Browsing by Author "Boynukara, B"

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Avipoxvirus Infection in Quails
(Tubitak Scientific & Technological Research Council Turkey, 2005) Gülbahar, MY; Çabalar, M; Boynukara, B
The present study describes clinical, virological, histopathological, immunohistochemical and electron microscopic findings of pox infection encountered in a quail flock. Lesions consisted of single or multiple nodules with a crust in variable size of gray to yellow or dark brown discoloration on the comb, eyelids, and the other poorly feathered areas of the body. Histopathological changes consisted of hyperplastic epithelium overlying, with ballooning degeneration of keratinocytes, many of which had eosinophilic intracytoplasmic inclusion bodies (Bollinger bodies), and heterophilic infiltrations admixed with mononuclear cells extending into dermis. Avipoxvirus infection was confirmed by positive immunostaining in the cytoplasm of affected cells for poxvirus antigen, excluded no immunostaining of inclusion bodies, using streptavidin-biotin peroxidase complex method and by demonstration of brick-shaped virus particles with a central core using direct electron microscopy. Typical thickness of chorio-allantoic membranes (CAMs) infected with virus, compared with controls, was observed, and the harvested virus on CAMs was detected as positive against known fowlpox virus using gel diffusion test.
Brucellosis in Horses Around Van, Turkey
(indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Aksakal, A; Gulhan, T; Boynukara, B
A programme is being carried out in Turkey to eradicate brucellosis from cattle by compulsory slaughtering of positive cases on serological test surveillance. In spite of the very strict application of the programme, the results obtained so far are not satisfactory in respect of eliminating the disease. It seems that it will be very difficult to achieve this goal without considering the disease status in other domestic animals. Brucella abortus is associated with chronic inflammatory conditions including fistulous withers, poll-evil and also joint infection in horses. Consequently, in this study, to evaluate prevalence of brucellosis in horses, a comparative study of standard tube agglutination test (STAT) and Rose Bengal Plate Agglutination Test (RBPT) were carried out on horse sera.
The Characterization of Protein Profiles of the Aeromonas Hydrophila and A-Caviae Strains Isolated From Gull and Rainbow Trout Feces by Sds-Page
(Scientific Technical Research Council Turkey, 2003) Körkoca, H; Boynukara, B
Ten motile Aeromonads, including one Aeromonas hydrophila ATCC 7512, were used. Of the strains, five were isolated from gulls (one A. hydrophila strain and four A. caviae strains) and four from rainbow trout (three A. hydrophila strains and one A. caviae strain). Whole cell protein profiles of these strains were analyzed by SIDS-PAGE. Forty-five pairs were formed in comparing the protein profiles of each strain. Of the pairs, 17 (37.8%) were different, 18 (40%) were unclear and 10 (22.2%) were identical. When the strains belonging to the same species were compared within themselves, four A. caviae strains were found to be identical, while all four A. hydrophila strains were different or of uncertain relationship to the group. One A. hydrophila strain isolated from gulls was found to be identical to four A. caviae strains again isolated from gulls. In this study, A. hydrophila and A. caviae strains were isolated from gull feces and their protein profiles were shown by SDS-PAGE for the first time in Turkey. These findings are expected to contribute to future studies in this field.
The Characterization of Protein Profiles of the Isolated Aeromonas Sobria Strains From Animal Faeces by Sds-Page
(indian veterinary Journal, 2004) Boynukara, B; Korkoca, H; Senler, NG; Gulhan, T; Atalan, E
Aeromonas spp. are members of the Vibrionaceae (Janda, 1991). Although they are common in fresh surface water, their presence in faeces of several animals and humans has been proved (Boynukara et al., 2002; Jindal et al., 1993). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins have been used for typing several bacterial species, including Aeromonads (Stephenson and Tabaqchali 1986; Stephenson et al., 1987). A more sensible approach might be to identify isolates as A. hydrophila, A. sobria or A. caviae, and use an electrophoretic fingerprinting technique to further characterize them (Popoff, 1984), Maclnnes et al. (1979) did the first DNA hybridization experiments with Aeromonas, and concluded that the genus consist of two main evolutionary lines; a diverse group of motile Aeromonads and genetically more homogeneous non-motile Aeromonads. Fanning et al. (1985) confirmed that all strains of Aeromonas were more closely related to each other than to species in other families, and found that motile Aeromonas species could be divided into at least 10 different DNA hybridization. In this study, we aimed to characterize the Aeromonas sobria isolated from Turkish Van cat, chicken, gull and Rainbow trout and identified according to Popoff (1984) using SDS-PAGE method.
