Browsing by Author "Berkoz, Mehmet"

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Antioxidant Activities of Curcumin in Allergic Rhinitis
(Springer, 2016) Altintoprak, Niyazi; Kar, Murat; Acar, Mustafa; Berkoz, Mehmet; Muluk, Nuray Bayar; Cingi, Cemal
We investigated the antioxidant effects of curcumin in an experimental rat model of allergic rhinitis (AR). Female Wistar albino rats (n = 34) were divided randomly into four groups: healthy rats (control group, n = 8), AR with no treatment (AR + NoTr group, n = 10), AR with azelastine HCl treatment (AR + Aze group, n = 8), and AR with curcumin treatment (AR + Curc group, n = 8). On day 28, total blood IgE levels were measured. For measurement of antioxidant activity, the glutathione (GSH) level and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities were measured in both inferior turbinate tissue and serum. Malondialdehyde (MDA) levels were measured only in inferior turbinate tissue, and paraoxonase (PON) and arylesterase (ARE) activities were measured only in serum. Statistically significant differences were found for all antioxidant measurements (GSH levels and CAT, SOD, GSH-Px activities in the serum and tissue, MDA levels in the tissue, and PON and ARE activities in the serum) between the four groups. In the curcumin group, serum SOD, ARE, and PON and tissue GSH values were higher than the control group. Moreover, tissue GSH levels and serum GSH-Px activities in the curcumin group were higher than in the AR + NoTr group. In the azelastine group, except MDA, antioxidant measurement values were lower than in the other groups. Curcumin may help to increase antioxidant enzymes and decrease oxidative stress in allergic rhinitis. We recommend curcumin to decrease oxidative stress in allergic rhinitis.
Antioxidant and Anti-Lipoxygenase Activities of Cydonia Oblonga
(2020) Berkoz, Mehmet
The present studies are focused to explore the antioxidant and anti-lipoxygnease activities in crude extract, aqueous and organic fractions (ethyl acetate, chloroform, butanol) of dry fruit of Cydonia oblonga. For determining the antioxidant activity; total phenolic content and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay were performed in the aqueous and organic fractions of C. oblonga. In our study, phenolic content revealed that crude extract has highest phenolic content followed by ethyl acetate fraction. The results of DPPH radical scavenging activity showed IC50 values of crude extract as 61.4 μg/mL, chloroform and aqueous 67.4 and 55.5 μg/mL, respectively, which are well comparable with butylatedhydroxyanisol (BHA) standard, which showed IC50 as 44.2 μg/mL. In addition, crude extract appeared to be the prominent inhibitor of lipoxygenase. The results suggests that the extract and different fractions of dry fruit of C. oblonga exhibits significant antioxidant and free radical scavenging activities/capacity which might be associated with the presence of main phytochemicals like flavonoids, phenols and tannins. Besides that, all these extract and fractions have well enough lipoxygenase inhibitory potential. As a result, we can claim that the presence of the scavenger molecules in the dry fruit of C. oblonga may attribute health benefits by providing the protective effects during the progressive stages of different acute and chronic disorders.
Artesunate Inhibits Melanoma Progression in Vitro Via Suppressing Stat3 Signaling Pathway
(Springer Heidelberg, 2021) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Krosniak, Miroslaw; Turkmen, Omer; Korkmaz, Duygu; Keskin, Siddik
Background Melanoma is a life-threatening cancer characterized with a potentially metastatic tumor of melanocytic origin. Improved methods or novel therapies are urgently needed to eliminate the development of metastases. Artesunate is a semi-synthetic derivative of artemisinin used for trarment of malaria and cancer. The purpose of this study was to investigate the anti-cancer effect of artesunate and the role on STAT3 signaling in A375 human melanoma cell line. Methods Melanoma cells were treated with artesunate at concentrations of 0-5 mu M for 24 and 48 h. The inhibition of cell viability, colony formation, migration, invasion, adhesion, percentage of apoptotic cells, and expressions of signal transducer and activator of transcription-3 (STAT3) and related proteins were examined. Results Artesunate inhibited cellular proliferation of cancer cells by induction of apoptosis at sub-toxic doses. Cells treated with artesunate showed an inhibition in adhesion to extracellular matrix substrate matrigel and type IV collagen. Artesunate treatment showed a decreased cellular migration, invasion, and colony formation in melanoma cells. Artesunate also inhibited STAT3 and Src activations and STAT3 related protein expressions; such as metalloproteinase 2 (MMP-2), MMP-9, Mcl-1, Bxl-xL, vascular endothelial growth factor (VEGF), and Twist. Moreover, overexpression of constitutively active STAT3 in A375 cells attenuated the anti-proliferative, apoptotic and anti-invasive effects of artesunate. Conclusion The results obtained from this study demonstrated that the anticancer activity of artesunate occurred via STAT3 pathway and its target proteins. Therefore, it can be suggested that artesunate may be an important candidate molecule in the treatment of melanoma.
