Browsing by Author "Bora, Gulhan"

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The Effects of Asphodel (Asphodelus Aestivus Brot.) Rhizome Extract Administration in the Treatment of Thermally Induced Wound in Rats
(Assoc Pharmaceutical Teachers india, 2021) Arihan, Okan; Akgul, Omer; Oto, Gokhan; Yildirim, Serkan; Eroglu, Huseyin; Bora, Gulhan
Background: Burn wound is an important health problem worldwide. Serious outcomes related with burn occur and treatments focus on healing of the wounded area with dermal preparations containing chemicals avoiding growth of pathogenic organisms. Plants are important sources for both modern pharmacy to find new molecules used in modern medication as well as in traditional medicinal practices. Many plants are used for treatment of burn wounds. Asphodel (Asphodelus aestivus Brot.) is a naturally found plant in the Mediterranean flora. It is also used in traditional medicinal practices in Turkey. Aim: In this study it was aimed to test wound healing effect of Asphodel. Materials and Methods: An ointment prepared with a 5% methanolic extract of Asphodel was used. 4 groups were formed as control, wound, wound+asphodel and wound+standard drug (oxytetracyclin hydrochloride) (n = 8 in each group). Burn wound was established with a thermal induction. Biochemical, microbiological and histopathological evaluations were performed. Results: Biochemical results (Alt, Ast, Glucose, Ldh) obtained from blood samples showed no adverse effect of this administration. Microbiological results showed a protective effect of Asphodel similar with standard drug. Histopathological evaluation also presented an ameliorating effect as standard drug. Conclusion: Results of this study states that traditional use of this plant for the treatment of burn/wound/ulcer in traditional medicine applications is valid.
Investigation of the Gene Carriage Rates for Staphylococcus Aureus, Meca, Vana and Nuc Genes in the Nasal and Milk Specimens From the Sheep Caretakers With Sheep
(Sivar-soc Italiana veterinari Animali Reddito, 2021) Akgul, Omer; Bora, Gulhan; Guducuoglu, Huseyin
YMethicillin-resistant S. aureus (MRSA) is an important pathogen that may cause serious infections in the humans and animals. The pathogenity of Staphylococcus aureus emerges associated with the factors such as antibiotic resistance, immune evasion, invasion capability and virulence. By the impairment of the immune system, S. aureus-borne skin and soft tissue infections as well as serious infections such as pneumonia, septicemia and osteomyelitis may develop in the human bodies. S. aureus is one of the most common causes of the intramammary infections (IMI) in the dairy ruminants. The present study aimed to identify the presence of S. aureus, mecA and vanA genes in humans and animals (sheep) in the rural corporations on commercial dairy sheep farms in Eastern Turkey. It was also targeted to evaluate nuc gene positivity of Stapyhylococcus aureus strains isolated from humans and animals. Totally 78 (12.7%) S. aureus strains were isolated and identified from 612 materials taken from the sheep caretakers (nasal swab: 204) and the sheep (204 specimens for nasal swab and 204 specimens for milk each from sheep). S. aureus was distributed in 27 (13.2%; 204), 16 (7.8%; 204) and 35 (17.2%; 204) of the nasal swab specimens taken from the sheep caretakers, the sheep and sheep's milk specimens, respectively. Antibiotic susceptibility testing of 78 S. aureus isolates performed by Vitek2 device revealed that the highest antibiotic resistance was against benzylpenicillin. Gene analysis for 12 MRSA strains isolated in the specimens of the sheep caretakers and sheep was performed by single-Polymerase Chain Reactions (sPCR) for detection of mecA and vanA genes. Twelve MRSA isolates were found positive for mecA gene carriage. On the other hand, 78 S. aureus isolates were not found to carry vanA gene. All of the 12 MRSA and 66 Methicilline-susceptible S. aureus (MSSA) isolates were found positive for nuc gene carriage. It was concluded that MRSA strains isolated from the sheep caretakers and sheep had impact on the public health and created at risk for food chain.
A Neonatal Septic Arthritis Case Caused by Klebsiella Pneumoniae: A Case Report
(Premchand Shantidevi Research Foundation, 2016) Ozsari, Tamer; Bora, Gulhan; Ozdemir, Ozmert M. A.; Kilic, Ilknur
Septic arthritis is encountered very rarely during the neonatal period and its diagnosis can delay because of atypical symptoms, thus it may lead to serious sequelae. The sequale can be prevented by early diagnosis and concomitant treatment. In neonates, pain can be experienced as a result of pseudoparalysis and of movement of the effected joints. A 17-day-old neonatal patient was brought to our hospital with complaint of unrest and then diagnosed with septic arthritis due to propagation of Klebsiella pneumoniae in joint fluid culture was represented because of the rarity of such a case.
The Prevalence of Rotavirus and Adenovirus in the Childhood Gastroenteritis
(Kowsar Publ, 2016) Ozsari, Tamer; Bora, Gulhan; Kaya, Bulent; Yakut, Kahraman
Background: Acute gastroenteritis stemming from viral causes is very common during the childhood period. Rotavirus and enteric adenovirus are the most common factors of acute gastroenteritis encountered in infants and children. However, the epidemiology of rotavirus and enteric adenovirus gastroenteritis in the east Anatolia region is not well-known. Objectives: We aimed to evaluate the relationship between the distribution of antigen positivity in rotavirus and enteric adenovirus antigen tests required cases and demographic data retrospectively in pediatric patients admitted to our hospital. Patients and Methods: The records of stool sample analyses for 1154 patients admitted to our hospital from June 2011 to December 2011 with complaints of diarrhea were retrospectively examined. The presence of rotavirus and enteric adenovirus antigens in stool specimens was investigated by means of an immunochromatographic test. Results: Viral antigens were detected in 327 (28.3%) stool specimens out of 1154. Among the positive results, the frequency was 73.7% for rotavirus and 26.2% for adenovirus. While the detected rotavirus antigen rate was high for all age groups, it was highest for children under the age of 2, with a rate of 57.1%. Moreover, the rotavirus infections were observed at a rate of 44.3% in winter and of 24.6% in autumn. Conclusions: The most important factor in childhood acute gastroenteritis in east Anatolia is the rotavirus. Rotavirus and adenovirus antigens should be routinely investigated as a factor in fresh stool samples for the accurate diagnosis and treatment of gastroenteritis in children in the winter and autumn months.