Browsing by Author "Beyhan, Y.E."

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A Case of Aural Myiasis Caused by Wohlfahrtia Magnifica in a Child in Turkey
(Refik Saydam National Public Health Agency (RSNPHA), 2017) Beyhan, Y.E.; Yilmaz, H.; Taş-Cengiz, Z.; Ayral, A.
Myiasis is the infestation of tissues and organs of vertebrate animals and humans by the larval stages of dipterous flies. In present case, a four year old child living in Silopi was applied to our clinic with complaints of otalgia, pruritus and otorrhea in the right ear. In the physical examination, aural fetor, secretion and several foreign bodies were observed. Twenty living maggots were removed from the external auditory canal (EAC) using surgical forceps. The maggots were identified as third phase larvae of Wohlfahrtia magnifica. In conclusion, patient should be examined for aural myiasis in case of otalgia, otorrhea, itching, and hearing impairments, especially in children.
A Comprehensive Retrospective Study: Intestinal Parasites in Human in Van Province
(Galenos Publishing House, 2019) Taş Cengiz, Z.; Yılmaz, H.; Beyhan, Y.E.; Çiçek, M.
Objective: The aim of present study was to retrospectively determine the prevalence of intestinal parasites in patients, who were referred to Parasitology Laboratory in Van Yüzüncü Yıl University, Faculty of Medicine during an 11-year period. Methods: Stool samples of 69633 individuals admitted to the outpatient clinics in the hospital were initially evaluated with native-Lugol, and then by flotation, sedimentation, trichrome staining, modified acid-fast staining and ELISA methods. Results: Twenty-four parasite species were identified in parasite-positive patients. At least one or more parasite species were found in 34.1% of all patients. The most commonly observed parasite was Blastocystis hominis (26.5%). Among pathogen parasites, Giardia intestinalis (G. intestinalis) was detected in 9.3%, Ascaris lumbricoides (A. lumbricoides) was detected in 2.5%, Entamoeba histolytica/Entamoeba dispar in 0.8%, Cystoisospora belli in 0.004%, Fasciola hepatica in 0.04%, Dicrocoelium dendriticum in 0.001%, Strongyloides stercoralis in 0.001% and hookworm in 0.001% of the patients. Conclusion: It was determined that, pathogen parasites such as G. intestinalis and A. lumbricoides were still observed at high rates in Van province, especially in children, and the problem of parasitosis is still continuing, although the prevalence of parasites has declined when compared to the previous years. © 2019 Turkish Society for Parasitology.
Cyclospora Cayetanensis, Opportunistic Protozoan Parasite, in Van Province, Turkey: a Report of Seven Cases
(2016) Taş Cengiz, Z.; Beyhan, Y.E.; Yılmaz, H.
The aim of this study is to report seven Cyclospora cayetanensis (C.cayetanensis) cases determined in Yuzuncu Yil University Medical Faculty Parasitology Laboratory, Van province, Turkey. In the study native-Lugol, formalin-ethyl acetate and modified acid-fast staining methods were performed to stool samples of the patients sent from outpatient clinics of the hospital. All of the patients infected with C. cayetanensis were older than 15 years. In our cases there were not a history of international travel. Only one of our seven cases was immunosuppressed. Trimethoprim-sulfamethoxazole (160/800 mg) was administered for the treatment of the patient. In conclusion, it was understood that the physicians should consider cyclosporiosis in the patients with diarrhea and abdominal pain.
Cystic Echinococcosis Seropositivity in the Blood Samples Sent To Parasitology Laboratory of Yüzüncü Yıl University Medical Faculty Between 2005 and 2013: Retrospective Assessment
(2015) Cengiz, Z.T.; Yılmaz, H.; Beyhan, Y.E.; Kotan M.Ç,; Çobanoğlu, U.; Ekici, A.; Ödemiş, N.