Assessment of Liver Volume With Computed Tomography and Comparison of Findings With Ultrasonography
(Springer, 2014) Bora, Aydin; Alptekin, Cem; Yavuz, Alpaslan; Batur, Abdussamet; Akdemir, Zulkuf; Berkoz, Mehmet
In this study, we aimed to investigate the impact of non-alcoholic hepatic steatosis on the liver volume. As investigating hepatic steatosis, we utilized computed tomography (CT) to determine the degree of steatosis and we utilized hepatobiliary ultrasonography (USG) for densitometry and correlation. As hepatosteatosis group, 35 patients over 18 years of age and whose abdominal CT scans were requested by several clinics and performed routinely were included in this study, and as control group, 40 healthy subjects without hepatosteatosis (clinically and radiologically) and correlated with hepatosteatosis group in terms of age and gender were included in this study. CT densitometry and liver attenuation index (LAI) of all individuals who participated in our study were calculated, and contrast images of patients were transferred to CT-Volume Software (Siemens Syngo Multimodality Workplace; Version VE52A). In this study, interactive and automated volume measurement techniques were used together. The volumes were measured separately in patient and control group. In this study for each stage in USG, there was found a direct correlation in terms of LAI and volume, and this correlation was statistically significant (p < 0.01). Furthermore, statistical significance between size and USG stage draws attention (p < 0.05). A significance relationship between USG stage and age could not be determined. As a result, we have reached the conclusion that CT densitometry can be used as an assistive technique along with USG to determine the degree of steatosis in the non-alcoholic fatty liver disease, and there is a positive linear correlation between the liver size and volume, and liver volume increases in the non-alcoholic fatty liver disease.
Association Between Il-4 Gene Polymorphisms and Il-4 Serum Levels in Patients With Allergic Rhinitis
(2021) Berkoz, Mehmet; Çetin, Yaser Said; Düzenli, Ufuk; Bozan, Nazım; Özkan, Hüseyin
Genetic factors play a major role in the formation of allergic rhinitis. The most studied genes for the investigation of the genetic origin of allergic rhinitis are the cytokine genes. We aimed to analyse the relation between susceptibility to allergic rhinitis and IL-4 C-590T and C+33T polymorphisms. For this aim, serum IL-4 levels and IL-4 C-590T and C+33T polymorphisms of 211 allergic rhinitis cases and 232 healthy individuals (control group) were analysed. We found that the individuals carrying IL-4 +33T allele and IL-4 -590TT and +33TT genotypes were much more predisposed to allergic rhinitis than the individuals carrying CC wild type genotypes. Our results also suggest that serum IL-4 levels of control group carrying the IL-4 -590 CT and TT genotypes and T allele and IL-4 +33 CT and TT genotypes, and T allele were significantly higher than the CC genotypes and T alleles carriers. As a result, it is possible to say that IL-4 C-590T and C+33T polymorphisms may increase the susceptibility of allergic rhinitis because of their lowering of IL-4 levels in serum.
