Browsing by Author "Bozkurt, Hamza"

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Evaluation of Hbv-Dna in Patients With Different Serologic Markers
(Galenos Yayincilik, 2005) Guducuoglu, Huseyin; Bozkurt, Hamza; Yaman, Gorkem; Kutluay, Nihat; Berktas, Mustafa
In this study, patients infected with HBV with uncharacteristic serologic markers were interpreted and their probable clinical stages were determined. With this purpose, 75 patients were evaluated for HBV-DNA and hepatitis serologic markers. HBV-DNA results were obtained using bDNA-Bayer kits and serologic markers were detected using microparticle enzyme immunoassay (MEIA) kits. From 75 patients, 54 (72%) were HBV-DNA positive, and 35 (64.8%) of them were HBeAg positive. From 21 (28%) patients who are HBV-DNA negative, only 1 (4.8%) patient was HBeAg positive. HBsAg, HBeAg, anti-HBe, anti-HBc IgM and anti-HBc IgG of 46 patients were compared with HBV-DNA results simultaneously and 31 (67.4%) patients were HBV-DNA positive and HBsAg, HBeAg, anti-HBe, anti-HBc IgM, anti-HBc IgG results were positive in 29 (63%), 22 (48%), 7 (15%), 1 (2%), and 29 (63%) patients respectively. In 15 (33%) patients with negative HBV-DNA results, HBsAg in 14 (30%), HBeAg in 1 (2%), anti-HBe in 13 (28%), anti-HBc IgG in 14 (30%) were positive, and none of them were positive for anti-HBc IgM.
Evaluation of Western Blot Method for the Detection of Antibodies To Helicobacter Pylori Antigens in Patients With Gastric Carcinoma and Cases With Epigastric Complaints
(Ankara Microbiology Soc, 2010) Guducuoglu, Huseyin; Berktas, Mustafa; Bozkurt, Hamza; Ozer, Turkan Toka; Bulut, Guelay; Ozturk, Oznur; Ilhan, Mahmut
Helicobacter pylori proteins CagA (cytotoxin-associated gene A) and VacA (vacuolating cytotoxin A) are among the virulence factors of this species. CagA gene carrying H.pylori strains are particularly associated with gastric adenocarsinoma. This study was conducted to evaluate Western Blot (WB) method to determine specific H.pylori antibodies in a group of patients with gastric cancer and in a control group with no malignancy. A total of 99 patients with gastric cancer (94 adenocarcinoma, 2 adenosquamous cell carcinoma, 3 non-Hodgkin lymphoma) and 150 control cases with epigastric complaints such as nausea, vomiting, diarrhea, gastroesophageal reflux and abdominal pain, were included to the study. H.pylori IgG-ELISA was positive in all study (mean age: 56.7 +/- 1.2 years, 62 male) and control (mean age: 24.2 +/- 1.3 years, 64 male) patients. Specific antibodies against CagA, VacA, OMP (outer membrane protein)-67, urease-A, urease-B, HSP (heat shock protein) and flagellin antigens determined by a commercial WB-based kit (RIDA Blot Helicobacter, R-Biopham GmbH, Germany). Interestingly, no anti-VacA positivity was detected in none of the patient and control groups. The positivity rates for H.pylori CagA, OMP-67, urease A, urease-B, flagellin and HSP specific antibodies were as 78%, 54%, 37%, 60%, 53% and 82% in the gastric cancer group and 85%, 71%, 55%, 43%, 61% and 75% in the control group, respectively. There was no statistically significant difference (p> 0.05) between gastric carcinoma and control groups in terms of CagA, HSP and flagellin antibodies (p> 0.05). On the other hand, a statistically significant difference was found between the 2 groups in terms of urease-A, urease-B and OMP-67 (p< 0.01). These results suggested that this test should be assessed again by the manufacturer for its detection power directed towards specific H.pylori antibodies, especially for Vac-A. Further molecular and clinical studies are necessary to determine the factors that affect H.pylori virulence and disease prognosis.
