Browsing by Author "Berktas, M."

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Anti-Tuberculous Drug Resistance of Mycobacterium Tuberculosis Strains Isolated From Van Region
(Elsevier Sci Ltd, 2008) Bozkurt, H.; Nalcacioglu, R. B.; Kurtoglu, M. G.; Kesli, R.; Berktas, M.
Antimicrobial Susceptibilities of Brucella Isolates From Various Clinical Speciemens
(Ivyspring International Publisher, 2011) Bayram, Y.; Korkoca, H.; Aypak, C.; Parlak, M.; Cikman, A.; Kilic, S.; Berktas, M.
Purpose: Brucellosis is a worldwide zoonotic disease and still constitutes a major public health problem. In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-Brucella agents and a possible new alternative tigecycline. Materials and Methods: A total of 56 Brucella isolates were enrolled the study and the identification of the isolates were based on conventional methods. In vitro activities of an-timicrobials were evaluated by the E test method. Results: All isolates were identified as B. melitensis. MIC90 values of doxycycline, strepto-mycin, rifampin, trimethoprim-sulfamethoxazole and tigecycline were 0.064 mg/L, 1 mg/L, 2 mg/L, 0.125 mg/L and 0.094 mg/L, respectively. Tigecycline had low MIC50 and MIC90 values against all B. melitensis strains; the highest MIC observed was 0.25 μg/mL. Conclusion: Our data suggest that tigecycline can be a therapeutic alternative option for the treatment of brucellosis. © Ivyspring International Publisher.
Bacillus Anthracis Sepsis in a Newborn
(Lippincott Williams and Wilkins, 2000) Özkaya, E.; Kirimi, E.; Berktas, M.; Odabaş, D.
Candida Albicans Outbreak Associated With Total Parenteral Nutrition in the Neonatal Unit
(Elsevier, 2016) Guducuoglu, H.; Gultepe, B.; Otlu, B.; Bektas, A.; Yildirim, O.; Tuncer, O.; Berktas, M.
Background: The most frequently isolated fungi in patients using TPN belongs to the Candida genus. Various infections including venous catheter infections, fungemia, endocarditis and ophthalmitis may be encountered. Objective: Upon growth of Candida in the blood cultures from the pediatric (neonatal) unit of our hospital, a surveillance was performed in this unit and involving the health care workers. Clonal relationships of the isolates were investigated with molecular tests. Methods: Blood samples obtained from the patients in pediatric neonatal unit were studied with automatized blood culture [BacT/Alert (Bio Merioux, France)]. Yeast isolates from environmental surveillance cultures (TPN solutions, hands of healthcare personnel, etagere, etc) and patients were identified as C. albicans with conventional methods and ID 32 C and ATB (TM) Fungus 3 (Biomerieux, France) kits. Clonal similarity was determined by using AP-PCR as initial method and we have also typified all strains by the method of REP-PCR (diversilab system, bioMerieux). Finally; Pulsed Field Gel Electrophoresis (PFGE) was used for confirmation. Results: C. albicans was isolated in blood cultures of seven patients. Similar antifungal susceptibility patterns were observed in all isolates. AP-PCR and REP-PCR showed that the C. albicans isolates grown in the TPN solution and from the patients' blood cultures were clonally same strains. PFGE analysis further confirmed this clonality. Conclusion: According to results of the molecular methods, we thought that a C. albicans outbreak had occurred in the neonatal pediatric unit, due to contamination of TPN solution.
Correlation of Slime Production Investigated Via Three Different Methods in Coagulase-Negative Staphylococci With Crystal Violet Reaction and Antimicrobial Resistance
(Sage Publications Ltd, 2009) Bozkurt, H.; Kurtoglu, M. G.; Bayram, Y.; Kesli, R.; Berktas, M.
This study investigated slime production by coagulase-negative staphylococci (CNS) using the standard tube (ST), Congo red agar (CRA) plate and Christensen's tube (CT) methods, and compared the results with those of the crystal violet reaction (CVR) test. The potential correlation between slime production and antimicrobial resistance was also evaluated. In total, 205 CNS strains were isolated from biological samples: 92 (44.9%) were shown to produce slime by the ST method; 96 (46.8%) by the CRA plate method; 90 (43.9%) by the CT method; and 89 (43.4%) strains were CVR positive. Eighty-three (40.5%) CNS strains were positive for slime production by the ST, CRA and CT methods. The findings of the ST, CRA and CT test methods were consistent with each other but were not related to CVR positivity. Based on the ST method, rates of antibiotic resistance to several antimicrobial agents were higher in slime-positive strains than in slime-negative strains and, in some cases, this was statistically significant.
Distribution of Hepatitis C Prevalence in Individuals According To Their Age Level in Eastern Turkey
(2008) Bozkurt, H.; Kurtoglu, M.G.; Bayram, Y.; Kesli, R.; Berktas, M.