OBJECTIVE: This study was performed in order to contribute the data on the prevalence of cystic echinococcosis (CE) in Turkey and to reveal this parasitosis problem in Van province.; METHODS: Blood samples of 2642 patients (1214 men, 1428 women; 506 of them 0-13 age, 2136 of them 14 and over age group), which were sent with suspected CE to Parasitology Laboratory from various policlinics of Yüzüncü Yıl University Medical Faculty, between the dates of 01.09.2005 and 01.09.2013, were evaluated for CE by using Enzyme-Linked Immunosorbent Assay (ELISA) (R-Biopharm, Germany; IgG) method.; RESULTS: 801 (30.3%) of examined blood samples of these patients were found positive for this parasitosis. Seropositivity was found 31.9% of men, 29% of women; 33.4% of 0-13 age group, 29.6% of 14 and over age group in the study. Cysts, which were obtained from operated seropositive patients, were confirmed that unilocular cysts as pathological.; CONCLUSION: As a result, it was seen that CE is continued to be a major public health problem in Van province and it was appear that comprehensive protection and control programs should be carry out to struggle this disease.
Cystoisospora Sp. Infection Determined in Immunosuppressed and Immunocompetent Children: Three Cases Report
(2016) Taş Cengiz, Z.; Yılmaz, H.; Çiçek, M.; Beyhan, Y.E.; Dülger, A.C.
The aim of this study was to present three cystoisosporiasis cases diagnosed in pediatric patients of the Yuzuncu Yil University Medical Faculty. In the study, stool samples of the patients were evaluated by native-Lugol and modified acid-fast staining methods in the Parasitology Laboratory. The first case was a 4-year-old male child diagnosed with acute lymphoblastic leukemia (ALL). It was reported that the patient had abdominal pain, and permanent bloody and mucous diarrhea (8-10 times a day) was present for almost 1 week after the beginning of ALL treatment. The second case was a 10-year-old boy diagnosed with depression. The patient was brought to our hospital by his parents with complaints of abdominal pain, diarrhea, lack of appetite, weight loss, and fatigue persisting since 1 month in addition to headache, fear, sleeplessness, and waking up with cry. The third case was a 13-year-old boy who complained of abdominal pain, diarrhea (rare occasions), lack of appetite, and headache for 2 months. These patients had not traveled abroad. The cases were treated successfully with co-trimoxazole. Our results suggest that all patient groups with diarrhea and abdominal pain should also be considered in cystoisosporiasis.
Determination of the Prevalence of Opportunistic Intestinal Parasites in Children With Diarrhea
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) İşler, S.; Beyhan, Y.E.
The aim of this study is to determine the most frequent opportunistic intestinal parasites in children and demonstrate its importance. Study was conducted on patients between the ages 0 and 16. The study consisted of a total of 150 children, 66 girls and 84 boys. 44 of the girls and 56 of the boys (100 in total) had diarrhea. Evaluation was done by native-Lugol, flotation in saturated zinc sulfate, and modified acid-fast staining. Intestinal parasites were detected in 38% of 150 examined stool samples. Among them 41% of those with diarrhea and 32% of those without diarrhea were positive. The highest observed parasite species was detected as Blastocystis hominis (14.7%); and the lowest were Ascaris lumbricoides and Hymenolepis nana (0.7%). It was concluded that all children with gastro-intestinal complaints should be evaluated for intestinal parasites, mainly opportunistic ones, without preliminary screening whether the patient is diarrhea or not. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Evaluation of Cystic Echinococcosis Suspected Patients Applied To National Parasitology Reference Laboratory of Public Health Institution of Turkey Between 2009-2013
(2015) Beyhan, Y.E.; Babür, C.; Mungan, M.; Özkan, A.T.