Hepatitis D Virus Seroprevalence Determined During Periods of Hepatitis B Virus Infections in Eastern Turkey
(Galenos Yayincilik, 2009) Guzel Kurtoglu, Muhammed; Ustun, Cerna; Bozkurt, Hamza; Tuncer, Oguz; Berktas, Mustafa
A serological marker of Hepatitis D virus (HDV), anti-HDV JgG was investigated in the sera of 955 patients with Hepatititis B virus (HBV), who were admitted to Van Yuzuncu Yil University Medical School Hospital and Van Yuksek Ihtisas Education and Research Hospital in Van between January 2004 and December 2005. In this prospective study, markers of HBV serology were investigated in Axsym System device via macro-ELISA method, but anti-HDV JgG was investigated via HDV JgG Qua/it kits using micro-ELISA method. HBV DNA tests were conducted using Bayer Versant HBV DNA3.0 Assay reactives and Quantiplex TM System b-DNAAnalyzer device. A total of, 955 HBV-infected patients between 6 and 97 years of age were included into the study. Of the patients, 35.3% had acute HBV infections, 54.6% had chronic HBV-infections and 10.2% were inactive HBsAg carriers. Of 955 HBV-infected patients, 5.8% were anti-HDV lgG positive. Of HDV seropositive patients, 43.6% had acute HBV infections, 52.7% had chronic HBV infections, and 3.6% were inactive HBsAg carriers. HDV seroprevalence rate found in the study was determined to be lower than that among general population in Turkey. Since HBV/eads to HDV in only HBV-infected individuals,. HDV should be investigated in HBV-infected patients.
Identifcation of Dermatophytes and Isolation of Dermatophytes in the Van Region
(1997) Bozkurt, Hamza; Berktaş, Mustafa
Çalışma, yüzeysel mantar enfeksiyonu olduğu düşünülen 1074 hastadan alınan örnekler üzerinde yapılmıştır. Bu örneklerin 342'si (%31.84) ayak, 237'si (%22.07) el, 208'i (%19.37) saçlı deri, 197'si (%18.34) gövde, 47'si (%4.38) kasık ve 43'ü (%4.00) tırnak bölgesinden alınmıştır. Alınan tüm örnekler önce direkt mikroskopik olarak incelenmiş, daha sonra etkenlerin izolasyonu amacıyla ikişer adet Sabouraud Dextrose Agar (SDA), Potato Dextrose Agar (PDA) Mycobiotic Agar (MBA) ve Dermatophyte Selective Agar (DSA) besiyerlerine ekilereki ekimlerden birisi 22-26C oda ısısında, diğeri 37C 'ye ayarlanmış etüvde inkübe edilmiştir. İzole edilen dermatofitler önce üreme hızı, yüzey görünümü, yüzey örgüsü, yüzey pigmenti, koloni tabanında pigment oluşumu, oda ısısında yada 37C'de üreyip ürememe özellikleri gibi makroslopik özellikleri açısından incenlenmiş, daha sonra selofan bant yöntemi ile Laktofenol Pamuk Mavisi preparasyon hazırlanarak dermatofitlerin hif ve spor yapıları gibi mikroskopik özellikleri incelenerek kaydedilmiştir. Ayrıca identifikasyonda üreaz oluşturma ve kıl delme deneyi gibi yöntemlerden de yararlanılmıştır. 1074 Örneğin 215 (%20.02)'inde direkt mikroskopi pozitifliği, 221 (%20.58)'inde ise kültür pozitifliği saptanmıştır.Alınan örneklerden 179'unda dermatofit,42'sinde Candida olmak üzere toplam 221 örnekten etken izole edilmiştir. Kültürden izole edilen 179 dermatofitin dağılımında;93 (%51.96)'ü T. rubrum, 51 (%28.49)'i T mentagrophytes, 13 (%7.26)'ü T. tonsurans ve 2 (%1.15)'si T. verrucosum olarak saptanmıştır. Bu dermatofitlerin izole edildikleri vücut bölgelerine göre dağılımında ise; ayak ve tırnakta en sık T. rubrum'un, gövde ve kasıkta en sık T. mentagrophytes'in, saçlı direde T. violacem'un en sık etkenler oldukları görülmüş, el bölgesinde ise T. rubrum ve T. mentagrophytes'in aynı oranlarda etken oldukları saptanmıştır.