Association Between Pon1 L55m Polymorphism and Pon1 Enzyme Activity in Patients With Leukemia
(Akad Doktorlar Yayinevi, 2017) Eras, Nazan; Tombak, Anil; Tiftik, Naci; Yalin, Serap; Berkoz, Mehmet; Kul, Seval; Akbas, Etem
Paraoxonase 1 (PON1) is an important antioxidant enzyme which has a role in preventing the effects of systemic oxidative stress. The purpose of our study was to investigate the possible association between PON1 L55M polymorphism and leukemia development and to determine the relationship between PON1 genotypes and PON1 enzyme activities. Genotypes of 102 cases and 112 healthy controls were determined by PCR-RFLP. PON1 enzyme activity was determined according to Eckerson's method. The ratio of MM genotype belonging to PON1 L55M polymorphism in control group was 6.3% and was 7.8% in patients with leukemia (p= 0.39). PON1 enzyme activity was 118.8 +/- 115.1 U/mL in control group, while decreased to 75.6 +/- 64.4 U/mL in patients with leukemia (p= 0.004). PON1 enzyme activities of the individuals with MM genotypes belonging to PON1 L55M polymorphism was 57.43 21.61 U/ mL in control group and decreased to 39.18 +/- 45.61 U/mL in leukemic patients. Our results suggest that, PON1 L55M polymorphism genotype ratios do not affect leukemia development. However, reduced PON1 enzyme activity and also the combination of PON1 L55M polymorphism with reduced PON1 enzyme activity are associated with the increased risk of leukemia. Furthermore, older age may be a risk factor for developing leukemia.
Boswellic Acid and Betulinic Acid Pre-Treatments Can Prevent the Nephrotoxicity Caused by Cyclophosphamide Induction
(Maik Nauka/interperiodica/springer, 2024) Berkoz, Mehmet; Ciftci, Oguzhan
Cyclophosphamide (CYP) is a chemotherapeutic drug used to treat various cancers. However, its clinical use is limited due to severe organ damage, particularly to the kidneys. While several phytochemicals have been identified as potential therapeutic targets for CYP nephrotoxicity, the nephroprotective effects of boswellic acid (BOSW) and betulinic acid (BET) have not yet been investigated. Our study used 42 rats divided into six equal groups. The study included six groups: control, CYP (200 mg/kg), CYP+BOSW20 (20 mg/kg), CYP+BOSW40 (40 mg/kg), CYP+BET20 (20 mg/kg), and CYP+BET40 (40 mg/kg). The pre-treatments with BOSW and BET lasted for 14 days, while the application of cyclophosphamide was performed intraperitoneally only on the 4th day of the study. After the experimental protocol, the animals were sacrificed, and their kidney tissues were isolated. Renal function parameters, histological examination, oxidative stress, and inflammation parameters were assessed both biochemically and at the molecular level in kidney tissue. The results showed that oxidative stress and inflammatory response were increased in the kidney tissue of rats treated with CYP, leading to impaired renal histology and function parameters (p < 0.05). Oral administration of both doses of BET and especially high doses of BOSW improved biochemical, oxidative, and inflammatory parameters significantly (p < 0.05). Histological studies also showed the restoration of normal kidney tissue architecture. BOSW and BET have promising biological activity against CYP-induced nephrotoxicity by attenuating inflammation and oxidative stress and enhancing antioxidant status.
