Browsing by Author "Can, Ismail"

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Royal Jelly Modulates Oxidative Stress and Apoptosis in Liver and Kidneys of Rats Treated With Cisplatin
(Hindawi Ltd, 2011) Karadeniz, Ali; Simsek, Nejdet; Karakus, Emre; Yildirim, Serap; Kara, Adem; Can, Ismail; Turkeli, Mehmet
Cisplatin (CDDP) is one of the most active cytotoxic agents in the treatment of cancer and has adverse side effects such as nephrotoxicity and hepatotoxicity. The present study was designed to determine the effects of royal jelly (RJ) against oxidative stress caused by CDDP injury of the kidneys and liver, by measuring tissue biochemical and antioxidant parameters and investigating apoptosis immunohistochemically. Twenty-four Sprague Dawley rats were divided into four groups, group C: control group received 0.9% saline; group CDDP: injected i.p. with cisplatin (CDDP, 7 mg kg(-1) body weight i.p., single dose); group RJ: treated for 15 consecutive days by gavage with RJ (300 mg/kg/day); group RJ + CDDP: treated by gavage with RJ 15 days following a single injection of CDDP. Malondialdehyde (MDA) and glutathione (GSH) levels, glutathione S-transferase (GST), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activities were determined in liver and kidney homogenates, and the liver and kidney were also histologically examined. RJ elicited a significant protective effect towards liver and kidney by decreasing the level of lipid peroxidation (MDA), elevating the level of GSH, and increasing the activities of GST, GSH-Px, and SOD. In the immunohistochemical examinations were observed significantly enhanced apoptotic cell numbers and degenerative changes by cisplatin, but these histological changes were lower in the liver and kidney tissues of RJ + CDDP group. Besides, treatment with RJ lead to an increase in antiapoptotic activity hepatocytes and tubular epithelium. In conclusion, RJ may be used in combination with cisplatin in chemotherapy to improve cisplatin-induced oxidative stress parameters and apoptotic activity.
The, Effect of Perforator Location on Epigastric Perforator Flap Survival: an Experimental and Stereological Study in Guinea Pigs
(Thieme Medical Publ inc, 2008) Tan, Onder; Ergen, Duygu; Atik, Bekir; Gundogdu, Cemal; Calik, Ilknur; Can, Ismail
One challenge most often seen in perforator-based flaps is the topographic relationship between the flap and its perforator, which determines flap design and pedicle length. Thirty female guinea pigs were used in this study. They were divided into four different groups including three experimental groups (n=8), which were designed as central, lateral, and distal groups according to the perforator location, and one control group (n = 6). Flap survival and vessel density rates were assessed. There was no statistically significant difference (p > 0.05) among either the surviving skin areas or the vascular density rates of the experimental groups, although all flaps were necrosed in the control group. We concluded that perforator flaps can safely be raised on the perforators located very distal or lateral to the flaps, as well as central classical location. Moreover, perforator flaps larger than suggested can safely be harvested in the same donor sites.