Browsing by Author "Berkoz, M."

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Anti-Inflammatory Effects of Usnic Acid in Breast Cancer
(Maik Nauka/interperiodica/springer, 2022) Yildirim, M.; Degirmenci, U.; Akkapulu, M.; Gungor, M.; Oztornaci, R. O.; Berkoz, M.; Yalin, S.
cancer is the most common type of cancer among women. Usnic acid has anticancer and anti-inflammatory potential. In this study, we evaluated the effects of usnic acid on inflammation and oxidative stress in the breast cancer cell line (MCF-7). Cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) gene expression, cytokines, nitric oxide (NO), prostaglandin E2 (PGE2) levels and oxidative stress were measured in all groups. Our study showed that usnic acid was decreased NO, VEGF, PGE2 levels, gene expression levels of COX-2 and iNOS in the meantime cytokines (IL 2, CXCL 10, CXCL8, CCL2 (MCP-1), TNF-alpha, IL-6), glutathione levels, moreover decreased GSH and increased MDA levels in dose depended manner. According to our evidence usnic acid was showed potential cytotoxic, anti-inflammatory and pro oxidant role.
Anti-Inflammatory Potential of Thymoquinone in Tumor Necrosis Factor-Alpha Stimulated Sw982 Human Synovial Fibroblasts
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Berkoz, M.; Yunusoglu, O.; Krosniak, M.; Francik, R.
Rheumatoid arthritis is a common systemic autoimmune disease characterized by chronic inflammation of the joints that can induce the formation of pannus tissue and ultimately leads to joint destruction. Thymoquinone, the major bioactive constituent of Nigella sativa seed oil has diverse pharmacological properties. Although there are some studies in the literature showing the anti-inflammatory activity of thymoquinone, it is not yet clear whether thymoquinone can prevent inflammation caused by rheumatoid arthritis. The goal of this study was to investigate the potential anti-inflammatory effects of thymoquinone treatment on synovial fibroblast s. In our study, we investigated the effects of thymoquinone on nitric oxide production, interleukine-6 (IL-6), IL-8, and prostaglandin E2 (PGE2) levels, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor receptor-1 (TNF-R1), and TNF-R2 protein expressions, extracellular signal-regulated protein kinases 1 and 2 (ERK1/2), Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK), and Akt phosphorylation levels in tumor necrosis factor-alpha (TNF-α) stimulated SW982 human synovial fibroblasts. Thymoquinone treatment (0-1 µM) resulted in significant and concentration-dependently reduced the TNF-α stimulated production of nitric oxide, IL-6, IL-8, and PGE2 levels compared to the untreated group (p<0.05). Also thymoquinone treatment in high concentrations exerted an anti-inflammatory effect by suppressing iNOS, COX-2, TNF-R1, and TNF-R2 protein expressions and the phosphorylation of JNK, p38 MAPK, ERK1/2 and Akt in SW982 synovial fibroblasts (p<0.05). Taken together, these results show that thymoquinone in high concentrations is able to play a beneficial role in TNF-α mediated signaling in rheumatoid arthritis synovial fibroblasts. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effect of Capsaicin on Transcription Factors in 3t3-L1 Cell Line
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Berkoz, M.; Yildirim, M.; Arvas, G.; Turkmen, O.; Allahverdiyev, O.