Can Juniperus Communis L. Oil Improve Nephropathy in Diabetic Rats
(Marmara Univ, inst Health Sciences, 2020) Kahraman, Tahir; Berkoz, Mehmet; Allahverdiyev, Oruc; Mahmood, Evan Abdulkarim; Yildirim, Metin; Yalin, Serap
Objective: Juniperus communis L. (J. communis) is a shrub belonging to family Cupressaceae L. mainly growth in Eurasia. The antioxidant and antidiabetic activity of aqueous extract of J. communis L. berries indicated benefits as a potent antidiabetic in streptozotocin induced diabetic albino rats. This study was carried out to determine whether J. communis L. oil supplement will effectively manage renal dysfunction in diabetic rats. Methods: Twenty eight rats were divided into 4 equal groups as follows; control group, diabetic group (45 mg/kg, i.p. streptozotocin), J. communis L. oil (200 mg/kg) treated group, and diabetic+J. communis L. oil (200 mg/kg) treated group. At the end of the experimental period, all rats were sacrificed and renal function parameters such as kidney antioxidant and lipid peroxidation markers and serum glucose, HbA1c, creatinine, serum urea, blood urea nitrogen (BUN), and serum total protein levels were measured in all groups. Results: HbA1c, serum glucose, urea, creatinine, BUN and, kidney lipid peroxidation levels increased (p<0.05), but serum total protein and antioxidant levels decreased in diabetic group comparing with control group (p<0.05). Furthermore, HbA1c, serum glucose, urea, creatinine and BUN and, kidney lipid peroxidation levels decreased and also, serum total protein and antioxidant levels increased in diabetic group treated with J. communis L. oil comparing with diabetic group (p<0.05). Conclusion: This study has provided that J. communis L. oil provide a protective effect on the kidney as evidenced by an improvement of the renal function tests as well as reduction in oxidative stress parameters in experimental diabetic nephropathy model.
Correlation Between Gsh-Px Pro198leu, Cat-262c/T, Mnsod Ala16val Gene Polymorphisms and Allergic Rhinitis
(Galenos Publ House, 2022) Kundi, Pinar; Bozan, Nazim; Berkoz, Mehmet; Cankaya, Hakan
Introduction: In this study, we investigated the etiopathogenesis of allergic rhinitis by analyzing the polymorphisms including GPx-1 Pro198Leu, CAT-262 C/T, and MnSOD Ala16Val. Methods: The diagnosis of allergic rhinitis was diagnosed by clinical history, examination, serum total immunoglobulin E levels and skin prick test. Five mL of peripheral blood from patients and individuals constituting the control group was taken into EDTA tubes. DNA isolation from whole blood samples was performed according to the Poncz method. Results: Because of this study; for the Pro198Leu polymorphism of the GPX1; was concluded with 95% confidence that the presence of the Leu allele increased the susceptibility to allergic rhinitis 1.092 times. However, this increase was not found to be statistically significant. For the -262 C/T polymorphism of the CAT gene; was concluded with 95% confidence that the presence of the T-allele increased the susceptibility to allergic rhinitis 27,064 times. This increase was found to be statistically significant. For Ala16Val polymorphism of the Mn-SOD gene; was concluded with 95% confidence that the presence of the Ala allele increased the susceptibility to allergic rhinitis 25,791 times. This increase was found to be statistically significant. Conclusion: A significant relationship was found between allergic rhinitis and the genotypes and the frequencies of alleles in the polymorphisms of the MnSOD and CAT genes. However, no significant relationship was found between allergic rhinitis and the polymorphisms of the GPx-1 gene.
Deneysel Osteoporoz Modeli Oluşturulmuş Ratlarda Stronsiyum Ranelat Uygulanmasının Karaciğer, Böbrek ve Kas Dokularındaki Paraoksonaz ve Arilesteraz Aktivitelerine Etkisinin Araştırılması
(2017) Berkoz, Mehmet; Yalın, Serap; Sogut, Fatma; Eroğlu, Pelin; Sağır, Özgün; Çömelekoğlu, Ülkü