Investigation on Carriage of Clostridium Difficile in Van Cats
(indian veterinary Journal, 2003) Solmaz, H; Aksakal, A; Tutuncu, M; Akkan, HA; Boynukara, B
Isolation of Motile Aeromonads From Turkish Van Cats
(indian veterinary Journal, 2002) Boynukara, B; Gülhan, T; Boynukara, Z
Prevalence and Characteristics of Campylobacter Species Isolated From Gallbladder of Slaughtered Sheep in Van, (Eastern) Turkey
(veterinarni A Farmaceuticka Univerzita Brno, 2006) Ekin, IH; Gürtürk, K; Arslan, A; Boynukara, B
To determine the prevalence of campylobacter species in gallbladder of sheep in Van, ( Eastern) Turkey, a total of 220 gallbladder samples from healthy slaughtered sheep were examined bacteriologically in October 2000 and 2002. Of the 110 samples examined each year, 27 ( 24.6%) and 24 ( 21.8%) campylobacter strains were isolated, respectively. Of the 27 campylobacter strains isolated in the year 2000, 14 ( 51.9%) were identified as C. jejuni, 7 ( 25.9%) C. fetus, 3 ( 11.1%) C. coli and 3 ( 11.1%) C. lari. Similar results were obtained in the study performed in 2002, but C. lari could not be isolated. Growth and biochemical characteristics of all identified Campylobacter species with some exceptions were typical of each species. Six of 13 examined C. fetus strains grew well at both 25 degrees C and 42 degrees C in thioglycollate medium and on blood agar. C. jejuni strains differed from C. coli only by Na-hippurate hydrolysis test. Results of the present study revealed that C. jejuni is the most common campylobacter species isolated from gallbladders of sheep. The thermophilic campylobacters in significant proportions may cause contamination of carcass during slaughter and transmission of the food-borne pathogens to humans.
Prevalence of Rotavirus, Escherichia Coli K99 and O157:h7 in Healthy Dairy Cattle Herds in Van, Turkey
(Tubitak Scientific & Technological Research Council Turkey, 2001) Çabalar, M; Boynukara, B; Gülhan, T; Ekin, IH
A survey was conducted to determine the prevalence of rotavirus, Escherichia coli K99 and O157:H7 in healthy dairy cattle herds in Van and environs. Turkey. Some of the cattle herds had a history of recurrent neonatal diarrhoea for previous years, and even while sampling was being done, some newborn calves were diarrhoeic. Three hundred and twelve faecal specimens from dairy cattle farms free of clinically apparent diarrhoea were tested both for the presence of rotaviruses by PAGE silver staining and for Escherichia coli K99 and O157:H7 by latex agglutination using specific antisera after the growth of each colony. The enteropathogenic characteristic of K99 and O157:H7 isolates was determined by rabbit ileal loop test. No rotavirus was detected from clinically normal cows, heifers or calves. Only 1 of 9 diarrhoeic calves was found to be positive for rotavirus. Out of the 235 E, coli isolates from non-diarrhoeic animals, 28 were positive for K99 and 4 for O157:H7. While 14 of 28 K99 isolates were found to be positive for enteropathogenic Escherichia coli (EPEC), 2 of 4 O157:H7 isolates were also positive for EPEC. An in vitro antibiotic sensitivity test of K99 and O157:H7 isolates indicated that the most sensitive antibiotics were enrofloxacin and danofloxacin.
Seroprevalence of Brucellosis in Stray Dogs in the Region of Van, Turkey
(indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Boynukara, B; Aksakal, A