Browsing by Author "Celik, Hasan"

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Anom Approach for the Statistical Evaluation of Organic Acid Contents of Clones of the Grape Variety 'kalecik Karasi
(Hohere Bundeslehranstalt & Bundesamt Wein-& Obstbau Klosterneuburg, 2021) Keskin, Nurhan; Kunter, Birhan; Celik, Hasan; Kaya, Ozkan; Keskin, Siddik
Organic acids have an important effect on flavor, aroma and color in berries and wines of grapes and they also play an important role for the microbiological and biochemical stability of the wine. The variety 'Kalecik Karasi' is one of the exclusive red grape varieties of Turkey. The aim of this study is to evaluate and visualize the differences between the 23 clones of 'Kalecik Karasi' with respect to organic acids. As organic acids were considered: tartaric, malic, citric, succinic and fumaric acids. The berries of the vintages 2016 and 2017 were harvested with 23 % soluble solids and HPLC (High Performance Liquid Chromatography) was used to identify organic acid content. Using Analysis of Means (ANOM) method the mean of each group was compared to the overall or grand mean to identify statistically significant differences. Thus the differences between the 'Kalecik Karasi' clones in terms of organic acids were compared and visualized by this method. According to the results, for all organic acids, except for tartaric and fumaric acid, differences between means of clones and overall mean are statistically significant (p<0.05). Both ANOVA and ANOM can be appropriate for determining differences between the groups. However, ANOM provides a simple graphical representation for group means. Therefore, it can be concluded that this method can be suggested to visualize differences between the groups for easy interpretation.
Clonaltrans-Resveratrol Potential in the Ripened Grapes Ofvitis Vinifera L. Cv 'kalecik Karasi
(Springer, 2020) Keskin, Nurhan; Kunter, Birhan; Celik, Hasan
In this study,trans-resveratrol (t-RSV; 3,5,4 '-trihydroxy-trans-stilbene) contents of the ripened berries of 23 clones of 'Kalecik Karasi' which is one of Turkey's leading red wine grape cultivars, grown in Ankara (Kalecik) were determined by HPLC. For this purpose; the berries were collected at 22-24 degrees Brix during 2016-2017 vintage seasons. Descriptive statistics fort-RSV content of clones were expressed as mean with their standard errors. One-way analysis of variance was performed for the comparison. Duncan multiple comparison test was used to determine the significant variances among clones. In addition, Analysis of Mean (ANOM) was also applied to determine the differences of the clones from the grand mean. As a result,t-RSV content of the clones ranged from 0.31 mg kg(-1)(Clone 22) to 1.67 mg kg(-1)(Clone 7) followed by Clone 6 with 1.12 mg kg(-1)and differences among the clones were statistically significant (p < 0.05).
Evaluation of Clonal Variability of Berry Phenolics in Vitis Vinifera L. Cv. Kalecik Karasi
(Springer, 2022) Keskin, Nurhan; Kunter, Birhan; Celik, Hasan; Kaya, Ozkan; Keskin, Siddik
In clonal grapevine populations, genetic factors may have a significant effect on the amount of phenolic compounds in the grape berries. Thus, the capacity of the clones to produce distinctive chromatic profiles can be improved. This paper describes the phenolic contents and composition of grape berries as well as relationships among them for Kalecik Karasi clones to reveal their wine quality potentials. Seven individual polyphenols were quantified using high-performance liquid chromatography. The clones showed a significant difference (5.01 mg kg(-1) protocatechuic acid and 18.80 mg kg(-1) gallic acid) in berry phenolic compounds. Cluster analysis and multidimensional scaling were performed, and results showed that clones were clustered into three groups regarding phenolic compounds in the berries. Based on the phenolic compounds, 18 of the 23 clones were clustered into a group. Clones 16, 13, 8, and 2 were grouped together, while clone 7 was separated from the others. Including and excluding clone 7, approximately 40% phenotypic variation and 80% similarity were observed in 'Kalecik Karasi' clones, respectively. There were positive correlations between clones 2, 6, 7, 9, and 13 and p-coumaric, ferulic, gallic, and protocatechuic acids, as well as between clones 3, 5, 10, 14, 15, 34, 16, 19, and 20 and q-coumaric, vanillic, and syringic acid contents. Thus, it can be stated that multivariate methods can be used for clonal selection, and exclusive clones can be selected with high values of phenolic compounds in the future.
A Study on Total Phenolics and Vitamin C Contents of Kalecik Karasi (Vitis Vinifera L.) Clones
(Univ Agriculture, Fac veterinary Science, 2014) Keskin, Nurhan; Celik, Hasan; Kunter, Birhan; Keskin, Siddik
In this study total phenolic and vitamin C contents of the fully ripe berries of 23 clones of Kalecik Karasi which is one of the leading Turkish local red-wine grape cultivar originally grown in Kizilirmak valley near Kalecik/Ankara region were examined under the clone selection project supported by TUBITAK (Project Nr: 107 O 731). High-pressure liquid chromatography (HPLC) was used for vitamin C and spectrophotometer for total phenolics estimation. One way ANOVA was used to compare means of clone for their total phenolic and vitamin C contents. In addition to this univariate method, hierarchical cluster analysis was performed to identify similarity levels among the clones by considering total phenolics and vitamin C content together. Differences among the clones were found statistically significant for both characteristics. Total phenolic contents of the clones varied from 3.310 mg (clone 21) to 3.389 mg (clone 6) as GAE g fw. Vitamin C content ranged from 14.010 mg (clone 6) to 16.500 mg (clone 19) in 100g fw. Furthermore, similarity level for all clones was 83.1% that means variation rate is about 17% among the clones. As a summary of whole data, the first three performing clones are 6 (3.389 mg), 10 (3.374 mg) and 1 (3.365 mg) for total phenolics, and 19 (16.500 mg), 9 (16.020 mg), and 21 (16.015 mg) for vitamin C contents of the berries.