Amaç: Yaptığımız bu çalışmada deneysel osteoporoz modeli oluşturulan sıçanlarda, post-menopozal tedavide yaygın olarak kullanılan stronsiyum ranelatın karaciğer, böbrek ve kas dokularındaki paraoksonaz (PON) ve arilesteraz (ARE) enzim aktiviteleri üzerindeki etkisi araştırılmıştır. Gereç ve Yöntem: Bu çalışmada 35 adet dişi Wistar Albino sıçan kullanılmış ve her bir grupta 7 hayvan olacak şekilde 5 gruba ayrılmıştır. Bu gruplar kontrol grubu (Grup I), 3 ay boyunca sadece stronsiyum ranelat uygulanan grup (Grup II), sadece ooferektomi uygulanan grup (Grup III), ooferektomi sonra profilaktik amaçla hemen stronsiyum ranelat tedavisine başlanan grup (Grup IV) ve ooferektomi yapıldıktan 3 ay sonra stronsiyum ranelat tedavisine başlanan grup (Grup V) olarak belirlenmiştir. Tüm gruplardaki hayvanların karaciğer, böbrek ve kas dokuları izole edilerek PON ve ARE aktivitelerine bakılmıştır. PON ve ARE değerleri bakımından gruplar arasındaki istatistiksel farklılıklar ANOVA ve alt grup karşılaştırmalarında Tukey çoklu karşılaştırma testleri kullanılarak değerlendirilmiştir. Bulgular: Yaptığımız çalışmada tüm grupların karaciğer, böbrek ve kas PON ve ARE aktiviteleri kontrol grubuna kıyasla anlamlı ölçüde düşük olduğu tespit edilmiştir (p<0,05). Ayrıca Grup V'in karaciğer PON ve ARE aktivitelerinin Grup II, Grup III ve Grup IV'e göre anlamlı ölçüde yüksek olduğu tespit edilmiştir (p<0,05). Sonuç: Bulunan sonuçlar postmenopozal dönemin ve stronsiyum ranelat kullanımının karaciğer, böbrek ve kas dokularındaki PON ve ARE aktivitelerini azalttığını, dolayısıyla antioksidan sistemi zayıflattığını göstermektedir.
Diosmin Suppresses the Proinflammatory Mediators in Lipopolysaccharide-Induced Raw264.7 Macrophages Via Nf-Κb and Mapks Signal Pathways
(General Physiol and Biophysics, 2019) Berkoz, Mehmet
Diosmin is an unsaturated flavonoid glycoside, presents in citrus fruits. The aim of this study is to investigate the molecular mechanism of diosmin with respect to the NF-kappa B and MAPKs signaling pathways. Firstly, 10, 20, 30, 40 and 50 mu M diosmin were treated to lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The anti-inflammatory effects of diosmin was displayed via measuring prostaglandin E-2 (PGE(2)), nitric oxide (NO), interleukines (IL-6, IL-12), tumor necrosis factor alpha (TNF-alpha) production, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), IL-6, IL-12, TNF-alpha mRNA levels, and phosphorylation levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (I kappa B-alpha) and mitogen-activated protein kinases (MAPKs); JNK, ERK, and p38 in LPS induced RAW264.7 macrophages. Our study showed that especially high concentrations of diosmin decreased NO, PGE 2 , IL-6, IL-12, TNF-alpha production and mRNA levels of these mediators (p < 0.05). The expression of phosphorylated-JNK was significantly suppressed by diosmin at 40 and 50 mu M concentrations. Furthermore, diosmin significantly inhibited the expression of phosphorylated-ERK, p38, and p-I kappa B-alpha in a dose-dependent manner. Our results suggest that diosmin is a potent anti-inflammatory agent and has potential for development into a therapeutic agent for inflammation-associated disorders.
Effect of Dietary Supplementation of Inulin on Growth Performance, Digestion Enzyme Activities and Antioxidant Status of Rainbow Trout (Oncorhynchus Mykiss)
(2019) Yıldırım, Metin; Çetinkaya, Mükerrem; Hunt, Arzu Ozluer; Yılmaz, Ferbal Özkan; Berkoz, Mehmet; Yalın, Serap
The present study investigated the effects of the prebiotic inulin on the growth parameters, digestiveenzyme activities and antioxidant status of rainbow trout (Oncorhynchus mykiss). After acclimation,fish (34±0.22 g; n = 135) were assigned into 9 tanks (15 fish per tank) and triplicate groups were feda control diet (G-0) or diets containing 1% (G-1) or 2% (G-2) inulin. At the end of the trial (8 weeks),growth factors (final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR),protein efficiency ratio (PER), Economic conversion ratio (ECR), digestive enzyme activities(pepsin, trypsin, amylase and lipase) and antioxidant status superoxide dismutase (SOD), catalase(CAT) and malondialdehyde (MDA) level of liver tissue were assessed. At the end of the studyhighest survival rate was observed in the fish fed with 1% G-1 inulin group. Similarly, digestiveenzyme activities were significantly higher dietary G-1 group. Subsequently, elevated growthperformance (final weight, SGR and FCR) was observed in trout fed with 1% compared to thecontrol group. The effect of the dietary inulin on digestion was partly observed by assaying theactivity of pepsin, intestinal amylase, trypsin and lipase. Apart from pepsin activity, results indicated that dietary supplementation of inulin is beneficial and may also have differential effects upon digestive enzyme activities. Antioxidant enzyme activity of SOD and CAT in liver tissue was generally higher in the supplemented inulin diets than in the control and significantly higher in the 1% inulin group. A significant decrease in MDA level of tissue was observed in all inulin supplemented groups when compared to the control group. These results indicate that inulin be considered as a beneficial dietary supplement for growth performance, digestive enzyme activities and antioxidant status of rainbow trout.