Investigation of Borrelia Burgdorferi Seroprevalence in Van Region of Turkey
(Galenos Publ House, 2008) Bozkurt, Hamza; Ciftci, Ihsan Hakki; Guducuoglu, Huseyin; Berktas, Mustafa; Korkoca, Han F.; Akdeniz, Hayrettin
A total of 460 sera (226 from women and 234 from men) were obtained randomizedly in the region of Van. In the sera samples, the antibody of B. burgdorferi was investigated. For the purpose of distinguishing between B. burgdorferi antigens and the other diseases with similar antigenic characteristics, rheumatoid factor and Treponema pallidum antibody assays were performed. At the end of the study, total seropositivity was found as 6.3%, being 5.75% in women and 6.84% in men. Besides, the rate of seropositivity in the rural areas of Van, especially Ozalp (22.58%), Caldiran (18.75%) and Baskale (10%) was found higher. In conclusion, Lyme borreliosis can not be ignored in the region of Van, especially in rural areas and preventive medicine services should be aware of this disease.
The Relationship of Hepatitis C Seroprevalence and Age, Sex, and Hemodialysis Time of Patients Undergoing Hemodialysis
(Galenos Yayincilik, 2006) Kurtoglu, Muhammet Guzel; Bozkurt, Hamza; Kesli, Recep; Berktas, Mustafa
In the present study which investigates the effect of hemodialysis time to hepatit C virus (HCV) seroprevalence, sera belonging to 216 patients treated with hemodialysis from three centers were used as materials. Microparticle Enzyme Immune Assay (MEIA) and chemiluminescence assays were used to investigate anti-HCV antibodies in patients sera and the obtained results were recorded. The relationship among sex, age and hemodialysis time of the patients and the obtained results were investigated. It was established that anti-HCV positivity is 23.2% in men and 14.4% in women, and mean HCV seroprevalence is 19%. The mean age of the patient is 47.8 +/- 17.3 year, mean dialysis time is 47.8 +/- 42.5 month. In conclusion, while there is not any relationship among anti-HCV positivity, sex and the age of patients, there was statistically important relationship between anti-HCV positivity and beginning time to dialysis (r=0.501, p=0.0001).
Sperm Motility Changes After Coincubation With Various Uropathogenic Microorganisms: an in Vitro Experimental Study
(Springer, 2008) Berktas, Mustafa; Aydin, Sabahattin; Yilmaz, Yuksel; Cecen, Kursat; Bozkurt, Hamza
Objective We investigated the relationship between semen motility and various uropathogenic microorganisms. Materials and methods Semen specimens from healthy donors were divided into portions and incubated with uropathogenic microorganisms in concentrations varying from 2 x 10(3) to 2 x 10(7) microorganisms/ml(-1). Uninfected suspensions of spermatozoa served as controls. In all samples, sperm motility was examined at the second, fourth, and sixth hours after incubation in order to assess motility as a function of time. Enterobacter aerogenes, Staphylococcus epidermidis, S. aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were used as bacterial agents. Besides these bacterial strains, Candida albicans was also used. Results and conclusions Observed negative impact on sperm motility was not correlated with microorganism concentration. However, until a certain concentration threshold, this impact was prominent. Regardless of the microorganism, this deleterious effect could not be confirmed in specimens coincubated with lower microorganism concentration. No or poor correlation was found between motility and bacteria concentration except with E. aerogenes at the second hour. The data indicates that sperm function impairment is not related to direct sperm and bacteria interaction. Instead, bacterial concentration enough to change the environment or to consume high energy might result in motility loss.