore, it seems that indole-3-acetic acid, like abscisic acid, acts as a negative regulator of plant defense. Fructose concentrations increased in second, fourth, eight days after treatment with pathogen, but there was a decrease in tenth days. However, maltose levels decreased in all the periods after inoculation with the pathogen bacterium comparing to the control healthy plants. These results were also affirmed to the reduction in the total contents of chlorophyll and an increase of total carotenoids in the bacterium infected-plants. The investigation confirmed that there are complex relationships among trace element levels, endogenous plant hormone and sugar in the regulation of defense mechanisms against attacks by bacterial pathogens.
Synthesis and Antibacterial Activity of 4-Benzoyl Pyrazole-3 Acid and Derivatives
(2005) Akbas, E.; Berber, I.; Sener, A.; Hasanov, B.
Some new 1H-pyrazole-3-carboxylic acid and pyridazinone derivatives were synthesized and evaluated for their antibacterial activities against Bacillus cereus ATCC 7064, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 4230 and Pseudomonas putida using tube dilution method. The minimal inhibitory concentrations (MICs) experiments revealed that all chemical compounds showed inhibitor effects on the growth of the test microorganisms. Moreover, the results of this research showed that the compound named as 5c was the best compound in the series, exhibiting antibacterial activity against both Gram-positive and Gram-negative bacteria. © 2004 Published by Elsevier SAS.
Use of the Different Polyacrylamide Gel Electrophoresis (Page) Methods in the Characterization of Human Pathogenic Staphylococcus Aureus Strains
(Chemical Publishing Co., 2010) Berber, I.; Alan, N.; Ekin, S.; Onlu, H.
In the study, a total of 41 strains of Staphylococcus aureus including 38 clinical isolates and 3 reference strains were characterized according to biotyping, plasmid profiles and the numerical analysis of the protein profiles. The antibiogram results showed that the isolates were resistant against the tested antibiotics, except for vancomycin. Furthermore, plasmid profile analysis confirmed that the strains consisted of only one plasmid. Present findings indicated that the whole-cell and extracellular protein profiles obtained by using SDS-PAGE methods to be good typing tool for the differentiation of S. aureus strains at the species level, not strain level. However, Native-PAGE of whole-cell proteins was the most reliable and rapid method for differentiation between MRSA and ordinary S. aureus strains comparing to other applying PAGE techniques. In addition, it was determined that the same clone was responsible for most cases of MRSA and other S. aureus epidemic among surveyed hospitals. In conclusion, this study supposed that together application at least two different polyacrylamide gel electrophoresis (PAGE) techniques can be offer accurate and an effective approach to the investigation of taxonomic relationships within human pathogenic S. aureus strains.