Effects of Dietary Nucleotide Yeast on Immune Responses and Antioxidant Enzyme Activities of Rainbow Trout Juveniles (Oncorhynchus Mykiss)
(Israeli Journal of Aquaculture-bamidgeh, 2016) Ozluer-Hunt, Arzu; Ozkan-Yilmaz, Ferbal; Berkoz, Mehmet; Engin, Kenan; Gunduz, Suna Gul; Yalin, Serap
This study aimed at demonstrating the effects of dietary supplementation of nucleotide yeast base protein (Nu-Pro (R)) (NP) on the antioxidant enzyme activities and immune response in liver and blood tissues of rainbow trout (Oncorhynchus mykiss). Fish with an average initial weight of 27.75 +/- 0.26 g were randomly assigned to four groups with three replicates. Throughout the 60 day grow-out period the control group was fed a fish meal based basal diet, and three other groups were fed diets in which 20 (NP 20), 40 (NP 40) and 60 % (NP 60) fish meal was substituted with nucleotide (Nu-Pro (NP) yeast). There were no significant changes of superoxide dismutase (SOD) and catalase (CAT) activities in liver among the experimental groups. A significant decrease (P<0.05) in malondialdehyde (MDA) level of tissue was observed in all nucleotide supplemented groups when compared to the control group. Serum lysozyme (LYZ) and myeloperoxidase (MPO) activities and nitric oxide (NO) level of liver tissue were significantly (P<0.05) increased in fish fed with nucleotide yeast based protein diets. The results showed that the fish in all nucleotide supplemented groups showed significantly better antioxidant activity and immune responses.
Effects of Dietary Selenium of Organic Form Against Lead Toxicity on the Antioxidant System in Cyprinus Carpio
(Springer, 2014) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap
In this study was evaluated potential protective effect of organic selenium (Se) on heavy metal stress induced by lead (Pb) in Cyprinus carpio. For this reason, C. carpio was exposed to sublethal concentration of Pb (1.5 mg/L Pb(NO3)(2)) for 14 days. The fish were fed a basal (control; measured 0.55 mg/kg Se) diet or a basal diet supplemented with 2.50 mg/kg (measured 2.92 mg/kg Se) organic Se (Sel-Plex(A (R))) during the experiment period. The variations in glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) activities, and levels of reduced glutathione (GSH) with malondialdehyde (MDA) in liver and brain tissues of C. carpio were investigated in experimental groups. GSH levels in liver and brain tissues were significantly decreased by exposure to Pb. GST activity was significantly increased (p < 0.05) in liver tissue, but decreased in brain of treated fish by exposure to Pb. Also, GSH-Px activity was significantly increased in liver tissue, but decreased in brain of Pb-treated fish. Levels of MDA were increased in liver and brain of Pb-treated fish. The organic Se treatment for Pb-intoxicated animals improved activities of GSH-Px, GST and levels of MDA within normal limits. Supplemented Se could be able to improve Pb-induced oxidative stress by decreasing lipid peroxidation and regulating antioxidant defense system in tissues.
Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)
(Parlar Scientific Publications (p S P), 2017) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap; Yildirim, Metin
In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.
