Browsing by Author "Celik, Mustafa"

Now showing 1 - 4 of 4

The Effects of Uv Irradiation and Incubation Time on in Vitro Phenolic Compound Production in 'karaerik Grape Cultivar
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2020) Celik, Mustafa; Keskin, Nurhan; Ozdemir, Fethi Ahmet
In this study, the effect of Ultraviolet (UV) irradiation on induction of individual and total phenolics production on callus cultures of 'Karaerik' grape cultivar was investigated. Callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. As a culture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were sub-cultured twice with 21 days intervals. After the second subculture, 12-day-old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Phenolic compounds were measured at 24th, 48th and 72nd hours. Individual phenolic compounds were analyzed by HPLC (High Pressure Liquid Chromatography) and total phenolic compounds were measured by spectrophotometer. As a result of the study, it was found that UV irradiation was effective for induction the production of phenolic compounds in the callus tissues of 'Karaerik' grape cultivar and this effect was closely related to the application time.
Evaluation of Acute Stress Disorder Following Pregnancy Losses
(Cukurova Univ, Fac Medicine, 2015) Keten, Hamit Sirri; Gencoglan, Salih; Dalgaci, Ahmet Ferit; Avci, Fazil; Satan, Yilmaz; Olmez, Soner; Celik, Mustafa
Purpose: The aim of this study was to evaluate acute stress disorder in women experiencing a pregnancy loss. Material and Methods: The study included 170 pregnant women presenting to the Obstetrics and Gynecology Clinic in research and training hospital of Kahramanmaras Sutcu Imam University between February 2013 and July 2013. Fifty-four women experiencing a pregnancy loss before the 21st week of gestation (31%) were assigned into the pregnancy loss group and 116 women whose pregnancy continued without any problems (69%) were assigned into the control group. Data were collected with Posttraumatic Stress disorder Scale (PTSDS) and a questionnaire composed of questions about socio-demographic features and history of pregnancy and diseases.. Results: The mean ages of the pregnancy loss group the control group were 28.83 +/- 7.30 years and 28.87 +/- 5.87 years respectively without a significant difference (p=0.968). The mean score for PTSDS was 32.40 +/- 10.24 (min: 17 and max: 52) in all the participants, 35.83 +/- 11.51 in the pregnancy loss group and 30.81 +/- 9.21 in the control group. The women experiencing a pregnancy loss had significantly higher scores for PTSDS. Conclusion: The study revealed that symptoms of posttraumatic stress disorder increased after pregnancy losses. It is of great importance to provide social and psychological support for the couples experiencing a pregnancy loss in terms of their wellbeing. Inability to implement support mechanisms effectively in cases of increased acute stress such as pregnancy loss can predispose to progression to posttraumatic stress disorder.
Evaluation of Adult Attention Deficit Hyperactivity Disorder in Smokers and Maras Powder Users
(Taylor & Francis Ltd, 2015) Keten, Hamit Sirri; Onay, Hakan; Ersoy, Ozgur; Isik, Oguz; Gencoglan, Salih; Ucer, Huseyin; Celik, Mustafa
Background: The aim of this study was to evaluate adult attention defi cit and hyperactivity disorder (ADHD) in smokers, Maras powder users and non-users of tobacco products by using the Adult Attention Defi cit and Hyperactivity Disorder Self-report Scale (ASRS). Methods: The study was performed on 446 males presenting to family health centers in Kahramanmara, Turkey. Of 446 participants, 104 were Maras powder users, 133 were smokers and 209 were volunteers not using any tobacco products. Data were collected with a questionnaire composed of questions about socio-demographic features and features of smoking and Maras powder use, and the Fagerstrom Nicotine Dependence Scale and the ASRS. P < 0.05 was considered signifi cant. Results: The mean age of the participants was 33.94 +/- 10.68 years (min = 18, max = 64). There was no signifi cant difference in age between the study groups (P = 0.089). Thirty-one participants (7.0%) received scores higher than the cut-off value (>= 14 points) for the short form of the ASRS. Twenty-one participants (4.7%) achieverd scores higher than the cut-off value (>= 44 points) for the ASRS. The smokers received the highest scores of 8.42 +/- 3.79 for all the subscales of the short version of the ASRS, 26.87 +/- 10.46 for the ASRS, 12.57 +/- 6.47 for the attention defi cit subscale and 14.30 +/- 5.35 for the hyperactivity and impulsivity subscale. Conclusion: Higher rates of the smokers and the Maras powder users had symptoms of ADHD. Considering nicotine may decrease symptoms of ADHD, it can be assumed that the rates of the smokers and Maras powder users with ADHD are higher.
Study of Phylogenetic Relationship of Turkish Species of Matthiola (Brassicaceae) Based on Issr Amplification
(Tubitak Scientific & Technological Research Council Turkey, 2016) Dogan, Bekir; Celik, Mustafa; Unal, Murat; Sefali, Abdurrahman; Martin, Esra; Kaya, Ayla
Matthiola W.T. Aiton is a taxonomically complex genus in which there are many problems, mostly with Matthiola longipetala and M. odoratissima. Matthiola species native to Turkey were collected from various locations in Anatolia, and their DNA was isolated. Revision studies performed on the basis of molecular data obtained from studies conducted in recent years have made the phylogenetic relationships and systematic positions of the taxa more apparent and reliable. Consequently, the remaining taxonomic problems among the species have been resolved through the use of DNA-based molecular analysis methods, which, unlike phenotype studies, are not affected by environmental factors. The inter-simple sequence repeat (ISSR) fingerprinting method was used in the study because its properties were considered to be more reliable and consistent than those of the randomly amplified polymorphic DNA method. DNA fragments were amplified through the use of ISSR primers. The phylogenetic relationships among the taxa were represented on a dendrogram constructed through means of NTSYSpc 2.02 software. The infrageneric and intergeneric phylogenetic relationships between Matthiola and other related genera were also characterized. It was determined that the taxa Matthiola odoratissima and M. ovatifolia are separate but closely related. Moreover, it was observed that the Matthiola longipetala complex forms a separate group within the genera. Clearly, the genera Matthiola, Sterigmostemum, Strigosella, Malcolmia, and Chorispora are phylogenetically differentiated on the dendrogram.