OBJECTIVE: Cystic echinococcosis (CE) caused by the metacestode form of Echinococcus granulosus is an important public health problem common in our country. In this study, anti-E. granulosus antibodies were aimed to investigate in the serum samples of CE suspected patients who applied to the National Parasitology Reference Laboratory of Public Health Institution of Turkey.; METHODS: In the study, serum samples of 2921 patients which were sent to our laboratories from different hospitals between 1 January 2009 and 31 December 2013 were evaluated with at least one of the following tests: Enzyme-Linked Immunosorbent Assay (ELISA), Indirect Hemaglutination Assay (IHA) and Western Blot (WB) techniques.; RESULTS: Four hundred thirty nine (15.03%) of inspected 2921 samples were determined seropositive with at least one of the methods. When the results analyzed by gender, 13% of males and 16.40% of females were found positive. Examined the distribution of the results by years, with a maximum of 25% positivity was observed in 2009. Compatibility was determined at the rate of 91.4% among ELISA and IHA results; also 89.7% among WB and the other tests results.; CONCLUSION: Despite the gradual decreases the CE in Ankara and its surroundings, it is still continues to be a major public health problem. Essential prevention and control measures should be taken to reduce the prevalence of the disease. Also in the diagnosis of the disease, more reliable results can obtained with applying two tests (ELISA/IHA) together and confirm the positivity with WB.
Evaluation of Formalin-Fixed Paraffin-Embedded Tissue Samples Diagnosed by Histopathology as Echinococcus in Çorum
(Galenos Publishing House, 2021) Baş, Y.; Beyhan, Y.E.; Şahin, H.H.K.; Özçerezci, T.; Karasartova, D.; Güreser, A.S.; Özkan, A.T.
Objective: This study aimed to detect the presence of Echinococcus spp. in formalin-fixed paraffin-embedded (FFPG) samples of hydatid cyst cases and to discuss the DNA isolation problems in FFPG samples. Methods: FFPG samples of 47 cases diagnosed with hydatid cyst were included in this study. Demographic characteristics of the cases were investigated. Microtome sections were taken from the samples and deparaffinization, DNA extraction, polymerase chain reaction (PCR), and gel agarose electrophoresis procedures were performed. Results: Of the cases, 55.3% were female, whereas 45.7% were male. Average age was 45.47 and 68.1% of the cases were located in the liver, 17.0% in the lung, 12.8% in the abdomen, and 2.1% in the brain. DNA was obtained in only 11 (23.4%) of the FFPG cyst samples and no proliferation was detected in the PCR products of any of the sample. Conclusion: The scolex/germinal membrane’ absence in the FFPG sections, intense inflammatory cell reaction, presence of fibrosis and stromal/parenchymal tissue, DNA damage due to formaldehyde action, long-term archiving, and insufficient amount of DNA obtained were considered as factors preventing DNA replication in PCR. © 2021, Galenos Publishing House. All rights reserved.
The Importance of Antioxidant Enzymes and Oxidative Stress in Human Fascioliasis
(Galenos Publishing House, 2023) Cengiz, Z.T.; Yılmaz, H.; Beyhan, Y.E.; Ekici, A.; Çiçek, M.; Aydemir, S.
Objective: The aim of this study was to determine the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and malondialdehyde (MDA) in the patients infected with Fasciola hepatica and establish whether these parameters differ among the patients with fascioliasis. Methods: The patient group consisted of 140 individuals with F. hepatica seropositive; the control group consisted of 140 healthy individuals who tested negative for this parasite and had no other diseases. The patient group consisted of individuals with no chronic diseases other than fascioliasis; in both the patient and the control groups, the subjects had no unhealthy habits such as smoking and alcohol consumption, etc. The blood samples taken to diagnose fascioliasis were evaluated by the ELISA method. The samples were studied according to the kit procedures for SOD, CAT, GPx and MDA markers. Results: In this study, 43.6% of 140 individuals in the patient group infected with F. hepatica had CAT (p=0.001), 35% had GPx (p=0.001), 12.9% had SOD (p=0.002), 90.7% had MDA (p=0.001). There was found a statistically significant difference between the patient and the control group in terms of the positivity of these four parameters. Conclusion: As a result, a statistically significant relationship was found between the increase in the SOD, GPx, CAT, and MDA levels and fascioliasis. The high rate of MDA revealed that oxidative stress occurred in patients with fascioliasis, resulting in an increased activity of SOD, GPx, and CAT. © 2023, Galenos Publishing House. All rights reserved.
In-Vivo Comparison of the Efficacy of Nigella Sativa, Thymoquinone and Capsaicin Against Toxoplasma Gondii With Pyrimethamine-Sulfadiazine
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Yildiz, S.; Beyhan, Y.E.