Capsaicin is a spicy ingredient of Capsicum annuum and a lipophilic, crystalline, odorless and colorless alkaloid. Although the effect of capsaicin on adipocyte differentiation is well-known, the role of capsaicin on transcription factors while adipocyte differentiation is not clear. The aim of this study is thus to identify and characterize the transcription factors in the process of adipocyte differentiation after the capsaicin treatment. In this study, concentration of 0, 50, 100, 150, 200 and 250 µM capsaicin were treated to 3T3-L1 pre-adipocytes in cell culture. MTT cell cytotoxicity, cell viability with trypan blue staining, Lactate Dehydrogenase (LDH) enzyme assay, triglyceride content assay, Glycerol-3-Phosphate Dehydrogenase (GPDH) activity, Oil Red O staining and mRNA levels of transcription factors (PPARγ, C/EBPα and SREBP-lc) were investigated in capsaicin induced 3T3L1 preadipocyte cell line. Capsaicin treatment decreased cell population growth of 3T3-L1 preadipocytes, assessed with trypan blue staining, MTT test and rising of LDH release proportion. Capsaicin inhibited GPDH activity and intracellular triglyceride content in 3T3-L1 adipocytes in all treated groups in a dose-dependent manner. Oil Red O staining indicated that capsaicin inhibited adipocyte differentiation in 3T3-L1 adipocytes in all treatment groups. In this study, it was revealed that exposing 3T3-L1 preadipocytes and differentiating postconfluent preadipocytes to different doses of capsaicin decreased PPARγ, C/EBPα and SREBP-1c mRNA levels as compared with their controls without treatment in dose dependent manner. Although, reduction of PPARγ mRNA level was statistical significant, this decrease was not significant in C/EBPα and SREBP-1c mRNA levels. This study demonstrated that capsaicin treatment inhibited the adipogenesis through the down-regulation of transcription factors, especially PPARγ. Alternative mechanisms may involve cell cycle arrest and the induction of apoptosis. Since capsaicin is the main component found in hot pepper, the consumption of hot pepper may contribute to the maintenance of body weight and prevent the development of obesity. © 2015 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effect of Hyperoside on the Inhibition of Adipogenesis in 3t3-L1 Adipocytes
(Editura Acad Romane, 2019) Berkoz, M.
Context. The inhibition of adipocyte differentiation has a significant role on the prevention of obesity and obesity-associated complications. Objective. In this study, we aimed to detect whether hyperoside is able to inhibit the conversion of pre-adiposits into mature adiposits. Design and Methods. 3T3-L1 pre-adipocytes were stimulated so as to differentiate into mature adipocytes. Hyperoside in non-cytotoxic concentrations (1, 2, 5, and 10 mu M) were separately applied to differentiated 3T3-L1 cells. Oil red O staining was performed and triacylglycerol contents were measured. Furthermore, gene and protein expressions of transcription factors, adipogenic genes and adipokines were examined in order to investigate the effect of hyperoside on adipocyte differentiation. Results. Hyperoside in high concentrations significantly suppressed the adipogenic process by inhibiting the expression of transcription factors and adipogenic genes and reducing lipid accumulation in adipocytes (p<0.05). Low doses of hyperoside are able to inhibit adipogenesis, but higher doses are needed to reduce fat accumulation in mature adipocytes. In the case of maturing preadipocytes, 5 mu M of hyperoside exerts its antiadipogenic effect at the early stages of adipogenesis, whereas 10 mu M of hyperoside acts at the later stages (p<0.05). Conclusion. These results suggest that hyperoside has a beneficial effect on the prevention and treatment of obesity.
The Effect of Sitagliptin and Exenatide on Insulin, Insulin Receptor and Insulin Receptor Substrate Gene Expression in Diabetic Rats
(Maik Nauka/interperiodica/springer, 2023) Yildirim, M.; Mistik, O. Agtas; Yalin, A. E.; Comelekoglu, U.; Berkoz, M.; Arpaci, R. Bozdogan; Yalin, S.
Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia that results from abnormal secretion of insulin, inappropriate action of insulin or both. The aim of this study is to determine the effects of exenatide and sitagliptin treatment on the expression of insulin (INS-1), insulin receptor (INSR) and insulin receptor substrate (IRS-1) genes. In this study streptozotocin-induced diabetic rats were treated with sitagliptin (10 mg/kg) and exenatide (0.1 mu g/kg) for 15 days. We measured INS-1, INSR and IRS-1 gene expression levels and examined the histology of pancreatic tissues in all groups. Exenatide and sitagliptin treatment increased gene expression levels of INS-1, INSR and IRS-1.
In Vitro Anti-Obesity Effect of Aloe Vera Extract Through Transcription Factors and Lipolysis-Associated Genes
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Yunusoglu, O.; Türkmen, Ö.; Berkoz, M.; Yıldırım, M.; Yalın, S.