Effects of Fisetin on Ethanol-Induced Rewarding Properties in Mice
(Taylor & Francis inc, 2024) Yalniz, Yasin; Yunusoglu, Oruc; Berkoz, Mehmet; Demirel, Mustafa Enes
Background: Alcohol use disorder (AUD) is a chronic relapsing disorder associated with compulsive drinking of alcohol. Natural flavonoid fisetin affects a variety of transmitter systems relevant to AUD, such as aminobutyric acid, N-methyl-D-aspartate, and dopamine, as well as peroxisome proliferator-activated receptors.Objectives: This study investigated fisetin's impact on the motivational properties of ethanol using conditioned place preference (CPP) in mice (n = 50).Methods: Mice were conditioned with ethanol (2 g/kg, i.p.) or saline on alternating days for 8 consecutive days and were given intragastric (i.g.) fisetin (10, 20, or 30 mg/kg, i.g.), 45 min before ethanol conditioning. During extinction, physiological saline was injected to the control and ethanol groups, and fisetin was administered to the fisetin groups. To evaluate the effect of fisetin on the reinstatement of ethanol-induced CPP, fisetin was given 45 min before a priming dose of ethanol (0.4 g/kg, i.p.; reinstatement test day).Results: Fisetin decreased the acquisition of ethanol-induced CPP (30 mg/kg, p < .05) and accelerated extinction (20 and 30 mg/kg, p < .05). Furthermore, fisetin attenuated reinstatement of ethanol-induced CPP (30 mg/kg, p < .05).Conclusions: Fisetin appears to diminish the rewarding properties of ethanol, as indicated by its inhibitory effect and facilitation of extinction in ethanol-induced CPP. These findings imply a potential therapeutic application of fisetin in preventing ethanol-seeking behavior, promoting extinction, and reducing the risk of relapse.
The Effects of Fish Meal Replacement by Yeast Based Nucleotides on Growth, Body Composition and Digestive Enzyme Activity in Rainbow Trout Juveniles (Onchorchyncus Mykiss)
(Israeli Journal of Aquaculture-bamidgeh, 2014) Hunt, Arzu Ozluer; Yilmaz, Ferbal Ozkan; Engin, Kenan; Berkoz, Mehmet; Gunduz, Suna Gul; Yalin, Serap; Sahin, Nefise Ozlen
This 60 day study investigated the effects of organically certified nucleotide yeast-derived protein source (Nu-Pro (R)) on growth, feed efficiency, fillet proximate composition and digestive enzymes in rainbow trout (Onchorhyncus mykiss). Diets were isonitrogenous and isocaloric. Three experimental diets wherein 20%, 40% and 60% of the fish meal content was replaced by Nu-Pro (R), were compared to the control diet in which the crude protein content was anchovy fish meal and corn gluten meal. The rainbow trout (initial weight 27 g/fish) were fed twice daily in 200-l Aqaria. Live weight gain increase ranged from 125-195% in fish fed the experimental diets. Results indicated that up to 40% fish meal protein can be replaced by Nu-Pro (R) without compromising growth rates, feed efficiency or the fillet biochemical composition in the rainbow trout. The effect of the dietary Nu-Pro (R) supplementation on digestion was partly observed by assaying the activity of pepsin, intestinal amylase, trypsin and lipase. Apart from amylase activity, results indicated that dietary supplementation of nucleotides is beneficial and may also have differential effects upon digestive enzyme activities.
Effects of Methidathion on Antioxidant System and Expression of Heat Shock Protein 70 (Hsp70) Gene in the Liver of Oreochromis Niloticus L. 1758
(Parlar Scientific Publications (p S P), 2015) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap; Sahin, Nefise Ozler
In this study, effects of sublethal concentrations of methidathion on catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) activities and lipid peroxidation with expression of heat shock protein (HSP70) gene in liver tissues of Oreochromis niloticus were investigated. The 96-h LC50 value for methidathion was determined as 0.1045 mg/L for O. niloticus in this study. 0.013 (1/8 of LC50) and 0.026 mg/L (1/4 of LC50) sublethal concentrations were applied for 4 and 10 days in this experiment. CAT and GSH-Px activities in liver tissue were increased, in relation to both the time and doses applied. SOD activities were increased under the influence of both concentrations at the 4th day, but on 10th day, it was decreased with influence of 0.026 mg/L methidathion concentration. The tissue MDA levels were significantly increased in relation to both the time and dose applied. The relative mRNA levels of the HSP70 gene were detected by real time polymerase chain reaction (RT-PCR). The mRNA levels of HSP70 gene by methidathion increased significantly on 4th days compared to control, but decreased significantly on 10th days.