Toxoplasma gondii (T. gondii) is estimated to infect a third of the world’s population although the majority of the infections are largely asymptomatic. The combination of pyrimethamine and sulfadiazine is considered the treatment of choice. These drugs cannot eliminate dormant tissue cysts. The aim of this study is to evaluate the therapeutic effects of Nigella sativa oil, thymoquinone and capsaicin. The study was carried out as two separate experiments. Balb/c mice were injected intraperitoneally with 105 and 103 T. gondii. After 24 hours, treatment was started with different doses of Nigella sativa oil, thymoquinone and capsaicin for 10 days. The effects of these components were evaluated compared to the combination of PYR-SDZ. Mortality and tachyzoite amounts of the mice were calculated for 30 days. In both experiments, the mice in the PYR-SDZ group lived to the fullest. Then the mice in the capsaicin group lived the longest. Since early losses occurred in both experimental groups, tachyzoite count was not performed in groups other than PYR-SDZ. Tachyzoites were not observed in the PYR-SDZ groups after day 10. It was determined that after the PYR-SDZ treatment of toxoplasmosis, capsaicin displayed the highest probability of survival. In conclusion, the combined use of capsaicin and PYR-SDZ can increase life expectancy by reducing the side effects of the drug. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of Cryptosporidium Spp. Antigen by Elisa in Stool Specimens: Nine Year Evaluation
(Galenos Publishing House, 2020) Beyhan, Y.E.; Yılmaz, H.
Objective: Cryptosporidium is an enteric protozoan parasite that affects human and many animal species in worldwide. Staining methods or stool antigen detecting methods are using for detection of Cryptosporidium in faeces. It is known that the ELISA method has high sensitivity and specificity in practice. The aim of this study is to demonstrate the frequency of this parasite by ELISA in samples sent to our laboratory between 2010 January and 2018 September. Methods: The study was conducted on a total of 723 patients, 431 men and 292 women, who were referred to the Parasitology Laboratory from various outpatient clinics due to digestive system complaints. The presence of Cryptosporidium spp. antigen was investigated by ELISA method. Results: Cryptosporidium spp. was not found in any patient with Nativ-lugol method, whereas Cryptosporidium spp. antigen positivity was detected in 2.8% of 723 patients. In the study, 2.5% of the males and 3.1% of the females were found positive in terms of having the parasite, and there was no significant difference in gender between the parasite frequencies. The highest rate of parasite positivity (4.5%) was found in the 0-6 age group. Conclusion: The high rate of cryptosporidiosis detected in our study is thought to be related to factors such as widespread animal husbandry in our region, poor hygiene rules and low socio-economic level. As a result of considering the findings of our study, evaluation of patients with intestinal complaints in terms of Cryptosporidium will be useful for accurate diagnosis, regardless of whether they have diarrhea and/or they are immunocompromised. © 2020 Turkish Society for Parasitology.
An Oral Myiasis Case Caused by Diptera (Calliphoridae) Larvae in Turkey
(Galenos Publishing House, 2019) Cengiz, Z.T.; Yılmaz, H.; Beyhan, Y.E.; Yakan, Ü.; Ekici, A.
The objective of the present study is to present a patient with oral myiasis caused by Calliphoridae larvae. The patient was a 63-year-old male who was transferred from Van Closed Prison to Yüzüncü Yıl University, Faculty of Medicine Research and Application Hospital. Five alive larvae localized in different points of gingival region were observed during routine oral cleaning. These larvae belonged to Calliphoridae family. As a result, medical staff should be informed about this infestation and this parasite should be kept in mind especially in risky group of patients and necessary controls should be conducted. © 2019 Turkish Society for Parasitology.
Prevalence of Entamoeba Spp. in Stool Samples of Patients With Amebiasis Suspect by Native-Lugol and Elisa
(2016) Beyhan, Y.E.; Yılmaz, H.; Taş Cengiz, Z.