In recent years, obesity has been associated with heart diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancer and osteoarthritis, and become a global health problem. Therefore, researchers seek to find functional drugs against obesity. For the past few decades, medicinal plants have been examined to for their anti-obesity effects, including Aloe vera. In this study, it was aimed to elucidate the inhibitory effect of Aloe vera extract on adipogenesis. Firstly, 3T3-L1 pre-adipocytes were stimulated so as to differentiate into mature adipocyte using adipogenic differentiation cocktail consisting of 10 μg/mL insulin, 0.5 mM isobutylmethylxanthine, 0.25 mM dexamethasone, and 100 ng/mL biotin on day 0. Various concentrations (10-50 µg/mL) of Aloe vera extract with no cytotoxic effect were applied to differentiated 3T3-L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil r ed O staining, intracellular triglyceride levels, and gene expressions of transcription factors and lipolysis-associated genes were examined in order to investigate the effect of Aloe vera extract on adipocyte differentiation. Aloe vera treatment caused a continuous decrease in cell size and intracellular triglyceride accumulation. Despite the fact that GPDH activity, mRNA levels of transcription factors and lipolysis-associated genes decreased in mature adipocytes treated with Aloe vera extract. These results suggest that Aloe vera may have a therapeutic effect in prevention and/or treatment of adipogenesis-related obesity. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Pai-1 and Tpa Gene Polymorphisms and Susceptibility To Chronic Obstructive Pulmonary Disease in a Sample of Turkish Population
(Pakistan Medical Association, 2020) Sunnetcioglu, A.; Berkoz, M.; Yildirim, M.; Bora, G.; Yalin, S.
Objective: The aim of this study was to assess the influence of plasminogen activator inhibitor-1 (PAI-1) 4G/5G or tissue plasminogen activator (tPA) I/D polymorphisms in chronic obstructive pulmonary disease (COPD) cases in a sample of Turkish population. Methods: PAI-1 4G/5G and tPA Alu-repeat I/D genetic polymorphisms in 153 COPD subjects and 160 controls were investigated using PCR-RFLP and PCR methods, respectively. Results: 4G allele frequency was 0.62 and 0.39 for COPD and control groups, respectively. 4G allele had an estimated 2.56-fold [95% CI = 1.85-3.53] increased risk of COPD. tPA I allele frequency was 0.55 and 0.50, for COPD and control groups, respectively. I allele had an estimated 1.19-fold [95% CI = 0.87-1.62] increased risk of COPD Conclusions: PAI-1 4G/4G and 4G/5G genotypes seemed to play a key role in the pathophysiology of COPD in Turkish individuals. © 2020 Pakistan Medical Association. All rights reserved.
Punicalagin and Punicalin Suppress the Adipocyte Differentiation Through the Transcription Factors
(Editura Acad Romane, 2021) Berkoz, M.; Yalin, S.; Yildirim, M.; Yalin, A. E.; Comelekoglu, U.
Background. Pomegranate is a rich source of many polyphenolic compounds including ellagitannins (punicalagin, punicalin and others). Aim. The effects of punicalagin and punicalin on adipogenesis were investigated in this study. Materials and Methods. To examine the effect of punicalagin and punicalin on adipocyte differentiation, various concentrations of punicalagin and punicalin (2-10 mu M) were applied to differentiated 3T3-L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil red O staining, intracellular triglyceride levels, and gene expressions of transcription factors (Peroxisome proliferator-activated receptor-gamma (PPAR gamma), CCAAT-enhancer-binding proteins-alpha (C/EBP alpha), Sterol regulatory element-binding protein 1c (SREBP-1c)) and lipolysisassociated genes (hormone-sensitive lipase (HSL), Perilipin A, tumor necrosis factor-alpha (TNF-alpha)) were examined in order to investigate the effects of punicalagin and punicalin on adipocyte differentiation. Results. Punicalagin and punicalin applications caused a continuous decrease in cell size and intracellular triglyceride accumulation. GPDH activity and transcription gene expressions decreased significantly in groups that were applicated punicalagin and punicalin at high concentrations. Punicalagin, but not punicalin, down-regulated the expression of HSL and perilipin A and up-regulated the expression of TNF-alpha in a dose-dependent manner. In conclusion, both punicalagin and punicalin were able to inhibit the adipocyte differentiation.