The Effects of Moxidectin Nicotine-Conditioned Cue on Nicotine-Seeking Behavior in Mice
(inst Advanced Science Extension, 2021) Yunusoglu, Oruc; Demirkol, Muhammed Hamdi; Berkoz, Mehmet; Sagmanligil, Vedat; Oto, Gokhan; Ozdemir, Hulya
Current pharmacotherapies for nicotine abuse are few and relatively inefficient demonstrating the need for the development of new, effective remedies. Moxidectin is used as an anti-parasitic agent in both animals and humans, it also activates GABA receptors. The objective of the present investigation was to study the effect of moxidectin on nicotine-induced conditioned place preference (CPP) in male Swiss mice. Intraperitoneal (i.p.) route was used for nicotine (0.5mg/kg) administration for a 3-day conditioning program. The influences of moxidectin on the reinforcing characteristics of nicotine were tested in mice given i.p. treatment of moxidectin (5 and 10mg/kg) 30 minutes prior to per nicotine administration. CPP was extinguished by repeated testing, through which conditioned mice were daily given two doses of moxidectin (5 and 10mg/kg, i.p.). Subsequently, the potency of moxidectin in blocking the reinstatement of CPP provoked by priming given low-dose nicotine (0.1mg/kg, i.p.) was also evaluated. Moxidectin treatment illustrated a reserve of acquisition of nicotine-induced CPP. It was reduced priming nicotine-induced reinstatement and accelerated the extinction of CPP. Relatively nicotine enhanced the locomotor, motor activity but was not statistically significant. In conclusion, the outcomes demonstrate the potential for the development of moxidectin as a new pharmacotherapy for the treatment of nicotine addiction. (C) 2021 The Authors. Published by IASE.
The Health Risk Assessment of Essential Elemental Impurities (Cu, Mn and Zn) Through the Dermal Exposure of Herbal Ointment Extracted From Marjoram Herb (Majoranae Herbae Extractum)
(Springernature, 2022) Jurowski, Kamil; Folta, Maria; Tatar, Barbara; Berkoz, Mehmet; Krosniak, Miroslaw
Essential elements like Cu, Mn and Zn are extremely important for herbs' growth and physiological functions; however, from a toxicological point of view, the exposure of these elements (as essential elemental impurities) can exhibit potential harmful effects for patients. In Europe, very popular are ointments with Marjoram herb extract (Majoranae herbae extractum) as herbal medicinal products for adjunctively in rhinitis (runny nose). Based on posology of ointments with Marjoram herb extract, the exposure to these elemental impurities may be high during long-term use. Hence, the aim of this article is the health risk assessment of essential elemental impurities (Cu, Mn and Zn) through the dermal exposure of ointments with Marjoram herb extract (Majoranae herbae extractum) as herbal medicinal products applied adjunctively in rhinitis available in Polish pharmacies. The investigated essential elements were determined by well-validated methodology (R > 0.997, recoveries, LOD and LOQ values were acceptable) based on flame atomic absorption spectrometry (FAAS). Our results indicated that all analysed herbal medicinal products with Marjoram herb extract available in Polish pharmacies contain relatively low levels of essential element impurities, i.e. Cu (0.14-0.49 mg/kg), Mn (0.31-2.57 mg/kg) and Zn (0.73-3.19 mg/kg). The estimated exposure of the investigated elemental impurities confirms the safety of all products. To the best of our knowledge, the study about Cu, Mn and Zn contents in HMPs with Majoranae herbae extractum is described for the first time. The applied methodology and results are extremely important from regulatory toxicology point of view due to ICH Q3D elemental impurity guideline for pharmaceuticals.