OBJECTIVE: This study aimed to determine the prevalence of Entamoeba spp. in suspected stool samples submitted to our laboratory.; METHODS: In this retrospective study, stool samples of 998 patients with suspected amebiasis were sent from various clinics and services to our laboratory and were investigated by native-Lugol and enzyme-linked immunosorbent assay (ELISA) [for Entamoeba spp. antigen (Ridascreen® Entamoeba)] between January 2010 and December 2014.; RESULTS: By the end of the study, it was shown that 8.5% (85) of 997 patients, 7.45% (39) of males and 9.8% (46) of females whom amoeba antigen inspected in their stool samples, were positive. No parasite was identified by the saline-Lugol method. The highest antigen positivity was detected in the 25-44-year-old group with 11% positivity, and a high positivity of 23.2% was seen in March.; CONCLUSION: These results demonstrate that amebiasis is still a major health concern for our region. Although no parasite was detected during microscopic examinations, the detection of antigen positivity by ELISA reveals that microscopic examinations require experience and utilizing only microscopic examinations may lead to overlooks. To obtain more reliable results in diagnosis, ELISA analyses that use E. histolytica-specific monoclonal antibodies should be applied in addition to microscopic methods.
A Rare Case of Secondary Hydatid Cyst: Uterus and Colon Locations in the Same Patient
(Galenos Publishing House, 2019) Beyhan, Y.E.; Yılmaz, H.; Taş Cengiz, Z.; Yıldızhan, R.; Kotan, Ç.; Batur, A.; Halidi, A.G.
The aim of the present study was to present a case with secondary hydatid cysts in both uterus and colon. The patient was a 71-year-old female living in Hakkari, Turkey. She was admitted to the Van Yuzuncu Yil University Faculty of Medicine Medical Center with complaints of chronic abdominal and pelvic pain, and swelling in the abdomen. First, the sagittal T2 weighted magnetic rezonance imaging (MR) showed a type-3 cyst hydatid with daughter vesicles located at the posterior of uterus. Later, MR revealed a type-2 cystic lesion with detached membrane adhered to the anterior wall of colon and it was reported to be associated with abdomen. When the previous liver surgery history of the patient was kept in mind, the new finding was suggestive of a secondary cystic hydatid . In conclusion, it is possible to diagnose secondary cystic echinococcosis in patients with a history of primary cyst surgery in liver or any other organ by combining the symptoms and imaging findings. ©Copyright 2019 Turkish Society for Parasitology-Available online at www.turkiyeparazitolderg.org.
Seropositivity of Toxoplasma Gondii Among Blood Donors and Patients With Hematologic Malignity
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Yıldız, S.; Esen, R.; Karaman, K.; Beyhan, Y.E.
Toxoplasma gondii (T. gondii) is an opportunistic parasite. Transmission from T. gondii-infected donors to recipients is possible through blood and organ transplantation. In this study, it was aimed to investigate the presen ce of T. gondii antibodies in healthy donors and in blood recipients with immunosuppressed hematological malignancies. A total of 92 patients included in the present study (46 donors and 46 patients). Sixteen of the hematological patients had lymphoblastic leukemia and 14 had acute myeloblastic leukemia. The presence of IgG and IgM Toxoplasma antibodies and IgG avidity values were investigated by ELISA. Twenty-two (47.8%) of patients and 11 (23.9%) of the donors were found to be positive for T.gondii IgG antibodies. IgG positivity was detected in all patient groups except those with aplastic anemia. The highest IgG positivity was found in acute myeloblastic leukemia (8) and lymphoblastic leukemia (5). IgM seropositivity was detected totally three cases (3.3%), two of 46 patients (4.3%) and one 46 of donors (2.2%). Although the number was low, all patients with chronic myeloid leukemia were detected both IgG and IgM positive (100%). Low avidity was found in all three of the IgM positive cases. Considering that toxoplasmosis is an infection that causes severe clinical symptoms and death, routine diagnostic methods that detect the presence of parasites in donor and patient groups will be beneficial for the prevention of T.gondii transmission. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Seroprevalance of Leishmaniasis in Dogs From Hatay and Burdur Provinces of Turkey and Northern Cyprus
(2016) Beyhan, Y.E.; Çelebi, B.; Ergene, O.; Mungan, M.