Distribution, Optimum Detection Time and Antimicrobial Susceptibility Rates of the Microorganisms Isolated From Blood Cultures Over a 4-Year Time Period in a Turkish University Hospital and a Review of the International Literature
(Sage Publications Ltd, 2008) Kurtoglu, M. G.; Bozkurt, H.; Tuncer, O.; Kesli, R.; Berktas, M.
This study retrospectively examined 8986 blood cultures from patients over a 4-year time period in an eastern Turkish university hospital to determine the detection times and distribution of isolated microorganisms using the automated BACTEC (TM) 9050 and BACTEC (TM) 9120 systems. A total of 1914 (21.3%) blood cultures contained pathogenic microorganisms and 252 (2.8%) positive cultures were considered contaminated. Of all the cultures, 18 (0.2%) were false positives and 224 (2.5%) were false negatives. In cultures containing pathogenic microorganisms, Gram-positive and Gram-negative bacterial isolation rates were 436 (22.8%) and 1440 (75.2%), respectively, and yeasts (all Candida sp.) were found in 38 (2.0%) cultures. Coagulase-negative staphylococci occurred in 936 (48.9%) cultures and Staphylococcus aureus occurred in 302 (15.8%) cultures. The mean detection time for all of the pathogens was 21 h and Brucella spp were isolated within 10 days. This study helps in understanding the epidemiology of the region and in providing positive therapeutic approaches. A review of the international literature helps to place this understanding into a global context.
The Efficacy of Topical Ciprofloxacin in the Treatment of Chronic Suppurative Otitis Media
(Medquest Communications LLC, 1998) Kiris, M.; Berktas, M.; Egeli, E.; Kutluhan, A.
We conducted a test of the efficacy of ciprofloxacin eardrops in 80 patients (95 ears) with otorrhea due to chronic suppurative otitis media in two treatment settings. One group (n=40; 47 ears) received daily ciprofloxacin therapy plus aspiration in the clinic. The other group (n =40; 48 ears) self administered ciprofloxacin at home. Overall, otorrhea resolved in 88% of all ears within 12 days of the initiation of treatment. The clinic- treated patients tended to respond more rapidly than did the self-treated patients, but there was no statistically significant difference in success rates between the two groups. Side effects were negligible. We conch that empiric topical ciprofloxacin therapy is an effective, safe and relatively inexpensive treatment for otorrhea in patients with chronic otitis media.
Epidemiological Characteristics and Molecular Typing of Salmonella Enterica Serovar Typhi During a Waterborne Outbreak in Eastern Anatolia
(Maney Publishing, 2011) Bayram, Y.; Guducuoglu, H.; Otlu, B.; Aypak, C.; Gursoy, N. C.; Uluc, H.; Berktas, M.
In this study, we aimed to study the molecular and epidemiological characteristics of Salmonella enterica serovar Typhi (S. typhi) outbreak in Eastern Anatolia. Six hundred and thirty-seven patients from the same county with clinical diagnosis of typhoid fever were investigated with conventional methods from stool, urine and blood specimens. Antibiotic susceptibility tests and identifications were performed for positive specimens. Clonal relationships between the isolates were investigated using pulsed field gel electrophoresis (PFGE) method. A questionnaire was completed for the water consumption habits of patients. Of 91 culture positive specimens, 76 were blood, 13 were stool and 2 were urine. The isolates were resistant to ampicillin, ampicillin/sulbactam, chloramphenicol, cefuroxime, amikacin, gentamicin and trimethoprim-sulfamethoxazole. Although there was a single band difference in some isolates, PFGE results indicated that this was an outbreak caused by single strain according to the Tenover criteria. This outbreak thought to be associated with the consumption of tap water contaminated with sewage represents a breakdown of the basic public health and civil engineering infrastructure. Appropriate public health measures should be taken in order to avoid such outbreaks in the future.
The Evaluation of the Resistance Rates of Staphylococcus Aureus Strains To Antimicrobials According To the Years
(2007) Bozkurt, H.; Güzel Kurtoǧlu, M.; Bayram, Y.; Berktas, M.
In our hospital, sensitivity tests of 290 Staphylococcus aureus strains isolated in 1998-2000 years and 280 Staphylococcus aureus strains isolated in 2003-2004 years were performed and investigated the alterations on resistance rates. At two periods the resistance was not established to vancomycin; but, it was established an increase statistically unsignificant at the second period, amikacin, teicoplanin, gentamicin, tetracycline, oxacillin and eritromycin but increase in to ciprofloxacin and rifampicin significantly.
Hepatitis Delta Virus Infection in Van Region of Eastern Turkey
(1999) Turkdogan, M.K.; Akdeniz, H.; Berktas, M.; Hekim, H.