OBJECTIVE: This study aimed to investigate the seroprevalance of leishmaniasis in dogs from Hatay and Burdur provinces of Turkey and Northern Cyprus.; METHODS: Blood was collected from a total of 278 dogs, including 124 from Hatay, 49 from Burdur, and 105 from Northern Cyprus. Dilutions of serum samples were prepared, and the presence of anti-Leishmania antibodies was investigated by indirect fluorescent antibody technique (IFAT).; RESULTS: A total of three dogs were found to be seropositive (1.1%), one from Hatay (0.8%) and two from Northern Cyprus (1.9%). Also, one dog (0.4%) from Northern Cyprus was found to be borderline positive. All dogs from Burdur have been identified as seronegative.; CONCLUSION: This is the first research on the seroprevalence of the parasite in dogs from Hatay and Burdur. The seropositivity detected in dogs from Hatay and Northern Cyprus demonstrates the presence of the parasite in these regions, and obtained results contribute data on the prevalence of the disease in an epidemiological manner. To obtain more reliable data, it will be useful to conduct studies on wider dog populations and vector sandflies.
The Species of Ticks Bites on Human and Their Seasonal Distribution in Ankara, Turkey
(Chartered Inst. of Building Services Engineers, 2016) Beyhan, Y.E.; Mungan, M.; Babur, C.
Ticks (Acari: Ixodoidea) are among the most significant blood-sucking arthropods worldwide. In humans, they can cause severe toxic conditions such as paralyses, irritation and allergy. Besides these, they transmit a great variety of infectious diseases caused by viral, bacterial and protozoal agents, including Crimean-Congo hemorrhagic fever (CCHF) virus. This study was performed for evaluation of received 1562 samples from humans with the complaint of tick bite to National Parasitology Reference Laboratories between January 2011 and December 2013. Ticks were taken directly into 70% alcohol and have been identified under the stereo microscope. It was found that 1497 (95.84%) were ticks, 39 were (2.50%) artifacts such as scab and blood clots, 22 (1.41%) were different arthropods of non-medical importance, 3 were (0.19%) pubic lice (Pthirus pubis), 1 was (0.06%) bedbug (Cimex lectularius). The ticks were identified as follows: 322 Rhipicephalus sangiuneus group, 241 Haemaphysalis parva, 188 Hyalomma marginatum, 175 Hyalomma spp. nymph, 138 Rhipicephalus spp. nymph, 109 Rhipicephalus bursa, 79 Ixodes ricinus, 68 Dermacentor marginatus, 59 H. anatolicum, 52 H. detritum, 17 H. excavatum, 9 Haemaphysalis punctata, 8 Ixodes spp. nymph, 1 Haemaphysalis spp. larva, 1 Haemaphysalis spp. nymph and 1 Argas spp. 29 ticks could not be identified, since they had morphological abnormalities or deficiencies. During January to December, tick bites were reported in all of the months. Most cases were occurred between April and September; 365 (24.38%) cases in June and 298 (19.91%) in July. In recent years, people have become more susceptible to infestations with ticks and other ectoparasites. So, it is concluded that informing the public about ticks, tick borne diseases and teaching them the ways to protect would be useful. © 2016, Chartered Inst. of Building Services Engineers. All rights reserved.
Urogenital Myiasis Caused by Psychoda Albipennis (Diptera: Psychodidae) in a Woman in Siirt
(2015) Beyhan, Y.E.; Yılmaz, H.; Baran A.İ,; Taş Cengiz, Z.; Yakan Ü,; Ekici, A.
Urogenital myiasis cases occurring with the settlement of larvae of flies belonging to the order Diptera are facultative and are rarely encountered in humans. In this study, urogenital myiasis caused by Psychoda albipennis in a 20-year-old female patient was presented. The patient was admitted to our hospital with complaints of nausea, vomiting, and dysuria and claimed that she saw motile larvae in her urine. Five larvae collected from the patient's urine were microscopically examined, and they were identified as fourth-stage larvae of Psychoda albipennis. Complaints of the patient ceased after the application of an antibiotic and urinary antiseptic. It was concluded that myiasis should be considered in patients with urogenital complaints.